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Citations for Merck :
751 - 800 of 4732 citations for Polyhomeotic Like Protein 1 PHC1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... the proteins were transferred on a nitrocellulose membrane (Amersham, Merck,) which was probed over-night at 4°C with specific antibodies diluted in 1-3% skimmed milk-PBS-0.1% Tween solution ...
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bioRxiv - Cell Biology 2022Quote: ... 30 μl Protein G Sepharose®(#GE17-0618-01, Merck) was coupled to 1 μg monoclonal mouse anti-GFP antibody (#11814460001 ...
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bioRxiv - Molecular Biology 2023Quote: ... After the transfer of proteins onto a PVDF membrane (Merck), blocking was carried out (5% skimmed milk in PBS) ...
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Docking Domain Engineering in a Modular Polyketide Synthase and its Impact on Structure and FunctionbioRxiv - Biochemistry 2023Quote: ... Proteins were concentrated with Amicon Ultra centrifugal filters from Merck Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluted proteins were concentrated using Amicon spin concentrators (Merck Millipore) and subjected to gel filtration (Superdex 75 and 200 16/60 pg ...
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bioRxiv - Immunology 2023Quote: ... Separated proteins were transferred to PVDF membranes (0.45 mm, Merck) by semi-dry transfer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and was established using the following standard proteins (Merck MWGF1000): carbonic anhydrase (CAN ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to PVDF Immobilon-FL membranes (Merck Millipore) using the Power Blotter Semi-dry Transfer System (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... 5 ml of BugBuster Protein Extraction Master Mix (Merck KGaA) was added ...
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bioRxiv - Neuroscience 2024Quote: ... samples were incubated with protein G-agarose beads (11719416001, Merck) in IP buffer overnight at 4 °C with rotation ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were electroblotted onto Immobilon-P transfer membranes (Merck). Thereafter ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were blotted onto a PVDF membrane (Merck Millipore, IPVH00010) or nitrocellulose membrane (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... proteins were transferred onto a 0.45µm PVDF membrane (Immobilon, Merck). The membranes were stained with a 1:1 mix of glacial acetic acid and Ponceau Red (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were determined using the BCA assay (Merck-Millipore) and the proteins were digested overnight at 37°C using 100:1 protein:trypsin ratio (Promega) ...
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bioRxiv - Biochemistry 2024Quote: ... the protein was concentrated using an Amicon Stirred Cell (Merck) equipped with a 100 kDa molecule weight cut off (MWCO ...
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bioRxiv - Evolutionary Biology 2024Quote: ... proteins were concentrated using 3,000 MWCO centrifugal filters (Merck, UFC500396) and protein concentrations were quantified by Bradford Assay (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... GFP (1:2000) (Faix et al., 2001) or mouse monoclonal antibody against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:1000; #CB1001-500UG, Merck (Darmstadt, Germany)) and anti-fascin antibody 5E2 (undiluted hybridoma supernatant) ...
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bioRxiv - Neuroscience 2021Quote: ... the slices were processed with 1% H2O2 in phosphate buffer to deactivate the intrinsic peroxidase and then incubated with an anti-c-Fos antibody (1:10,000, rabbit; Merck KGaA, Darmstadt, Germany) in 10% normal goat serum in phosphate-buffered saline with 0.3% Triton X-100 (PBS-X ...
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bioRxiv - Cell Biology 2021Quote: ... Blots were then reprobed with monoclonal anti-β-actin-peroxidase clone AC-15 antibody (a3854, Merck, Saint Louis, MO, dilution 1:5000) in TBS/T for 90 minutes ...
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bioRxiv - Microbiology 2020Quote: ... and probed with 1:1000 diluted primary anti-Ago2 rat monoclonal antibody clone 11A9 (Millipore Sigma/ Merck KGaA MABE 253, Darmstadt, Germany), 1:25000 diluted primary mouse monoclonal anti-beta actin antibody (AC-15 ...
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bioRxiv - Neuroscience 2022Quote: ... for 5 min each at RT and incubated with secondary antibodies in TPBS-TS-1% for 4h at 4°C (Alexa Fluor 546 anti-Mouse (Merck, A-11018), Alexa Fluor 488 anti-goat (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were washed and probed with secondary antibody for one hour at RT (anti-mouse IgG, horseradish peroxidase linked; 1:5,000; Merck, Burlington, MA, USA). Bands were visualized using high sensitivity Pierce ECL Plus (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... 0.1% (20mM Tris and 500mM NaCl pH 7.5) and incubated overnight at 4°C with the primary antibody: anti-GluN1 (1:1000; Merck KGaA, Darmstadt, Germany) and anti-GluN2A monoclonal antibody (1:1000 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The membranes were probed with anti-GFP at a dilution of 1:3000 of the antibody conjugated to a peroxidase (SAB2702198, Merck KGaA, Germany) in the blocking buffer overnight at 4 °C on a rocker ...
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bioRxiv - Neuroscience 2020Quote: ... 0.2% Tween-20 in PBS) for 1 h and then incubated with a rabbit antibody against the extracellular domain of CSF1R (Merck, 06-174,) or a mouse anti-HA tag antibody (BioLegend ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted 1:2000 and incubated overnight at 4°C followed by an secondary anti-rabbit HRP conjugated antibody (12-348, Merck Millipore, Germany) diluted 1:5000 in PBST and incubated for 1 h at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... The detection was performed using a specific secondary antibody coupled to the horseradish peroxidase (HRP): anti-mouse-HRP (1:4000, A4416 Merck, Sigma Aldrich), anti-rabbit-HRP (1:10000 ...
