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Citations for Merck :
751 - 800 of 1360 citations for Recombinant Human CRYAB Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Resolved proteins were then electrophoretically transferred onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, USA) at 90 V for 2.5 h ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was concentrated with an Amicon Ultra-4 (Merck Millipore, MWCO 3 K) to a concentration of 726 µM ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were diluted with 90% methanol/100 mM triethylammonium bicarbonate (TEAB) (Merck Darmstadt, Germany) for efficient trapping in Micro S-Trap columns (ProtiFI ...
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bioRxiv - Physiology 2023Quote: Proteins were extracted from powder on ice using lysis buffer [150 mM NaCl (Merck), 10 mM Tris/HCl (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein was then transferred to 0.4 μm polyvinylidene fluoride (PVDF, Merck Millipore) membrane ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred onto a PVDF membrane (Immobilon-P, Merck Millipore, Massachusetts, USA) and the membranes were blocked with PBS containing 5% non-fat dry milk for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... Protein expression was performed in the presence of 3.5 mM valproic acid (Merck Millipore) and 10 μM kifunensine (Biomol) ...
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bioRxiv - Developmental Biology 2023Quote: ... NuPage 4 to 12% bis-tris protein gels (NP0323, Thermo Scientific; or MP42G15; Merck) before transferring into 0.22μm pore size polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentration was adjusted by concentrating of the samples on 100kDa filters (Amicon, Merck). Samples were loaded on 10% SDS-PAGE gel and SPATEs were visualized by Coomassie Blue staining (Instant Blue ...
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bioRxiv - Cell Biology 2023Quote: ... the protein was concentrated using a 50 kDa cut-off Amicon filter (Merck Millipore) and loaded onto a pre-equilibrated Superdex 200 Increase 10/300 GL column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: ... the protein was concentrated using a 50 kDa cut-off Amicon filter (Merck Millipore) and loaded onto a pre-equilibrated Superdex 200 Increase 10/300 GL column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: ... SDS-PAGE and protein transfer to polyvinylidene difluoride (PVDF) membrane (Immobilon-P, Merck Millipore) was performed with the NuPAGE Novex and XCell II Blot Module systems (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were concentrated by ultrafiltration on 10K Amicon® Ultra Centrifugal Filters (Merck Millipore), aliquoted ...
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bioRxiv - Plant Biology 2024Quote: ... Purified proteins were concentrated using 10 kDa ultra-centrifugal filter Amicon (Merck Millipore, Germany). The quality and quantity of proteins were analyzed by SDS-protein gel electrophoresis (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... was subjected to affinity purification using S-protein-agarose (Merck Millipore, Burlington, MA, U.S.A.) for 1– 2 h and washed 4 times ...
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bioRxiv - Biophysics 2024Quote: ... we blotted the proteins at RT on a PVDF membrane (Merck, Cat. No.: IPVH00010). We blocked the membranes for 1 h at RT with sterile filtered 5 % bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were eluted using 44 μl FLAG-peptides (200 μg/ml; Merck/Sigma Aldrich) in 1x TBS ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were concentrated using Amicon Ultra-15 Centrifugal Filter Units 30 kDa MWCO (Merck).
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bioRxiv - Neuroscience 2024Quote: ... and immunoreactive proteins were visualized using Immobilon® Forte Western HRP Substrate (Merck Millipore) at the Imager Chemi Premium (VWR International) ...
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bioRxiv - Genetics 2024Quote: ... Proteins were separated by SDS-PAGE using 4-12% Bis-Tris gels (Merck, #MP41G12) and transferred onto an activated PVDF membrane (Merck ...
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bioRxiv - Biochemistry 2020Quote: ... cells were stimulated for the indicated periods of time with antibodies to human CD3 (7.5 μg/mL, Cat. number 217570, Merck; Jurkat cells) and EGF (50 ng/mL ...
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bioRxiv - Physiology 2019Quote: ... following which they were treated with media containing 20% human serum (fed or fasted) and 1 µM puromycin (Merck Millipore Limited) for a further 4 h ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... PAb directed against phospho-STAT2 (Tyr689) and MAb directed against human IFNα/β receptor chain 2 (MAB1155) were purchased from Merck-Millipore.
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bioRxiv - Biochemistry 2021Quote: ... These Wild Type or Mutant Type let-7a-5p plasmids were co□transfected into treated human chondrocytes cells along with NC mimics or KCNQ1OT1 mimic (Sigma□Aldrich; Merck KGaA) using Lipofectamine 6000 following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and the Human Cytokine/Chemokine Magnetic Bead Panel (Cat# HCYTMAG-60K-PX30) on a Luminex MAGPIX system (Merck Millipore, Billerica, MA). Conditioned media concentrations (pg/mL ...
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bioRxiv - Microbiology 2022Quote: ... Aliquots of them were analyzed with the MILLIPLEX human cytokine/chemokine magnetic bead panel (HCYTOMAG-60K, Merck Millipore, Burlington, MA, USA) using the Luminex 200 multiplexing instruments (40-012 ...
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bioRxiv - Molecular Biology 2020Quote: ... mt1 and mt2 were generated by inserting the ORF of human TruB1 and its mutants into the pET22b vector (Merck Millipore) at the NdeI and XhoI sites ...
