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Citations for Merck :
651 - 700 of 1111 citations for PF 4 CXCL4 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing full-size protein were concentrated using Amicon®Ultra 4 mL centrifugal filters (Merck). After the addition of 10% glycerol ...
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bioRxiv - Plant Biology 2023Quote: ... while 4-chloro-7-nitrobenzoxadiazole (NBD-Cl, 97%, Sigma-Aldrich, Merck Group, St Louis, MO, USA) was applied at 270LgLha−1 of active ingredient ...
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bioRxiv - Microbiology 2023Quote: The cells in 96-well plates were fixed with 4% paraformaldehyde (PFA, Merck, Cat. no. P6148) in phosphate buffered saline (PBS) ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing full-size protein were concentrated using Amicon®Ultra 4 mL centrifugal filters (Merck). After the addition of 10% glycerol ...
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bioRxiv - Molecular Biology 2023Quote: ... Harvested DIP material was clarified (3000 × g, 10 mins and 4 °C) and sucrose (Merck, #84097) was added at a final concentration of 4 % ...
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bioRxiv - Cell Biology 2023Quote: The eluate was concentrated using an Amicon Ultra-4 concentrator (Merck Millipore, 10 kDa cut-off), and incubated overnight at 4°C with TEV and 3C proteases to remove the C-terminal StrepTagII and C-terminal 6xHis tag from the HAUS2 and HAUS1 subunits respectively ...
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bioRxiv - Neuroscience 2023Quote: ... Amicon Ultra-4 centrifugal filters with 100,000 Da molecular weight cutoff (Merck Millipore, Burlington, MA, USA) along with 0.22 µM Nalgene® syringe filter units (sterile ...
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bioRxiv - Microbiology 2023Quote: SDS gel electrophoresis was performed using pre-cast mPAGE™ 4-20% bis-tris gels (Merck) and 20 µl of PS extracts were mixed in Laemmli buffer and boiled at 95°C for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated with 4’,6-diamidino-2-phenylindole (DAPI; 1:10,000; Merck Millipore) and mounted on slides with Mowiol (Merck Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... Amicon Ultra-4 centrifugal filters with 100 kDa molecular weight cutoff (Merck Millipore, Burlington, MA, USA) along with 0.22 μM Nalgene® syringe filter units (sterile ...
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bioRxiv - Cancer Biology 2024Quote: ... counterstained with 5µg of 4’,6-diamidino-2-phenylindole (DAPI – 1:1) (Sigma/Merck, D9542-10mg) to eliminate dead cells before running through the flow cytometer ...
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bioRxiv - Biochemistry 2020Quote: ... cells were stimulated for the indicated periods of time with antibodies to human CD3 (7.5 μg/mL, Cat. number 217570, Merck; Jurkat cells) and EGF (50 ng/mL ...
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bioRxiv - Physiology 2019Quote: ... following which they were treated with media containing 20% human serum (fed or fasted) and 1 µM puromycin (Merck Millipore Limited) for a further 4 h ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... PAb directed against phospho-STAT2 (Tyr689) and MAb directed against human IFNα/β receptor chain 2 (MAB1155) were purchased from Merck-Millipore.
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bioRxiv - Biochemistry 2021Quote: ... These Wild Type or Mutant Type let-7a-5p plasmids were co□transfected into treated human chondrocytes cells along with NC mimics or KCNQ1OT1 mimic (Sigma□Aldrich; Merck KGaA) using Lipofectamine 6000 following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and the Human Cytokine/Chemokine Magnetic Bead Panel (Cat# HCYTMAG-60K-PX30) on a Luminex MAGPIX system (Merck Millipore, Billerica, MA). Conditioned media concentrations (pg/mL ...
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bioRxiv - Microbiology 2022Quote: ... Aliquots of them were analyzed with the MILLIPLEX human cytokine/chemokine magnetic bead panel (HCYTOMAG-60K, Merck Millipore, Burlington, MA, USA) using the Luminex 200 multiplexing instruments (40-012 ...
