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Citations for Merck :
851 - 900 of 1742 citations for 6 Benzyloxy 1H indole 2 boronic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 ml nuclei wash, and suspension buffer (NWS-Buffer: 2% BSA, 2 mM MgCl2, 0.5% Protector RNAse Inhibitor (SKU3335399001, Merck, Soeborg, Denmark)) was carefully added ...
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bioRxiv - Neuroscience 2023Quote: Place 100 μl trypsin and 100 μl collagenase (per 2 animals) in a 35 mm dish: 2 mg ml-1 Trypsin (Merck - 85450C) and 682 U ml−1 Collagenase in Ca2+ and Mg2+ free medium
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bioRxiv - Plant Biology 2020Quote: ... and the buffer changed to 6 M urea using Amicon ultra-15 centrifugal filter units with 10,000 MWCO (Merck). The protein concentration was determined spectroscopically at 280 nm on a NanoDrop™ spectrophotometer based on the Lambert-Beer’s law assuming a molecular weight of the CBC-Qprot of 47921 Da and an extinction coefficient of 37,400 M−1 cm−1 ...
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bioRxiv - Cell Biology 2021Quote: ... and/or with plasmid DNA (e.g. 1µg/well for 6-well plates) for 24 hrs using GeneJuice (Merck Millipore). siRNAs targeting USP7 ...
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bioRxiv - Cell Biology 2022Quote: ... and were stained on day 6 after the last 1-hour stimulus with dimethyl-methylene blue (DMMB; pH 1.8; Merck) metachromatic dyes as previously described 15 ...
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bioRxiv - Immunology 2022Quote: ... approximately 5×105 Calu-3 cells were pre-treated with kp7-6 (100 ug/mL, CD95/CD95L antagonist, Merck) for 2 hour and then infected with SARS-CoV-2 at an MOI of 0.2 ...
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bioRxiv - Biochemistry 2024Quote: ... Dissolved IntC-UbcH5c-CAET-Ub was transferred to a dialyzer (D-Tube Dialyzer Maxi, MWCO 6– 8 kDa, Merck) in 300 mL of unfolding buffer ...
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bioRxiv - Cell Biology 2023Quote: ... 5 mM K4Fe(CN)6 and 1 mg/ml X-gal (Roth)) and counterstained with nuclear fast red (Merck) for 5 min ...
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bioRxiv - Biochemistry 2023Quote: ... 3 x 105 cells per well were seeded into a 6-well plate in high-glucose Dulbecco’s modified Eagle’s (DMEM) medium (Merck, UK) enriched with 10% FBS (Sigma- Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... plates were washed (6 x 300 µL PBST) and 100 µL of pre-warmed 3,3’,5,5’ tetramethylbenzidine (TMB) solution (Merck-Sigma) was added to each well ...
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bioRxiv - Cell Biology 2024Quote: ... Lentiviruses were added to cells together with 6 µg/ml of polybrene (TR-1003-G, Merck Milipore, Darmstadt, Germany) and incubated for 24 hours.
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bioRxiv - Neuroscience 2024Quote: ... Entry plasmid containing tax-6 coding DNA sequence was amplified with the KOD Hot Start DNA Polymerase (Novagen; Merck). Primers were designed to contain the desired deletion and phosphorylated in 5′ end (File S4) ...
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bioRxiv - Cell Biology 2022Quote: ... The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck, Poznan, Polska). The samples containing 20 µg of protein were mixed with 4× Laemmli loading buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated in low serum medium (0.5% FBS) containing 20 µM retinoic acid (RA) (Sigma-Aldrich; Merck KGaA) for 5 days ...
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bioRxiv - Immunology 2021Quote: ... The digested products were then diluted with formic acid to a final concentration of 0.1% and cleaned up using ZipTip C18 (Merck Millipore). Data was obtained on an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... HPLC-grade ammonium formate (>98%) and formic acid (>98%) used in the LC mobile phase were purchased from Merck (Germany) and Sigma-Aldrich (USA) ...