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bioRxiv - Bioengineering 2024Quote: ... The scaffold was then rinsed three times with PBS and incubated with Alexa Fluor™ 555 conjugated secondary goat anti-mouse IgG antibody (1:100; Merck, Germany) and CF™ 633 conjugated secondary donkey anti-rat IgG antibody (1:100 ...
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bioRxiv - Microbiology 2024Quote: ... Infection foci were visualised using the appropriate secondary antibody coupled with horseradish peroxidase (HRP; 1:5000, Sheep Anti-Mouse IgG A5906, Sigma-Aldrich, Merck, Germany) and TrueBlueTM chemical (SeraCare ...
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bioRxiv - Microbiology 2024Quote: ... Actin was detected with monoclonal mouse antibody coupled with horseradish peroxidase (HRP; 1:75,000; A3854, Sigma-Aldrich, Merck, Saint-Quentin Fallavier, France). Membranes were exposed to the appropriate peroxidase-conjugated secondary antibodies (1:5000 goat anti-guineapig IgG A108P or 1:10000 sheep anti-mouse IgG A5906 ...
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bioRxiv - Molecular Biology 2022Quote: ... Glucocorticoid Receptor antibody (#3660, Cell Signalling Technology) or control IgG antibody (#03-198, Merck Millipore) and incubated for 1 h at room temperature whilst rotating ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies used in immunoblotting were as follows: rabbit polyclonal antibody anti-GAPDH (Merck Millipore #ABS16), mouse monoclonal antibody anti-PRKAR2B (#610625 ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 900 µl supernatant was transferred to a new tube and incubated with 25 µl (1/2 * OD in µl) of mouse-anti-FLAG antibody (clone M2, Merck/Sigma-Aldrich #F1804) at 4 °C for 45 min (rocking) ...
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bioRxiv - Cell Biology 2021Quote: ... Free floating brain sections and fixed astrocyte cultures were immunostained with the following antibodies (Table S2): Mouse anti-NeuN (1:100, Merck Millipore, Darmstadt, Germany), Rabbit anti-GFAP (1:200 ...
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C followed by incubation with primary antibody: anti-tyrosine hydroxylase (TH, 1:1000, Merck Millipore, Darmstadt, Germany, AB152). After washing with TBST ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked using 2% normal goat serum and stained using the following primary antibodies in blocking solution overnight: anti-Ng2 (1:100, rabbit pAb, AB5320 Merck Millipore, Burlington, MA), anti-beta III tubulin (1:100 ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue sections from the in vivo experiment were incubated with a rabbit primary antibody for GluR2/3 (Merck Millipore; AB1506; 1:200) overnight and then with a secondary goat anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked in 5% BSA TBS-T or 5% skim milk TBS-T and incubated overnight at 4°C with the following primary antibodies diluted in 5 % BSA or 5% skim milk dissolved in TBS-T: pSer293-PDH (1:1,000) (Merck Millipore, Cat. no. ABS204), PDH (1:1,000 ...
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bioRxiv - Immunology 2023Quote: ... The embryos were subsequently incubated overnight at 4 °C in solution containing Cy3-conjugated α-smooth muscle actin monoclonal antibody (1:100, C6198; Merck, Darmstadt, Germany), Alexa647-Phalloidin (1:200 ...
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bioRxiv - Genomics 2024Quote: ... with the following primary antibodies: goat anti-IL1RN (RD Systems Cat # AF280NA) in 1:1000 in blocking solution and mouse anti-β actin (MERCK Cat # A1978) and rabbit anti-vinculin (Cell Signaling Cat #18799 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were blocked 1h in 10% donkey serum in PBST and incubated overnight with primary antibodies for anti-GFAP (1:250, Merck Life Science, Hpa056030) and anti-MAP2 (1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were placed in a humid chamber and cells were incubated overnight at 4°C with the following primary antibodies diluted in blocking solution: mouse monoclonal anti-FLAG clone M2 (Merck F1804; 1:1000), rabbit monoclonal anti-KTN1 clone D5F7J (Cell Signaling Technology #13243 ...
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bioRxiv - Biophysics 2021Quote: ... Pooled protein fractions were concentrated with Amicon-Ultra-15 (Merck KGaA) to 11.2 mg ml−1 as measured by the absorbance at 280 nm ...
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bioRxiv - Cell Biology 2020Quote: ... Separated proteins were transferred to PVDF-FL transfer membrane (Merck Millipore) at 40V for 90 min at 4 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined by Direct Detect® Infrared Spectrometer (Merck).
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bioRxiv - Cell Biology 2022Quote: ... was used to transfer proteins to PVDF Immobilon membranes (Merck Millipore). Blocking and antibody incubation steps were performed in EveryBlot Blocking Buffer (BioRad) ...
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bioRxiv - Neuroscience 2020Quote: ... the protein solution was filtered through 100 kDa filter (MERCK, MRCFOR100). The filtrate was transferred to a low-protein binding tube and 30 equivalents of HNE (Cayman Chem ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was concentrated in Amicon centrifugal filters (10 KDa, Merck) in presence of 1:10 GCDCA ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoreactive proteins were visualized by Immobilion western chemiluminescence substrate (Merck-Millipore). Densitometry was carried out by FIJI.
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bioRxiv - Cell Biology 2021Quote: ... The proteins were transferred to an Immobilon-P Transfer Membrane (Merck), blocked with 10% Blocking One (nacalai tesque) ...