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Citations for Merck :
601 - 650 of 711 citations for Polyoxymethylene Homopolymer 20% PTFE Fiber Filled Granule since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... the sections were treated with 10% methanol in PB for 20 min and then incubated for 1 h at RT in incubation buffer: 5% normal donkey serum (NDS: Merck) and 0.2% Triton X-100 (Merck ...
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bioRxiv - Cell Biology 2023Quote: ... An aliquot of 40 µL was incubated (1h at 70°C on a heating block) by addition of 20 µL of the trimethylsilylating (TMSi) reagent (chlortrimethylsilane [Merck KGaA ...
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bioRxiv - Plant Biology 2024Quote: ... were freshly isolated from the plates by scrapping the agar using water supplied with 0.002% Tween 20 added and then filtered through miracloth (Merck Millipore), centrifuged at 3.000 x g for 7 min ...
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bioRxiv - Plant Biology 2024Quote: ... Blots were blocked for 1 hours at 21°C or overnight at 4°C with 5% (w/v) skim milk in PBS-T (PBS tablets; Merck 524650, 0.1% Tween-20; Merck P1379). The membrane was incubated with 1:5000 anti-flagellin antibody (Taguchi et al ...
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bioRxiv - Synthetic Biology 2024Quote: ... Stimulation of the controls was performed 8 h after transfection with 20 ng/ml TNF-α (Merck, cat. no.: H8916). Cells were lysed 24 h later by addition of 100 μl lysis buffer (25 mM Tris/HCl ...
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bioRxiv - Biochemistry 2024Quote: ... for 1 h at room temperature and overnight with primary antibodies in incubating buffer (Tris-HCl, 50 mM, pH 7.5; NaCl, 200 mM; Tween-20, 0.1% and Bovine Serum Albumin (Merck, Ref A2153), 3% ...
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bioRxiv - Evolutionary Biology 2024Quote: ... slides were incubated in 60% isopropanol for 20 sec followed by the incubation in Oil Red O solution (Merck 1.02419.0250) for 10 mins ...
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bioRxiv - Genomics 2024Quote: ... 20 mL of pre-heated (65°C) modified Cetyltrimethylammonium bromide (CTAB) extraction buffer [2% CTAB (H-5882, Merck, Darmstadt, Gerrmany); 100 mM Tris-HCl (pH8.0) ...
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bioRxiv - Developmental Biology 2024Quote: ... The lysates containing 300-500 µg of protein were supplemented with 20 mM DTT and 250 units Benzonase (250 U/µl, purity grade I, Merck) and incubated at 37°C for 1h ...
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bioRxiv - Neuroscience 2024Quote: ... and 20 μg/mL BDNF (Miltenyi, #130-096-286) from d11 and 500 μM dibutyryl-cAMP (Sigma-Merck, #D0627-1G), 1 μM DAPT (Miltenyi ...
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Enabling Epitope Analysis of Glycoproteins via HDX-MS Using Antigens with Uniformed N-linked GlycansbioRxiv - Biochemistry 2024Quote: ... After nurturing the cells for 4-5 days in the Expi293™ Expression Medium with kifunensine at a concentration of 5 - 20 μM,9 the supernatants were gathered and filtered by a 0.22-µm filter device (Merck Millipore). The TALON® Superflow Metal Affinity Resin and HisTALON™ Buffer Set (Takara Bio ...
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bioRxiv - Cell Biology 2024Quote: ... followed by two rounds of selection alternating between BCDA medium containing the appropriate antibiotic (30 µg/mL hygromycin B [Carl Roth], 20 µg/mL G418 [Merck] ...
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bioRxiv - Synthetic Biology 2021Quote: ... Seed cultures from glycerol stocks were inoculated 20 hours overnight in 5 mL Invitrogen Luria Broth Base LB medium (ThermoFischer, USA) and supplemented with Kanamycin sulfate (Merck, Germany) (50 μg/mL) ...
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bioRxiv - Cell Biology 2020Quote: ... the samples containing 20 nmol phosphate were subjected to TLC on an HPTLC plate (Silica gel 60; Merck Millipore, MA, USA) in the solvent system hexane/diethyl ether/acetic acid (80:20:1 ...
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bioRxiv - Neuroscience 2020Quote: ... cryoprotected for 48 h in 20% sucrose at 4°C and then snap frozen in isopentane (2-methylbutane, Merck GmbH, Austria) for 3 min at −60°C ...
