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Citations for Merck :
651 - 700 of 2050 citations for 6 Chlorospiro chroman 2 4' piperidin 4 one hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... and soluble protein was clarified by centrifugation (50,000×g, 1 h, 4°C) and subsequently passed through a 0.22-μm filter (Merck Millipore).
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bioRxiv - Cancer Biology 2021Quote: Cells grown on coverslips were washed with PBS and incubated 15 min in 4% formaldehyde (formalin solution buffered at pH 6.8, Merck). Cells were washed once with 50 mM ammonium chloride (Carl Roth ...
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bioRxiv - Biochemistry 2021Quote: ... cells were trypsinized and seeded for selection in complete media supplemented with 4 μg/mL puromycin (Calbiochem, Merck Millipore). All cell lines were passaged 3 times before use and kept in culture supplemented with 2 μg/mL puromycin and/or stored in liquid nitrogen.
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bioRxiv - Cell Biology 2021Quote: ... and then equal volumes were concentrated 50-fold using Amicon Ultra-4 centrifugal filter units (Merck Millipore, Watford, U.K.). For analysis of cell-associated proteins ...
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bioRxiv - Microbiology 2021Quote: Cells grown on glass coverslips were fixed in 4% PFA for 30 min at RT and permeabilized with 0.2% Triton X-100 (Sigma/Merck) in PBS for 10 min at RT ...
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bioRxiv - Plant Biology 2021Quote: ... previously equilibrated with 50 mM HEPES buffer (pH 7.0) and concentrated with an Amicon Ultra-4 10K centrifugal filter (Merck).
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bioRxiv - Microbiology 2019Quote: ... The mixture was then centrifuged at 15,000 g at 4 °C and the supernatant was filtered with a 0.22 μm filter (Merck Millipore). A Ni-NTA column was used to purify the recombinant GB1-VpmR-S1 on an AKTA system (GE) ...
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bioRxiv - Bioengineering 2022Quote: ... samples were fixed in a solution of 4 % (v/v) paraformaldehyde supplemented with 1 % (v/v) glutaraldehyde (Merck, USA) overnight at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 108 - 109 plaque-forming units (PFU) mL-1 in tryptone soya broth (TSB, Oxoid) or in quarter-strength (1/4) Ringer’s buffer (Merck) unless stated otherwise ...
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bioRxiv - Physiology 2022Quote: ... containing 150 μL 4°C DMEM (Dulbecco’s modified Eagle’s medium with high glucose and 25mM HEPES from Sigma Aldrich (Merck), UK ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were centrifuged (16000 g, 20 min, 4°C) and filtered using Ultrafree Centrifugal Filter Units (Merck Millipore, USA).
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bioRxiv - Physiology 2020Quote: ... Membranes were probed overnight at 4°C with anti-phosphotyrosine antibody (1/1000, 05-321, clone 4G10, Merck Millipore) in blocking solution ...
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bioRxiv - Synthetic Biology 2021Quote: ... VLP fractions were pooled and concentrated using 100 kDa MW cut-off centrifugal filters (Amicon Ultra 4 ml, Merck). Protein concentrations were measured using the linearised Bradford method54 with Pierce Coomassie Protein Assay Kit (Thermo Scientific).
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bioRxiv - Plant Biology 2019Quote: ... and cell debris were removed by centrifugation (2x 30 min, 20000 g, 4 °C) and filtering through 0.45 µm syringe filters (Merck).
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bioRxiv - Genomics 2022Quote: ... Total chromatin was pre-cleared for 1h at 4°C with rotation using protein A sepharose beads (P9424, Merck). 3 µg of anti-histone H3 (Abcam ...
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bioRxiv - Immunology 2020Quote: ... with 1μg/mL anti-CD3 and 1μg/mL anti-CD28 at 4°C at a density of 105 cells/m in starvation media (RPMI (Merck), containing 0.5% Heat Inactivated FCS (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The virus-containing lysate was purified via iodixanol density-gradient ultracentrifugation (290,000 × g, 120 min, at 4°C) and rebuffered in PBS using amicon spin columns (Merck). Particles in PBS were stored in aliquots at −80°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were collected at 20,000 x g for 40 min at 4°C and soluble proteins in the supernatant were sterile filtered 5µm PVDF Membrane (Merck).
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bioRxiv - Immunology 2021Quote: ... Eluted RBDQ498Y and ACE2 were then concentrated and buffer-exchanged to TBS pH 7.4 using 10 kDa and 50 kDa 4 mL Amicons (Merck), respectively ...
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bioRxiv - Biochemistry 2022Quote: ... aliquots of 4 mL of the culture were vacuum-filtrated using 0.45 μm pore size filters (HVLP02500, Merck Millipore). The filters were transferred to -20°C cold 40:40:20 acetonitrile:methanol:water for metabolite extraction ...
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bioRxiv - Immunology 2022Quote: ... The sections were incubated overnight at 4°C with the following primary antibodies: KRT8 (Merck Millipore, MABT329, 1:100), p-ERK (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2023Quote: ... 1c and Fig.3 were first fixed with 4% PFA solution for 30 minutes at RT and quenched with 0.1 M glycine (Merck) solution in PBS for 15 minutes at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were fixed on 4% PFA and senescence determination was made by measuring SA-β-gal activity (KAA02 Merck) and IF against the markers of senescence ...
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bioRxiv - Microbiology 2022Quote: ... To each tube 1 μL of Tris((1-benzyl-4-triazolyl)methyl)amine (TBTA) solution (2.5 mM in DMSO; Merck), 10 μL of Tetrakis(acetonitrile)copper(I ...
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bioRxiv - Molecular Biology 2024Quote: The cell pellet was fixed in a mixture of 4% formaldehyde and 0.1% glutaraldehyde in 0.1M HEPES (Sigma-Aldrich / Merck) for 1 h at RT ...
