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Citations for Merck :
851 - 900 of 2598 citations for Centrosomal Protein 63 kDa CEP63 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The flow through and wash fractions were concentrated to 3-5 mg/mL using Amicon ultrafiltrators (10 kDa MWCO, Merck Millipore, Darmstadt, Germany). All fractions were combined and dialyzed 2x over night at 4 °C against 5 L 10 % (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins purified under native conditions were concentrated to 400 μL using a 10 kDa molecular weight cutoff (MWCO) Amicon Ultra-4 centrifugal spin concentrator (Merck-Millipore, Burlington, MA) and purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 column (Cytiva) ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant containing the asymmetric vesicles was then concentrated to < 500 μl using 15 ml-Amicon centrifuge filters (Merck, 100 kDa cut-off) at 5000 g ...
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bioRxiv - Developmental Biology 2022Quote: ... The antibody was washed three times and concentrated at 4 °C using Amicon Ultra-0.5 100 KDa centrifugal filter devices (Merck Millipore, cat. no. UFC100), which remove salts and preservatives (e.g ...
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bioRxiv - Biochemistry 2023Quote: ... The reactions were incubated at 30°C for 1 h before they were concentrated with an Amicon® Ultra-0.5ml Centrifugal Filter Unit (molecular weight cut off = 100 kDa; Merck Millipore, Billerica, USA). Subsequently ...
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bioRxiv - Immunology 2023Quote: ... The resulting Fabs were buffer exchanged into PBS and then concentrated down using Amicon® 10 kDa Ultra-15 Centrifugal Filter Units (Merck Millipore UFC9010). Selected fractions resulting from the size-exclusion chromatography were pooled together and concentrated again for later use.
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bioRxiv - Synthetic Biology 2023Quote: ... by centrifuging the purified enzyme preparations (4,000×g, 4°C) using AmiconTM Ultra-15 centrifugal filters of 10 kDa-pore size (Merck-MilliPoreSigma, Burlington, MA, USA). The absorbance at 280 nm (A280 ...
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bioRxiv - Bioengineering 2023Quote: ... Fractions 7 to 10 which were subsequently identified as the particle fractions (PF) were pooled to a volume of 2 ml and concentrated using an Amicon Ultra 100 kDa centrifugal filter (Merck Millipore, MA, USA), made from regenerated cellulose ...
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bioRxiv - Biochemistry 2023Quote: ... and relevant fractions were pooled and concentrated to > 36 mg/ml (416 µM) using an Amicon Ultra centrifugal filter with a 50 kDa MWCO (Merck Group, Darmstadt, Germany).
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bioRxiv - Biochemistry 2023Quote: ... and diluted with SEC buffer to a final concentration of 32 mg/ml (370 µM) using an Amicon Ultra centrifugal filter with a 50 kDa MWCO (Merck Group, Darmstadt, Germany). The protein solution was used directly for protein crystallization or stored for up to 10 days on ice ...
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bioRxiv - Neuroscience 2024Quote: ... The filtered supernatant was concentrated ×20 using an Amicon® Ultra-15 centrifugal filter device (molecular weight cut-off 100 kDa, UFC910024, Merck Millipore Ltd.), which was centrifuged at 4,000×g for 20 minutes at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... CHAPS was added to the dialyzed sample to concentration of 1% after which the MSP was concentrated to 20-40 mg/ml in centrifugal filter (Merck, MWCO 30 kDa), flash frozen and stored at -80°C.
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bioRxiv - Physiology 2022Quote: ... To detect total protein carbonylation by immunoblot we used the OxyBlot Protein Oxidation Detection kit (Merck) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were measured for protein concentration using a Non-Interfering protein Assay kit (cat#488250, Merck Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... Protein standards were obtained from Merck.
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... 2.0 Protein Detection System (Merck Millipore), incubated with Clarity Western ECL (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... after testing multiple antibodies (anti-NeuN rabbit Antibody, ABN78 & ABN78C3, Merck; anti-NeuN rabbit Antibody, ab177487, Abcam; anti-NeuN mouse Antibody, MAB377, Merck) and increasing antibody concentrations (up to 1:50) ...
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bioRxiv - Biochemistry 2020Quote: ... The enzyme was then removed via diafiltration by passing the mixture through a 30 kDa centrifugal filter device (Amicon®, Merck Millipore Ltd., Ireland). The absence of AtXOAT1 in the filtrate ...
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bioRxiv - Cancer Biology 2020Quote: ... and further filtered to a final volume of 1 ml by repeated centrifugation (4000 × g, 4°C) using an Amicon Ultra-15 spin-filter unit (Merck Millipore, MWCO = 100 kDa). The sample was then loaded onto a 24 ml SEC column packed with sepharose CL-4B ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... of 50 mM EDTA was added to the reaction mixture and unchelated radioactive copper ion was removed by centrifugation using Amicon® Ultra centrifugal filters (regenerated cellulose, 3 kDa) (Merck Millipore, Billericia, MA). The radioactivity of 64Cu complexed NOTA-POx was measured in gamma counter prior to micelle preparation.
