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Citations for Merck :
701 - 750 of 1330 citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... The MNase reaction is stopped by adding 2 mM EGTA (Sigma-Aldrich/Merck). Afterwards ...
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bioRxiv - Biochemistry 2022Quote: ... transferred and tris-(2-carboxyethyl)-phosphin (TCEP, Merck, Sigma-Aldrich, 646547-10×1ML) added up to 1 mM and cysteine reduction performed for 30 min at 60°C ...
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bioRxiv - Plant Biology 2022Quote: ... coli strains derived from BL21(DE3) and Rosetta 2 (Merck Millipore, Burlington, USA), BL21(DE3)-R3-pRARE2 and BL21(DE3)-R3-lambda-PPase ...
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bioRxiv - Microbiology 2022Quote: ... the samples were reduced by 1 mM tris(2-carboxyethyl)phosphine (TCEP, Merck) and free sulfhydryl groups carbamidomethylated using 5.5 mM chloroacetamide (Sigma-Aldrich) ...
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Caloric restriction prevents inflammation and insulin dysfunction in middle-aged ovariectomized micebioRxiv - Physiology 2023Quote: ... 400 µL of 1% 2-thiobarbituric acid (TBA; Merck, St. Charles, MO, USA), and 200 µL of 20% phosphoric acid and heated at 100 °C for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... A 2 % chitosan solution was obtained by dissolving chitosan (high purity, 740063 Merck) in milliQ water with 0.13 % (vol/vol ...
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bioRxiv - Cancer Biology 2024Quote: ... H & L Chain Specific Peroxidase Conjugate (Merck, Cat#: 401315-2 ML, 1:5000). Proteins were detected by chemiluminscent detection system (Tanon ...
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bioRxiv - Cancer Biology 2024Quote: ... H&L Chain Specific Peroxidase Conjugate (Merck, Cat#: 401215-2 ML, 1:5000), or Goat Anti-Rabbit IgG ...
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bioRxiv - Microbiology 2024Quote: ... was obtained by using 4’,6-di-amidino-2-phenyl-indole (DAPI; Merck KGaA - Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... 5 and 2 minutes respectively (Elastin van Gieson staining kit, Merck, Melbourne, Australia). Antigen retrieval methods have been previously described 128 ...
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bioRxiv - Neuroscience 2024Quote: ... 2 was determined by staining with the DNA dye Hoechst 33258 (861405, Merck) as described previously (Saga et al. ...
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bioRxiv - Immunology 2024Quote: ... Dimethyl 2-oxoglutarate (DM-OG) was purchased from Merck (cat no: 349631-5G) and added to the culture medium at 2mM and 4mM final concentrations.
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bioRxiv - Synthetic Biology 2024Quote: ... followed by day 2 inoculation into 1 mL overnight autoinduction media (Merck, USA) with 50 µg/mL kanamycin ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 x 5 mins in PBS with 0.2% Triton X-100 (Merck, T8787) and 0.2% BSA (PBT ...
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bioRxiv - Neuroscience 2020Quote: Recombinant GST-hSLP4A was expressed in and purified from BL21 Rosetta DE3 E.coli (Merck, Ca. No. 70954). 5ml of saturated pDEST15 hSlp4-a-transformed BL21 Rosetta DE3 E.coli culture were diluted in 500 ml 2x YT (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: Full-length CA sequences derived from pNL4-3 was introduced to pET30a vectors (Novagen-Merck KGaA, Germany), producing a pET30a CA vector ...
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bioRxiv - Microbiology 2024Quote: Parasites were treated with 50 μM of RNA Pol III inhibitor CAS 577784-91-9 (Calbiochem, Merck) after sorbitol treatment at 3 ±3hpi and RNA was harvested at 24hpi in parallel with untreated and control samples ...
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bioRxiv - Microbiology 2022Quote: ... 5 mg/ml Hemin (Mercury Cat#3741) and 5ul/ml vitamin K1 (Merck Cas#84-80-0)) under anaerobic conditions.
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bioRxiv - Cell Biology 2020Quote: ... pDEST15 plasmids were transformed in Rosetta™ 2(DE3)pLysS Competent bacteria (Merck Millipore). Bacteria cultures were grown with the proper antibiotic at 37°C until an OD of 0.6 was obtained ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies used were CYFIP1/2 (Sra-1/PIR121 [14], Rac1/3 (23A8, Merck), Nap1 [14] ...
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bioRxiv - Microbiology 2021Quote: ... Freeze substitution was done in dry acetone containing 2% osmium tetroxide (Merck, Darmstadt, Germany) and 2% water with an AFS (Leica microsystems ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on a 2 ml HIS-SelectTM Nickel Affinity Gel (Merck) and washed with wash buffer ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on a 2 ml HIS-SelectTM Nickel Affinity Gel (Merck) and washed with wash buffer ...
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bioRxiv - Neuroscience 2021Quote: ... concentrated using 100 kDa-MWCO Centricon plus-20 and Centricon plus-2 (Merck-Millipore), aliquoted and stored at -80 °C ...