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bioRxiv - Molecular Biology 2020Quote: ... mt1 and mt2 were generated by inserting the ORF of human TruB1 and its mutants into the pET22b vector (Merck Millipore) at the NdeI and XhoI sites ...
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bioRxiv - Neuroscience 2021Quote: ... pSIH-mCherry-KDEL construct expressing lentiviruses were added to the suspension of motoneurons before plating on polyornithine-(PORN) and human merosin-coated (CC085, Merck-Millipore) plates ...
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bioRxiv - Microbiology 2021Quote: ... Cytokine quantification was performed using a Human Cytokine/Chemokine/Growth Factor Panel A 48-Plex Premixed Magnetic Bead Multiplex Assay (Merck Millipore), using the Luminex MAGPIX System in 96-well plate format ...
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bioRxiv - Neuroscience 2022Quote: Human Neural Stem Cells (hNSCs) derived from the NIH approved H9 (WA09) human embryonic stem cell line were purchased from Merck (Germany). The hNSCs were plated on matrigel-coated 100 mm petri dishes and maintained in culture with NSCs expansion media (KO DMEM/F12 ...
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bioRxiv - Cell Biology 2022Quote: ... human very-low-density and low-density lipoproteins (catalog no. 437647-5MG and 6 mg/ml, LP2-2MG, Merck Millipore, respectively) were purchased and stored according to the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2023Quote: ... or human post-mortem total protein/ synaptoneurosome samples were then mixed into equal volumes of 2X Laemmli buffer (Merck: S3401- 10VL) and boiled for 10 minutes at 98 °C ...
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bioRxiv - Immunology 2023Quote: ... and PBMCs (1 × 106 cells per well) were seeded in 96-well plates in RPMI supplemented with 1% PNS and 10% AB human serum (Merck, UK) and stimulated with SARS-CoV-2 specific peptides pools ...
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bioRxiv - Developmental Biology 2023Quote: ... healthy female cynomolgus monkeys were subjected to follicular stimulation by intramuscular injection of 20 IU of recombinant human follitropin alpha (rhFSH, Gonal F, Merck Serono) for 8 days ...
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bioRxiv - Developmental Biology 2023Quote: ... a micro-infusion pump (iPRECIO SMP-200, ALZET Osmotic Pumps) with 15 IU/kg human follicle-stimulating hormone (hFSH, Gonal-f; Merck Biopharma) was embedded subcutaneously under anaesthesia and injected 7 µL/h for 10 days ...
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bioRxiv - Immunology 2024Quote: ... Primary human T-cells were isolated from peripheral blood (NHS Blood & Transplant Service, UK) using Ficoll-PaqueTM (Merck Group, Darmstadt, Germany) density gradient centrifugation followed by positive selection using antibody-coated magnetic beads directed against either human CD4 or CD8 antigen (Miltenyi Biotechnology ...
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bioRxiv - Neuroscience 2024Quote: ... MACS-enriched from fresh PBMC were seeded at 0.5×106 cells/cm2 in RPMI plus 5% heat-inactivated human AB serum (Merck Life Sciences) and 100 U/ml penicillin-streptomycin and cultured with 50 ng/ml rhIL-4 (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: CM was collected from TIS MDA-MB-231 cells for chemokine and cytokine analyses using a Milliplex MAP human cytokine/chemokine magnetic bead panel (Merck Millipore) according to the manufacturer’s protocol.
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bioRxiv - Biophysics 2021Quote: ... Fractions containing STAR635P-conjugated mSAv were concentrated with 10 kDa Amicon®Ultra-4 centrifugal filters (Merck) and stored in 1x PBS supplemented with 50 % glycerol at -20 °C ...
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bioRxiv - Biophysics 2021Quote: ... Monomeric STAR635P-labeled mSAv-DNA was concentrated with 10 kDa Amicon®Ultra-4 centrifugal filters (Merck) and stored in 1x PBS supplemented with 50 % glycerol at -20 °C ...
