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Citations for Merck :
901 - 950 of 4934 citations for 1 Piperidin 4 yl azetidine 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Endogenous peroxidases were blocked with 3% (v/v) H2O2 (Merck) in methanol (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... concentrated to ∼250 μL (Merck, Amicon, 3 kDa cut-off), were combined with 250 uL lysis buffer (1% CHAPS ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then fixed with 3% glutaraldehyde (Merck, Germany) in sodium phosphate buffer (0.1 M ...
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bioRxiv - Genetics 2021Quote: ... 33 mM 1,2-diheptanoylsn-glycero-3-phosphocholine (DHPC by Merck) and 1 % w/v sodium deoxycholate (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 3-nitro-L-tyrosine [5 µM] (Merck, Darmstadt, Germany) as internal standard (ISTD ...
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bioRxiv - Cell Biology 2020Quote: ... using 3 kDa MW cut-off filter units (Merck Millipore). In order to detect and quantify the amount of NGF ...
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bioRxiv - Biochemistry 2022Quote: ... 3 mg/mL (∼40 µM) using Amicon 10K concentrators (Merck) and stored at -80°C till use.
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bioRxiv - Biophysics 2022Quote: ... We used Amicron ultracel-3 centrifugal filters (Merck Millipore Ltd) with 3-kDa molecular weight cut-off ...
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bioRxiv - Neuroscience 2022Quote: ... was combined with 90μM sygRNA (5’ - GGATTTGGTAATAGCAG AGGGGG 3’) (Merck) at RT for 15 minutes to form an RNP complex ...
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bioRxiv - Developmental Biology 2024Quote: ... the TRPC3 inhibitor Pyr3 (Merck, 3 μM, 15-min incubation); or the TRPC6 inhibitor SAR-7334 (Selleck ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... and n-butanol (nBuOH) (3 × 100 mL each, Merck, China). The extract of each phase was concentrated in vacuo ...
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bioRxiv - Cancer Biology 2023Quote: ... in 3 % bovine serum albumin (BSA) (Merck, A3059, Darmstadt, Germany) for 20 min ...
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bioRxiv - Evolutionary Biology 2023Quote: Zygotes were de-jellified using 3 % cysteine (Merck Millipore, USA), washed 6 times in N ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA oligonucleotide G4A4 (5’-AAAAAAGGGGAAAAGGGGAAAAGGGGAAAAGGGGAAAAAA-3’) was purchased from Merck. CD analysis of 2,5 µM RNA was carried out in the buffer used for G4-pulldown ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue slides were blocked with a 3% BSA (Merck, A7906) and 10% Donkey serum (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... peroxidase quench with 3% hydrogen peroxide (Merck, New Jersey, USA), protein block with Dako protein block solution (Dako ...
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bioRxiv - Cell Biology 2023Quote: ... in 2× saline sodium citrate (SSC; Merck, 6132-04-3)) for 5 min at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... an inhibitor of the HIS3 reporter gene (3-AT; Merck), were added to avoid leakage of the HIS3 gene (Castrillo et al. ...
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bioRxiv - Bioengineering 2024Quote: ... mouse anti-Glyceraldehyde 3-phosphate dehydrogenase (GAPDH)-peroxidase (69295, Merck); anti-rabbit immunoglobulin G (IgG) ...
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bioRxiv - Genetics 2024Quote: ... 33 mM 1,2-diheptanoylsn-glycero-3-phosphocholine (DHPC by Merck) and 1% w/v sodium deoxycholate (Sigma) ...
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bioRxiv - Biophysics 2024Quote: ... Casein substrate (CAS: 9005-46-3) was bought from Merck and EnzChek casein BODIPY from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... which were subsequently functionalized using 3-aminopropyl triethoxysilane (APTES, Merck). For this ...
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bioRxiv - Plant Biology 2020Quote: About 8,000 T4 seeds from the W29-1-4 reporter line were mutagenized by treatment with 0.3% (v/v) ethylmethanesulfonate (Merck KGaA, Frankfurter, Darmstadt, Germany) for 15 h at 25°C ...
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bioRxiv - Cell Biology 2021Quote: ... were applied for one hour at room temperature and cells were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; Merck G8294; 1:3000) before mounting with Fluoromount™.
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C followed by incubation with primary antibody: anti-tyrosine hydroxylase (TH, 1:1000, Merck Millipore, Darmstadt, Germany, AB152). After washing with TBST ...
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bioRxiv - Biochemistry 2022Quote: ... Nuclei were stained with DAPI (1:1000 in PBS, AppliChem, Darmstadt, Germany) and coverslips were mounted on Mowiol 4-88 (Calbiochem, Merck Millipore, Billerica, Massachussetts) with the addition of 2,5% 1,4-Diazobicyclo[2.2.2]octan (Dabco ...
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bioRxiv - Immunology 2023Quote: ... The embryos were subsequently incubated overnight at 4 °C in solution containing Cy3-conjugated α-smooth muscle actin monoclonal antibody (1:100, C6198; Merck, Darmstadt, Germany), Alexa647-Phalloidin (1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were placed in a humid chamber and cells were incubated overnight at 4°C with the following primary antibodies diluted in blocking solution: mouse monoclonal anti-FLAG clone M2 (Merck F1804; 1:1000), rabbit monoclonal anti-KTN1 clone D5F7J (Cell Signaling Technology #13243 ...
