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Citations for Merck :
801 - 850 of 1388 citations for Chikungunya Virus E2 Envelope Protein HEK293 Human Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... A pre-clear step was performed by adding protein A-agarose beads (Merck Millipore) to the lysates ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was concentrated by Amicon Ultra-15 Centrifugal Filters (10 KDa) (UFC901008, Merck) and diluted in buffer HT with 50 mM KCl for decreasing of KCl concentration to 90 mM ...
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Molecular Insights into the Recognition of Acetylated Histone Modifications by the BRPF2 BromodomainbioRxiv - Biochemistry 2022Quote: ... Purified proteins were concentrated using Amicon Ultra-10k centrifugal filter device (Merck Millipore Ltd.), and the concentration was determined using Bradford assay kit (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluted proteins were concentrated by Amicon Ultra Centrifugal Filter 50 kDa (Merck Millipore) and purified on a Superose 6 10/300 GL column (GE Healthcare ...
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bioRxiv - Biophysics 2022Quote: ... eluted protein was concentrated to 500 μl via Amicon Ultra 5 centrifugal filters (Merck) and separated by Phenomenex Yarra 3 μm SEC-4000 300 × 7.8 mm column (Phenomenex ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were resolved in SDS-polyacrylamide gels and blotted onto PVDF membranes (Millipore/Merck) or nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... special AT-rich sequence binding protein 2 (SATB2; clone EP281, Merck, USA; 1:200), and programmed cell death ligand 1 (PD-L1 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were further concentrated to 2-10 mg/ml using Amicon concentration devices (Merck, 3,000 Da ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μg purified recombinant LARK protein or 1 μg BG4 (MABE917, Merck, Darmstadt, Germany) was added ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Immunology 2022Quote: ... The eluted protein was then concentrated with a 100KD Amicon® Ultra concentrator (Merck) and washed with PBS before quantification using a NanoDrop™ ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... for each IP reaction 50 μl of Protein A agarose suspension (Merck Millipore, IP02) was incubated with 1.5 μg of GFP antibody (mouse anti-GFP ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were resolved by and blotted on Transfer membrane (Immobilon-P, Merck Millipore IPVH00010). Membrane blocking (5% BSA/TBS ...
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bioRxiv - Molecular Biology 2023Quote: The trypsinized protein samples were subjected to Iodacetamide (IAA, 5 mg/ml, Merck, 8.04744.0025) and StageTip purified (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... anti-GFP antibodies and 20 µl of protein G–Sepharose (Merck, GE17-0618-01). The sepharose beads were washed twice with lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... was subjected to affinity purification using S-protein-agarose (Merck Millipore, Burlington, MA, USA) for one to two hours and washed four times ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was filtered through a 0.45μm ultra-low protein binding filter (Merck Millipore). Aliquots were stored at −80 °C.
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bioRxiv - Biophysics 2023Quote: ... Resolved proteins were then electrophoretically transferred onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, USA) at 90 V for 2.5 h ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was concentrated with an Amicon Ultra-4 (Merck Millipore, MWCO 3 K) to a concentration of 726 µM ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were diluted with 90% methanol/100 mM triethylammonium bicarbonate (TEAB) (Merck Darmstadt, Germany) for efficient trapping in Micro S-Trap columns (ProtiFI ...
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bioRxiv - Physiology 2023Quote: Proteins were extracted from powder on ice using lysis buffer [150 mM NaCl (Merck), 10 mM Tris/HCl (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein was then transferred to 0.4 μm polyvinylidene fluoride (PVDF, Merck Millipore) membrane ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred onto a PVDF membrane (Immobilon-P, Merck Millipore, Massachusetts, USA) and the membranes were blocked with PBS containing 5% non-fat dry milk for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... Protein expression was performed in the presence of 3.5 mM valproic acid (Merck Millipore) and 10 μM kifunensine (Biomol) ...
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bioRxiv - Developmental Biology 2023Quote: ... NuPage 4 to 12% bis-tris protein gels (NP0323, Thermo Scientific; or MP42G15; Merck) before transferring into 0.22μm pore size polyvinylidene difluoride (PVDF ...
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bioRxiv - Plant Biology 2023Quote: ... Purified proteins were concentrated using 10 kDa ultra-centrifugal filter Amicon (Merck Millipore, Germany). The quality and quantity of proteins were analyzed by SDS-protein gel electrophoresis (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentration was adjusted by concentrating of the samples on 100kDa filters (Amicon, Merck). Samples were loaded on 10% SDS-PAGE gel and SPATEs were visualized by Coomassie Blue staining (Instant Blue ...
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bioRxiv - Cell Biology 2023Quote: ... the protein was concentrated using a 50 kDa cut-off Amicon filter (Merck Millipore) and loaded onto a pre-equilibrated Superdex 200 Increase 10/300 GL column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: ... the protein was concentrated using a 50 kDa cut-off Amicon filter (Merck Millipore) and loaded onto a pre-equilibrated Superdex 200 Increase 10/300 GL column (Cytiva) ...