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bioRxiv - Biophysics 2021Quote: ... Fractions containing STAR635P-conjugated mSAV were concentrated with 10 kDa Amicon®Ultra-4 centrifugal filters (Merck) and conjugated to trans-Cyclooctene-PEG3-Maleimide (TCO-PEG3-Maleimide ...
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bioRxiv - Cancer Biology 2021Quote: ... Organoids were washed once with ice-cold PBS and were fixed by 4% PFA (#1004965000, Merck Millipore) for 20 min at RT and stored in PBS at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Cells were fixed with 4% paraformaldehyde (Intron, Seongnam, Republic of Korea) and 100% methanol (Merck, Darmstadt, Germany). The SFTSV foci were detected using rabbit anti-SFTS NP antibody (Abclon ...
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bioRxiv - Neuroscience 2021Quote: ... then incubated overnight at 4°C with anti-KCC2 (1:200; Merck Life Sciences, Italy, #07-432) or anti-IGF-1R β (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Inhibition of the TLR-4 pathway was achieved by treating cells with 2 µM TAK-242 (Merck) for 6 hours before adding particles ...
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bioRxiv - Pathology 2021Quote: ... The proteins were cleaned using Amicon Ultra-4 Centrifugal Filter Devices (15 ml, 10 kD; Merck Millipore) to remove the elution buffer ...
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bioRxiv - Microbiology 2021Quote: Yeast cells were digested at 90°C for 4 hours in 65% (w/v) HNO3 (Suprapur, Merck). Mineralized samples were diluted in 0.5% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were separated by SDS–PAGE (4-20%) and blotted onto polyvinylidene fluoride membranes (Merck Millipore). Membranes were blocked for 1 h with Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2022Quote: ... Elutions containing VgrG5-CTD were desalted using Amicon Ultra-4 Centrifugal Filter Units (Merck Millipore Ltd., UFC801096) into 10 mM imidazole ...
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bioRxiv - Immunology 2019Quote: ... 5 μg of the following antibodies were used: H4ac (Histone 4 pan-acetyl, Merck Millipore #06-866) anti-H3K27ac (Abcam ab4729) ...
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bioRxiv - Microbiology 2021Quote: ... was cultured in high-glucose (4.5 g × ml−1) Dulbecco’s modified Eagle’s medium (DMEM) containing 4 mM stable glutamine (Merck) and supplemented with 6% inactivated fetal calf serum (iFCS ...
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bioRxiv - Neuroscience 2020Quote: ... samples were incubated overnight at 4°C with primary antibodies against puromycin (1:500, MABE343, Merck Millipore), βIII tubulin (1:500 ...
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bioRxiv - Physiology 2019Quote: ... 4 µl of the lipid samples were spotted on a HPTLC plate (Merck Silica gel 60 F254) and developed with chloroform/methanol/water/triethylamine 30:35:7:35 (v/v/v/v) ...
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bioRxiv - Neuroscience 2019Quote: The cells were washed once with DPBS and fixed for 15 minutes with 4% paraformaldehyde (Merck Millipore), followed by three rinses with DPBS ...
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bioRxiv - Genetics 2020Quote: The cells were washed once with DPBS and fixed for 15 minutes with 4% paraformaldehyde (Merck Millipore), followed by three rinses with DPBS ...
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bioRxiv - Cancer Biology 2021Quote: ... cultures were treated with 4 μM of the CDK4/6 inhibitor Palbociclib/PD-0332991 (Merck, PZ-0199) for 8 days ...
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bioRxiv - Immunology 2020Quote: ... the cells were further cultured for 4 days in the same conditions plus Dexamethasone (1 μM; MERCK). IDCs were equally generated in 4 days ...
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bioRxiv - Microbiology 2021Quote: ... and adjusted before inoculation to the desirable level (ranged from 2.6 to 6.5 ± 0.1) by adding 4 M NaOH (Merck) or 4 M HCL (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2022Quote: ... Staining was achieved by adding a solution of 4-Nitro blue tetrazolium chloride (NBT, 11383213001, Merck-SIGMA) and 5-Bromo-4-chloro-3-indolyl phosphate disodium salt (BCIP ...