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Citations for Merck :
801 - 850 of 872 citations for 5 METHYLPICENE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... were randomly assigned to receive an injection of 0.9% NaCl v/v 5-bromo-2’Deoxyuridine (BrdU) 100 mg/kg (MERCK; New York, USA) three times within the same day ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μl of sample was injected in 50 % B and the analysis was performed using the gradient of 15 % A and 85 % B for 3.5 min followed by an increase to 75 % A and 25 % B over 11.5 min and its continuation for 10 min with the SeQuant® ZIC®-pHILIC column (5 µm, 150 mm x 2.1 mm, Merck, USA), at a flow rate of 0.13 ml/min.
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bioRxiv - Microbiology 2020Quote: ... water was purified by a Milli-Q system (resistivity 18.2 MΩ cm at 25°C, TOC < 5 ppb, Merck Millipore, Molsheim, France). Indole-3-acetic-2,2-d2 acid (≥ 98% ...
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bioRxiv - Neuroscience 2020Quote: ... half of the medium was replaced with fresh medium containing 20 μM of 5-fluorodeoxyuridine and uridine (Sigma Aldrich, Merck, Darmstadt, Germany) every 3 days ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The protein was concentrated overnight to a volume of 2 mL in a Vivaspin 20 Ultrafiltration Unit (5 kDa MWCO)(Merck, Darmstadt, Germany) and then loaded onto a HiLoadTM 26/60 Superdex TM 75 prep grade (GE Healthcare ...
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Microbiology 2021Quote: ... protease cleavage site at the N-terminus site was subcloned between KpnI (5’-terminus) and HindIII (3’-terminus) restriction enzyme sites in the pRSF-1b vector (Merck, Darmstadt, Germany) to express His-TEV protease site-MA protein (pRSF-1b_MA ...
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bioRxiv - Molecular Biology 2021Quote: ... mESCs were grown on gelatin-coated plates under standard culture conditions (37°C, 5% CO2, humid) atop a ‘feeder’ layer of MitomycinC-inactivated (Merck Life Science) SNLP mouse fibroblasts and passaged upon ∼80% confluency every 2-3 days using TrypLE Express (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... Water was purified by a Milli-Q system (resistivity 18.2 MΩ cm at 25°C, TOC < 5 ppb, Merck Millipore, Molsheim, France). For laboratory culture samples ...
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bioRxiv - Cell Biology 2022Quote: ... protease inhibitor cocktail [5 μl/ml] and phosphatase inhibitor cocktail [10 μl/ml]) and 0.5% Nonidet P-40) (I8896; Sigma-Aldrich/Merck group, USA). After 10 min on ice ...
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bioRxiv - Cell Biology 2022Quote: ... Cell supernatant (50 mL) was then concentrated to 5 mL by centrifugation using Amicon® Ultra-15 Ultrafiltration Centrifuge Tubes (Millipore; Merck KGaA) following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... were left to soak for no less than 24 hours in either plain water (for the cryptic/plain treatment) or a 2.5% Bitrex™ aqueous solution (5 g denatonium benzoate (Merck, Darmstadt, Germany) dissolved into 200 ml water ...
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bioRxiv - Microbiology 2022Quote: ... solution containing 16 mM NaHCO3 and 0.15 mM ZnSO4 as well as on 5 mg/ml imipenem – 5 mg/ml cilastatin in 0.15 mM ZnSO4 prepared from the brand name Primaxin formulation (Merck, Sharp& Dohme Corp.) were also examined.
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bioRxiv - Microbiology 2022Quote: ... 0.5 ml of each aliquoted sample was filtered onto a 25 mm polycarbonate filter (pore size: 0.45 µm, Merck KGaA, Darmstadt, Germany). The filters were then air dried under sterile conditions and mounted onto 25mm diameter aluminum pin stubs (Agar Scientific Ltd. ...
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bioRxiv - Microbiology 2022Quote: ... the medium was replaced with complete medium without phenol red and 10 μL of 5 mg/mL MTT solution (In vitro toxicology assay kit, MTT-based, TOX-1KT, Merck, Darmstadt, Germany) were added to each well ...
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bioRxiv - Microbiology 2022Quote: ... Frozen larvae were thawed for two min in a solution of 50:50 5% bleach (Danklorix, CP GABA, Hamburg, Germany) and Milli-Q (Merck, Darmstadt, Germany) water for surface sterilization (Amiri et al. ...
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bioRxiv - Microbiology 2023Quote: ... 30 mL buffy coat was diluted with 5 mL DPBS (BioWhittaker®) and carefully poured over 15 mL of Histopaque® (Merck). After centrifugation (500 g ...