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bioRxiv - Systems Biology 2020Quote: ... The resultant pellets were resuspended in 3% acetonitrile + 0.1% trifluoroacetic acid and peptide quantification performed using the Direct Detect system (Merck Millipore). Protein samples were normalized then vacuum concentrated in preparation for mass spectrometry.
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bioRxiv - Microbiology 2020Quote: ... the supernatant was then filtered through an acid-washed 0.22 μm polycarbonate filter (GSV, Life Science) using a Sterifil 47 mm filter holder (Merck Millipore). The flow-through liquid was transferred into an acid-washed 10 L polycarbonate (PC ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell pellets were digested with a standard volume of suprapur nitric acid and hydrogen peroxide (Merck Millipore, MA, USA) (2:1 v/v ...
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bioRxiv - Microbiology 2020Quote: ... Samples were stored at 4°C overnight before desilicification with 4% suprapure hydrofluoric acid (Merck; incubation of approximately 5 hours). Afterwards ...
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bioRxiv - Biochemistry 2021Quote: ... Reactions were stopped at each time point using 4 N formic acid and analyzed by PEI-Cellulose-F TLC (Merck) developed in 0.4 M potassium phosphate buffer (pH 3.4) ...
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bioRxiv - Microbiology 2020Quote: ... A was 1 mM ammonium formate pH 9 (for lincomycin detection, prepared by titration of formic acid 98–100%, Merck, Germany with ammonium hydroxide 28–30% ...
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bioRxiv - Microbiology 2022Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 µg/mL 2,6- Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
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bioRxiv - Bioengineering 2022Quote: ... Nitric acid 65%, N,N-dimethylformamide (DMF), sodium hydroxide (NaOH) and Ethylenediamine (EDA, ≥99 %) were purchased from Merck (Darmstadt, Germany). Dulbecco’s modified Eagle’s medium ...
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bioRxiv - Cell Biology 2022Quote: ... 0.05 M cacodylate buffer (using dimethylarsinic acid sodium salt trihydrate from Merck and HCl for pH adjustment to 6.4-7.4) at room temperature in a glass staining block ...
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Insight into the regulatory mechanism of the MFS transporter, SCO4121 by the MarR regulator, SCO4122bioRxiv - Molecular Biology 2024Quote: ... the MSA agar plate was flooded and 500 µg/ml of nalidixic acid (Himedia) and 1 mg/ml of apramycin (Merck). After incubation ...
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bioRxiv - Microbiology 2024Quote: ... Samples were separated on a 1.2 % agarose gel stained with 0.01 % (v/v) GelGreen Nucleid Acid Stain (Merck, Darmstadt, Germany) in TBE buffer for 2 h at 120 V ...
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bioRxiv - Immunology 2024Quote: ... The ethanol and unloaded nucleic acids in the mixtures were removed using Amicon Ultra centrifugal filters (10- or 30-kD MWCO, Merck) for two rounds with 5- and 15-times volumes of 1× PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with 1X PBS and fixed with ice-cold 10% trichloroacetic acid (Sigma Aldrich; Merck cat. no: 27242) at dark ...
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bioRxiv - Systems Biology 2023Quote: ... UK), eosin Y 1% alcoholic and xylene (CellPath, UK), periodic-acid (TCS Biosciences Ltd, UK) and Schiff’s reagent (Merck, Germany). Description of the IF and IHC antibodies used can be found in the Supporting Information.
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bioRxiv - Biophysics 2024Quote: Glass coverslips (Paul Marienfeld GmbH, 24 x 50 mm, 170 ± 5 μm) were overnight incubated in 100 mM Sulphuric acid (Merck). Afterwards the coverslips were rinsed consecutively with Milli-Q water ...
