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Citations for Merck :
601 - 650 of 6193 citations for N' 4 Dimethylamino phenyl methyl 5 methyl 1 2 oxazole 3 carbohydrazide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Acetylated histone 3 was detected using a polyclonal rabbit antibody (Merck, 06-599, 3260200, 1:500) and visualized using a donkey anti-rabbit Alexa Fluor 488 secondary antibody (Molecular Probes ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng G-CASE and 3 mL PEI solution (1 mg/mL) (Merck KGaA, Darmstadt, Germany). 100 µL transfected cells were seeded per well onto 96-well plates (Brand ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM DTT) using Amicon Ultra-15 3 K or 30 K centrifugation units (Merck Millipore). The phase separation assay was performed in the presence or absence of 10% polyethylene glycol 8000 (PEG-8000 ...
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bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
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bioRxiv - Bioengineering 2022Quote: ... gels were washed for around 2-4 h with PBS and incubated in 1 ml 1 μM Alexa Fluor 546 NHS ester (NHS-AF546, #A20002, Merck), overnight at room temperature on a slowly rotating shaker ...
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bioRxiv - Biochemistry 2020Quote: ... Total extract was clarified by 20 min centrifugation at 30,000 rcf at 4°C and the soluble fraction was loaded on affinity chromatography over 5 mL of NiNTA resin (Sigma-Aldrich Merck, Darmstad Germany). Resin was step-washed with 10 mL of buffer A supplemented with 10 ...
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bioRxiv - Microbiology 2024Quote: ... AGBs (4 mm) and glass beads (4 mm, Merck, Germany) were inoculated with P ...
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bioRxiv - Molecular Biology 2022Quote: ... rinsed with PBS and the proteins were visualized with Immobilon Western Chemiluminiscent HRP substrate (Merck/Millipore, used at 1:1:3 (H2O) dilution ...
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bioRxiv - Immunology 2020Quote: Survival was determined by assessing the fraction of vital cells at defined time points during culture by flow cytometric exclusion of 4’,6-Diamidin-2-phenylindol (DAPI) (Merck, Darmstadt, Germany) and Annexin V-APC (BD Biosciences ...
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bioRxiv - Biophysics 2020Quote: Upon deposition the samples were washed 2 times 15 minutes with PBS prior to overnight fixation with 4% paraformaldehyde (PFA, Merck, Darmstadt, Germany). Washing steps and incubation took place under agitation at 4 °C unless stated otherwise ...
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bioRxiv - Neuroscience 2023Quote: GNAO1+/G203R#2 cells at the stage of neuronal rosettes (30 day of differentiation) were fixed in 4% paraformaldehyde solution (Merck Life Sciences) for 15 min at room temperature and washed thrice with 1X PBS (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were washed in 1x PBS and subsequently mixed with 100 μL 4% SDS (Carl Roth GmbH, Karlsruhe, Germany) and 2% beta-Mercaptoethanol (Merck, Darmstadt, Germany) in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated under 365 nm UV-light for 15 min with 666 µg sulfosuccinimidyl 6-(4-azido-2-nitrophenylamino)hexanoate (sulfo-SANPAH, Sigma-Aldrich, Merck, 803332) diluted in 10 mL sterile dH2O ...
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bioRxiv - Biochemistry 2020Quote: ... Immobilon Chemiluminescent HRP substrate (Cat. n. IMGDV002, Merck Millipore, Watford, UK) was used for detection ...
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bioRxiv - Biophysics 2022Quote: ... N-hydroxy-succinimide ester (0.1%w/v, Merck Chemicals GmbH, Germany) was added to the oil solution to functionalize the phantoms with Alexa 488 ...
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bioRxiv - Microbiology 2023Quote: ... proteins were extracted in n-dodecyl-β-D-maltoside (DDM, Merck) 1% detergent ...
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bioRxiv - Immunology 2021Quote: ... Color was developed in the dark for 5-10 min before adding 50 µL of 2 M H2SO4 stop solution (Merck, Germany). Optical density was read at wavelength 450 nm using the microplate reader (SpectraMax ID3 ...
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bioRxiv - Molecular Biology 2024Quote: ... The nuclei pellet was washed once in 5 mL of Honda buffer then purified on a Percoll density gradient as follows: 2 mL of 75% Percoll (Merck, P7828) in Honda buffer topped with 2 mL of 40% Percoll in Honda buffer topped with the nuclei pellet resuspended in Honda buffer in a 15 mL tube ...
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bioRxiv - Developmental Biology 2023Quote: Cells were homogenized in 20 mM Tris-HCl buffer (pH 7.2, 0.2 mM EGTA, 5 mM β-mercaptoethanol, 2% (v/v) antiprotease cocktail (Merck KGaA, Germany), 1 mM PMSF ...
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bioRxiv - Neuroscience 2023Quote: ... + 5% HS + 5% donkey serum (DS, Merck - D9663) at RT for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... 4% sucrose (Merck) in PBS for 20 minutes in room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight at 4°C with mEM48 (1:500, Merck Millipore, Cat. # MAB5374) in 1% normal goat serum and 0.1% Triton-X-100 in TBS ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4 °C with primary antibodies (GSX2: rabbit 1:200, Merck-Millipore ABN162 ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies were applied overnight at 4°C (monoclonal anti–IdU 1:4000; SAB3701448, Merck). Sections were incubated in biotinylated secondary antibodies (Jackson ImmunoResearch Labs ...
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bioRxiv - Biochemistry 2024Quote: ... and blocked overnight at 4°C with 1% bovine serum albumin (BSA, Merck, Cat# 7906) in PBS (200 µl per well) ...
