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Citations for Merck :
601 - 650 of 1612 citations for Phosphatidylinositol N acetylglucosaminyltransferase subunit Q PIGQ Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The frozen cell pellets were incubated with 25 mM Tris buffer (pH 7.4) containing 0.05% n-dodecyl-β-D-maltoside (Merck, D4641) and 1 mM CaCl2 ...
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bioRxiv - Biochemistry 2021Quote: ... were constructed using standard genetic engineering techniques with a His6 tag at the N-terminus using the pET28a vector (Novagen, Merck). The recombinant CAHS1 protein ...
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bioRxiv - Biochemistry 2021Quote: ... varieornatus CAHS1 protein was constructed and cloned as a fusion protein with a hexahistidine (His6) tag at the N-terminus using a pET28a vector (Novagen, Merck). CAHS1-N (Met1-Ser96 ...
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bioRxiv - Biochemistry 2021Quote: ... Reactions were stopped at each time point using 4 N formic acid and analyzed by PEI-Cellulose-F TLC (Merck) developed in 0.4 M potassium phosphate buffer (pH 3.4) ...
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bioRxiv - Immunology 2021Quote: ... neuraminidase-specific inhibitor DANA was added to a final concentration of 100 μM (N-Acetyl-2,3-dehydro-2-deoxyneuraminic acid, Merck, USA). It was used for 48 h prior stimulus.
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bioRxiv - Plant Biology 2023Quote: ... the sequence of CBL1 was fused with an N-terminal 2xStrepII tag in the pET-24b vector (Merck, Darmstadt, Germany). Sequences of active CPK5 and CIPK26 were fused with an N-terminal 2xStrepII tag in the pIVEX-1.3WG vector (Biotechrabbit ...
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bioRxiv - Microbiology 2023Quote: ... 1 L of freshly 0.1 µm filter-sterilised seawater (n = 2) was filtered onto a 0.2 µm Sterivex (Merck Pty Ltd) filter and snap-frozen in liquid nitrogen ...
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bioRxiv - Immunology 2024Quote: ... and passaged two times on MDCK-II cells with FBS-deficient medium containing 1% penicillin/streptomycin and 1 µg/ml of N-tosyl-L-phenylalanine chloromethyl ketone-treated trypsin (TPCK-trypsin, Merck KGgA ...
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bioRxiv - Biochemistry 2023Quote: ... The residue was suspended in 10 µL of n-butanol saturated in water and chromatographed on silica 60 plates (20×20 cm, Merck). Plates were developed for 7-10 cm with isopropyl alcohol/ammonia (32%)/water (6:3:1 by volume) ...
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bioRxiv - Microbiology 2023Quote: ... LB plates were seeded with the different cultures and Whatman n° 1 filter discs (6 mm) were impregnated with 5 μl of 30% (v/v) H2O2 (Merck) as previously described [31] ...
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bioRxiv - Biophysics 2024Quote: The gene encoding human GRB2 in addition to a N-terminal His6 tag with a tobacco etch virus (TEV) protease cleavage site was constructed into the vector pET14b (Merck) for over-expression in the Escherichia coli BL21 (DE3 ...
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bioRxiv - Biochemistry 2024Quote: ... For the determination of the Ki inhibitory constant of macrocyclic peptide inhibitor MP5.4.3 against PT5 we used chromogenic substrate N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide (100 μM; Merck, Darmstadt, Germany) at final concentration of 100 μM ...
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bioRxiv - Neuroscience 2023Quote: ... after testing multiple antibodies (anti-NeuN rabbit Antibody, ABN78 & ABN78C3, Merck; anti-NeuN rabbit Antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used were mouse anti-EGFR antibody (#GR01; Merck Millipore), goat anti-EEA1 (#sc-6414 ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibody RNA-DNA hybrid (s9.6) antibody (Merck Millipore, #MABE1095, 3ul) or GFP (Thermo Fisher ...
