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Citations for Merck :
801 - 850 of 1249 citations for 3 Trifluoromethylthio benzeneboronic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Tissues were homogenised by gently resuspending 3-5x in 200 μl Accumax solution (Merck, A7089), followed by a 15 min incubation at 37°C on a shaker (57 RPM ...
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bioRxiv - Microbiology 2024Quote: ... Active human recombinant caspase-3 (#CC119) and caspase-9 (#CC120) proteins were purchased from Merck.
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bioRxiv - Microbiology 2024Quote: ... polyethylene glycol was added to a final concentration of 5% (Cat # 25322-68-3, Merck), and a LFA strip (Cat # 3822-9000 ...
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bioRxiv - Biophysics 2024Quote: ... The fractions containing GRB2 SH2 domain were concentrated by Amicon Ultra-15 3 kDa (Merck) and then incubated with uTEV3 protease to remove the GST tag in buffer D for approximately 16 h at 25 °C ...
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bioRxiv - Plant Biology 2024Quote: ... at a dilution of 1:1000 in 3% BSA and 0.5% Triton X-100 (Merck) overnight (o/n) ...
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bioRxiv - Biophysics 2024Quote: ... 1,2-Distearoyl-sn-glycero-3-phosphocholine (DSPC, CAS: 816-94-4) was sourced from Merck KGaA (Darmstadt ...
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bioRxiv - Biochemistry 2024Quote: ... buffer exchanged into buffer A and concentrated using 3 kDa MW-CO Amicon (Merck-Millipore).
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bioRxiv - Cancer Biology 2024Quote: ... 3T3-J2 fibroblasts were treated for 3 hours with 4 μg/mL mitomycin C (Merck) at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Endogenous peroxidase activity was blocked by incubating slides in 3% (v/v) HO (Merck H1009). Endogenous avidin and biotin were then blocked with the Abcam Avidin/Biotin blocking kit (Abcam ab64212) ...
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bioRxiv - Biophysics 2022Quote: ... three washes in PBS+10%HS each lasting 5min and staining of nucleic acids with Hoechst 33342 (5μg/mL-Merck Bio-sciences) for 5min ...
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bioRxiv - Cell Biology 2022Quote: ... the zona pellucida was removed from metaphase I oocytes or metaphase II-arrested eggs by washing cells through Tyrode’s acid solution (Merck, T1788-100ML) droplets covered with mineral oil (52) ...
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bioRxiv - Plant Biology 2020Quote: ... tumefaciens were resuspended in 50 mM MES (2-Morpholinoethanesulfonic acid hydrate) (Duchefa, Haarlem, The Netherlands) - KOH buffer (pH 5.6) containing 2mM NaH2PO4 (Merck, Darmstadt, Germany), 100 µM acetosyringone (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then resuspended in 10 μl of 0.1% trifluoroacetic acid and tryptic peptides were desalted using a Zip-Tip® column (Merck Millipore) pipette tip according to the manufacturer’s protocol and dried in a vacuum concentrator before analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
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bioRxiv - Biochemistry 2023Quote: ... and mouse BRCA2 (amino acid residues: 2232-2283) were cloned into pMAT11 (Peranen et al., 1996) and pRSF-Duet1 (Merck Millipore) vectors for expression with an N-terminal TEV-cleavable His6-MBP tag and an N-terminal TEV-cleavable MBP tag ...
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bioRxiv - Cell Biology 2023Quote: ... produced by the mix of 50 ml of sodium hypochlorite 10% (Roth, ref. 9062.3) and 2 ml of 37% hydrochloric acid (Merck, ref. 1.00317.1000). Seeds were then aerated for 10 min in a sterile bench to remove the left-over chlorine gas ...
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bioRxiv - Biochemistry 2023Quote: ... Constructs of mouse BRME1 (amino acid residues: 540-578 and 540-574) were cloned into pMAT11 (Peranen et al., 1996) and pRSF-Duet1 (Merck Millipore) vectors for expression with an N-terminal TEV-cleavable His6 tag ...
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bioRxiv - Cancer Biology 2024Quote: ... was followed by 5 minutes of rinsing with water and 20-30 seconds of Eosin solution (Merck, 115935, 100ml supplemented with one drop of acetic acid, Merck, 1.00063). For PAS staining ...
