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Citations for Merck :
851 - 900 of 4100 citations for Pregnanediol 3 Glucuronide PDG ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and then concentrated using 15 ml Amicon spin filter cartridges (cutoff: 3 kDa, 10705884-Merck Millipore, Burlington, MA, USA) by serial addition of 10 ml of CM and centrifugation at 4000 x g until 1.5 ml were left ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were incubated in primary antibodies solution (antibody diluted in PBS containing 3% goat serum, 0.01% Triton x-100 (Merck)) at 4°C over night ...
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bioRxiv - Biochemistry 2019Quote: ... using binding buffer and concentrated using a 3 kDa high molecular weight cut-off Amicon Ultra Centrifugal Filter (Merck). SDS-PAGE was used to assess the purity of untagged SbtB7001 ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μL of the bacteriocin sample was loaded onto the trap column at 3% (v/v) ACN (Merck, USA) containing 0.1% (v/v ...
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bioRxiv - Plant Biology 2021Quote: ... the supernatant was applied to a centrifugation filter (Amicon Ultra 0.5 mL 3 K device; Merck KGaA, Darmstadt, Germany), and the fraction containing free amino acids was obtained by centrifugation (15,000 × g ...
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bioRxiv - Biophysics 2021Quote: ... the dry lipid film was rehydrated with 3 mL of 0.2 M 8.5 pH Bicine (Merck Life Science, Norway) buffer (pH adjusted with NaOH (Merck Life Science ...
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bioRxiv - Cancer Biology 2022Quote: ... 400μL were mixed with 200μL of a 3 mg/mL collagen in 0.1% acetic acid solution (rat tail type I; Merck Millipore), followed by the addition of 10μL of 1 M NaOH ...
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bioRxiv - Cell Biology 2022Quote: ... cells were permeabilized with 0.1% Triton X-100 for 3 minutes and then blocked with 5 or 10% bovine serum albumin (BSA, Merck) in PBS for 20 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... with 3 mg/mL of bovine serum albumin (BSA) and 100 µM of IBMX (Sigma-Aldrich, Merck, Darmstadt, Germany), and collected ...
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bioRxiv - Immunology 2022Quote: ... approximately 5×105 Calu-3 cells were pre-treated with kp7-6 (100 ug/mL, CD95/CD95L antagonist, Merck) for 2 hour and then infected with SARS-CoV-2 at an MOI of 0.2 ...
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bioRxiv - Microbiology 2022Quote: ... and 10% ultra-pure water from Direct-Q 3 UV Water Purification System with LC-Pak Polisher (Merck KGaA) and mobile phase B was 10 mM ammonium acetate at pH 9 in ultra-pure water with 15 μM medronic acid (InfinityLab Deactivator additive ...
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bioRxiv - Molecular Biology 2022Quote: All samples were centrifuged at 16,000 × g for 30 min and 400 µL supernatant was loaded on a 0.5 mL centrifugal ultrafiltration unit with 3 kDa cut-off membrane (Amicon, Merck) and concentrated down to 20 µL ...
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bioRxiv - Immunology 2022Quote: ... For intracellular cytokine labeling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (10μg/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... The PDMS microstructures were then rinsed with 2:3 HEPES1/ethanol and with ultrapure water (Milli-Q, Merck-MilliPore). PVP (1.3 MDa ...
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bioRxiv - Biochemistry 2022Quote: ... The LE11-27 DNA was dialyzed against the same buffer and concentrated to ~ 2.5 mM (Vivaspin 500, 3 kDa MWCO, Merck). The complex was formed by mixing the protein and the DNA in a 2:1 protein-to-DNA ratio (final concentration of 800 µM and 400 µM ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated in the presence or absence of aphidicolin 2μM for 3 hours together with cytochalasin B (3μg/ml, Merck Millipore) to block cytokinesis prior to fixation for immunofluorescence.
