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Citations for Merck :
651 - 700 of 3594 citations for 7 chloro 5 methylpyrazolo 1 5 A pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and were then blocked with PBS containing 2.5%, bovine serum albumin (BSA) (A1391-0250; Applichem, Germany) and 0.1 % Tween20 (9005-64-5; Merck, Germany) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... neurons were permeabilized and blocked with PBS containing 2.5%, bovine serum albumin (BSA) (A1391-0250; Applichem, Germany) and 0.1 % Tween20 (9005-64-5; Merck, Germany) for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... Tumors and lungs were paraffin-embedded and consecutive 5-μm sections were stained with hematoxylin-eosin purchased from Merck.
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miR-206 inhibits estrogen-induced proliferation and invasion of ER-α36 positive gastric cancer cellsbioRxiv - Cancer Biology 2021Quote: ... The PVDF membrane was sealed with 5% skim milk powder in the PBS containing tween (Sigma-Aldrich; Merck KGaA) for 3 h ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was clarified by centrifugation (500g, 5 min) and filtration (0.45 μm, Millex-HV, Merck Millipore (Burlington, MA)) ...
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bioRxiv - Biochemistry 2022Quote: ... diluted 1:1 with dilution buffer (50 mM Hepes pH7.5, 150 mM NaCl, 5 mM MgCl2, 0.625 U/ul Benzonase (Merck, 70746)) ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were lysed in lysis buffer (30 mM HEPES pH 7.5, 400 mM NaCl, 20 mM imidazole, 5% glycerol, 125 U/ml benzonase (Merck), 1 mM MgCl2 and complete protease inhibitor cocktail [Roche] ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were lysed in lysis buffer (50 mM HEPES pH 7.5, 150 mM NaCl, 5% glycerol, 125 U/ml benzonase (Merck), 1 mM MgCl2 and complete protease inhibitor cocktail [Roche] ...
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bioRxiv - Cell Biology 2022Quote: ... the nuclei were visualized with Hoechst 33258 (Merck, B2883, 2 µg/ml in H2O at RT for 5 min).
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Developmental Biology 2021Quote: ... hsp70:R2nlsG double transgenic 5 dpf larvae were soaked in 10 µM 4-hydroxytamoxifen (4-OHT, Merck, Taufkirchen, Germany) or the corresponding amount of vehicle control ethanol for 10 h ...
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bioRxiv - Systems Biology 2020Quote: The samples were analyzed using a high-resolution mass spectrometer in Orbitrap Q Exactive Plus (Thermo) equipped with a SeQuant ZIC-pHILIC column (150 mm x 2.1 mm x 5-micron packing, Merck) and a ZIC-pHILIC guard column (20 mm x 2.1 mm x 5-micron packing ...
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bioRxiv - Microbiology 2020Quote: ... The HPLC column RP18e Purospher-STAR (Hibar) (250 × 4.6 mm; 5 μm) was used for chemical fingerprinting (E. Merck) and Eclipse 5 μm column (9.4 × 250 mm ...
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bioRxiv - Molecular Biology 2022Quote: ... CAS-No: 7647-14-5) and ultrapure water from a Milli-Q system were supplied from Merck (Darmstadt, Germany).
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bioRxiv - Microbiology 2022Quote: ... 5°6.7’ E) in September and October 2017 by filtering 2 L of seawater through PVDF membrane filters (Merck Millipore ...
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bioRxiv - Systems Biology 2022Quote: ... of dimensions 150 mm x 2.1 mm x 5-micron packing and in sequence with a ZIC-pHILIC guard column (Merck) of dimensions 20 mm x 2.1 mm x 5-micron packing with the LC-MS/MS settings and calibrations as described previously (Iyer et al. ...