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bioRxiv - Neuroscience 2021Quote: ... 1 ml of extract was incubated overnight at 4°C with rotation with 20 µg/ml anti-FLAG M2 antibody (Merck, F3165) and 50 µl Pierce magnetic protein A/G beads (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 25 min) and alkylated (20 mM iodoacetamide, 30 min, room temperature) and samples concentrated with Amicon Ultra-4 columns (Merck-Millipore), After protein precipitation with methanol/chloroform ...
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bioRxiv - Plant Biology 2021Quote: Arabidopsis seeds were surface sterilized with 1 mL 70% (v/v) ethanol containing 0.05% (v/v) Tween 20 (Merck, Darmstadt, Germany) by shaking on a table incubator at 1400 rpm for 3 min ...
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of 20 to 30 μg of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Immobilion-P, Merck Millipore) according to standard protocols ...
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bioRxiv - Cell Biology 2022Quote: KELLY cells were grown on 20 x 14 cm dishes and treated for 14-16 hours with 100 µM of 4-Thiouridine (Merck, T4509). Cells (1.6 × 108 ...
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bioRxiv - Neuroscience 2022Quote: ... was either boiled with 4x SDS sample buffer (4% SDS, 40% glycerol, 20% ß-Mercaptoethanol in 250 mM Tris-HCl, Merck) at 95°C ...
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bioRxiv - Neuroscience 2022Quote: ... To fix the cells they were washed once with warm PBS and incubated for 20 min at 37°C with 4 % paraformaldehyde (Merck Millipore). After washing ...
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bioRxiv - Microbiology 2020Quote: ... peptides were loaded onto an in-house zwitterionic Zic-HILIC SPE cartridge containing 20 mg of Zic-HILIC particles (10 μm, 200 Å; Sequant/Merck). The flow-through was collected and re-circulated through the column an additional three times ...
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bioRxiv - Immunology 2020Quote: ... samples were centrifuged at 22000 x g for 20 minutes at 4 °C and the supernatant was filtered through Millex GV 220 nm filter (Merck-Millipore). For acid treatment acetic acid was used with 0.1 N final concentration ...
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bioRxiv - Genetics 2020Quote: ... of total protein suspended in loading buffer were separated by SDS-PAGE using 8% or 4-20% gradient gels and transferred onto PVDF membranes (Immobilon-P or Immobilon PSQ, Merck Millipore) for immunostaining ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were freshly grown for 20 to 24 h on plates in anaerobic jars supplemented with Anaerocult C sachets (Merck, USA) at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... the solution was incubated at 30 °C for 90 min and then derivatized with 20 µl of N-methyl-N-trimethylsilyltrifluoroacetamide with 1% trimethylchlorosilane (Merck, USA) at 70 °C for 30 min54 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell extracts of equal total protein (20 μg each) were separated by SDS-PAGE and transferred to Nylon membrane (Merck Millipore). The membranes were blocked with 5% nonfat milk in TBST washing buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Bioengineering 2023Quote: ... 3.5 μm particle size, 100 Å pore size) connected to a ZicHILIC guard column (20 × 2.1 mm, 5 μm particle size) (Merck KgAA) a constant flow rate of 0.3 ml/min with mobile phase A being 0.1 % Formic acid in 99:1 water:acetonitrile (Honeywell ...
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bioRxiv - Neuroscience 2022Quote: ... Unencapsulated molecules were removed by washing at least 4 times the LPs suspensions with PBS pH 7.4 for 20 min at 4°C and 5000 x g using an Amicon Ultra-4 concentrator with 100 kDa cutoff (Merck Millipore). The degree of encapsulation was calculated by subtracting from the total mass of the molecule to be encapsulated that present in the flow-through and was found to be higher than 75% in all LP preparations.
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bioRxiv - Synthetic Biology 2022Quote: ... This solution was incubated at 37°C for 3-4 h before being transferred to a 250 mL flask containing 20 mL of 425-600 μm glass beads (Merck G9268) and 30 mL of sterile MilliQ water ...
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bioRxiv - Cell Biology 2024Quote: ... of cell lysates by incubating lysates with 0.5-2.5 µg of antibodies and 20 µl of protein G–Sepharose (Merck, GE17-0618-01) at 4°C for 2 h ...
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bioRxiv - Cancer Biology 2024Quote: ... was followed by 5 minutes of rinsing with water and 20-30 seconds of Eosin solution (Merck, 115935, 100ml supplemented with one drop of acetic acid, Merck, 1.00063). For PAS staining ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 3.5 μm particle size, 100 Å pore size) connected to a ZicHILIC guard column (20 × 2.1 mm, 5 μm particle size) (Merck KgAA), with a constant flow rate of 0.3 ml/min ...