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bioRxiv - Molecular Biology 2020Quote: ... were used at a 1/10000 dilution before reaction with Immobilon Forte Western HRP substrate (WBLUF0100, Merck Millipore).
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bioRxiv - Molecular Biology 2021Quote: ... StrepII-tagged proteins (RBBP6, symplekin and WDR33) were detected using streptavidin-HRP conjugate (Merck Millipore, cat. No. 18152) and Amershan ECL Detection Reagents (Cytiva ...
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bioRxiv - Bioengineering 2021Quote: ... Binding of biotinylated S1-RBD to coated VNAR-hFc was detected by streptavidin-HRP (Merck Millipore, 18-152). The signal intensities were collected for each primary VNAR-hFc antibody without competitor and compared to the signal when a competitor antibody was present ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-rabbit IgG HRP (AP182P, Merck Millipore) or donkey anti-mouse IgG HRP (AP192P ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed 3 times with PBS-T for 5 min and incubated with mouse-HRP (A4416; Merck) and rabbit-HRP (GENA9640V ...
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bioRxiv - Molecular Biology 2022Quote: ... Immunodetection was performed using a Luminata Forte Western HRP Substrate system (Merck Millipore, Burlington, USA, 61-0206-81) or a Chemi-Lumi One L system (Nacalai Tesque ...
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bioRxiv - Immunology 2023Quote: Immunoblotting was performed as described above and MIF-6xHis-biotin revealed using streptavidin-HRP-conjugate (Merck, Darmstadt, Germany), while MDL1-6xHis was revealed using a custom-made monoclonal primary antibody (1:10 dilution) ...
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bioRxiv - Microbiology 2023Quote: ... were used at a 1/10000 dilution before reaction with Immobilon Forte Western HRP substrate (WBLUF0100, Merck Millipore). Signals were acquired with Fusion FX (Vilber).
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bioRxiv - Developmental Biology 2021Quote: ... We used the following primary antibodies: rabbit antibody against Protein Kinase Cα diluted at 1:500 (Antibody Registry ID: AB_477345, Merck), rabbit antibody against Recoverin at 1:1000 (AB_2253622 ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated with the primary antibodies (see the list of antibodies in Table S2) and with an anti-rabbit secondary antibody (Merck), and visualized by chemiluminescence substrate (Merck ...
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bioRxiv - Biochemistry 2021Quote: FLAG-CAHS1 was constructed using standard genetic engineering techniques with an N-terminal FLAG using a pT7-FLAG-1 vector (Sigma-Aldrich, Merck). The bacterial cells of E ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... Resulting lipid films were dissolved in 1 mL of n-hexane containing a C19:0 as internal standard (1.03 μg mL−1, CAS number 1731-94-8, Merck, Darmstadt, Germany) with addition of 200 μL of a solution of potassium hydroxide (KOH ...
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bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
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bioRxiv - Neuroscience 2024Quote: ... IL-10 and TNF-α were quantified in the FN of NL and at 21dpi aged GFAP-IL6Tg and WT mice (n= 4-6/experimental group) using a Milliplex MAP Mouse High Sensitivity kit (#MHSTCMAG-70K; Merck Millipore), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... a cassette coding for tdTomato with an N-terminal nuclear localization sequence and lack of a stop codon and a puromycin cassette with an N-terminal self-cleaving T2A peptide were amplified by KOD polymerase (Merck, 71085) and subjected to Gibson assembly.
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bioRxiv - Genetics 2023Quote: Chemical extractions of single wasps were performed by immersing them in 50 µl HPLC-grade n-hexane (Merck, KGaA, Darmstadt, Germany) in 2 ml glass vials (Agilent Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... Purified Mpro with authentic N- and C-termini was concentrated by a 10 kDa MWCO Amicon Ultra 50 centrifugal filters (Merck Millipore) at 4 °C to ∼ 10 mg/ml ...
