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Citations for Merck :
701 - 750 of 2532 citations for 3' Chloro 3 3 chloro 5 fluorophenyl 4' fluoropropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... paraffin-embedded tissue sections were deparaffinized and rehydrated by standard methods. Thereafter, sections were incubated with monoclonal mouse anti-p53 (mutant, clone Ab-3 PAb240 catalogue no. OP29-200UG) (Merck, Darmstadt, Germany) diluted 1:100 in PBS at 4°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... IHC staining was performed using Ki67 and cleaved caspase 3 antibodies and the 3,3’-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride (Merck Millipore, Darmstadt, Germany) as described previously30.
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bioRxiv - Cancer Biology 2024Quote: ... colonies from MDA-MB-468 cells were fixed by replacing the medium with a solution of 3% trichloroacetic acid in water (Merck, #T6399-500G), rinsed twice with water ...
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bioRxiv - Microbiology 2023Quote: ... Samples were centrifuged (12,000 g – 10 min) and supernatants were passed through 3 kDa Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore, catalogue no. UFC500396) for 45 min at 12,000 g at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... the slides were rinsed three times with PBS and blocked for 1 hour at RT in blocking buffer made with 3% BSA (Merck Millipore, 0218072801) and 0.02% Triton X-100 (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... followed by 2 x 10 min in 100% ethanol) and subsequently incubated for 3 min in HMDS (Hexam-ethyldisilazane, Merck, Gillingham, UK) before quickly air drying the samples ...
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bioRxiv - Biochemistry 2023Quote: ... on the proteolysis of 8 μM aSyn was investigated in the absence and presence of a cylindrical 10 mm×3 mm Teflon stirring bar (Merck, ref. Z328650) or three 4.5 mm diameter glass beads (Zymo Research ...
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bioRxiv - Neuroscience 2023Quote: ... was dissolved in vehicle (Ringer solution; NaCl 140 mM, CaCl21.2 mM, KCl 3 mM and MgCl2 1 mM (Merck KGaA Darmstadt, Germany)) to a dilution of 5.5μg/μl ...
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bioRxiv - Neuroscience 2023Quote: The viability of SH-SY5Y cells after indicated treatments was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) assay ...
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bioRxiv - Cell Biology 2024Quote: ... ultrapure water was added and the suspension was ultrafiltered using an Amicon Ultra-0.5 3 kDa cut-off ultrafiltration tube (Merck Millipore, Massachusetts, USA). The ultrafiltrate was concentrated using a SpeedVac vacuum concentrator (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... MCAD activity was measured using 3-phenylpropionyl-CoA (custom synthesis; 0.3 mM) as substrate and ferrocenium hexafluorophosphate as electron acceptor (Merck, 388297; 1.5 mM), followed by detection of 3-phenylpropenonyl-CoA using UHPLC (Wanders ...
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bioRxiv - Molecular Biology 2024Quote: ... and 6 mM 2-mercaptoethanol] and then vortexed with an approximately equal volume of glass beads (212–300 mm) for 3 minutes (Merck KGaA, Germany). The homogenate was centrifuged at 12,000 g for 8 min at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mL of culture was sonicated for 3 s and 200 μL were loaded into a CellASIC® ONIX2 Y04C microfluidic plate (Merck Millipore) connected to the CellASIC® microfluidic pump (Merck Millipore) ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were removed from the samples by ultrafiltration at 3000 g and 30 min using Amicon Ultra filters (3 kDa cut-off, Merck, Darmstadt, Germany). Subsequently ...
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bioRxiv - Cancer Biology 2021Quote: ... Percentage of GFP- or mCherry-positive cells was measured every three days from day 3 to day 18 post-infection using Guava® easyCyte™ Flow Cytometer (Merck Millipore). The percentage of fluorescent cells in the population on each day was divided by their percentage on day three to calculate fold-change corresponding to the effect of a given sgRNA on cell proliferation.
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bioRxiv - Biochemistry 2020Quote: ... and the buffer was exchanged to buffer B without imidazole using 3 kDa Amicon® centrifugal filters (0.5 ml size) (both from Merck KGaA, Darmstadt, Germany).