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated and using using Amicon Ultra-15 centrifugal filters (3 kDa cut-off, Merck-Millipore) and then buffer was exchanged to buffer B (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was transferred to an Amicon Ultra-0.5 Centrifugal Filter Unit 3 kDa (Merck Millipore, Catalogue no. UFC500396) and centrifuged at 10,000 g for 20 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Japan) with a Discovery HS F5 column (2.1 mm i.d. × 150 mm, 3 μm particle size, Merck, Darmstadt, Germany) coupled with a Q Exactive instrument (PFPP-LC/MS ...
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bioRxiv - Bioengineering 2023Quote: ... The methanol-fixed samples were transferred to 3% glutaraldehyde solution (Agar scientific) in 0.1 M sodium phosphate buffer (Merck). Dehydration was performed with ascending ethanol series (30% ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were blocked for 3 hours in 5% milk in TBS (50 mM Trizma base and 150 mM NaCl, PH 8.3, both Merck) plus 0.05% Tween-20 (TBST ...
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bioRxiv - Immunology 2024Quote: ... Cell supernatant was then concentrated by Tangential Flow Filtration with a Pellicon 3 Ultracel 10 kDa membrane (Merck Millipore) and loaded onto a 10 mL CaptureSelect™ C-tagXL affinity column that had been equilibrated in Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2023Quote: ... rinsed in running tap water for 3 min and stained with 0.02% Fast Green for 30 seconds (F7252, Merck), followed by 1% acetic acid for 30 seconds and Safranin’O solution for 45 min (S2255 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-HES-1 (1:500, 1% casein, #AB5702, Millipore, Merck, Darmstadt, Germany), anti-LRP6 (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% glutamine and 1% penicillin/streptomycin (Merck). Fascin KD cells were selected and maintained using puromycin ...
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bioRxiv - Neuroscience 2020Quote: ... GluA1 (1:250-1:500; Merck Millipore) and RTP801 (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... The water used was of Milli-Q Advantage A10 as well as MultiScreen-HV 96-well Plates (Merck Millipore). Calibration ranges were 3.125 – 200 ng/ml for U and 25 - 500 ng/ml for UH2 and 5-FU (5μg/ml ...
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bioRxiv - Microbiology 2019Quote: ... Aliquots of neutral lipid extracts were analyzed by thin layer chromatography (TLC) on 60 F254 silica gel plates (Merck) using hexane/diethyl-ether/acetic-acid (90:8:1 ...
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bioRxiv - Immunology 2021Quote: ... The plates were then washed 5 times with HSWB followed by incubation of goat anti-mouse antibody (Merck, Germany) conjugated with HRP (diluted 1:3000 in LSWB + 1% BSA ...
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bioRxiv - Microbiology 2019Quote: ... The steroid standards and products were separated on silica gel-coated aluminum TLC plates (Silica gel 60 F254: thickness, 0.2 mm; 20 × 20 cm; Merck) using dichloromethane:ethyl acetate:ethanol (14:4:0.05 ...
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bioRxiv - Immunology 2021Quote: ... (final concentration of 2µg/mL) and pools of original or beta variant peptides on hydrophobic-PVDF ELISpot plates (Merck) coated with 5µg/mL anti-mouse IFNγ (AN18) ...
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bioRxiv - Biochemistry 2020Quote: ... Reaction mixtures were stirred using Teflon-coated magnetic stirrer bars and monitored by thin layer silica gel chromatography (TLC) using 0.25 mm silica gel 60F plates with fluorescent indicator from Merck. Plates were visualized under UV or treated by KMnO4 stain with gentle heating ...
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bioRxiv - Cell Biology 2021Quote: ... and/or with plasmid DNA (e.g. 1µg/well for 6-well plates) for 24 hrs using GeneJuice (Merck Millipore). siRNAs targeting USP7 ...
