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Citations for Merck :
651 - 700 of 2009 citations for 8 9 5 α CHOLESTEN 24 METHYLENE 3 β OL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... E12.5 whole-mount embryos were analysed for β-gal activity with X-gal (0.6 mg/ml; Merck Millipore, B4252) in PBS buffer containing 4 mM potassium ferricyanide ...
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bioRxiv - Microbiology 2019Quote: ... smegmatis MC2 155 were prepared as described above and mixed with 0.2 mU of commercial cellulase (1,4-β-glucanase) from Trichoderma reesii ATCC 26921 (Merck) in 50 mM citrate-phosphate buffer at pH 5 (6.5 mM citric acid ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained for SA-β-galactosidase activity overnight using a Cellular Senescence Assay kit (Merck Millipore, Massachusetts, USA) and imaged the next day in the motorised widefield microscope Cell Observer (Zeiss ...
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bioRxiv - Microbiology 2021Quote: ... Five lactose positive colonies were selected form the plate and β-glucuronidase activity was confirmed using Chromocult Agar (Merck).
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bioRxiv - Neuroscience 2023Quote: ... Cells were fixed on 4% PFA and senescence determination was made by measuring SA-β-gal activity (KAA02 Merck) and IF against the markers of senescence ...
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bioRxiv - Immunology 2021Quote: ... Cloning of the antigen library into the T7 phages was performed following manufacturer’s instructions (T7Select 10-3 cloning kit, catalog no. 70550-3, Merck-Millipore, Burlington, MA, USA).
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bioRxiv - Pharmacology and Toxicology 2023Quote: When the tumor sizes reached to 80 mm3 animals were treated with a PFKFB3 inhibitor 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one) (3PO) (Sigma-Aldrich, Merck, Overijse, Belgium). The animals received intraperitoneal (i.p. ...
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bioRxiv - Molecular Biology 2020Quote: ... and incubated for 16h at 4°C with 2µL α-H3K27me3 antibody (#07-449; Merck Millipore, Burlington, MA, USA) or α-H3K4-me3 antibody (Rabbit monoclonal antibody (#04–745 ...
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bioRxiv - Physiology 2022Quote: ... monoclonal anti-α-actinin (Sarcomeric) antibody produced in mouse (1:1000, Sigma-Aldrich, Merck, clone EA-53, Cat# A7811), at 4°C overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...
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bioRxiv - Biochemistry 2019Quote: Water was used after purification with a Milli Q-8 direct system (Merck 119 KGaA, Darmstadt, Germany). Stock solutions with a concentration of 1 mM of each monosaccharide (Glc ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... twice and incubated with 8% formaldehyde in 0.05 M sodium cacodylate buffer (Paraformaldehyd from Merck, Darmstadt, Germany) pH 7.4 for 2h at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Viral pellets were resuspended in culture medium supplemented with 8 μg/ml Polybrene (Merck, TR-1003-G) and 10 μM Y-27632 (Selleckchem ...
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bioRxiv - Cell Biology 2021Quote: The cells were grown in an 8-well chamber slide (Millicell EX slide, Merck Millipore, Burlington, MA) to early confluency ...
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bioRxiv - Cancer Biology 2022Quote: ... and 8 M Urea was added to a 10 kDa spin filter column (Microcon, Merck-Millipore, USA), on-filter reduced ...
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bioRxiv - Neuroscience 2022Quote: ... IP samples were resolved on 8%–12% SDS-PAGE and electroblotted onto Immobilon-P membranes (Merck Millipore) using a semidry transfer apparatus ...
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bioRxiv - Microbiology 2022Quote: ... of which 8 μL was spotted on a 20×10 cm HPTLC Silica gel 60 plates (Merck). When dried ...
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bioRxiv - Molecular Biology 2023Quote: Approximately 1 × 107 cells were lysed in 200 μL lysis buffer [50mM Tris pH 8 (Merck, T6066), 150mM NaCl (Merck ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were sub-cultured on days 4 and 8 of differentiation onto human fibronectin (Merck Millipore, #FC010) coated plates at a ratio of 1:4 ...
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bioRxiv - Cell Biology 2023Quote: HEK cells were grown in an 8-well chamber slide (Millicell EZ slide, Merck-Millipore, Burlington, MA) to early confluence ...
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bioRxiv - Developmental Biology 2021Quote: ... The tip epithelial cells were infected with the modified lentiviral vector for 24 h at 37°C in a single cell suspension in the SN medium containing 10 μM Y-27632 (Merck, 688000). After 24 h ...
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bioRxiv - Biophysics 2020Quote: ... DNA origami were incubated with a 10x molar excess of dSA for 30 min at 24°C and excessive dSA was removed using 100kDa Amicon®Ultra centrifugal filters (UFC210024, Merck). As a last step ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were grown in 24-well plates and treated with increasing concentrations of CaCl2 and NaH2PO4 · Na2HPO4 alone or in combination with PPi (Merck, #P8010) and/or phosphatase (Prospec ...