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bioRxiv - Biophysics 2020Quote: ... at different concentrations (20 mM, 200 mM and 500 mM) and concentrated using centrifugal ultrafiltration devices (3 kDa cut-off) (Merck India Pvt. Ltd.). This was followed by Gel filtration chromatography using Superdex 200 Increase 10/300 GL column (GE Lifesciences ...
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bioRxiv - Biochemistry 2021Quote: ... 500 µL filter sterilized supernatant was concentrated to 100 µL using Amicon ultra centrifugal filter units with 3 000 Da cut-off (Merck Millipore, Cork Ireland) and washed three times in 10 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 mL of a UPLC-grade water:methanol (3:1, v/v) solution with 1:500 diluted 13C-and 15N-labeled amino acids standard mix (Sigma-Aldrich, Merck, Darmstadt, Germany) was added to the tubes ...
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bioRxiv - Neuroscience 2024Quote: ... The tissue sections from the in vivo experiment were incubated with a rabbit primary antibody for GluR2/3 (Merck Millipore; AB1506; 1:200) overnight and then with a secondary goat anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Plant Biology 2023Quote: ... The sections were then incubated for 1 h with 700 µL of a 10% (v/v) DMSO/3% (v/v) IGEPAL® CA-630 (Merck, Darmstadt, Germany) in MTSB ...
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bioRxiv - Systems Biology 2023Quote: ... the 2x 50 μl were pooled and then split in 3 x 33 μl which were each dialyzed against 50 mL of MilliQ H2O (Merck Millipore, Darmstadt, Germany) as described above ...
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bioRxiv - Cell Biology 2023Quote: ... The fixing solution was aspirated and wells washed 3 times in a washing buffer composed of DPBS containing 0.5% of BSA (Merck Life Science, Gillingham, UK). The washing buffer was left in the wells for 10 minutes following the last wash ...
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bioRxiv - Molecular Biology 2023Quote: ... Buffer of the eluted fractions containing 6xHis-HULla was replaced by buffer G (50 mM Tris pH 8, 200 mM NaCl) in Amicon® Ultra – 3 kD Centrifugal Filters (Merck KGaA, Germany) and resulting solution was injected in a heparin column ...
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bioRxiv - Cancer Biology 2024Quote: ... The following day the membrane was washed with T-PBS (0.1% tween) (15 min x 3 times) and appropriate secondary antibody: (i) anti-rabbit antibody conjugated with peroxidase enzyme (Merck Sigma, Burlington, MA) was dissolved in 5% BSA in T-PBS (0.1% tween ...
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bioRxiv - Microbiology 2024Quote: ... Samples were centrifuged (12,000 g for 10 min) and supernatants were passed through 3 kDa Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore, catalogue no. UFC500396) for 45 min at 12,000 g at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... to calculate the volume equivalent of 100 µg of protein before loading onto a 3 kDa molecular weight cutoff (MWCO) filter (Merck Millipore, Bayswater, Australia). Tryptic peptides were prepared using the FASP protocol as previously described (75) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... of 50 mM EDTA was added to the reaction mixture and unchelated radioactive copper ion was removed by centrifugation using Amicon® Ultra centrifugal filters (regenerated cellulose, 3 kDa) (Merck Millipore, Billericia, MA). The radioactivity of 64Cu complexed NOTA-POx was measured in gamma counter prior to micelle preparation.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HPLC purification of the (S,S)-MLN-4760 was based on the comparison with the commercially available compound (MLN-4760; MW: 428.31; Merck, CAS N° 305335-31-3). The isolation of the desired (S,S)-diastereoisomers of the respective fluorinated MLN-4760 derivatives was based on the assumption that the HPLC elution sequence of the diastereoisomers would be identical to that of the MLN-4760 lead compound.
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed 3 times in TBS before being probed with protein A-peroxidase (Merck, 1:50000 in 10% Skimmed Milk in TBS). The membrane was incubated with 1x LumiGLO® / 1x Peroxidase (CellSignal ...
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bioRxiv - Microbiology 2023Quote: Aliquots of 3-12 μl of cell-free supernatant or enzymatic reactions were spotted onto silica coated plates (Merck, TLC Silica gel 60 F254) and resolved in butanol:acetic acid:water (2:1:1) ...
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bioRxiv - Immunology 2024Quote: ... the wellbeing of the fish was followed at least once a day and fish exhibiting symptoms, signs of pain or discomfort were euthanised with an overdose of a Tricaine anaesthetic (Ethyl 3-aminobenzoate methanesulfonate, (Merck, Kenilworth, New Jersey, USA)) ...