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bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
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bioRxiv - Plant Biology 2023Quote: ... The combined aqueous phase gave a total volume of 450 mL of which 5 uL were measured by Method 1 and compared against freshly prepared authentic standards (Merck, Darmstadt, Germany). We wish to note that our acid quantification generated much higher absolute values than an earlier study (Szecowka et al ...
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bioRxiv - Cell Biology 2023Quote: ... Protein transfer was then visualized using 0.1 % Ponceau S solution (w/v) in 5% acetic acid (Merck Life Sciences, Cat #: P7170-1L) and imaged ...
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bioRxiv - Evolutionary Biology 2023Quote: ... A small aliquot of each sample was added to Hemi medium17 with a 5-μl/ml antibiotic cocktail (P4083, Merck, Darmstadt, Germany) and incubated under dark conditions at 19–20 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: CC strips were dissected and then transferred to microtiter plate wells containing 5 μM bis-N-methylacridinium nitrate (Lucigenin; Merck, Darmstadt, Germany), some strips were stimulated with NADPH (100 μM ...
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bioRxiv - Bioengineering 2023Quote: ... two thirds of the medium was removed from all wells and T cells were treated with 5 ng/mL polybrene (Merck, Darmstadt, Germany). Lentiviral particles (MOI=3 ...
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bioRxiv - Biochemistry 2023Quote: ... dehydrated with 100 μL acetonitrile and rehydrated with 25 μL trypsin solution, containing 5 ng/μL trypsin (cat # 37283, SERVA Electrophoresis GmbH, Heidelberg) in 3 mmol/L ammoniumbicarbonate (cat # 09830, Merck KGaA, Darmstadt). After 4 h incubation at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The blots were developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-brom-4-chlor-3-indoxylphosphate (BCIP; Merck, Darmstadt, Germany) for 5–30 min at RT ...
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bioRxiv - Cancer Biology 2023Quote: A Thermo Ultimate 3000 high performance liquid chromatography (HPLC) system was equipped with a ZIC-pHILIC column (SeQuant; 150 mm by 2.1 mm, 5 μm; Merck KGaA, Darmstadt, Germany), with a ZIC-pHILIC guard column (SeQuant ...
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bioRxiv - Cell Biology 2023Quote: ... fixed cells were pelleted (1500 RPM 5 min) and resuspended in PBS containing 20 µg/ml propium iodide and 200 µg/ml RNase (Merck Life science).
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bioRxiv - Plant Biology 2024Quote: ... cinerea spores from glycerol stocks were diluted to a concentration of 5 x 104 spores/ml in ¼ PDB (Merck KGaA, Darmstadt, Germany) and pre-germinated for 4 hours at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... Conditioned medium was then centrifuged at 1500 rpm for 5 min and concentrated 30x times in Amicon® Ultra-4 Centrifugal Filter Unit with 30 kDa MWCO cut-off (Merck Millipore). Concentrated conditioned medium was then filtered using 0.2 μm filters and frozen until use.
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bioRxiv - Molecular Biology 2024Quote: ... fixed with 2% paraformaldehyde (PFA) in PHEM and washed once for 5 min at (RT) with 3% BSA (bovine serum albumin, Merck-Sigma, Germany) in Tris-buffered saline with 10 mM EGTA and 2 mM MgCl2 (TBSTEM) ...
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bioRxiv - Neuroscience 2024Quote: ... Postoperative care included antibiotic and analgesic administration (2.27% enrofloxacin, 5 mg/kg, Bayer, Kiel, Germany and 0.3 mg/ml buprenorphine, 8 µg/kg, Buprex, Merck & Co., Inc., NJ, USA) in a controlled temperature environment (37°C) ...
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bioRxiv - Microbiology 2024Quote: ... one stationary liquid culture was poured into a 50 mL syringe and plunged through a sterile 5 μm membrane filter (Millex-SV, Merck, Darmstadt, Germany), with slow and consistent pressure ...
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bioRxiv - Cell Biology 2020Quote: ... Beads were then washed 2x in Tween wash buffer (5 mM Tris-HCl [Sigma], 0.5 mM EDTA [AppliChem], 1 M NaCl [Merck], 0.05 % Tween-20 [Sigma]) and 1x in H2O ...
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bioRxiv - Microbiology 2019Quote: ... Chlorophyll a content was determined using a microplate reader (BMG Polarstar Optima) after filtration of the sub-samples (5 to 10 mL) through Whatman GF/F glass circles (25 mm) (Merck KGaA, Darmstadt, Germany) and 90% acetone extraction of the filtrate for 24 h in the dark at 4 °C [74].