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bioRxiv - Systems Biology 2024Quote: ... The solvent system comprised solvent A consisting of 0.1% formic acid in Milli-Q water (both HPLC grade, Merck, Darmstadt, Germany) and solvent B being methanol (UHPLC grade ...
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bioRxiv - Microbiology 2023Quote: ... Samples were passed through a 0.2 µm nylon membrane prior to analysis with liquid chromatography as described previously 50. Separation for organic acid quantification (i.e. SCFAs and intermediate metabolites) was conducted on a LaChrom HPLC-System (Merck-Hitachi, Japan) using a SecurityGuard Cartridges Carbo-H (4 × 3.0 mm ...
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bioRxiv - Cell Biology 2024Quote: Treatment of flies with antioxidant Vitamin C was performed by feeding male flies with food containing 100mM Vitamin C (L-ascorbic acid; Merck – A4403) for the two days before dissection.
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bioRxiv - Microbiology 2024Quote: ... Samples for VFA determination were further fixed by adding 1% v/v of 4 M sulfuric acid (H2SO4, Merck Millipore, Germany). The concentrations of VFAs and sulfate were determined using a 930 Compact IC Flex 1 with a Metrosep A supp 7 −250/4.0 column coupled with an 887 Professional UV/VIS detector (Metrohm ...
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bioRxiv - Biochemistry 2024Quote: ... and E105 amino acids were mutated to Alanine using the Quick-change site-directed mutagenesis via KOD hot start DNA polymerase (Merck Millipore). For TON_0321 expression ...
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bioRxiv - Cancer Biology 2024Quote: ... cell culture media was changed to FCS-free high glucose DMEM containing 12.5 µM 13C16-labelled or unlabelled palmitate conjugated to 2% fatty acid-free bovine serum albumin (BSA, Merck Life Sciences). Palmitate was prepared in cell culture media from 100 mM ethanolic stocks of palmitic acid and conjugated to fatty acid-free BSA by incubating at 55°C in media for 2 h ...
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bioRxiv - Cell Biology 2024Quote: ... The digestion was quenched by lowering the pH with formic acid (Fisher) and the sample was desalted with a ZipTip C18 (Merck-Millipore) column ...
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bioRxiv - Plant Biology 2021Quote: ... Extracted peptides were dried in a speed-vac and resuspended in (v/v) 2% acetonitrile/0.2% trifluoroacetic acid (Merck, catalog no. 302031). A total of four biological replicates for each sample type was submitted.
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bioRxiv - Synthetic Biology 2022Quote: ... All yeast transformations were plated on synthetic complete (SC) plates containing 1.7 g/L yeast nitrogen base without amino acids and ammonium sulphate (Sigma Aldrich, Merck Life Science), 1 g/L monosodium glutamate (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Protein transfer was then visualized using 0.1 % Ponceau S solution (w/v) in 5% acetic acid (Merck Life Sciences, Cat #: P7170-1L) and imaged ...
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bioRxiv - Synthetic Biology 2023Quote: ... VpALDH and SlAlaR were combined (each at 0.05 μM) with 1 U mL−1 D-amino acid oxidase (DAAO) from porcine kidney (Merck product no. A5222) and 2 U mL−1 catalase from bovine liver (Merck-MilliPoreSigma ...
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bioRxiv - Molecular Biology 2024Quote: The frozen brain tissue samples were first thawed on ice and homogenized in 25 volumes (w/v) of ice-cold 0.1 M perchloric acid (Merck KGaA, Darmstadt, Germany) using an ultrasound homogenizer (Soniprep 150 ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were loaded onto the nanoACQUITY UPLC Trapping Column (Waters Corporation, Milford, MA, USA) using a mobile phase consisting of water containing 0.1% formic acid (Merck KGaA, Darmstadt, Germany). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... formic acid (FA, 56302), DL-dithiothreitol (DTT, D5545), iodoacetamide (IAA, I1149) and calcium chloride (CaCl2, A4689) were purchased from Merck (Darmstadt, Germany). Sequencing grade trypsin LC-MS grade was purchased from Promega (#V5111 ...
