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Citations for Merck :
901 - 950 of 3190 citations for Acetamide N 5 bis 2 hydroxyethyl amino 2 2 chloro 4 6 dinitrophenyl azo 4 methoxyphenyl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The detergent-free preparation was dried down and reconstituted in 20 µl of 2% acetonitrile (ACN, Merck), 0.1% formic acid (FA ...
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bioRxiv - Physiology 2024Quote: ... Fluorescence-labeled polystyrene microspheres (latex beads) were bought as 2% or 2.5% solutions from Merck (Taufkirchen, Germany) and Thermo Fisher Scientific (Langenselbold ...
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bioRxiv - Developmental Biology 2024Quote: ... and 0.43 mM MgCl2) with the addition of 0.2 mM 1-phenyl-2-thiourea (PTU, Merck, Germany) to block pigmentation ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: For GC-MS analysis all the samples were derivatized with N,O- Bis(trimethylsilyl) trifluoroacetamide with 1% trimethylchlorosilane (BSTFA) purchased from Merck (Darmstadt, Germany).
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bioRxiv - Molecular Biology 2021Quote: ... was centrifuged at 1500 rpm for 10 mins and the supernatant was used to measure inflammatory cytokines (IL-4, IL-5, IL-13, γ-INF) by LUMINEX multi-factor detection (MERCK). The cell pellets were fixed in 4% formaldehyde for Wright-Giemsa staining and total cells were counted and classified in each slide.
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bioRxiv - Systems Biology 2020Quote: TGF-β/Activin/Nodal signal inhibitor (SB) stock (4–5 mM): SB 431542 hydrate (#S4317-5MG, Merck & Co., Inc., NJ, USA) diluted in DMSO (#D2650-5×5ML ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were centrifuged (1500 g for 5 min at 4°C) and resuspended in 3 x PCV buffer A + 0.1% NP40 (Merck Life Science). After 10 further min on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... After 30 minutes of incubation 100 μL of 2X STOP buffer (NaCl 340 mM, EDTA 20 mM, EGTA 4 mM, 5% Digitonin, 100 μg/mL RNase A (Merck, R6513), 50 μg/mL Glycogen (Thermo Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Elution sample was concentrated at 3,300 rpm for 5 minutes per round until it reached 500 uL with Amicon Ultra-4 30K centrifugal filter system (Merck Millipore). Size exclusion chromatography was performed with Superdex200 increase 10/300 column (Cytiva ...
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bioRxiv - Developmental Biology 2022Quote: ... GHCre/iDTR mice and Cre-negative control littermates (further referred to as -/iDTR) (postnatal day (PD) 4) were intraperitoneally (i.p.) injected with 4 ng diphtheria toxin (DT; Merck, Darmstadt, Germany) per g bodyweight ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were loaded onto self-made 4–13% or 4-10% polyacrylamide gradient gels separated and transferred onto PFDV membranes (Merck Millipore) followed by detection by specific antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... with PBS pH 7.4 for 10 min at 4400 x g and 4°C using an Amicon Ultra-4 concentrator with 3 kDa cutoff (Merck Millipore). The degree of labelling (DOL = 1.96 ...
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bioRxiv - Neuroscience 2022Quote: ... Unencapsulated molecules were removed by washing at least 4 times the LPs suspensions with PBS pH 7.4 for 20 min at 4°C and 5000 x g using an Amicon Ultra-4 concentrator with 100 kDa cutoff (Merck Millipore). The degree of encapsulation was calculated by subtracting from the total mass of the molecule to be encapsulated that present in the flow-through and was found to be higher than 75% in all LP preparations.
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bioRxiv - Cancer Biology 2024Quote: ... or expressing the sequence of 4-1BBL (Vector Builder, pLV[Exp]-Hygro-EF1A>4-1BBL) using X-tremeGENE™ HP DNA Transfection Reagent (Merck).
