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Citations for Merck :
701 - 750 of 1577 citations for Benzyl carbamic acid prop 2 ynyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... that was recently established by a disruption of both AAH1 and APT1.18 Yeast strains were grown overnight at 30 °C in a synthetic defined SD medium (6.7g/L Yeast Nitrogen Base without Amino Acids (Difco) and 20g/L glucose (Merck)) containing a specific mixture of amino acids and nucleobases ...
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bioRxiv - Bioengineering 2021Quote: ... The chemical reagents of L-cysteine (analytical reagent) and lactic acid (analytical reagent) were purchased from Merck (Darmstadt, Germany) and Fischer scientific (Merelbeke ...
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bioRxiv - Biochemistry 2020Quote: ... ammonia (25% in H2O), acetic acid (≥ 98.5%), ethanol (≥ 99.5%), and sodium hydrogencarbonate (NaHCO3, ≥ 99.5%) were purchased from Merck (Darmstadt, Germany). Tris(hydroxymethyl)amino-methane (TRIS ...
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bioRxiv - Microbiology 2020Quote: ... The LC flow rate was 300 nL/min and the 100-min linear gradient ranged from 95% solvent A (H2O with 0.1% formic acid (Merck)) to 40% solvent B (100% acetonitrile with 0.1% formic acid) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... for 10 minutes at room temperature, Ponceau (C.I.14700, FUJIFILM-Wako Pure Chemical Corp., Osaka, Japan) acid fuchsin (C.I.42685, Merck-Millipore) azofloxine (C.I.18050 ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 8.0) and then transferred to 1.5 mL tubes prewarmed at 65°C acid-washed glass beads (G8772-100 Merck) suspended in 25 µL of 10% filtered SDS and 300 µL of acid phenol chloroform pH 4.5 ...
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bioRxiv - Developmental Biology 2022Quote: ... Total protein concentration of lysates was determined using a bicinchoninic acid kit (Merck Life Science UK Ltd, Gillingham, UK) and samples diluted in Laemmli buffer ...
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bioRxiv - Neuroscience 2024Quote: ... the brain sections were thawed in a desiccator and spray-coated with 80 µl of a freshly prepared solution of 2,5-dihydroxybenzoic acid (DHB, Merck) using a SMALDIPrep ultrafine pneumatic spraying system (TransMIT GmbH) ...
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bioRxiv - Biochemistry 2023Quote: ... was incubated in 5 mM Tris-HCl buffer (pH 7.5) containing each substrate (1% glucomannan, Neogen, MI, USA; 1% polygalacturonic acid, Neogen; 1% carboxymethyl cellulose, Merck; 1% soluble starch ...
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bioRxiv - Cancer Biology 2022Quote: ... 400μL were mixed with 200μL of a 3 mg/mL collagen in 0.1% acetic acid solution (rat tail type I; Merck Millipore), followed by the addition of 10μL of 1 M NaOH ...
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bioRxiv - Neuroscience 2024Quote: ... DMSO (0.1%; D4540), L-glutamic acid (L-glutamate, 1 mM; G1251) and Sodium Propionate (5mM; P1880) were obtained from Merck Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were resuspended in 10 μL of 0.1% (v/v) formic acid and cleaned up using a zip-tip (Merck).
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bioRxiv - Molecular Biology 2024Quote: ... digested peptides were acidified by the addition of 5% (v/v) LC-MS grade Formid Acid (FA) (Merck, 5.33002.0050) and purified on C18 columns (Stagetips ...
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bioRxiv - Microbiology 2024Quote: ... Micrococcin P1 was produced as described previously and solubilized to a concentration of 10 mg/ml in dimethyl sulfoxide (Sigma-Aldrich).30 Nisin A was obtained as part of a commercial fermentate which was solubilized to a concentration of 1 mg/ml in a 0.05% acetic acid solution (Merck). AuresinePlus was solubilized to a stock concentration 1 mg/ml in a buffer containing 20 mM Tris-HCl pH 7.0 ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant aliquots were kept at -18°C before being diluted with 2.5 mM sulfuric acid (H2SO4, Merck, Darmstadt, Germany) at 1:6 ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmax supplemented with 1% v/v of the 10% fatty acid-free BSA solution in PBS (Merck cat# A1595) supplemented with 10% Ethanol (Fisher Scientific cat# 10680993 ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.5 ml nuclei wash, and suspension buffer (NWS-Buffer: 2% BSA, 2 mM MgCl2, 0.5% Protector RNAse Inhibitor (SKU3335399001, Merck, Soeborg, Denmark)) was carefully added ...