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bioRxiv - Biochemistry 2022Quote: ... The two aqueous streams were supplied with the cell solution and with a 3 μM substrate solution containing lysis agents (0.7× BugBuster protein extraction reagent, Merck Millipore; 60 kU/mL rLysozyme, Novagen) in droplet assay buffer ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Birds from the anxiogenic group received 2.5 mg/kg of β-CCM (β-carboline-3-carboxylic acid-N-methylamide [FG 7142], Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) dissolved in DSMO (Dimethyl Sulfoxide suitable for HPLC ...
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bioRxiv - Microbiology 2023Quote: ... slick SSW and non-slick SSW from sites #1 – #3 (Fig. 1b) were fixed with 25 % glutardialdehyde (0.5 % final concentration, Sigma-Aldrich/Merck Life Science AB, Solna, Sweden) and stored at −80 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... immediately euthanised in a petri-dish filled with overdosed tricaine methanesulfonate (MS-222, ethyl 3-aminobenzoate methanesulfonate salt; Merck/Supelco CAT#A5040, 200 mg/L), and quickly imaged (see dedicated section) ...
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bioRxiv - Developmental Biology 2021Quote: ... hsp70:R2nlsG double transgenic 5 dpf larvae were soaked in 10 µM 4-hydroxytamoxifen (4-OHT, Merck, Taufkirchen, Germany) or the corresponding amount of vehicle control ethanol for 10 h ...
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bioRxiv - Cell Biology 2020Quote: ... and subsequently washed 4 times in Quencher solution (5 mM Trolox (Merck), 10 mM Na-Ascorbate (Merck)) ...
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bioRxiv - Neuroscience 2023Quote: ... and stained with 5 µM 4′,6-diamidino-2-phenylindole (DAPI) (Merck) in PBS if required ...
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bioRxiv - Microbiology 2020Quote: ... Samples were stored at 4°C overnight before desilicification with 4% suprapure hydrofluoric acid (Merck; incubation of approximately 5 hours). Afterwards ...
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bioRxiv - Molecular Biology 2022Quote: ... The beads were pre-bound with 4-5 micrograms of antibodies (H3K4me3 – Merck 07-473 ...
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bioRxiv - Immunology 2024Quote: ... cells were treated for 4 h with 5 mg/ml Brefeldin A (Merck, Darmstadt, Germany) after 140 h incubation time ...
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bioRxiv - Immunology 2020Quote: ... naïve T cells were freshly isolated and subsequently cultured for 4/5 days in RPMI (Merck), containing 10% Heat Inactivated FCS (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... which then were transferred into 4-5 fold bigger volume of cOmpleteTM Protease Inhibitor Cocktail (Roche, Merck) in PBS and gently mixed for 30 minutes to aid HA dilution ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μL lipid extract was injected on a LiChroCART 250–4 LiChrospher® Si 60 (5 μm) (Merck) maintained at 25 °C ...
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bioRxiv - Immunology 2021Quote: ... Frozen tissues were sectioned to a thickness of 5 μm and fixed with 4% paraformaldehyde (Merck, Kenilworth, NJ). Slides were incubated with 1% BSA in PBST for 1 h to block the non-specific antibody binding ...
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bioRxiv - Microbiology 2024Quote: ... Products were separated on a LiChroCART 125-4 RP-18 end-capped 5 µm column (Merck, Darmstadt, Germany) with a solvent system of methanol and phosphoric acid (0.1% ...
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bioRxiv - Molecular Biology 2022Quote: ... concentrated with an Amicon Ultra-4 centrifugal filter with a 5 kDa molecular weight cutoff (UFC8003, Merck Millipore), snap frozen and stored at -80 °C either directly (used for cryo-EM ...
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bioRxiv - Cell Biology 2023Quote: ... sections were fixed with 4% PFA and titrated to pH 5 using a buffer containing sodium acetate (Merck) and sodium tartrate-dehydrate (Merck) ...
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bioRxiv - Biochemistry 2024Quote: ... 500 μM of the protease inhibitor 4-(2-aminoethyl)-benzolsulfonyfluorid hydrochloride (BioChemica) and 5 U/mL-benzonase (Merck) were added (final concentrations) ...