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Citations for Merck :
801 - 850 of 1322 citations for Recombinant Human FGF23 Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... pSIH-mCherry-KDEL construct expressing lentiviruses were added to the suspension of motoneurons before plating on polyornithine-(PORN) and human merosin-coated (CC085, Merck-Millipore) plates ...
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bioRxiv - Microbiology 2021Quote: ... Cytokine quantification was performed using a Human Cytokine/Chemokine/Growth Factor Panel A 48-Plex Premixed Magnetic Bead Multiplex Assay (Merck Millipore), using the Luminex MAGPIX System in 96-well plate format ...
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bioRxiv - Neuroscience 2022Quote: Human Neural Stem Cells (hNSCs) derived from the NIH approved H9 (WA09) human embryonic stem cell line were purchased from Merck (Germany). The hNSCs were plated on matrigel-coated 100 mm petri dishes and maintained in culture with NSCs expansion media (KO DMEM/F12 ...
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bioRxiv - Cell Biology 2022Quote: ... human very-low-density and low-density lipoproteins (catalog no. 437647-5MG and 6 mg/ml, LP2-2MG, Merck Millipore, respectively) were purchased and stored according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2023Quote: ... and PBMCs (1 × 106 cells per well) were seeded in 96-well plates in RPMI supplemented with 1% PNS and 10% AB human serum (Merck, UK) and stimulated with SARS-CoV-2 specific peptides pools ...
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bioRxiv - Developmental Biology 2023Quote: ... a micro-infusion pump (iPRECIO SMP-200, ALZET Osmotic Pumps) with 15 IU/kg human follicle-stimulating hormone (hFSH, Gonal-f; Merck Biopharma) was embedded subcutaneously under anaesthesia and injected 7 µL/h for 10 days ...
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bioRxiv - Neuroscience 2024Quote: ... MACS-enriched from fresh PBMC were seeded at 0.5×106 cells/cm2 in RPMI plus 5% heat-inactivated human AB serum (Merck Life Sciences) and 100 U/ml penicillin-streptomycin and cultured with 50 ng/ml rhIL-4 (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: CM was collected from TIS MDA-MB-231 cells for chemokine and cytokine analyses using a Milliplex MAP human cytokine/chemokine magnetic bead panel (Merck Millipore) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... Primary human T-cells were isolated from peripheral blood (NHS Blood & Transplant Service, UK) using Ficoll-PaqueTM (Merck Group, Darmstadt, Germany) density gradient centrifugation followed by positive selection using antibody-coated magnetic beads directed against either human CD4 or CD8 antigen (Miltenyi Biotechnology ...
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bioRxiv - Developmental Biology 2021Quote: ... males were boosted with 100 units (U) of human chorionic gonadotropin (HCG: Chorulon®, Merck, NADA NO.140-927, Code No. 133754) and females were primed with 10U HCG ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... rat slices were exposed to 3 μM dihydroethidium (DHE) while in humans’ sections it was added 4 μM DHE (Merck, Darmstadt, Germany) for 30 minutes at 37ºC 51 ...
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bioRxiv - Biochemistry 2023Quote: Human osteosarcoma SJSA-1 cells were purchased from ATCC (Manassas, VA, USA) and cultured with RPMI 1640 medium (Sigma Aldrich, Merck, Germany), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2020Quote: ... Total protein concentration in eluates was measured using Direct Detect infrared spectroscopy (Merck, Darmstadt, Germany).
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bioRxiv - Cell Biology 2019Quote: ... Proteins were concentrated with Amicon Ultra-15 Ultracel 10k centrifugal filter units (Merck Millipore, #UFC901024). Purification of cytoplasmic actin ...
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bioRxiv - Pathology 2022Quote: ... XO Activity was expressed as units/mg protein using native XO from buttermilk (Merck Millipore) as standard.
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bioRxiv - Molecular Biology 2021Quote: ... Protein-G fast flow bead resin and Immobilon-P PVDF membrane were purchased from Merck-Millipore (USA) ...
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bioRxiv - Molecular Biology 2020Quote: ... the separated proteins were transferred onto a polyvinylidene fluoride membrane (Immobilon R, Merck Millipore Ltd) for 1 hour at 20 V ...
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bioRxiv - Biophysics 2019Quote: ... the protein sample was filtered using a 30 kDa centricon filter (Merck Millipore, Darmstadt, Germany). The crude protein was then injected into a DEAE anion exchange column equilibrated with 10 mM Tris (pH 7.4 ...
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bioRxiv - Molecular Biology 2019Quote: ... Total protein of EECs was extracted with 1X RIPA buffer (20–188, Merck, Darmstadt, Germany), supplemented with Halt™ Proteinase & Phosphatase Single-Use inhibitor cocktail (78442 ...