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bioRxiv - Neuroscience 2021Quote: ... pSIH-mCherry-KDEL construct expressing lentiviruses were added to the suspension of motoneurons before plating on polyornithine-(PORN) and human merosin-coated (CC085, Merck-Millipore) plates ...
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bioRxiv - Microbiology 2021Quote: ... Cytokine quantification was performed using a Human Cytokine/Chemokine/Growth Factor Panel A 48-Plex Premixed Magnetic Bead Multiplex Assay (Merck Millipore), using the Luminex MAGPIX System in 96-well plate format ...
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bioRxiv - Neuroscience 2022Quote: Human Neural Stem Cells (hNSCs) derived from the NIH approved H9 (WA09) human embryonic stem cell line were purchased from Merck (Germany). The hNSCs were plated on matrigel-coated 100 mm petri dishes and maintained in culture with NSCs expansion media (KO DMEM/F12 ...
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bioRxiv - Cell Biology 2022Quote: ... human very-low-density and low-density lipoproteins (catalog no. 437647-5MG and 6 mg/ml, LP2-2MG, Merck Millipore, respectively) were purchased and stored according to the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2024Quote: ... MACS-enriched from fresh PBMC were seeded at 0.5×106 cells/cm2 in RPMI plus 5% heat-inactivated human AB serum (Merck Life Sciences) and 100 U/ml penicillin-streptomycin and cultured with 50 ng/ml rhIL-4 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Primary human T-cells were isolated from peripheral blood (NHS Blood & Transplant Service, UK) using Ficoll-PaqueTM (Merck Group, Darmstadt, Germany) density gradient centrifugation followed by positive selection using antibody-coated magnetic beads directed against either human CD4 or CD8 antigen (Miltenyi Biotechnology ...
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bioRxiv - Cancer Biology 2024Quote: CM was collected from TIS MDA-MB-231 cells for chemokine and cytokine analyses using a Milliplex MAP human cytokine/chemokine magnetic bead panel (Merck Millipore) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... and PBMCs (1 × 106 cells per well) were seeded in 96-well plates in RPMI supplemented with 1% PNS and 10% AB human serum (Merck, UK) and stimulated with SARS-CoV-2 specific peptides pools ...
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bioRxiv - Developmental Biology 2023Quote: ... a micro-infusion pump (iPRECIO SMP-200, ALZET Osmotic Pumps) with 15 IU/kg human follicle-stimulating hormone (hFSH, Gonal-f; Merck Biopharma) was embedded subcutaneously under anaesthesia and injected 7 µL/h for 10 days ...
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bioRxiv - Molecular Biology 2020Quote: ... Total protein concentration in eluates was measured using Direct Detect infrared spectroscopy (Merck, Darmstadt, Germany).
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bioRxiv - Cell Biology 2019Quote: ... Proteins were concentrated with Amicon Ultra-15 Ultracel 10k centrifugal filter units (Merck Millipore, #UFC901024). Purification of cytoplasmic actin ...
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bioRxiv - Pathology 2022Quote: ... XO Activity was expressed as units/mg protein using native XO from buttermilk (Merck Millipore) as standard.
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bioRxiv - Molecular Biology 2021Quote: ... Protein-G fast flow bead resin and Immobilon-P PVDF membrane were purchased from Merck-Millipore (USA) ...
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bioRxiv - Molecular Biology 2020Quote: ... the separated proteins were transferred onto a polyvinylidene fluoride membrane (Immobilon R, Merck Millipore Ltd) for 1 hour at 20 V ...
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bioRxiv - Biophysics 2019Quote: ... the protein sample was filtered using a 30 kDa centricon filter (Merck Millipore, Darmstadt, Germany). The crude protein was then injected into a DEAE anion exchange column equilibrated with 10 mM Tris (pH 7.4 ...
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bioRxiv - Molecular Biology 2019Quote: ... Total protein of EECs was extracted with 1X RIPA buffer (20–188, Merck, Darmstadt, Germany), supplemented with Halt™ Proteinase & Phosphatase Single-Use inhibitor cocktail (78442 ...
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bioRxiv - Immunology 2020Quote: ... the Protein G chip was regenerated for 30 seconds with 100 mM Glycin-HCl (Merck) pH 1.5 at 30 µL/min and the process was repeated starting a new cycle with the capture of the potentiating antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Equal loading of protein samples on the membrane was confirmed using Ponceau S (MERCK, P3504) staining (2% Ponceau S (w/v ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were concentrated to 12 mg/mL using an Amicon Ultra Centrifugal Filter (Merck-Millipore). To purify non-degraded PrgA (without the S476A mutation) ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were transferred to a 0.45 μm pore size polyvinylidene fluoride transfer membrane (Merck Millipore), and nonspecific binding was blocked in Tris-buffered saline/0.1% Tween 20 with 5% skim milk powder ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were concentrated using Ultra-15 mL centrifugal filter devices (Amicon, Merck KGaA, Darmstadt, Germany) with a molecular weight cut-off (MWCO ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were transferred from the polyacrylamide gel to a PVDF membrane (Merck Millipore, Massachusetts, USA). Membranes were blocked with Rockland blocking buffer (Biomol GmbH ...
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bioRxiv - Cell Biology 2021Quote: ... Intracellularly applied drug: the membrane impermeable G-protein blocker GDP-β-S (2 mM, Merck) (Farkas et al. ...