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bioRxiv - Microbiology 2021Quote: ... Polar lipids were separated by two-dimensional thin layer chromatography (TLC plates coated with silica gel, 1010 cm; Merck). Chromatography was performed using chloroform/methanol/water (65 ...
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bioRxiv - Cell Biology 2021Quote: ... Growth assays on solid medium were done as described (Schuck et al., 2009) using SCD plates with and without 0.2 µg/ml tunicamycin (Merck). Plates were imaged after 1.5 days of growth at 30°C.
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bioRxiv - Neuroscience 2022Quote: ... Cells were cultured on poly-L-ornithine and laminin-coated coverslips in 24-well plates at 150,000 cells per well in serum-free 2i Medium (Merck). After 24h ...
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bioRxiv - Cell Biology 2022Quote: ... and seeded in 48-well plates (3000-4000 cells/well) previously coated with 0.1mg/ml poly-D-lysine (2h, 37°C; Merck) and 2μg/ml laminin (over-night (O/N) ...
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bioRxiv - Biochemistry 2020Quote: ... was immobilized into the membrane pores (0.45 μm pore size) of a 96-well MultiScreen-IP polyvinylidene fluoride (PVDF) filter plate from Merck Millipore (Figure 2B) ...
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bioRxiv - Microbiology 2019Quote: ... The pellet was dissolved in three ul distilled water and spotted onto 5×5 cm silica gel plates (Merck) using automatic TLC sampler ...
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bioRxiv - Developmental Biology 2019Quote: ... Silica gel 230-400 mesh and silica gel coated plates Kieselgel 60 F254 were purchased from Merck (Darmstadt, Germany) and were used for flash-chromatography (FC ...
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bioRxiv - Microbiology 2020Quote: The TLC was performed by spotting 2 μL of enzymatic reaction on a silica gel 60 F454 plate (Merck), the separation was carried out in butanol ...
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bioRxiv - Microbiology 2019Quote: ... and 5 µl aliquots of the supernatants were applied to polyethyleneimine (PEI) cellulose thin layer chromatography plates (Merck, Sigma), resolved with 1.5 M KH2PO4 (pH 3.4 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Unreacted dye and SMA-SH were removed by thin layer chromatography (TLC) using silica gel plates (Merck, Darmstadt, Germany) and a mobile phase of chloroform/methanol/water (5:5:1 v/v/v) ...
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bioRxiv - Microbiology 2020Quote: ... after which the plates were placed inside a 2.5 L sealed jar (BBLTM GasPakTM from BD Biosciences or from Merck) under micro-aerobic conditions created by using an oxygen-absorbing AnaeroGen sachet (Oxoid) ...
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bioRxiv - Microbiology 2020Quote: ... room-temperature and slightly dried SabCG media plates and a sterile glass L-shaped bacteriology cell-spreader (Merck, S4522) was used to spread the liquid evenly over the entire surface and allowed to soak/dry before use.
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bioRxiv - Microbiology 2021Quote: ... An aliquot was subjected to TLC using a silica gel 60 F254 aluminum TLC plate (Merck Millipore, cat.no.105554,) with a running buffer containing ethyl acetate/acetic acid/methanol/formic acid/water in a ratio of 80:40:10:10:10 (v/v) ...
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bioRxiv - Microbiology 2019Quote: ... An inoculum from the SDA plate was suspended in 25 ml of Sabouraud dextrose broth (SDB; Merck, Darmstadt, Germany) and incubated for 18 h under agitation (120 rev/min ...
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bioRxiv - Microbiology 2020Quote: ... 96-well plates with confluent cell layers were fixed and virus was inactivated by immersion in 100% methanol (MERCK) for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... The dried pellets were dissolved in 80 % methanol and analysed by HPTLC on a Silica Gel 60 plate (Merck). Ethyl acetate ...
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bioRxiv - Microbiology 2021Quote: ... Five lactose positive colonies were selected form the plate and β-glucuronidase activity was confirmed using Chromocult Agar (Merck).