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bioRxiv - Immunology 2020Quote: ... the Protein G chip was regenerated for 30 seconds with 100 mM Glycin-HCl (Merck) pH 1.5 at 30 µL/min and the process was repeated starting a new cycle with the capture of the potentiating antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Equal loading of protein samples on the membrane was confirmed using Ponceau S (MERCK, P3504) staining (2% Ponceau S (w/v ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated to 12 mg/mL using an Amicon Ultra Centrifugal Filter (Merck-Millipore). To purify non-degraded PrgA (without the S476A mutation) ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were transferred to a 0.45 μm pore size polyvinylidene fluoride transfer membrane (Merck Millipore), and nonspecific binding was blocked in Tris-buffered saline/0.1% Tween 20 with 5% skim milk powder ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were concentrated using Ultra-15 mL centrifugal filter devices (Amicon, Merck KGaA, Darmstadt, Germany) with a molecular weight cut-off (MWCO ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were transferred from the polyacrylamide gel to a PVDF membrane (Merck Millipore, Massachusetts, USA). Membranes were blocked with Rockland blocking buffer (Biomol GmbH ...
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bioRxiv - Cell Biology 2021Quote: ... Intracellularly applied drug: the membrane impermeable G-protein blocker GDP-β-S (2 mM, Merck) (Farkas et al. ...
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bioRxiv - Biophysics 2020Quote: ... Eluted protein was concentrated to ∼ 500uL using Amicon Ultra 5 centrifugal filters (Merck, Darmstadt, Germany). The affinity purified protein was further separated by gel filtration using a Phenomenex Yarra 3µm SEC-4000 300 x 7.8mm column (Phenomenex ...
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bioRxiv - Biochemistry 2022Quote: ... Purified protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 50 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... Purified protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 50 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... 10 µM Cdc5Plk1 re-phosphorylated Mif2 protein (M3) was immobilized on anti-FlagM2 beads (Merck) and incubated with 25 µM Ame1/Okp1 complex in binding buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... The proteins were concentrated to 50−100 µM using the Amicon Ultra centrifugal filters (Merck). For protein labelling ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted protein solution was applied to a MonoQ 10/100 column (Sigma-Aldrich, Merck), equilibrated in MonoQ buffer A (40 mM Hepes ...
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bioRxiv - Developmental Biology 2022Quote: ... the protein extract was loaded onto the centrifugal filter CO10 kDa (Merck Millipore, Darmstadt, Germany), and detergent were removed by washing five times with 8M Urea (Merck ...
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bioRxiv - Developmental Biology 2022Quote: ... The eluted proteins were then purified by Fractogel® EMD TMAE (Merck KGaA, Darmstadt, Germany) or SP-Sepharose (GE-Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... and then the proteins were transferred onto polyvinylidene difluoride membranes (Merck Millipore, Billerica, MA, USA).
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bioRxiv - Cell Biology 2022Quote: All proteins used in this study were overexpressed in Rosetta (DE3, pLysS) Escherichia coli (MERCK) cells using 0.4 mM isopropyl 1-thio-D-galactopyranoside (Inalco Pharmaceuticals ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins in the gel were then transferred to a poly-1,1-difluoroethane (PVDF) membrane (Merck) in transfer buffer (25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... proteins separated by SDS-PAGE were transferred to a PVDF membrane (Immobilon-P, Merck Millipore) in a Mini Trans-Blot (Bio-Rad ...
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bioRxiv - Immunology 2019Quote: Protein lysates from cells were obtained by lysis in radioimmunoprecipitation assay buffer (Merck Millipore, #R0278) with protease and phosphatase inhibitors (Roche ...
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bioRxiv - Microbiology 2019Quote: Protein samples were separated by SDS-PAGE and blotted on PVDF (polyvinylidenfluoride) membranes (Merck Millipore). Membranes were blocked for 1h with PBS ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were transferred into methanol pre-activated polyvinylidene fluoride (PVDF) membranes (Immobilon-P, Merck-Millipore). After transference ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was then concentrated to 1 mg/ml using Amicon device 30K (Merck Millipore) and stored at -80°C.
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bioRxiv - Microbiology 2020Quote: ... The purified protein was desalted and concentrated with the Centricon cutoff 10 kDa (Merck Millipore). The purity of the protein was analyzed by SDS-page and Western-blot using Anti-His antibodies.
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bioRxiv - Microbiology 2021Quote: ... MS2 capsid proteins were detected with a polyclonal rabbit antibody (ABE76-I, Merck Millipore, France).
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bioRxiv - Synthetic Biology 2020Quote: ... eluted protein fractions were concentrated using Amicon® Ultra-15 centrifugal filter units (Merck, USA) with a 100 KDa cut-off ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was then concentrated to 1 mg/ml using Amicon device 100K (Merck Millipore) and stored at -80°C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and the purified proteins were concentrated with Amicon Ultra-0.5 Centrifugal Filter devices (Merck Millipore) following manufactures instructions ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was concentrated using a 30 kDa MWCO centrifugal filters (Amicon Ultra; Merck Millipore) and then gel filtered ...
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bioRxiv - Neuroscience 2021Quote: ... as confirmed by the anti-glial fibrillary acid protein (anti-GFAP) antibody (Merck, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2022Quote: RIP was performed according to the Magna-RIPTM RNA-binding protein immunoprecipitation kit (Millipore/Merck) as previously described (Papoutsoglou et al ...