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Citations for Merck :
651 - 682 of 682 citations for palmitic acid ethoxylated phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Samples were passed through a 0.2 µm nylon membrane prior to analysis with liquid chromatography as described previously 50. Separation for organic acid quantification (i.e. SCFAs and intermediate metabolites) was conducted on a LaChrom HPLC-System (Merck-Hitachi, Japan) using a SecurityGuard Cartridges Carbo-H (4 × 3.0 mm ...
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bioRxiv - Cell Biology 2024Quote: Treatment of flies with antioxidant Vitamin C was performed by feeding male flies with food containing 100mM Vitamin C (L-ascorbic acid; Merck – A4403) for the two days before dissection.
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bioRxiv - Microbiology 2024Quote: ... Samples for VFA determination were further fixed by adding 1% v/v of 4 M sulfuric acid (H2SO4, Merck Millipore, Germany). The concentrations of VFAs and sulfate were determined using a 930 Compact IC Flex 1 with a Metrosep A supp 7 −250/4.0 column coupled with an 887 Professional UV/VIS detector (Metrohm ...
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bioRxiv - Biochemistry 2024Quote: ... and E105 amino acids were mutated to Alanine using the Quick-change site-directed mutagenesis via KOD hot start DNA polymerase (Merck Millipore). For TON_0321 expression ...
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bioRxiv - Cancer Biology 2024Quote: ... cell culture media was changed to FCS-free high glucose DMEM containing 12.5 µM 13C16-labelled or unlabelled palmitate conjugated to 2% fatty acid-free bovine serum albumin (BSA, Merck Life Sciences). Palmitate was prepared in cell culture media from 100 mM ethanolic stocks of palmitic acid and conjugated to fatty acid-free BSA by incubating at 55°C in media for 2 h ...
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bioRxiv - Cell Biology 2024Quote: ... The digestion was quenched by lowering the pH with formic acid (Fisher) and the sample was desalted with a ZipTip C18 (Merck-Millipore) column ...
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bioRxiv - Cell Biology 2024Quote: ... MRS 2211 (2-[(2-chloro-5-nitrophenyl)azo]-5-hydroxy-6- methyl-3-[(phosphonooxy)methyl]-4-pyridinecarboxaldehyde disodium salt and MRS 2395 (2,2-dimethyl-propionic acid 3-(2-chloro-6-methylaminopurin-9- yl)-2-(2,2-dimethyl- propionyloxymethyl)-propyl ester) were obtained from Merck (Darmstadt, Germany).
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bioRxiv - Plant Biology 2021Quote: ... Extracted peptides were dried in a speed-vac and resuspended in (v/v) 2% acetonitrile/0.2% trifluoroacetic acid (Merck, catalog no. 302031). A total of four biological replicates for each sample type was submitted.
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bioRxiv - Synthetic Biology 2022Quote: ... All yeast transformations were plated on synthetic complete (SC) plates containing 1.7 g/L yeast nitrogen base without amino acids and ammonium sulphate (Sigma Aldrich, Merck Life Science), 1 g/L monosodium glutamate (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Protein transfer was then visualized using 0.1 % Ponceau S solution (w/v) in 5% acetic acid (Merck Life Sciences, Cat #: P7170-1L) and imaged ...
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bioRxiv - Synthetic Biology 2023Quote: ... VpALDH and SlAlaR were combined (each at 0.05 μM) with 1 U mL−1 D-amino acid oxidase (DAAO) from porcine kidney (Merck product no. A5222) and 2 U mL−1 catalase from bovine liver (Merck-MilliPoreSigma ...
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bioRxiv - Cancer Biology 2024Quote: ... colonies from MDA-MB-468 cells were fixed by replacing the medium with a solution of 3% trichloroacetic acid in water (Merck, #T6399-500G), rinsed twice with water ...
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bioRxiv - Molecular Biology 2024Quote: The frozen brain tissue samples were first thawed on ice and homogenized in 25 volumes (w/v) of ice-cold 0.1 M perchloric acid (Merck KGaA, Darmstadt, Germany) using an ultrasound homogenizer (Soniprep 150 ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were loaded onto the nanoACQUITY UPLC Trapping Column (Waters Corporation, Milford, MA, USA) using a mobile phase consisting of water containing 0.1% formic acid (Merck KGaA, Darmstadt, Germany). Subsequently ...
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bioRxiv - Developmental Biology 2022Quote: ... 32.8 mM 20:4 and 60 mM 22:6) and pipetted dropwise into N2 media containing 3 mM fatty acid-free BSA (Merck, Cat. #A8806) at 37°C with constant stirring to obtain final concentrations of 3 mM 18:0 ...