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bioRxiv - Neuroscience 2021Quote: ... 2 mM EDTA and 200 μg/ml PMSF) supplemented with protease inhibitors (Merck Chemicals) and centrifuged at 800x g for 10 min at 4°C to result in P1 and supernatant S1 ...
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bioRxiv - Neuroscience 2021Quote: ... Anti-human Aβ mouse monoclonal antibody (clone W0-2; Merck Chemicals GmbH, Darmstadt, Germany) and rabbit anti-β-actin monoclonal antibody (clone 13E5 ...
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bioRxiv - Neuroscience 2021Quote: ... and lysed directly in 15 µl 2x Laemmli buffer containing 10% 2-mercaptoethanol (Merck). Samples were loaded on a 4-to-20% SDS polyacrylamide gel (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (#D9542, Merck. Darmstadt. Germany).
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bioRxiv - Cell Biology 2021Quote: ... Differentiation of 4D7 and 2M12 cells was induced by 2% dimethyl sulfoxide (DMSO; Merck) for 48 hr ...
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bioRxiv - Cell Biology 2021Quote: ... and after 24 h the transformants were selected with 2 μg/mL puromycin (Merck) for 24–48h.
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bioRxiv - Microbiology 2021Quote: ... 2 mM β-mercaptoethanol) supplemented with a protease-inhibitor cocktail (cOmplete, Sigma-Aldrich/Merck) and stored at 193 K ...
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bioRxiv - Developmental Biology 2022Quote: ... (2) we added 50 µg/mL (0.28 collagenase Wünsch units) of Liberase TM (Merck) in the digestion solution ...
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bioRxiv - Physiology 2022Quote: ... cultures were fixed with 4% PFA and stained with 2% Alizarin red S (Merck). For the quantification ...
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bioRxiv - Developmental Biology 2020Quote: ... mice were injected intraperitoneally with a filter sterilized solution of 2 mg doxycycline (Merck), dissolved in PBS (100 μl or 200 μl of a filter sterilized ...
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bioRxiv - Microbiology 2021Quote: ... in 2 ml tubes containing 0.3 mm diameter glass beads (Merck KGaA, Darmstadt, Germany). The mixture was vortexed ...
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bioRxiv - Biochemistry 2021Quote: Transformation of Escherichia coli strain BL21(DE3) Rosetta-2 pLysS (Novagen Merck, Darmstadt Germany) were made with a pET3d-His6-CrFBA3 plasmid ...
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bioRxiv - Physiology 2021Quote: ... a grid was briefly placed on 10 μL 2% uranyl acetate (w/v, Merck). Images were acquired under a JEM-1010 electron microscope (JEOL ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplification was done using 2 ng of DNA and KOD hot star (MercK). PCR fragments were sequenced by EurofinsGenomics ...
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bioRxiv - Biochemistry 2023Quote: ... 100mM NaCl using 3k cut-off Amicon® Ultra 2 mL Centrifugal Filters (Merck). Equal amounts of protein from both dialyzed and undialyzed samples were treated with caspase-3 as described above ...
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bioRxiv - Plant Biology 2023Quote: ... then 2% w/v Aspegillus niger pectinase (Merck Life Science UK Ltd., Gillingham, UK) 45°C for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay was used to analyze the cell viability of SH-SY5Y cells ...
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bioRxiv - Biophysics 2023Quote: Snap-Streptavidin-WA-His (pETplasmid) was expressed in Rosetta 2 (DE3) pLysS (Merck, 71403). Culture was grown in TB medium supplemented with 30 µg/ml kanamycin and 34 µg/ml chloramphenicol ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted samples were concentrated using an Amicon Ultra-2 centrifuge filter unit (Merck) and stored on ice.
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bioRxiv - Molecular Biology 2023Quote: ... CaCo-2 cell lines were cultivated in Minimum Essential Medium Eagle (MEM) (Merck, M4655) complemented with 20% FBS Tetracycline free ...
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bioRxiv - Developmental Biology 2023Quote: ... except for a different set of SYBR green primers (Sigma Merck, Supplementary Table 2). The obtained Ct values were analyzed using the ddCt method for relative quantification of mRNA expression derived from three independent experiments ...
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bioRxiv - Neuroscience 2023Quote: ... concentrated using 100 kDa-MWCO Centricon plus-20 and Centricon plus-2 (Merck-Millipore), aliquoted and stored at -80°C ...
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bioRxiv - Neuroscience 2022Quote: ... 1% streptomycin and 2 mM of Glutamax and 0.1 μg·ml−1 GDNF (#SRP3200, Merck) (Vyas et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by 2 layers of SCX strong cation exchange Empore™ SPE Disks (Merck). Samples were then lyophilized.
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bioRxiv - Neuroscience 2023Quote: ... The SDC was precipitated from the combined samples using 2% formic acid (FA, Merck).
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bioRxiv - Developmental Biology 2022Quote: ... embryos were initially incubated with 100 µM EdU (5-ethynyl-2’-deoxyuridine) (Merck, T511285) for 1 h before washing and fixation ...