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bioRxiv - Biochemistry 2020Quote: ... Total extract was clarified by 20 min centrifugation at 30,000 rcf at 4°C and the soluble fraction was loaded on affinity chromatography over 5 mL of NiNTA resin (Sigma-Aldrich Merck, Darmstad Germany). Resin was step-washed with 10 mL of buffer A supplemented with 10 ...
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bioRxiv - Biophysics 2020Quote: ... functionalized with either 100 μg/mL poly-D-lysine overnight for microglia cells or with 0.2 mg/mL fibronectin (FC010, Merck, 1:5 in PBS) for 2h at 37°C for fibroblasts ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were immersed in Neo-Clear® three times for 5 minutes and then mounted using Neo-mount® mounting medium (Merck Millipore), coverslipped and left to dry overnight in at 42°C.
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in blocking solution (5% bovine serum albumin in PBST) for 60 min at room temperature and then incubated with anti-CB1R (Merck Millipore, 1:1000) in blocking solution overnight at 4 °C ...
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bioRxiv - Bioengineering 2019Quote: ... The supernatant was passed through the cartridges (pre-conditioned with 5 mL each of tert-methyl butyl ether, methanol (Merck; laboratory grade purity) and deionised water ...
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bioRxiv - Genomics 2022Quote: ... followed by quenching with final 1 x Glycine solution for 5 min at RT utilizing the Magna Nuclear RIP (Cross-Linked) Nuclear RNA-Binding Protein Immunoprecipitation Kit (Merck millipore, 17-10520). Cross-linked cells were then centrifuged at 800 x g at 4°C and washed three times with ice cold DPBS ...
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bioRxiv - Microbiology 2022Quote: ... mice were gavaged with 0.2 ml of Fluorescein Isothiocyanate-Dextran (FITC-Dextran; 3–5 kDa; cat. no. FD4; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) in PBS ...
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bioRxiv - Microbiology 2022Quote: ... After 72 hours of incubation at 33°C 5% CO2 wells were stained with Gram’s crystal violet solution (Merck KGaA, 64271 Damstadt, Germany) plus 5% formaldehyde 40% m/v (Carlo ErbaSpA ...
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bioRxiv - Systems Biology 2022Quote: ... Chromatographic separation was performed on either of the three columns: a ZIC-pHILIC column (Merck, 150 mm × 2.1 mm, 5 μm particle size), a reverse-phase C18 Kinetex Evo column (Phenomenex ...
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bioRxiv - Immunology 2023Quote: ... After 72 hours of incubation at 33°C 5% CO2 wells were stained with Gram’s crystal violet solution (Merck KGaA, 64271 Damstadt, Germany) plus 5% formaldehyde 40% m/v (Carlo ErbaSpA ...
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bioRxiv - Cancer Biology 2023Quote: ... peptides were prepared by lysis of cells with a 5% SDS solution and Roche cOmplete™ Mini EDTA-free Protease Inhibitor Cocktail (Merck Darmstadt, Germany), followed by sonication (10 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... The flow through and wash fractions were concentrated to 3-5 mg/mL using Amicon ultrafiltrators (10 kDa MWCO, Merck Millipore, Darmstadt, Germany). All fractions were combined and dialyzed 2x over night at 4 °C against 5 L 10 % (v/v ...
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bioRxiv - Physiology 2023Quote: ... Pigment separation was performed by reverse-phase HPLC as described in (Färber et al., 1997) using a Dionex BioLC HPLC system (Sunnyvale, California, US) with LiChrospher® 100 RP-18 (5 µm) column (Merck, Darmstadt, Germany). Xanthophylls ...
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bioRxiv - Genetics 2022Quote: ... 5 µg SA8 was dissolved in 10 µL 10 mM hydrochloric acid and digested with 20 ng/µL pepsin (Merck, cat. No. 10108057001) at 37 °C for 6 h ...
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bioRxiv - Molecular Biology 2022Quote: ... was placed on the top of a water-cooled glass column (33 × 2.5 cm) filled with a slurry of silica gel 60 (with the addition of 7 % water, 40 – 63 μm, Merck, Darmstadt, Germany, # 1.09385.2500) and n-pentane ...
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bioRxiv - Molecular Biology 2023Quote: ... Milli-Q water was used for preparing all aqueous solutions (18 MΩ×cm, <5 ppb, Millipore Milli-Q® Reference A+ system, Merck Millipore, Germany). LC-MS grade acetonitrile (ACN ...