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bioRxiv - Cell Biology 2021Quote: ... EVs containing fractions from 24 h conditioned medium were concentrated using a 100 kDA filter (Amicon Ultra-15, Millipore, Merck, Irealnd) and total RNA was isolated as previously described ...
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bioRxiv - Cancer Biology 2022Quote: ... 50% confluent cells were plated and allowed to adhere for at least 24 hours before treatment with 1 μM 4OH-TAM (Merck, H7904) or with identical volume of EtOH for the indicated time ...
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bioRxiv - Molecular Biology 2020Quote: ... all cell lines were seeded in black-walled 96 well plates at a density of 1×104 cells/well or and were maintained in the dark after induction of optogenetic receptor expression through 24 hours incubation in serum-free medium containing 100ng/ml doxycycline hyclate (Merck, #D9891). Optical stimulation was performed at room temperature using either a custom-built Arduino-controlled LED array device using 470nm LEDs (Ballister et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The full-thickness (epidermis and dermis) RHS was developed in-house in a 24-well plate size insert (Millicell-PCF, Merck, Brazil), and it was fully differentiated after ten days ...
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bioRxiv - Evolutionary Biology 2022Quote: ... slices were incubated for 24-48h at 4°C with mouse anti-Neurophysin I (1:2000, Merck Millipore, marker for Oxytocin), rabbit anti-vasopressin (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were freshly grown for 20 to 24 h on plates in anaerobic jars supplemented with Anaerocult C sachets (Merck, USA) at 37°C ...
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bioRxiv - Pathology 2023Quote: ... blocked with normal serum for 1 h at 22–24°C and incubated overnight at 4°C with rabbit polyclonal anti-Lubricin antibody (1:500, MABT401, MERCK, DEU). The sections were then washed three times with PBS ...
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bioRxiv - Neuroscience 2019Quote: ... After washing (5x 3 min) with deionized water (“MilliQ”, Merck Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... Centrifugal filters (0.5 mL, 3 k) were purchased from Merck KGaA (Darmstadt ...
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bioRxiv - Plant Biology 2021Quote: ... brassicicola was maintained on malt medium (3% malt extract (Merck), 1.5% agar-agar (Roth) ...
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bioRxiv - Plant Biology 2021Quote: ... and half-strength phosphatase inhibitor cocktail 2 and 3 (Merck). Samples were clarified twice by centrifugation at 14 000 g ...
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bioRxiv - Microbiology 2020Quote: ... cells were treated with MG132 overnight (ON, 3 μM, Merck). Cells were lysed in ice-cold RIPA buffer (150mM NaCl ...
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bioRxiv - Genomics 2020Quote: 3 commercially available control DNA samples were purchased from Merck Millipore (Burlington ...
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bioRxiv - Microbiology 2019Quote: ... and 25 U/mL of benzonase (Merck-Millipore, 70664-3)] ...
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bioRxiv - Cancer Biology 2020Quote: ... Endogenous peroxidases were blocked with 3% (v/v) H2O2 (Merck) in methanol (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... concentrated to ∼250 μL (Merck, Amicon, 3 kDa cut-off), were combined with 250 uL lysis buffer (1% CHAPS ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then fixed with 3% glutaraldehyde (Merck, Germany) in sodium phosphate buffer (0.1 M ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmid transfections were performed with 3 μl GeneJuice (Merck Millipore), 0.5 μg plasmid DNA in 200 μl Opti-MEM (Life Technologies) ...
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bioRxiv - Genetics 2021Quote: ... 33 mM 1,2-diheptanoylsn-glycero-3-phosphocholine (DHPC by Merck) and 1 % w/v sodium deoxycholate (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... using 3 kDa MW cut-off filter units (Merck Millipore). In order to detect and quantify the amount of NGF ...
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bioRxiv - Biochemistry 2022Quote: ... 3 mg/mL (∼40 µM) using Amicon 10K concentrators (Merck) and stored at -80°C till use.
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bioRxiv - Biophysics 2022Quote: ... We used Amicron ultracel-3 centrifugal filters (Merck Millipore Ltd) with 3-kDa molecular weight cut-off ...
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bioRxiv - Genomics 2022Quote: ... cells were passaged 1:3 using Accutase (Merck Millipore, #SCR005) in a single-cell suspension and seeded at 50000 cells/cm2 on 20 μg/ml laminin-coated 6-well plate ...
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bioRxiv - Cell Biology 2023Quote: ... Tissue slides were blocked with a 3% BSA (Merck, A7906) and 10% Donkey serum (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... peroxidase quench with 3% hydrogen peroxide (Merck, New Jersey, USA), protein block with Dako protein block solution (Dako ...
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bioRxiv - Developmental Biology 2024Quote: ... the TRPC3 inhibitor Pyr3 (Merck, 3 μM, 15-min incubation); or the TRPC6 inhibitor SAR-7334 (Selleck ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic acids were labelled with 3 µM DAPI (Merck, Belgium) for 20 minutes ...