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Citations for Merck :
701 - 750 of 1415 citations for Recombinant Human RELT Protein hIgG His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Proteins were concentrated using a centrifugal filter unit (3 kDa MWCO, Merck Millipore) at 3500 rpm and 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... The concentrated protein was filtered through a 0.22 μm centrifugal filter (Merck Millipore) prior to loading onto the gel filtration column ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... proteins were transferred to a 0.2 μm PVDF membrane (#ISEQ85R; Sigma-Aldrich/Merck) by electroblotting the separated proteins for 1 h at 100 V ...
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bioRxiv - Plant Biology 2022Quote: ... proteins were transferred to an Immobilon®-P PVDF membrane (Merck Millipore Ltd.) in a Biometra Fastblot B34 chamber at 1 mA/cm2 membrane for 1.5 h in transfer buffer (20 mM CAPSO pH 11.2 ...
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bioRxiv - Microbiology 2022Quote: Proteins were precipitated from the eluates by adding 1.8 mL LiChrosolv ethanol (Merck) and L 1LµL GlycoBlue (Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... The protein-containing fractions were pooled and concentrated in Amicon Ultra units (Merck). Protein yields were about 20 mg L-1.
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bioRxiv - Microbiology 2022Quote: ... Pure Cas protein was finally concentrated in Amicon Ultra centrifugal filter (Merck-Millipore).
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bioRxiv - Molecular Biology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany). Unspecific binding on the PVDF membrane was blocked by incubation with the blocking buffer (5% non-fat milk ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... Proteins were analyzed by SDS-PAGE and transferred onto PVDF membrane (Merck Millipore). The membranes were washed with PBS 1x containing 0.1% Tween-20 (PBS-T ...
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bioRxiv - Molecular Biology 2022Quote: ... The purified protein was concentrated using an Amicon Ultra 100 K filter (Merck Millipore ...
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bioRxiv - Plant Biology 2024Quote: ... Protein pellets were resuspended in 50 μl of 1.5% sodium deoxycholate (SDC; Merck) in 0.2 M EPPS-buffer (Merck) ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were concentrated using a 10K Amicon tube (Merck-Millipore, Darmstadt, Germany) and the buffer was changed to PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were concentrated using a 10K Amicon tube (Merck-Millipore, Darmstadt, Germany) and the buffer was changed to PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... protein pellets were resuspended in 50 µl of 1.5% sodium deoxycholate (SDC; Merck) in 0.2 M EPPS-buffer (Merck) ...
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bioRxiv - Microbiology 2024Quote: ... The pellet was resuspended in protein resuspension buffer (150 mM NaCl (Merck, USA), 5 mM EDTA (Merck ...
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bioRxiv - Biochemistry 2024Quote: ... Immunoreactive proteins were visualized using Immobilon® Forte Western HRP Substrate (Merck Millipore) at the Imager Fluor-Chem M (Proteinsimple) ...
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bioRxiv - Developmental Biology 2023Quote: ... and separated proteins were then transferred to PVDF membranes (polyvinylidene difluoride, Merck Millipore). After treatments with a blocking reagent (Blocking One solution ...
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bioRxiv - Molecular Biology 2023Quote: Proteins separated by SDS-PAGE were transferred to Immobilon-P membranes (Merck Millipore) utilizing a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins separated by SDS-PAGE were transferred to Immobilon-P membranes (Merck Millipore). Three major bands on the membrane were cut and subjected to N-terminal Edman sequencing by Hokkaido System Science Co. ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved by SDS–PAGE and transferred to nitrocellulose membranes (Amersham, MERCK). Membranes were blocked with Odyssey Blocking Buffer (LI-COR ...
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bioRxiv - Cancer Biology 2023Quote: ... protein lysates were prepared using 1X RIPA buffer containing protease (#539131, Merck Millipore) and phosphatase (#524629 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein pellets were resuspended in 50 µl of 2.5% sodium deoxycholate (SDC; Merck) in 0.2 M EPPS-buffer (Merck) ...
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bioRxiv - Biochemistry 2023Quote: ... Then the protein was concentrated with centrifugal concentrators (UFC901096, Merck Millipore, Cork, Ireland), and loaded onto a SuperoseTM 6 Increase 10/300 GL gel-filtration column (29- 0915-96 ...
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bioRxiv - Cell Biology 2023Quote: Saporin was labelled with an ATTO 550 protein labelling kit (Merck, 51146-1KT).