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bioRxiv - Cancer Biology 2023Quote: ... DLD-1 cells expressing different EPHB1 wildtype or mutant versions and EFNB1 were mixed in suspension at a ratio of 1:3 and plated at a density of 130,000 cells/cm2 on coverslips coated with 2 mg/cm2 of 1-2 mg/mL laminin (Merck KGaA, Germany, USA) and incubated at 37ºC in 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... in PBS 5 min RT and stained with 1 μg/ml DAPI (Merck, D9542) in PBS for 10 min before mounting on slides with ProLong Gold Mountant (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... blots were blocked for 1 h and incubated overnight at 4 °C with anti-Amyloid fibrils (OC, 1:5000, Merck-Millipore), anti-prefibrillar oligomers (A11 ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged for 5 min at 20 000 x g and supernatants were immunoprecipitated for 1 h rotating at 4°C with mouse anti-GFP antibody (1 µg antibody per sample, 11814460001 Merck/Sigma) and 20 µl DynabeadsTM Protein G (10004D ...
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bioRxiv - Synthetic Biology 2024Quote: ... IL) for 10 minutes, followed by base piranha cleaning (1:1:4 H2O2 (000855032300, Bio-Lab, IL):NH3 (105432, Merck, DE):H2O ...
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bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal anti-Centrin-2 (1:250, clone 20H5, 04-1624, Merck Millipore), rabbit polyclonal anti-WDR90 (1:250 ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-Vinculin (Clone H Vin 1 0, 2 Ml; Cat. #V9131) from Merck. Anti-HA-11 epitope tag ...
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bioRxiv - Cell Biology 2023Quote: ... Nonspecific antibody blocking was for 1 h with 2% bovine serum albumin (Merck) in Tris-buffered saline ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 2 U mL−1 catalase from bovine liver (Merck-MilliPoreSigma, ref. C1345). NADH was added at a final concentration of 1 mM ...
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bioRxiv - Neuroscience 2023Quote: ... for 2h before clearing in benzyl alcohol:benzyl benzoate (1:2) (Sigma-Aldrich-Merck). Samples were stored in this solution at room temperature and protected from light.
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bioRxiv - Microbiology 2022Quote: ... the samples were reduced by 1 mM tris(2-carboxyethyl)phosphine (TCEP, Merck) and free sulfhydryl groups carbamidomethylated using 5.5 mM chloroacetamide (Sigma-Aldrich) ...
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Caloric restriction prevents inflammation and insulin dysfunction in middle-aged ovariectomized micebioRxiv - Physiology 2023Quote: ... 400 µL of 1% 2-thiobarbituric acid (TBA; Merck, St. Charles, MO, USA), and 200 µL of 20% phosphoric acid and heated at 100 °C for 15 min ...
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bioRxiv - Cancer Biology 2024Quote: ... H & L Chain Specific Peroxidase Conjugate (Merck, Cat#: 401315-2 ML, 1:5000). Proteins were detected by chemiluminscent detection system (Tanon ...
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bioRxiv - Cancer Biology 2024Quote: ... H&L Chain Specific Peroxidase Conjugate (Merck, Cat#: 401215-2 ML, 1:5000), or Goat Anti-Rabbit IgG ...
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bioRxiv - Synthetic Biology 2024Quote: ... followed by day 2 inoculation into 1 mL overnight autoinduction media (Merck, USA) with 50 µg/mL kanamycin ...
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bioRxiv - Bioengineering 2021Quote: Three sender area squares (3 × 3 mm) were put into a 24-well containing 300 μL of anti-His antibody (Merck, Germany, NOVG70796-3) (10 μg mL−1 in DPBS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: HPLC separations were performed on a Waters 2695 separations module equipped with a Waters 2996 photodiode array detector and HPLC analysis was carried out using a LiChrospher® 100 RP-18 column (4 mm × 250 mm, 5 μm) (Merck, Kenilworth, NJ, USA). The mobile phase consisted of two solvent reservoirs ...
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bioRxiv - Plant Biology 2020Quote: Inflorescences were harvested into fresh fixative (3:1 96% [v/v] ethanol [Merck] and glacial acetic acid) and kept overnight (O/N ...
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bioRxiv - Biochemistry 2022Quote: ... and concentrated to 13.7 mg·ml-1 (A280=23.36) using a centrifugal filter (Amicon Ultra-0.5, MWCO 3 kDa, Merck Millipore) prior to crystallization ...
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bioRxiv - Biochemistry 2022Quote: ... 1 % (w/v) Bovine serum albumin and 3 % (v/v) goat serum (Merck Life Science cat. G9023). Following blocking ...
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bioRxiv - Genomics 2024Quote: ... Traps deployed by BCC were also baited with 1-octen-3-ol (Merck Life Science, Bayswater, Australia) (van Essen et al. ...
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bioRxiv - Immunology 2022Quote: ... The coverslips were treated with 1% v/v solution of (3-acryloxypropyl)trimethoxysilane (APS, Merck - Sigma Aldrich) in ethanol for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... cells were fixed and permeabilized with a 3:1 mixture of methanol (Klinipath)-glacial acetic acid (Merck) for 10 min ...
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bioRxiv - Bioengineering 2022Quote: ... The coverslips were treated with 1% v/v solution of (3-acryloxypropyl)trimethoxysilane (APTS, Merck - Sigma Aldrich) in ethanol for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... incubated with rabbit-α-pH3Ser10 (1:250 for 3 hours at room temperature; Merck Millipore, Burlington, MA), incubated with goat-α-rabbit-AF568 (1:250 for 45 minutes at room temperature ...