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bioRxiv - Microbiology 2024Quote: ... supplemented with 0.1% Tween-20 (PBS-T) and blocked using PBS supplemented with 4% w/v skimmed milk powder (PBSM-4%; Merck Millipore) for 1h at room temperature (RT) ...
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were permeabilized with 0.01% saponin (10 min, 4°C) and labeled with Phalloidin-TRITC (Merck, 30 min, 4°C). After washing ...
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bioRxiv - Cancer Biology 2024Quote: ... Chromatin was pelted at 2000 rcf for 4 mins at 4°C and resuspended in the RIPA buffer (Merck,20-188). The concentration of protein was determined using the Pierce BCA protein assay kit (Thermofisher) ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were separated using mPAGE 12% Bis-Tris gels (Merck; 5 μl loading volume per sample) with MOPS running buffer (50 mM MOPS ...
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bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat. No 71403). Protein expression was always performed using freshly transformed chemically competent E ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were seeded (2×104 cells/well) in 24-well plates in alphaMEM medium (Merck, Darmstadt, Germany, #F0925) plus 10 % FCS (Gibco ...
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bioRxiv - Biophysics 2021Quote: ... for 2 hours at 37°C and purified using an Amicon 30 kDa MWCO filter (Merck, Darmstadt, Germany). The Deep Vent exo- DNA polymerase (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: ... anti-RNA polymerase II subunit B1 (phospho CTD Ser-2) clone 3E10 (Merck, 04-1571-I, 1:5,000), and anti-RNA polymerase II CTD repeat YSPTSPS antibody clone 8WG16 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed 2 twice with PBS and were permeabilized with PBS–0.06%-Triton X-100 (Merck, USA) for 10 min at RT ...
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bioRxiv - Biophysics 2022Quote: ... for 2 hours at 37°C and purified using an Amicon 30 kDa MWCO filter (Merck, Darmstadt, Germany). Deep Vent exo-DNA polymerase (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: AKT1/2 phosphorylation was inhibited with InSolution™ Akt Inhibitor VIII (1-10 µM, Merck KGaA, Darmstadt, Germany) in FCS-free medium for 3 hours prior to treatment with HGF (10 ng/µl).
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bioRxiv - Systems Biology 2022Quote: ... 70 kDa FITC-dextran and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) were purchased from Merck Life Science UK Ltd. ...
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bioRxiv - Biophysics 2022Quote: Purified FHL was concentrated to 1-2 mg/mL using an Amicon centrifugal concentrator (100-kDa cutoff; Merck) and 3 µL of protein solution was applied to freshly glow-discharged C-flat 1.2/1.3 or 2/1 holey carbon grids (Protochips) ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were dissolved in 2% acetonitrile containing 0.1% trifluoroacetic acid and desalted using C18 ZipTips (Merck Millipore, Germany). Each sample was independently analysed on a Q-Exactive hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Prior to sterilization pH of ASW was calibrated at 8.5 ± 0.5 through the addition of 2 M NaOH (Merck) solution ...
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bioRxiv - Physiology 2020Quote: ... following which they were incubated with anti-MAP-2 antibody (AB5543, 1:500, Merck Millipore, Burlington, MA, USA) in blocking buffer overnight at 4℃ ...
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bioRxiv - Molecular Biology 2021Quote: ... thawed pellet was resuspended into 20 ml lysis buffer (0.025 M Tris pH 8; 0.5 M NaCl; 2 mM MgCl2; 100 U/ml Benzonase (Merck); 0.25 mg/ml lysozyme (Roche) ...
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bioRxiv - Microbiology 2020Quote: Cytotoxicity of the compounds were tested by use of a MTT [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide] assay (Merck) at a final MTT concentration of 0.45 mg/ml or by use of a ATPlite Luminescence Assay System (PerkinElmer ...
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bioRxiv - Cell Biology 2022Quote: ... cells pre-labeled with transferrin-Alexa Fluor 594 were treated for 2 hours with 10 μM SAR405 (Merck, cat ...