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bioRxiv - Cancer Biology 2024Quote: ... After 30 minutes of incubation 100 μL of 2X STOP buffer (NaCl 340 mM, EDTA 20 mM, EGTA 4 mM, 5% Digitonin, 100 μg/mL RNase A (Merck, R6513), 50 μg/mL Glycogen (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... 3.5 μm particle size, 100 Å pore size) connected to a ZicHILIC guard column (20 × 2.1 mm, 5 μm particle size) (Merck KgAA), with a constant flow rate of 0.3 ml min−1 ...
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bioRxiv - Cell Biology 2023Quote: ... Similar lysis buffer conditions were used for HA-based affinity purification of SHA-BAG2 in presence and absence with 20 μM of the ULK1 inhibitor MRT68921 (Merck, SML1644) (2 h prior HBSS treatment ...
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bioRxiv - Cancer Biology 2022Quote: ... and additional wells with 20% biotinylated PLL-g-PEG and FNIII(7-10) were supplemented with 1 µM staurosporine (Merck Millipore) to serve as a positive control for apoptosis ...
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bioRxiv - Microbiology 2022Quote: ... and separating gel were used for SDS-PAGE and as a molecular weight standard protein marker (97, 66, 43, 29, 20, 14kDa) (Merck, India) was used ...
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bioRxiv - Developmental Biology 2023Quote: ... healthy female cynomolgus monkeys were subjected to follicular stimulation by intramuscular injection of 20 IU of recombinant human follitropin alpha (rhFSH, Gonal F, Merck Serono) for 8 days ...
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bioRxiv - Immunology 2023Quote: ... A549 cell were incubated with lentiviral supernatants diluted 1:20 (v/v) in culture medium containing 10 µg/ml polybrene (Merck Millipore) and centrifuged at 805xg at 20 °C for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 0.1% Tween-20 (PBS-T) and blocked using PBS supplemented with 4% w/v skimmed milk powder (PBSM-4%; Merck Millipore) for 1h at room temperature (RT) ...
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bioRxiv - Microbiology 2024Quote: ... then layered over 5 ml of 70% Percoll in a 15 ml tube (Percoll Merck P4937, 10% 10x PBS, 20% RPMI) and centrifuged at 1450 rcf for 11 min with break set to 1 and accelerator to 3 ...
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bioRxiv - Cancer Biology 2024Quote: ... and non-specific binding was blocked with 5% milk powder or 5% bovine serum albumin (BSA) in tris-buffered saline containing 0.05% Tween-20 (Merck, Darmstadt, Germany). Blots were incubated overnight with mouse anti-gp130 (R&D systems ...
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bioRxiv - Cell Biology 2024Quote: The collected samples (1×105 cells) were separated by SDS-PAGE (SuperSep Ace, 5–20% (Wako) and transferred onto PVDF membranes (Immobilon®-P (Merck)) ...
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bioRxiv - Neuroscience 2024Quote: ... the elution of the biotinylated proteins was carried out boiling the beads in 25 μl of 3X protein loading buffer supplemented with 20 mM DTT and 2 mM Biotin (Merck, B4501) for 10 min at 95°C in gentle shaking ...
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bioRxiv - Neuroscience 2024Quote: ... and equal amounts of proteins were separated by SDS-PAGE (10% or 4-20% gradient gels) and transferred to Immobilon-P membranes (Merck-Millipore). After chemiluminescence detection ...
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bioRxiv - Neuroscience 2024Quote: ... An aliquot (40 µL) was incubated (70° C, 1h) in addition to 20 µL of trimethylsilylating (TMSi) reagent-comprising chlortrimethylsilane (Merck, Germany), 1.1.1.3.3.3-hexamethyldisilasane (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... DRG neurons were plated in 20 μg/mL PLL + 5 μg/mL Laminin coated glass surfaces in DMEM/F12 (Merck, D8437) supplemented with B27 (Gibco) ...
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bioRxiv - Biochemistry 2024Quote: ... 18 µL of a 20 µM solution of LmPDTN56C was mixed with 2 µL 100x SYPRO Orange (Merck kGaA, Darmstadt, Germany). The samples were then subjected to a temperature ramp spanning from 35 to 95 °C at 1 °C/min recording the fluorescent emission (Exc ...