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bioRxiv - Molecular Biology 2024Quote: ... formic acid (FA, 56302), DL-dithiothreitol (DTT, D5545), iodoacetamide (IAA, I1149) and calcium chloride (CaCl2, A4689) were purchased from Merck (Darmstadt, Germany). Sequencing grade trypsin LC-MS grade was purchased from Promega (#V5111 ...
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bioRxiv - Cell Biology 2024Quote: ... resuspended in 50 µl of water and thoroughly mixed at 1:1 volumetric ratio with matrix solution (2,5-Dihydroxybenzoic acid) (Merck KGaA, Darmstadt, Germany). One µl of each sample was deposited onto the MALDI (Matrix Assisted Laser Desorption Ionization ...
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bioRxiv - Biochemistry 2024Quote: Mixing of lysozyme crystals (7×2 mm; grown by batch crystallisation) with 25 mM sulfanilic acid azochromotrop (SAA, Merck, lmax 505–510 nm), a highly absorbing red dye ...
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bioRxiv - Cell Biology 2023Quote: ... 200 μl of cell suspension was collected and mixed with 200 μl of ice-cold HBSS containing 5% fatty acid-free bovine serum albumin (BSA, Merck Millipore, 126575-10GM). To quench the fluorescence of non-internalized NBD-phospholipids ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 mL of a UPLC-grade water:methanol (3:1, v/v) solution with 1:500 diluted 13C-and 15N-labeled amino acids standard mix (Sigma-Aldrich, Merck, Darmstadt, Germany) was added to the tubes ...
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bioRxiv - Microbiology 2023Quote: ... The protocol was similar to the other shake flask cultivations using a starting OD of 20 but fully 13C labeled glycolic acid was used (Merck product no. 604011) for all 13C cultures and unlabeled glycolic acid with a natural isotopologe distribution for all natC cultures ...
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bioRxiv - Genetics 2022Quote: ... 5 µg SA8 was dissolved in 10 µL 10 mM hydrochloric acid and digested with 20 ng/µL pepsin (Merck, cat. No. 10108057001) at 37 °C for 6 h ...
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bioRxiv - Microbiology 2024Quote: For the carbon assays the minimal medium was supplemented with the RPMI-1640 amino acid solution (Merck, Cat. No. R7131; 50 mL/L) as nitrogen sources ...
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bioRxiv - Immunology 2024Quote: ... 1-26 amino acids) was amplified by PCR using corresponding primers (Supplementary table 1) and subcloned into pET-32a (+) plasmid (Merck Millipore, Darmstadt, Germany). After confirmed by sequencing ...
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bioRxiv - Plant Biology 2024Quote: ... the identification of metabolites was confirmed by comparison with authentic commercial standards acquired via MS1 and MS2 under the same chromatographic conditions: hulupinic acid (Merck KGaA, Darmstadt, Germany), α– and β-acids (n ...
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bioRxiv - Microbiology 2020Quote: ... The internal standard was 1 μL of methyl heneicosanoate (10 mg/mL) and Bacterial acid methyl ester (BAME) mix (Merck-Millipore, Burlington, MA, USA) was used to identify the each peak of fatty acids and analytical standards for each fatty acid were used for quantification.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Birds from the anxiogenic group received 2.5 mg/kg of β-CCM (β-carboline-3-carboxylic acid-N-methylamide [FG 7142], Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) dissolved in DSMO (Dimethyl Sulfoxide suitable for HPLC ...
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bioRxiv - Plant Biology 2024Quote: ... samples were acidified to pH 2 with formic acid and desalted with pipette tips packed with six layers of C18 Empore™ SPE Disks (Merck, cat. no.66883-U) and dried in a Speed Vac at 45 °C for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... TSC (10 g/L) and citric acid (10 g/L) solutions were prepared in deionized (DI) water (Millipore Milli-Q®, Merck Life Science BV, Belgium) and filtrated through a 0.2 µm pore-sized syringe filter (Sarstedt ...
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bioRxiv - Developmental Biology 2024Quote: ... T3+IOP (10-7M 3,3′,5-Triiodo-L-thyronine, Merck/Sigma-Aldrich CAT#T2877; 10-7M Iopanoic Acid, Merck/Sigma-Aldrich CAT#14131; in DMSO), 10-30µg/L CPF (Merck/Sigma-Aldrich CAT#45395 ...
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bioRxiv - Developmental Biology 2024Quote: ... MPI (10-5M Methimazole, Merck/Supelco CAT#M8506; 10-6M Potassium perchlorate KClO4, Merck/Sigma-Aldrich CAT#460494; 10-7M Iopanoic Acid, Merck/Sigma-Aldrich CAT#14131; in DMSO), T3+IOP (10-7M 3,3′,5-Triiodo-L-thyronine ...