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Citations for Merck :
601 - 650 of 2148 citations for Porcine Circovirus 2 PCV2 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... cells were counterstained with 4,6-diamidino-2-phenylindol (DAPI, Merck) and mounted onto microscope slides with Mowiol 4-88 solution ...
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bioRxiv - Cancer Biology 2024Quote: ... 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI, Merck cat# D9542) was added to a final concentration of 1 μg/ml to each sample and was used to identify viable cells for analysis ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 µg/mL Dox and 10 µM TMP (Merck, 92131) were used.
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bioRxiv - Cell Biology 2024Quote: ... Mouse anti-centrin-2 (1:500 IF; 20H5) from Merck, mouse anti-CEP152 (1:1000 WB and 1:2000 IF ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 mM MgCl2) and incubated with 50 U Benzonase (Merck) for 1 h at 4 °C on a rotating wheel ...
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bioRxiv - Biochemistry 2024Quote: RNA aliquots (2 μg) were treated with DNase (Merck, AMPD1) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... resuspended in 2 ml of 1XRIPA buffer (Merck, 20-188) with washed sonification beads ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant fraction was collected and incubated with S-protein beads (Merck) for 2 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were concentrated by ultrafiltration using Amicon Ultra centrifugal filters (Merck Millipore) with 50 kDa cut off ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein was concentrated using an Amicon 10 kD filter column (Merck UFC801008D) and purity tested by SDS-PAGE.
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bioRxiv - Molecular Biology 2021Quote: ... proteins were transferred from the gel to a PVDF membrane (Merck, IPFL00010) by semi-dry transfer with Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-Ubiquitinylated proteins,clone FK2 (cat# 04-263, Merck Millipore; 1:1000). Ponceau solution was prepared with Ponceau BS (cat# B6008 ...
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bioRxiv - Biophysics 2020Quote: ... polyclonal antibody against brain lipid binding protein (BLBP) (Millipore, Merck KGaA, Germany), mouse antibody against nestin (Millipore ...
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bioRxiv - Physiology 2020Quote: ... proteins were transferred to a PVDF membrane (Immobilon-P, Merck Millipore, IPVH00010) with 160 mA for 90 min ...
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bioRxiv - Neuroscience 2020Quote: ... the protein solution was filtered through a 100 kDa filter (MERCK, MRCFOR100). The filtrate was transferred to a low-protein binding tube and 20 equivalents of dopamine (final concentration ...
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bioRxiv - Biochemistry 2021Quote: ... All remaining protein constructs were cloned into the pET28b vector (Merck Millipore). Unless otherwise stated ...
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bioRxiv - Biophysics 2021Quote: ... Separated proteins were transferred onto a PVDF membrane (IPVH00010, Immobilon-P; Merck) and incubated for 2hr with blocking buffer containing 5% Skimmed milk (70166 ...
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bioRxiv - Plant Biology 2020Quote: ... Protein concentrations were measured using a Direct Detect® Infrared Spectrometer (Merck).
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bioRxiv - Microbiology 2020Quote: Infected insect cells were disrupted with CytoBuster™ protein extraction reagent (Merck) and clarified by centrifugation at 4,300 × g for 20m before column loading ...
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bioRxiv - Microbiology 2020Quote: ... Target proteins were visualized with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore).
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bioRxiv - Microbiology 2020Quote: ... GST bound protein was then cleaved using biotinylated thrombin (Merck Millipore; 69672) according to the manufacturers instructions overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred onto the methanol-activated µm PVDF membrane (Merck #ISEQ85R) using 1X transfer buffer (2.5 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: The Magna RNA-binding protein immunoprecipitation kit (Merck Millipore, Billerica, MA, USA) was applied for the assay ...
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bioRxiv - Biophysics 2020Quote: ... 100 μl of 10 μg/ml recombinant protein G (Merck, Darmstadt, Germany) was added and incubated for 30 minutes ...
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bioRxiv - Biophysics 2020Quote: ... The protein was filtered through a 0.22 μm Millex-GV filter (Merck) to remove potential aggregates ...