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bioRxiv - Biochemistry 2023Quote: The SUN domain sequence of human SUN1 (amino-acids 616-812) was fused to a TEV-cleavable N-terminal His6-GCN4 tag and cloned into a pRSF-Duet1 vector (Merck Millipore). The KASH domain sequence of human JAW1/LRMP (amino-acids 515-555 and 531-555 for KASH6 and KASH6 core ...
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The node of Ranvier influences the in vivo axonal transport of mitochondria and signalling endosomesbioRxiv - Neuroscience 2024Quote: ... with 7.5-10 μg of HCT in PBS in a volume of ∼3.5 µl using a 701 N Hamilton® syringe (Merck, 20,779) for TA ...
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bioRxiv - Plant Biology 2024Quote: The presence of T-DNA in transgenic plants was confirmed via extraction of genomic DNA and genotyping by PCR with the REDExtract-N-Amp™ Plant PCR-Kit (Merck, Darmstadt ...
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bioRxiv - Biochemistry 2024Quote: ... The activity assay was performed by incubating 0.5 nM PT5 with 100 μM chromogenic substrate N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide (100 μM; Merck, Darmstadt, Germany) and two-fold macrocyclic peptide dilutions ranging from 0.0005 to 2000 nM ...
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bioRxiv - Cancer Biology 2024Quote: PC4 cDNAs wild type or carrying the W89A mutation and with a N-terminal 6x His-tag sequence were cloned into a pET-Duet1 plasmid (Merck Millipore) and transformed into competent BL21 cells (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... Membrane was washed 3x5 minutes with 50mls PBS and HRP activity was detected using ECL according to the manufacturer’s instructions (Merck Life Science UK Limited Cat no GERPN3243) ...
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bioRxiv - Cell Biology 2023Quote: ... Detection by enhanced chemiluminescence was performed using the Immobilon Forte HRP Western substrate (ref WBLUF0500, Merck Millipore, Darmstadt, Germany) or SuperSignal West Femto Maximum Sensitivity Substrate (ref 34096 ...
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bioRxiv - Cancer Biology 2023Quote: ... Detection by enhanced chemiluminescence was performed using the Immobilon Forte HRP Western substrate (ref WBLUF0500, Merck Millipore, Darmstadt, Germany) or SuperSignal West Femto Maximum Sensitivity Substrate (ref 34096 ...
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bioRxiv - Neuroscience 2023Quote: ... after which the blot was developed with the ECL chemiluminescent reagent (WBKLS0500, Immobilon Western Chemiluminescent HRP Substrate, MERCK, USA). We obtained an intense band at ∼40kD and a very faint one at ∼32kD (Supplementary Figure 1(c) ...
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bioRxiv - Cell Biology 2023Quote: ... The immunoreactive bands were detected by using enhanced chemiluminescence (ECL) reagent (Immobilon Crescendo Western HRP substrate; Merck Millipore, DE). Band densities were quantified by densitometry using Image LabTM Version 6.0.1 2017 (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... antibodies (α-GluA2, Merck clone 6C4 ...
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bioRxiv - Immunology 2020Quote: ... antibodies in RPMI (Merck), containing 10% Heat Inactivated FCS (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies (NaV1.1 Merck, AB5204 ...
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bioRxiv - Neuroscience 2021Quote: ... primary antibody and with mouse monoclonal anti-map2 primary antibody (Merck Millipore, MAB3418). The secondary antibodies used were the anti-Rabbit Alexa Fluor 546 (Thermo fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies included: goat anti-mouse IgG antibody (Merck Millipore AP308P, (H+L) HRP conjugate ...
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bioRxiv - Microbiology 2024Quote: ... suis antibody (1:100) and a mouse anti-tubulin beta III antibody (Merck Millipore ...
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Synaptic inhibition in the accessory olfactory bulb regulates pheromone location learning and memorybioRxiv - Neuroscience 2024Quote: ... Primary antibody incubation (1:1000 ul of anti-Chicken NeuN antibody, ABN91, Merck in 7.5% NGS,2.5% BSA in 1% triton and 1 X PBS ...