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bioRxiv - Microbiology 2024Quote: ... samples maintained at 4°C were eluted through a ZIC-pHILIC column (5 μm, polymeric, 150 by 4.6 mm; SeQuant, Merck) by mobile phase A (20 mM ammonium carbonate ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysate was then spun at 20,000 g for 20 min at 4°C and loaded to a HisTrap HP column (Merck). The protein was eluted with the buffer with 300 mM NaCl ...
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bioRxiv - Genomics 2024Quote: ... media was changed for 500 µL of DMEM +5% FBS +4 μg/mL of polybrene (Merck TR-1003-G). Lentiviruses were diluted to the desired MOI in 500 µL of DMEM +5% FBS and slowly added to each well ...
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bioRxiv - Cancer Biology 2023Quote: ... 4561086) and transferred at 4°C to either Immobilon-FL or Immunoblon-P (IPVH00010) polyvinylidene fluoride membranes (Merck, IPFL00010) (1.5 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were separated on a 4–15% gel by SDS-PAGE and transferred to PVDF-FL membranes (IPFL00010, Merck). Membranes were blocked with 5% non-fat milk in PBS and incubated with primary antibodies diluted in 5% non-fat milk in PBS+0.1%Tween-20 (PBS-T ...
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bioRxiv - Microbiology 2023Quote: ... 108 – 109 PFU (plaque-forming units) mL-1) in TSB (tryptone soya broth, Oxoid) or quarter-strength (1/4) Ringer’s buffer (Merck)) was used in this study ...
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bioRxiv - Cancer Biology 2023Quote: ... was added for 16-18 hr at 4°C before washes and the addition of anti-phospho H2A.X mAb (Merck, Kenilworth ...
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bioRxiv - Biophysics 2023Quote: ... Peak fractions were pooled and concentrated in an Amicon Ultra-4 Ultracell 30 kDa centrifugal filter (Merck-Millipore #UFC803024). Aliquots were snap frozen and stored at −80 °C ...
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bioRxiv - Biochemistry 2023Quote: ... an aliquot was thawed and diluted to 4 μM in 1xPBS supplemented with 0.01% NaN3 (Merck, Cat. No. 71290) and incubated at 37 °C under quiescent conditions for one week ...
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bioRxiv - Bioengineering 2023Quote: ... for 20 min at room temperature (RT) and permeabilised overnight at 4 °C in 0.5% Triton X-100 (Merck) solution in PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions between 15.7 mL to 19.2 mL in elution volume were concentrated with Amicon Ultra-4 Centrifugal Filter Unit (10 kDa cutoff, Merck) to ∼2 mg/mL (measured by Bradford method) ...
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bioRxiv - Biochemistry 2023Quote: ... concentration and buffer exchange to TE (10 mM Tris-HCl pH7.5 and 1 mM EDTA) with an Amicon Ultra-4 centrifuge filter unit42 (30 kDa cut-off, Merck). Concentration of all plasmids was measured by NanoDrop One (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 24 h at +4°C with primary antibodies (goat anti-Chat (1:200, Merck, AB144P), mouse anti-GFAP (1:300 ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was concentrated by ultrafiltration (4°C, 3,500 x g) using Amicon Ultra Centrifugal Filter Units 10K molecular weight cut-off (MWCO) (Merck). CT produced in E ...
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bioRxiv - Cell Biology 2023Quote: ... Calibration curves were acquired using defined amounts of the fluorescent product 4-methylumbelliferone sodium salt (#M1508, Sigma-Aldrich/Merck). Fluorescence of 4-methylumbelliferone was normalized to crystal violet intensity.
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bioRxiv - Developmental Biology 2023Quote: Axolotl embryos of genotype tgSceI(Mmu.Prrx1:TFPnls-T2A-ERT2-Cre-ERT2; Caggs:loxP-GFP -loxP-mCherry)Etnka were treated with 4-OHT (Merck H7904) as described in 48 to permanently label connective tissue cells with mCherry ...
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bioRxiv - Biochemistry 2024Quote: ... containing kinase assay buffer (final concentration: 40 mM HEPES, 120 mM NaCl, [pH 7.5] and 0.3% CHAPS, 4 mM MnCl2 (Merck, 1059270100), 10 mM DTT (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... 2.7 mL of each sample was transferred into an Amicon Ultra-4 3K (3000 MWCO) filtration tube (Merck Millipore) and centrifuged for 40 minutes at 6225 g ...
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bioRxiv - Physiology 2024Quote: ... Membrane was incubated overnight at 4°C with specific mouse monoclonal primary antibody anti-puromycin (Merck, MABE343, 1:1000) and after wash ...
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bioRxiv - Genetics 2024Quote: ... The harvested medium was then centrifuged at 1,300 rpm at 4 °C for 5 min to remove cells and then filtered by 0.45 μm filter (Merck) and the virus was collected and stored at -80 °C.
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bioRxiv - Cancer Biology 2021Quote: ... α6-integrin (Merck, monoclonal, MAB1378), Laminin V (Merck ...
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bioRxiv - Developmental Biology 2023Quote: ... 6% PEG 4000 (Merck-Schuchardt), pH 5.0 ...
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bioRxiv - Biochemistry 2023Quote: ... 5(6)-Carboxyfluorescein (CF) (Merck; 100 mM CF stock ...
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bioRxiv - Neuroscience 2020Quote: ... and one 11μM nylon filter (NY1102500, Merck Millipore). The filtrate was centrifuged at 1500 rcf for 15 minutes at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... TBTX inducible knockdown in the TBXT shRNA sOPTiKD hESC line was achieved using tetracycline hydrochloride (Merck Life Science) at 1μg/ml as described previously (Bertero et al. ...