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bioRxiv - Cell Biology 2024Quote: ... resuspended in 50 µl of water and thoroughly mixed at 1:1 volumetric ratio with matrix solution (2,5-Dihydroxybenzoic acid) (Merck KGaA, Darmstadt, Germany). One µl of each sample was deposited onto the MALDI (Matrix Assisted Laser Desorption Ionization ...
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bioRxiv - Microbiology 2021Quote: ... cells and tissue were washed 3 times with phosphate buffered saline (PBS) + 0.02M glycine (Merck, K27662101) to remove fixative ...
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bioRxiv - Biophysics 2021Quote: ... Cells at 2-3 x 106 cells/mL were induced with 50 ng/mL doxycycline (Merck) and 5 mM valproic acid (Cayman Chemical) ...
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bioRxiv - Microbiology 2021Quote: ... Supernatant was transferred to Amicon Ultra-0.5 Centrifugal Filter Unit 3 kDa (Merck Millipore cat. #UFC500396) and centrifuged for 45 minutes at 4°C at 12,000g ...
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bioRxiv - Microbiology 2021Quote: ... Supernatant was transferred to Amicon Ultra-0.5 Centrifugal Filter Unit 3 KDa (Merck Millipore cat # UFC500396) and centrifuged for 45 minutes at 4°C at 12,000 g ...
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bioRxiv - Biochemistry 2022Quote: ... 50 μl of each aliquot were transferred into a 500 μl 3 kDa filtration unit (Merck) and immediately 450 μl of PBS supplemented with protease inhibitors were added to each aliquot ...
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bioRxiv - Microbiology 2020Quote: ... The released antigen was then concentrated with the Amicon 3 kDa filter (Merck KGaA, Darmstadt, Germany) before being analysed by SDS gel electrophoresis ...
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bioRxiv - Microbiology 2021Quote: ... Supernatant was transferred to Amicon Ultra-0.5 Centrifugal Filter Unit 3 KDa (Merck Millipore cat # UFC500396) and centrifuged for 45 min at 4°C at 12,000 g ...
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bioRxiv - Microbiology 2021Quote: ... Supernatant was transferred to Amicon Ultra-0.5 Centrifugal Filter Unit 3 kDa (Merck Millipore cat # UFC500396) and centrifuged for 45 minutes at 4°C at 12,000 g ...
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bioRxiv - Microbiology 2021Quote: ... 1.25 μl of a 100 mM Tris(3-hydroxypropyltriazolylmethyl)amine solution (THPTA; 95% purity, Merck KGaA) and 0.3 μl of 5 mM Azide-Fluor 488 in DMSO (≥90% purity ...
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bioRxiv - Neuroscience 2021Quote: ... This was followed by a 3 h blocking step with 5 % NGS (G9023, Merck, Darmstadt Germany) in the respective washing buffer ...
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bioRxiv - Immunology 2020Quote: ... 2-3 × 105 cells/well were plated in an ELI Spot plate (MAHAS4510, Merck Millipore, USA) and in vitro cultured for 18-24 hours in media supplemented with or without peptide at 0.5 µM (or ...
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bioRxiv - Molecular Biology 2020Quote: The capped-RNA after nsp10-nsp16 reaction was digested using 3 U of Nuclease P1 (Merck) in 50 mM ammonium acetate buffer (pH 4.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE; Corden Pharma, Plankstadt Germany) and Cholesterol (Merck Millipore, Germany) by a thin-film method modified from previously described by Wui (Wui et al. ...
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bioRxiv - Biochemistry 2020Quote: ... then supernatant concentrated to 2 mL using 3 kDa Amicon Ultra-15 Centrifugal Filter Units (Merck). Supernatant was then washed 3 times with 15 mL 10 mM ammonium acetate to remove residual erythromycin ...
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bioRxiv - Cell Biology 2021Quote: ... and were passed through a polycarbonate membrane filter with a 3-µm pore size (Merck Millipore). The supernatants (lysates ...
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bioRxiv - Cell Biology 2021Quote: ... Purified proteins were were concentrated > 60 μM using Amicon Ultra centrifugal filters (Merck; MWCO 3 kDa), rebuffered to 50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... After binding beads were washed 3 times with binding buffer (PBS with protease inhibitor cocktail (Merck) and 1 mM Na3VO4 ...