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bioRxiv - Genetics 2022Quote: ... The tissue sections were then dried for 5 min at 37°C and mounted with 85% glycerol (Merck, 104094) and a coverslip ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The HPLC setup consisted of a ZIC-pHILIC column (SeQuant, 150 mm x 2.1 mm; Merck KGaA, 5 mm), with a ZIC-pHILIC guard column (SeQuant ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were then permeabilized in blocking solution containing 5% normal goat serum (NGS, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) in 0.5% Triton for 1 hour at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... and 5 µl aliquots of the supernatants were applied to polyethyleneimine (PEI) cellulose thin layer chromatography plates (Merck, Sigma), resolved with 1.5 M KH2PO4 (pH 3.4 ...
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bioRxiv - Microbiology 2019Quote: ... and two zwitterionic functional groups – sulfoalkylbetaine bonded to (IV.) polymer support (ZIC-pHILIC, 100 × 4.6 mm, 5 µm; Merck) or (V. ...
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bioRxiv - Microbiology 2021Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Biochemistry 2020Quote: ... The bacterial pellet was suspended in 5 mL lysis buffer (BugBuster Protein Extraction Reagent, Merck Millipore, UK; containing DNAse) and incubated at room temperature for 30 mins ...
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bioRxiv - Pathology 2021Quote: ... hippocampal extracts prepared as described above were boiled for 5 min in 50 mM Tris-HCl pH 8.0 containing 12% Glicerol (Merck), 4% SDS ...
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α-catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity-dependencebioRxiv - Cell Biology 2021Quote: ... control cells were generated using the SHC202 -MISSION® TRC2 pLKO.5-puro Non-Mammalian shRNA Control Plasmid (Merck). After infection ...
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bioRxiv - Microbiology 2021Quote: ... USA) at a multiplicity of infection (MOI) of 5–10 in 1.5 mL Ex-Cell 405 medium (Merck, Germany), and the infected cells were incubated at 26 °C ...
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bioRxiv - Microbiology 2020Quote: ... The homogenate was centrifuged at 10,000× g for 5 min and the supernatant was filtered through a 0.45 μm filter (Merck Millipore) to remove large particles ...
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bioRxiv - Immunology 2020Quote: ... conditional knock out) allows deletion of Csf1r following treatment with the tetracycline analog doxycyclinehyclate (2μg/ml in 5% sucrose water; Merck) causing CSF1R expressing macrophage populations to be depleted ...
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bioRxiv - Microbiology 2020Quote: ... live cell fluorescence microscopy was performed as described before (85) with minor modification using 5 μg/ml Hoechst (Merck) to stain the nuclei ...
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bioRxiv - Immunology 2022Quote: Lungs were cut into 5 mm3 cubes and digested in 12 ml of collagenase I (2.4 mg/ml, Merck) and DNase I (1 mg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... Extracted metabolites were separated on a SeQuant ZIC-pHILIC column (2.1 × 150 mm, 5 μm bead size, Merck Millipore). Flow rate was set to 0.2 mL/min ...
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bioRxiv - Cell Biology 2022Quote: ... cells were permeabilized with 0.1% Triton X-100 for 3 minutes and then blocked with 5 or 10% bovine serum albumin (BSA, Merck) in PBS for 20 minutes ...
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bioRxiv - Immunology 2022Quote: ... approximately 5×105 Calu-3 cells were pre-treated with kp7-6 (100 ug/mL, CD95/CD95L antagonist, Merck) for 2 hour and then infected with SARS-CoV-2 at an MOI of 0.2 ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were cultured for at least 5 passages in DMEM containing either L- arginine and L-lysine (Merck) or L-arginine [13C6] and L-lysine [2H4] (Cambridge Isotope Laboratories).
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bioRxiv - Biochemistry 2023Quote: ... 400 μg of AtLEGβ or papain were inhibited with 0.5 mM or 5 mM S-methyl methanethiosulfonate (MMTS, Merck), respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatin was enzymatically sheared for 5 min at 37°C with 0.2 mU/μL Micrococcal nuclease (MNase) (#N3755, Merck), and stopped with MNase stop buffer (110 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... CD3+ T cells were cultured in TexMACS Medium (Miltenyi Biotech) supplemented with 5% heat-inactivated human AB serum (Merck), 100 units/mL penicillin (Merck ...