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bioRxiv - Biophysics 2022Quote: ... 2 mL of EV-containing eluate was collected and concentrated to 100 μL using Amicon Ultra-2 10K filters (Merck Millipore).
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bioRxiv - Neuroscience 2023Quote: Place 100 μl trypsin and 100 μl collagenase (per 2 animals) in a 35 mm dish: 2 mg ml-1 Trypsin (Merck - 85450C) and 682 U ml−1 Collagenase in Ca2+ and Mg2+ free medium
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bioRxiv - Cell Biology 2022Quote: ... The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck, Poznan, Polska). The samples containing 20 µg of protein were mixed with 4× Laemmli loading buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated in low serum medium (0.5% FBS) containing 20 µM retinoic acid (RA) (Sigma-Aldrich; Merck KGaA) for 5 days ...
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bioRxiv - Immunology 2021Quote: ... The digested products were then diluted with formic acid to a final concentration of 0.1% and cleaned up using ZipTip C18 (Merck Millipore). Data was obtained on an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... HPLC-grade ammonium formate (>98%) and formic acid (>98%) used in the LC mobile phase were purchased from Merck (Germany) and Sigma-Aldrich (USA) ...
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bioRxiv - Systems Biology 2020Quote: ... The resultant pellets were resuspended in 3% acetonitrile + 0.1% trifluoroacetic acid and peptide quantification performed using the Direct Detect system (Merck Millipore). Protein samples were normalized then vacuum concentrated in preparation for mass spectrometry.
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bioRxiv - Microbiology 2020Quote: ... the supernatant was then filtered through an acid-washed 0.22 μm polycarbonate filter (GSV, Life Science) using a Sterifil 47 mm filter holder (Merck Millipore). The flow-through liquid was transferred into an acid-washed 10 L polycarbonate (PC ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell pellets were digested with a standard volume of suprapur nitric acid and hydrogen peroxide (Merck Millipore, MA, USA) (2:1 v/v ...
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bioRxiv - Microbiology 2020Quote: ... Samples were stored at 4°C overnight before desilicification with 4% suprapure hydrofluoric acid (Merck; incubation of approximately 5 hours). Afterwards ...
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bioRxiv - Biochemistry 2021Quote: ... Reactions were stopped at each time point using 4 N formic acid and analyzed by PEI-Cellulose-F TLC (Merck) developed in 0.4 M potassium phosphate buffer (pH 3.4) ...
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bioRxiv - Microbiology 2020Quote: ... A was 1 mM ammonium formate pH 9 (for lincomycin detection, prepared by titration of formic acid 98–100%, Merck, Germany with ammonium hydroxide 28–30% ...
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bioRxiv - Molecular Biology 2023Quote: ... To deplete Xrn1 and Dcp2 with the AID tags (Nishimura et al., 2009; Morawska and Ulrich, 2013) 1-Naphthaleneacetic acid (1-NAA; N0640-25G, Merck) was added to a final concentration of 1 mM ...
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bioRxiv - Microbiology 2023Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 μg/mL 2,6-Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
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bioRxiv - Neuroscience 2023Quote: ... before transcardially perfusing with 21-28 ml of ice-cold PBS and 4%PFA-0,12% picric acid (Merck, Søborg, Denmark). Spleens were excised and weighed ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with 1X PBS and fixed with ice-cold 10% trichloroacetic acid (Sigma Aldrich; Merck cat. no: 27242) at dark ...
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Insight into the regulatory mechanism of the MFS transporter, SCO4121 by the MarR regulator, SCO4122bioRxiv - Molecular Biology 2024Quote: ... the MSA agar plate was flooded and 500 µg/ml of nalidixic acid (Himedia) and 1 mg/ml of apramycin (Merck). After incubation ...