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bioRxiv - Cell Biology 2021Quote: Co-IP was performed using Protein G Immunoprecipitation Kit (Merck, Cat # IP50) as per the manufacturer's protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were concentrated using 30K Amicon Ultra Centrifugal Filters (Merck, Darmstadt, Germany), flash-frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were transferred to Immobilon-P PVDF membrane (Merck Millipore, MA, U.S.A). The membranes were incubated for 1h using 5% low-fat milk solution in 1X TBS (50 mM Tris-Cl ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were electrotransferred onto an Immobilon-fl polyvinylidene difluoride (PVDF) membrane (Merck) at 30 V for 1 hour followed by100 V for a further hour at 4°C in 1× NuPAGE Transfer Buffer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Following transfer of the proteins onto a PVDF membrane (Merck Milipore, ISEQ00010), the blots were saturated with 5% BSA in Tris-buffered saline (TBS ...
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bioRxiv - Immunology 2022Quote: ... Proteins were transferred to Immobilon-FL polyvinylidene difluoride (PVDF) membranes (Merck Millipore), which were subsequently blocked with 5% blotting grade blocker (cat# 170-6404 ...
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bioRxiv - Biochemistry 2022Quote: ... Selenomethionine-labelled proteins were expressed in Escherichia coli B834(DE3) cells (Merck). All other proteins were expressed in Escherichia coli BL21(DE3 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The resolved proteins were electrotransferred to an Immobilon-P membrane (Merck, IPVH00010). The membrane was probed with specific antibodies listed in Supplementary Table 3 ...
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bioRxiv - Neuroscience 2020Quote: ... Protein lysates were concentrated using Microcon-10kDa Centrifugal Filter Units (Merck Millipore), according to manufacturer’s instructions and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... The desalted protein was concentrated on an Amicon 10,000 MWCO (Merck, Germany) and filtered through a 0.22 µM membrane.
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bioRxiv - Molecular Biology 2021Quote: ... after which the proteins were transferred to Immobilon P membranes (Merck Millipore). After blocking the membrane with 5% skimmed milk in TBS-T buffer solution for 1 h at RT or overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... protein was concentrated using a centrifugation filter (3K, Amicon, Merck, Darmstadt, Germany) up to a final concentration of 5 mg/mL and stored at −80°C.
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to a PVDF membrane (Merck Millipore cat. No. IPFL00010) after which the membrane was blocked using Odyssey Blocking buffer (PBS ...
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bioRxiv - Biophysics 2021Quote: ... proteins were separated from salts and concentrated using Amicon Ultra 15 (Merck). Full length DnaK chaperone was purified by Ni2+-NTA column ...
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bioRxiv - Neuroscience 2020Quote: ... Thirty μl of PureProteome™ Protein A/G Mix Magnetic Beads (Merck) was washed in TBST and then incubated with 5 μl anti-HA antibody for 60 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were transferred onto a PVDF membrane (Immobilon-P, Merck Millipore) and the membranes were blocked with TBS containing 0.1% Tween 20 and 5% non-fat dry milk for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred to an Immobilon-PVDF membrane (Merck, Cat.N. IEVH00005) at 100V for 75 minutes in transfer buffer (20% methanol ...
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bioRxiv - Microbiology 2023Quote: ... followed by the transfer of protein onto the PVDF membrane (Merck Millipore) using a wet-tank blotting system (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein levels were detected using chemiluminescent Clarity Western ECL reagent (Merck; GERPN2236) and developed using ImageQuant LAS 4000.
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bioRxiv - Molecular Biology 2023Quote: ... Protein aggregates were removed by centrifugation (0.45 um filter plate, Merck, #MSHVN4550). From the soluble fraction the protein concentration was determined (BCA assay ...
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bioRxiv - Bioengineering 2023Quote: ... Wells were then blocked with protein block (3% BSA fraction V (Merck) or 10% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 μL of protein RIPA lysis buffer (The Merck Group, Darmstadt, Germany) with proteinase (Halt™ Protease ...
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bioRxiv - Biochemistry 2023Quote: ... The peak protein pool was concentrated by Amicon Ultra-4 (Merck, #UFC801024), loaded onto a 15–35% glycerol gradient in 5 mL of buffer H containing 0.1 M NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein aggregates were removed by centrifugation (0.45 um filter plate, Merck, #MSHVN4550). From the soluble fraction the protein concentration was determined (BCA assay ...
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bioRxiv - Cancer Biology 2023Quote: Immunoprecipitation was performed using Protein-A Sepharose from Staphylococcus aureus (Merck Millipore) and anti-TKS4 antibody (Lányi et al ...