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bioRxiv - Microbiology 2023Quote: ... and SAM_Seq_Ex8_RV (5’-CTT CTT ATT GCC TCC TCT GGC ACA GC) together with KOD Hot Start DNA Polymerase (Merck) or KAPA HiFi HotStart ReadyMix ...
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bioRxiv - Cancer Biology 2023Quote: ... resuspended in 20-100 µl 70% acetonitrile in water and run on a ThermoFisher Q-Exactive Orbitrap mass spectrometer equipped with Zic-pHILIC column (150×2.1 mm, 5 mm; Merck). A volume of 5 µl was injected and metabolites were monitored in full-scan ...
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bioRxiv - Molecular Biology 2023Quote: ... The formula was build based on a twofold serious dilution of glycerol (>/= 99% purification, Merck, cat # 56-81-5). The glycerol density (microgram glycerol/mg hyphal weight ...
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bioRxiv - Microbiology 2023Quote: ... Single colonies from those plates were inoculated in 5 mL of tryptic soy broth (TSB; Merck KGaA, Darmstadt, Germany) and incubated for 16 - 18 h at 37°C with shaking at 200 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... LC-MS analysis was performed with a ZIC-pHILIC chromatographic column (5 µm, 2.1 × 150 mm, Merck, Darmstadt, Germany). Full scan positive and negative ionization modes with a resolution of 70.000 (FWHM ...
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bioRxiv - Immunology 2023Quote: Cells were infected separately with five different lentiviral transduction particles (at MOI = 5) containing five different shRNA species (Merck) specific for the mouse Flot2 gene (NM_008028 ...
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bioRxiv - Biophysics 2023Quote: ... The onion cells were prepared by dissecting a 5 mm x 5 mm section of the abaxial epidermis from a brown onion and mounting the membrane (5 mm x 5 mm) onto a #1.5 coverslip with 100 µl of neat Lugol’s iodine solution (62650; Merck, Germany) to produce a stained specimen.
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bioRxiv - Biochemistry 2023Quote: ... The proteins were reduced utilizing a 5 mM solution of tris-2-carboxyethyl-phosphine (TCEP) (Merck KGaA, Darmstadt, Germany) at a temperature of 60 °C for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: Protein solutions were first diluted with 50 mM ammonium bicarbonate (ABC) and reduced with 5 mM dithiothreitol (DTT, Merck) at 60 °C for 45 min ...
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bioRxiv - Genomics 2024Quote: ... media was changed for 500 µL of DMEM +5% FBS +4 μg/mL of polybrene (Merck TR-1003-G). Lentiviruses were diluted to the desired MOI in 500 µL of DMEM +5% FBS and slowly added to each well ...
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bioRxiv - Microbiology 2024Quote: ... Chromatography was performed on an Agilent Infinity II 1290 HPLC system using a SeQuant ZIC-pHILIC column (150 × 2.1 mm, 5 μm particle size, peek coated, Merck) connected to a guard column of similar specificity (20 × 2.1 mm ...
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bioRxiv - Biophysics 2023Quote: ... 20 µL 5% w/v solution of polyvinyl alcohol (PVA; fully hydrolyzed, MW = 145000 Da; Merck Group, Darmstadt, Germany) in water with 50 mM sucrose was spread onto a 2 cm by 5 cm area corresponding to the dimensions of a rectangular ...
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bioRxiv - Microbiology 2023Quote: ... Chromatographic separation was performed on a SeQuant ZIC-HILIC column (5 µm, 200 Å, 150 × 2.1 mm, Merck, Germany) equipped with a SeQuant ZIC-HILIC guard column (20 × 2.1 mm ...