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bioRxiv - Microbiology 2024Quote: ... Samples were separated on a 1.2 % agarose gel stained with 0.01 % (v/v) GelGreen Nucleid Acid Stain (Merck, Darmstadt, Germany) in TBE buffer for 2 h at 120 V ...
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bioRxiv - Immunology 2024Quote: ... The ethanol and unloaded nucleic acids in the mixtures were removed using Amicon Ultra centrifugal filters (10- or 30-kD MWCO, Merck) for two rounds with 5- and 15-times volumes of 1× PBS ...
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bioRxiv - Biophysics 2024Quote: Glass coverslips (Paul Marienfeld GmbH, 24 x 50 mm, 170 ± 5 μm) were overnight incubated in 100 mM Sulphuric acid (Merck). Afterwards the coverslips were rinsed consecutively with Milli-Q water ...
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bioRxiv - Systems Biology 2023Quote: ... UK), eosin Y 1% alcoholic and xylene (CellPath, UK), periodic-acid (TCS Biosciences Ltd, UK) and Schiff’s reagent (Merck, Germany). Description of the IF and IHC antibodies used can be found in the Supporting Information.
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bioRxiv - Microbiology 2022Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 µg/mL 2,6- Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
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bioRxiv - Bioengineering 2022Quote: ... Nitric acid 65%, N,N-dimethylformamide (DMF), sodium hydroxide (NaOH) and Ethylenediamine (EDA, ≥99 %) were purchased from Merck (Darmstadt, Germany). Dulbecco’s modified Eagle’s medium ...
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bioRxiv - Cell Biology 2022Quote: ... 0.05 M cacodylate buffer (using dimethylarsinic acid sodium salt trihydrate from Merck and HCl for pH adjustment to 6.4-7.4) at room temperature in a glass staining block ...
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bioRxiv - Microbiology 2023Quote: ... the pH of the TSB was adjusted with hydrochloric acid solution (1M and 6 M HCL; Merck KGaA, Darmstadt, Germany) immediately prior to the experiment ...
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bioRxiv - Plant Biology 2024Quote: ... Protein samples were separated on 12% non-reducing SDS-PAGE gel and cysteine-dimedone protein adducts visualised by western blot analysis using 1:10,000 diluted rabbit anti-cysteine sulfenic acid primary antibody (Merck Millipore) and 1:10,000 diluted horseradish peroxidase conjugated goat anti-rabbit IgG secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... we cloned the cytoplasmic domain of AChRα (mouse; amino acid 317-428) with or without mCherry into pGEX-4T1 (Cytiva 28-9545-49; Merck). GFP-SH3BP2 with or without the intrinsic disorder domain (amino acid 164-449 ...
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bioRxiv - Immunology 2024Quote: ... for 25 min in the dark at RT followed by a second stage blocking using 1M sulphuric acid (Merck, 1.00731.1000). Optical density (OD ...
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bioRxiv - Cancer Biology 2024Quote: ... 50 μL of sample (total homogenate or HEPES soluble fraction) was lyophilized for 12 h and digested with 50 µL of Nitric Acid (HNO3) (65% Suprapur, Merck) overnight at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were treated with Picrosirius red (0.1% Direct Red 80 dye in saturated picric acid; Sigma-Aldrich, Merck, Darmstadt, Germany) for 60 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... germanica adults using an antisense LNA (locked nucleic acid) probe conjugated to Digoxigenin (DIG) at the 5’ and 3’ ends (5’-DIG-GGAGGTCCCCCAGACCGGCACAGACCGAA-DIG-3’, Merck). Ovaries were dissected under Ringer’s saline ...
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bioRxiv - Cell Biology 2024Quote: ... the media was switched to DMEM high glucose supplemented with 2.5% heat- inactivated FBS and 10 μM Retinoic Acid (Merck, R2625). On day 7 ...
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bioRxiv - Cancer Biology 2023Quote: ... DLD-1 cells expressing different EPHB1 wildtype or mutant versions and EFNB1 were mixed in suspension at a ratio of 1:3 and plated at a density of 130,000 cells/cm2 on coverslips coated with 2 mg/cm2 of 1-2 mg/mL laminin (Merck KGaA, Germany, USA) and incubated at 37ºC in 5% CO2 ...