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bioRxiv - Cell Biology 2023Quote: Wet blot transfer was used to transfer proteins to a PVDF membrane (MERCK). Intercept blocking buffer (927-60001 ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated with first and secondary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were resolved in SDS-PAGE gels and electrotransferred onto nitrocellulose membranes (Merck). After incubation with the appropriated primary antibody ...
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bioRxiv - Microbiology 2023Quote: ... Then the protein sample was concentrated using an Amicon Ultra centrifugal tube (Merck) with a buffer change to phosphorylation buffer 2X (100 mM Tris pH 8 ...
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bioRxiv - Plant Biology 2023Quote: ... after concentrating the protein (Amicon® Ultra MWCO 30 kDa, Merck, Darmstadt, Germany), a second GST-tag purification was performed and the peak fractions were pooled and concentrated (Amicon® Ultra MWCO 10 kDa ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were blotted on the Immobilon-P PVDF 0.45 µm (MERCK, Darmstadt, Germany). PVDF membranes were blocked using the PVDF Blocking Reagent for Can Get Signal (TOYOBO) ...
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bioRxiv - Microbiology 2023Quote: ... and the protein concentration was determined by bicinchoninic acid (BCA) assay (Merck Millipore) and by UV-visible spectroscopy using an ε of 24 mM⁻¹·cm⁻¹ at 280 nm ...
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bioRxiv - Physiology 2023Quote: ... and protein concentration in the supernatant was determined by BCA assay (Merck B9643). Finally ...
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bioRxiv - Biochemistry 2023Quote: ... The 139H2 was purified with Protein G Sepharose 4 Fast Flow beads (Merck), washed with PBS ...
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bioRxiv - Physiology 2023Quote: ... a specific inhibitor targeting the alpha subunit of the Gi/o protein (Merck); U73122 ...
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bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were concentrated by centrifugal filters (Amicon Ultra 10K, Merck Millipore Ltd.). Protein concentrations were determined by a Pierce™ BCA Protein Assay Kit (Thermo Fischer Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were filtered using an Ultrafree 0.22 μm centrifugal filter (Merck Millipore) to remove any aggregation ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50 µM Rho-associated protein kinase (ROCK) inhibitor (Merck Millipore, SCM-075). On day 3 ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were transferred to 0.45 µm PVDF membranes (Merck Millipore, cat.no. IPVH00010). After blocking unspecific binding sites of the membrane at RT for 1 h with 5 % skim milk powder in TBS-T ...
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bioRxiv - Cell Biology 2023Quote: Caco-2 cells were co-transfected with eSpCas9-GFP protein (Merck, cat #ECAS9GFPPR) and sygRNA (Merck ...
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bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... Proteins were transferred onto a 0.45 µm PVDF Immobilon-P membrane (Millipore, Merck) using the Pierce Power Blotter (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... PBMCs (2 × 106 cells) were plated onto 48-well plates (NalgeNunc) in RPMI-1640 with 5% inactivated male human AB serum (Merck) for 3 hours ...
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bioRxiv - Immunology 2021Quote: ... that serves as support for the culture system was prepared seeding human dermal fibroblasts (200,000) re-suspended in a fibrin gel made of fibrinogen (Merck-Millipore), thrombin (Merck-Millipore ...
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CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: Wild-type Xenopus tropicalis females and males were primed with 20U and 10U PREGNYL© human chorionic gonadotropine (hCG) (Merck), respectively ...
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bioRxiv - Developmental Biology 2022Quote: Ovulation was induced by injecting adult female Xenopus laevis with 600 units of human chorionic gonadotropin (HCG, MERCK Animal Health) and animals were kept at 16 dc overnight ...
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bioRxiv - Bioengineering 2021Quote: Isolation of fresh human PBMCs was initiated within 1 h after blood collection using Histopaque® 1077 (10771; Merck KGaA) and standard density centrifugation (800 rcf ...
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bioRxiv - Bioengineering 2021Quote: The rat INS-1 832/3 cell line (insulinoma cell line stably transfected with human insulin; hereinafter INS-1) was obtained from Merck. A HUVEC (human umbilical vein endothelial cell ...
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bioRxiv - Cancer Biology 2021Quote: ... and SPPL2A and control siRNA (IDT) were transfected into two human cell lines (HMV-2/CVCL_1282 -Merck-, WM3211/CVCL_6797 -Rockland-) using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... At day 10 cells were passaged at a 2:3 ratio into 12 well cell culture plates coated with 15 µg/ml human plasma fibronectin (Merck) in Dulbecco’s phosphate-buffered saline (DPBS ...