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bioRxiv - Microbiology 2023Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 μg/mL 2,6-Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
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bioRxiv - Neuroscience 2023Quote: ... before transcardially perfusing with 21-28 ml of ice-cold PBS and 4%PFA-0,12% picric acid (Merck, Søborg, Denmark). Spleens were excised and weighed ...
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bioRxiv - Molecular Biology 2023Quote: ... To deplete Xrn1 and Dcp2 with the AID tags (Nishimura et al., 2009; Morawska and Ulrich, 2013) 1-Naphthaleneacetic acid (1-NAA; N0640-25G, Merck) was added to a final concentration of 1 mM ...
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bioRxiv - Neuroscience 2024Quote: ... we cloned the cytoplasmic domain of AChRα (mouse; amino acid 317-428) with or without mCherry into pGEX-4T1 (Cytiva 28-9545-49; Merck). GFP-SH3BP2 with or without the intrinsic disorder domain (amino acid 164-449 ...
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bioRxiv - Immunology 2024Quote: ... for 25 min in the dark at RT followed by a second stage blocking using 1M sulphuric acid (Merck, 1.00731.1000). Optical density (OD ...
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bioRxiv - Plant Biology 2024Quote: ... Protein samples were separated on 12% non-reducing SDS-PAGE gel and cysteine-dimedone protein adducts visualised by western blot analysis using 1:10,000 diluted rabbit anti-cysteine sulfenic acid primary antibody (Merck Millipore) and 1:10,000 diluted horseradish peroxidase conjugated goat anti-rabbit IgG secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... 50 μL of sample (total homogenate or HEPES soluble fraction) was lyophilized for 12 h and digested with 50 µL of Nitric Acid (HNO3) (65% Suprapur, Merck) overnight at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were treated with Picrosirius red (0.1% Direct Red 80 dye in saturated picric acid; Sigma-Aldrich, Merck, Darmstadt, Germany) for 60 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... the media was switched to DMEM high glucose supplemented with 2.5% heat- inactivated FBS and 10 μM Retinoic Acid (Merck, R2625). On day 7 ...
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bioRxiv - Developmental Biology 2024Quote: ... germanica adults using an antisense LNA (locked nucleic acid) probe conjugated to Digoxigenin (DIG) at the 5’ and 3’ ends (5’-DIG-GGAGGTCCCCCAGACCGGCACAGACCGAA-DIG-3’, Merck). Ovaries were dissected under Ringer’s saline ...
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bioRxiv - Cancer Biology 2023Quote: ... DLD-1 cells expressing different EPHB1 wildtype or mutant versions and EFNB1 were mixed in suspension at a ratio of 1:3 and plated at a density of 130,000 cells/cm2 on coverslips coated with 2 mg/cm2 of 1-2 mg/mL laminin (Merck KGaA, Germany, USA) and incubated at 37ºC in 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: Proteins were extracted from 50 hpf zebrafish embryos using the lysis buffer from the calpain 1/2 activity kit (InnoZyme™ Calpain 1/2 Activity Assay Kit CBA054 purchased from Merck Millipore). Protein concentration was adjusted to 2 mg/mL and calpain enzymatic activity was measured in microtubes following the manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: ... and 100 μM 2-mercaptoethanol (Sigma-Aldrich; Merck KGaA) (DMEM+) ...
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bioRxiv - Developmental Biology 2021Quote: ... the slides were stained with 2% Giemsa (Merck KGaA) for 8 min ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 2% ß-Mercaptoethanol and 500 units/ml Lyticase (MERCK)) and incubated at 37°C for 2 h ...
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bioRxiv - Molecular Biology 2020Quote: ... buffer containing 2 mM MgCl2 (Merck Millipore, MA, USA) with 10 μM AMP (Sigma 01930 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then incubated in 2 M HCl (Merck) with 0.5% Triton X-100 (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... 2 mg/ml of fast green (Merck-Sigma-Aldrich) was added to the heparin solution to verify effectiveness of the injection ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 2% foetal bovine serum (FBS, Merck, Germany), pen/strep and amphotericin B.