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bioRxiv - Microbiology 2022Quote: ... the supernatants were concentrated 50-fold using Amicon Ultra-4 centrifugal filters with a molecular weight cut-off of 3 kDa (Merck-Millipore, Burlington, MA, USA). Samples were mixed with 3× Laemmli loading buffer and heated for 3 min at 85 °C before SDS-PAGE analysis ...
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bioRxiv - Biochemistry 2023Quote: ... AnfH2 eluted in a clear peak at around 205 mL and was subsequently concentrated using an Amicon® Ultra-15 Centrifugal Filter Unit (molecular weight cut off = 30 kDa; Merck Millipore, Billerica, USA). Protein yields for both nitrogenase component fractions were determined using the Quick Start™ Bradford 1x Dye Reagent (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged to remove cell debris and concentrated with Amicon Ultra-15 Centrifugal Filters (10 kDa molecular weight cut-off; Merck Millipore Ltd, Carrigtwohill, Ireland). Aliquots were stored at −80°C until usage.
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bioRxiv - Biochemistry 2023Quote: In situ protein-protein interaction was evidenced using Duolink® Proximity Ligation Assay (Merck, DUO920082, DUO92004, DUO92008). Cells were fixed with formaldehyde and permeabilized using ice-cold 90% methanol ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies: anti-LC3 antibody Atg8 (Merck Millipore #ABC975) (1:1000) ...
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bioRxiv - Genomics 2020Quote: ... The protein solutions were subsequently desalted and protein amount was estimated using Direct Detect cards (Merck Millipore, Darmstadt). All samples were freeze dried before further processing ...
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bioRxiv - Cell Biology 2023Quote: Protein carbonyl formation by oxidative reactions was assessed with the OxyBlot Protein Oxidation Detection Kit (S7150, Merck Millipore), according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... after 10 µM peptide was incubated for 30 min with or without LUVs (500 µM total lipid) in a 100 KDa MWCO Amicon Ultra® tube (Merck Millipore, Billericia, MA, USA), the sample was centrifuged at 9000 × g for 15 min ...
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... and eSpCas9-GFP protein (#ECAS9GFPPR, Merck KGaA) were introduced into MCF7 and T47D cells using Viromer® CRISPR (Lipocalyx GmbH ...
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bioRxiv - Evolutionary Biology 2020Quote: ... protein G-magnetic beads (Merck Millipore, LSKMAGG10) were used instead ...
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bioRxiv - Systems Biology 2022Quote: ... proteins were loaded to a filter (Merck-Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... Microtubule-Associated Protein 2 (MAP-2) (Merck Millipore Burlington ...
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bioRxiv - Neuroscience 2019Quote: ... in combination with cas9 nuclease protein (Merck) was used for gene editing ...
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bioRxiv - Cell Biology 2021Quote: ... in combination with cas9 nuclease protein (Merck) was used for gene editing ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were transferred onto PVDF membranes (Merck). The membranes were blocked with 5% defatted milk before blotted with primary antibodies and secondary antibodies [anti-mouse IgG-HRP (at 1:2,000 dilution) ...
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bioRxiv - Immunology 2019Quote: ... in combination with cas9 nuclease protein (Merck) was used for gene editing ...
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bioRxiv - Biophysics 2021Quote: ... proteins were transferred to nitrocellulose membranes (Merck). Nitrocellulose membranes were blocked with 5% (w/v ...
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bioRxiv - Plant Biology 2021Quote: ... separated proteins were transferred to PVDF (Merck) membranes for immunoblotting ...
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bioRxiv - Immunology 2019Quote: ... in combination with cas9 nuclease protein (Merck) was used for gene editing ...
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bioRxiv - Molecular Biology 2021Quote: ... protein lysates were reduced with dithiothreitol (Merck) and cysteine residues were alkylated with iodoacetamide (Merck) ...
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bioRxiv - Plant Biology 2022Quote: ... proteins were transferred on a nitrocellulose (Merck) membrane by wet transfer at 15 V for 6h at 4°C and blocked with 2% skimmed milk at room temperature for 2 hours ...
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bioRxiv - Pathology 2023Quote: ... anti-surfactant protein C (SFTPC, AB3786, Merck), homemade anti-α-smooth muscle actin (clone 1A4 ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were transferred onto PVDF membranes (Merck) and blocked using 5% non-fat dried milk in PBS-Tween 20 ...
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bioRxiv - Cell Biology 2023Quote: ... together with Protein-A Sepharose beads (Merck). Beads were washed 3x in 0.1% (w/v ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentration was measured by the infrared (IR)-based protein quantitation method with a Direct Detect Infrared Spectrometer (Merck). Fifty or thirty-five (depending on the protein detection ...
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bioRxiv - Immunology 2022Quote: ... The protein concentration of ES antigen was estimated using protein estimation kit by Bradford method (Merck GeNei™, Bangalore).
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bioRxiv - Pharmacology and Toxicology 2020Quote: The eluted corona proteins from nanoparticles were first precipitated by using the ProteoExtract® Protein Precipitation Kit (Merck, Germany) as described in the manufacturer protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibody (Merck guinea pig anti-NeuN 1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... A total of 50 μg of protein from each sample was transferred to a protein LoBind plate (Merck, NJ, USA). Protein was reduced with tris (2-carboxyethyl ...