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bioRxiv - Microbiology 2022Quote: ... concentrated to 2-10 mg/ml using Amicon concentration devices (Merck, 3,000 or 10,000 Da cut-off filter) and stored in aliquots at −80 °C until needed ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed three times in 0.1 M sodium cacodylate buffer pH 7.2 ± 0.1 containing 0.2 M sucrose and 2 mM MgCl2 (Merck Millipore), and adhered to 12 mm diameter round glass coverslips (Paul Marienfeld GmbH & Co ...
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bioRxiv - Cell Biology 2022Quote: ... Pooled eluates were then concentrated approximately 40 times using a centrifugal filter (Amicon Ultra-2 10k, UFC201024, Merck Millipore ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Human HepaRG hepatoma cells (1.5-2×105 cells/cm2, Biopredic International, Rennes, France) were cultured in William’s E medium (Merck) supplemented with 10% heat-inactivated FCS ...
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bioRxiv - Zoology 2024Quote: ... The sample was then placed in 2 ml petroleum ether (boiling range 40-60°C, Merck, Darmstadt, Germany) at room temperature for two days ...
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bioRxiv - Neuroscience 2024Quote: ... Total 2 mg lysate (1 ml) was incubated with 10 µl of anti-FLAG M2 Magnetic Beads (Merck), which was pre-washed twice with IP buffer ...
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bioRxiv - Cell Biology 2024Quote: ... L-glutamine and antibiotic/antimycotics and decidualized with 1µM PGE2 or 1µM relaxin-2 (accession # P04090) (both Biotechne Ltd, Abbington, U.K.) in combination with 1µM medroxyprogesterone acetate (MPA) (Merck) for up to 4 days ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 2 × 104 cells were cultured in an 8-well chamber slide (Merck Millipore, Massachusetts, USA) and then fixed with 4% paraformaldehyde in PBS buffer for 15 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the mice were inoculated with 100,000 SB28-GFP cells suspended in 2 µl of DMEM (Merck, Darmstadt, Germany) (glioblastoma mice ...
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bioRxiv - Molecular Biology 2023Quote: Cells of 2-3 days growth with approximately 70-90% confluency have been treated with Bortezomib (Merck, #504314) at 100 nM for 42 h ...
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bioRxiv - Microbiology 2023Quote: ... auris cells (CHRIST Alpha 1-2 LD plus lyophilizer, Osterode, Germany) using Tri Reagent (Merck Ltd. Budapest, Hungary). The quality of RNA was determined using the Eukaryotic Total RNA Nano kit (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then induced for EmGFP-NSD3 expression with 2 μg/ml of doxycycline (D9891-1G, Merck) for 48 h ...
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bioRxiv - Microbiology 2023Quote: ... A fraction of the inoculum was directly transferred into 2 ml tube containing TRI reagent (Sigma-Aldrich, Merck) and silica beads (0.1 mm diameter ...
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bioRxiv - Biophysics 2023Quote: ... 5 and 10 μl of the lipid extract 2 cm from the bottom on aluminum-backed silica gel 60 TLC plates (cat#: 1.05553.0001; Merck) together with DOPG ...
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bioRxiv - Molecular Biology 2022Quote: ... eggs rafts were hatched in trays containing 2 L of Milli-Q water (Synergy® UV, Merck, Germany). Larvae were fed continuously until the pupae stage with TetraMin® baby fish food (Tetra ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were stopped with 2 µl of 0.5 M EDTA and separated using thin layer chromatography plates (Merck) with 0.3 M LiCl and 0.3 M formic acid as the mobile phase ...
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bioRxiv - Biochemistry 2023Quote: The reaction mixtures (0.5, 1 or 2 µL) were spotted onto TLC Silica Gel 60 F254 plates (Merck). As for analysis of cyclization activity ...