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bioRxiv - Microbiology 2023Quote: ... slick SSW and non-slick SSW from sites #1 – #3 (Fig. 1b) were fixed with 25 % glutardialdehyde (0.5 % final concentration, Sigma-Aldrich/Merck Life Science AB, Solna, Sweden) and stored at −80 °C ...
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bioRxiv - Immunology 2024Quote: ... we included αTIM-3+ αPD-1 and used anti-human RSV-IgG4 as an isotype control antibody (5 μg/mL, 60AGK S228P, Merck & Co., Inc., Rahway, NJ, USA). On day six ...
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bioRxiv - Developmental Biology 2022Quote: ... GHCre/iDTR mice and Cre-negative control littermates (further referred to as -/iDTR) (postnatal day (PD) 4) were intraperitoneally (i.p.) injected with 4 ng diphtheria toxin (DT; Merck, Darmstadt, Germany) per g bodyweight ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were loaded onto self-made 4–13% or 4-10% polyacrylamide gradient gels separated and transferred onto PFDV membranes (Merck Millipore) followed by detection by specific antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Unencapsulated molecules were removed by washing at least 4 times the LPs suspensions with PBS pH 7.4 for 20 min at 4°C and 5000 x g using an Amicon Ultra-4 concentrator with 100 kDa cutoff (Merck Millipore). The degree of encapsulation was calculated by subtracting from the total mass of the molecule to be encapsulated that present in the flow-through and was found to be higher than 75% in all LP preparations.
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bioRxiv - Cancer Biology 2024Quote: ... or expressing the sequence of 4-1BBL (Vector Builder, pLV[Exp]-Hygro-EF1A>4-1BBL) using X-tremeGENE™ HP DNA Transfection Reagent (Merck).
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were permeabilized with 0.01% saponin (10 min, 4°C) and labeled with Phalloidin-TRITC (Merck, 30 min, 4°C). After washing ...
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bioRxiv - Cancer Biology 2024Quote: ... Chromatin was pelted at 2000 rcf for 4 mins at 4°C and resuspended in the RIPA buffer (Merck,20-188). The concentration of protein was determined using the Pierce BCA protein assay kit (Thermofisher) ...
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bioRxiv - Cancer Biology 2020Quote: ... and further filtered to a final volume of 1 ml by repeated centrifugation (4000 × g, 4°C) using an Amicon Ultra-15 spin-filter unit (Merck Millipore, MWCO = 100 kDa). The sample was then loaded onto a 24 ml SEC column packed with sepharose CL-4B ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The staining of the precipitated polypeptide-antibody complexes was performed by addition of 60 mg 4-chloro-1 naphtol (Merck/Sigma-Aldrich; Cat#C8890) in 20 ml ice-cold methanol to 100 ml phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... purified DNA from both input and ChIP fractions was diluted 1:4 in water and 1 μL was used for qPCR using a SYBR® Green JumpStart™ Taq ReadyMix™ (CAT S4438, Merck, UK) and a BioRad CFX96 instrument ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μL of this complex was added to a mixture of 15 μL of TIRF-Casein buffer supplemented with 1 μL each of an oxygen scavenging system (4 mg/mL catalase, Merck; and 30 mg/mL glucose oxidase, Merck, dissolved in TIRF buffer), 45% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... Total protein stain (30% methanol, 6.7% acetic acid, 0.0005% Fast Green FCF (Merck KGaA, Darmstadt, Germany)) stain was used as a loading control and imaged prior to blocking ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA:DNA hybrids were immunoprecipitated from total nucleic acids using 5µg of S9.6 antibody (MABE1095, Merck Millipore) in binding buffer (10mM Na2HPO4 pH 7.0 ...
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bioRxiv - Systems Biology 2021Quote: ... 100 μl of glass beads (0.5 mm, acid-washed, cat no. G8772, Merck KGaA, Darmstadt, Germany) were added to each well using a 96-well bead dispenser (LabTIE International ...
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bioRxiv - Systems Biology 2022Quote: ... Peptide fractions were eluted using MeOH buffer containing trifluoroacetic acid (TFA) (Merck Sigma, Cat. No. 302031), FA or ABC ...
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bioRxiv - Neuroscience 2021Quote: 12 wells plates were coated o/n with poly-L-lysine (0.1 mg/ml [SigmaAldrich]) in 0.1 M borate buffer pH 8.5 (0.04 M Boric acid [Merck Millipore] ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
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bioRxiv - Developmental Biology 2020Quote: ... Signal was developed using an ECL substrate (100 mM Tris-HCl, pH 8.5, 0.2 mM coumaric acid, 1.25 mM luminol, 0.01% H2O2; all Merck) and exposed on X-ray films (Agfa) ...
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bioRxiv - Microbiology 2021Quote: ... The samples were deproteinized by addition of 10 μL of cold 5-sulphosalicilic acid (SSA, Merck) (300 mg/ml ...
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bioRxiv - Plant Biology 2020Quote: ... Analytical grade zinc acetate dehydrate (Zn(CH3COO)2.2H2O) and citric acid (C6H8O7) were purchased from Merck and used as received ...
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bioRxiv - Neuroscience 2021Quote: Commercial pectin derived from citrus peels (galacturonic acid, ≥74.0%, dried basis, Sigma-Aldrich, Merck, Darmstadt, Germany), labelled “Citrus” in Figures ...