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bioRxiv - Biophysics 2023Quote: ... we blotted the proteins at RT on a PVDF membrane (Merck, Cat. No.: IPVH00010). We blocked the membranes for 1 h at RT with sterile filtered 5 % bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2023Quote: ... SDS-PAGE and protein transfer to polyvinylidene difluoride (PVDF) membrane (Immobilon-P, Merck Millipore) was performed with the NuPAGE Novex and XCell II Blot Module systems (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were concentrated by ultrafiltration on 10K Amicon® Ultra Centrifugal Filters (Merck Millipore), aliquoted ...
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bioRxiv - Genetics 2024Quote: ... Then the resolved proteins were transferred to 0.45 μm PVDF membranes (Merck Millipore, IPVH00010). The membranes were blocked ...
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bioRxiv - Biophysics 2024Quote: ... The dialyzed protein solution was filtered through a 0.22 μm membrane (Merck Millipore, SLGP033RB) and injected onto an ion-exchange Q column (GE Healthcare ...
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bioRxiv - Plant Biology 2024Quote: ... The separated proteins were transferred to Immobilon®-P PVDF membrane (Merck Millipore, USA) and probed with primary antibodies overnight ...
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bioRxiv - Neuroscience 2024Quote: ... For protein reduction gel cubes were mixed with freshly prepared 10mM DTT (dithiothreitol; Merck) in 50mM BA and incubated 1 h at 56C ...
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bioRxiv - Microbiology 2024Quote: ... Purified protein samples were concentrated using Amicon 10,000 MWCO centrifugal filter units (Merck, Germany) and stored on ice at 4°C until use ...
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bioRxiv - Microbiology 2024Quote: ... Purified proteins were concentrated using 10 kDa cutoff Amicon Ultra Centrifugal Filters (Merck Millipore) and stored in 50% v/v glycerol at -20 °C until further use.
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bioRxiv - Biochemistry 2023Quote: ... The protein was concentrated using Amicon Ultra-0.5 10K Centrifugal Filter Devices (Merck Millipore). The protein concentration was measured on a Nanodrop 1000 spectrophotometer at 280 nm using the extinction coefficient of Trp and Tyr residues in the GST-LC3B sequence (48820 cm-1M-1).
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bioRxiv - Pathology 2024Quote: ... the resolved protein samples were transferred onto a PVDF membrane (Merck Millipore Ltd., USA) through a Bio-Rad electrophoretic blotting apparatus ...
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bioRxiv - Biochemistry 2020Quote: ... cells were stimulated for the indicated periods of time with antibodies to human CD3 (7.5 μg/mL, Cat. number 217570, Merck; Jurkat cells) and EGF (50 ng/mL ...
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bioRxiv - Physiology 2019Quote: ... following which they were treated with media containing 20% human serum (fed or fasted) and 1 µM puromycin (Merck Millipore Limited) for a further 4 h ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... PAb directed against phospho-STAT2 (Tyr689) and MAb directed against human IFNα/β receptor chain 2 (MAB1155) were purchased from Merck-Millipore.
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bioRxiv - Biochemistry 2021Quote: ... These Wild Type or Mutant Type let-7a-5p plasmids were co□transfected into treated human chondrocytes cells along with NC mimics or KCNQ1OT1 mimic (Sigma□Aldrich; Merck KGaA) using Lipofectamine 6000 following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and the Human Cytokine/Chemokine Magnetic Bead Panel (Cat# HCYTMAG-60K-PX30) on a Luminex MAGPIX system (Merck Millipore, Billerica, MA). Conditioned media concentrations (pg/mL ...
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bioRxiv - Microbiology 2022Quote: ... Aliquots of them were analyzed with the MILLIPLEX human cytokine/chemokine magnetic bead panel (HCYTOMAG-60K, Merck Millipore, Burlington, MA, USA) using the Luminex 200 multiplexing instruments (40-012 ...
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bioRxiv - Molecular Biology 2020Quote: ... mt1 and mt2 were generated by inserting the ORF of human TruB1 and its mutants into the pET22b vector (Merck Millipore) at the NdeI and XhoI sites ...
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bioRxiv - Neuroscience 2021Quote: ... pSIH-mCherry-KDEL construct expressing lentiviruses were added to the suspension of motoneurons before plating on polyornithine-(PORN) and human merosin-coated (CC085, Merck-Millipore) plates ...
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bioRxiv - Microbiology 2021Quote: ... Cytokine quantification was performed using a Human Cytokine/Chemokine/Growth Factor Panel A 48-Plex Premixed Magnetic Bead Multiplex Assay (Merck Millipore), using the Luminex MAGPIX System in 96-well plate format ...
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bioRxiv - Neuroscience 2022Quote: Human Neural Stem Cells (hNSCs) derived from the NIH approved H9 (WA09) human embryonic stem cell line were purchased from Merck (Germany). The hNSCs were plated on matrigel-coated 100 mm petri dishes and maintained in culture with NSCs expansion media (KO DMEM/F12 ...