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Citations for Merck :
601 - 650 of 1844 citations for Mouse Anti Cytomegalovirus Glycoprotein H gH 0861 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Viability of SH-SY5Y cells after treatments with or without H-LIPEF was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA) assay ...
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bioRxiv - Molecular Biology 2020Quote: ... Three individual clones were picked and amplified in LB broth including antibiotics for 12 h at 37°C and isolated using GenElute– HP Plasmid MaxiPrep Kit (Merck). Integrity of purified expression constructs was validated by gel electrophoresis and the concentration was determined by absorbance measurement ...
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bioRxiv - Bioengineering 2021Quote: Isolation of fresh human PBMCs was initiated within 1 h after blood collection using Histopaque® 1077 (10771; Merck KGaA) and standard density centrifugation (800 rcf ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 h at room temperature (RT) and revealed by chemiluminescence using Immobilon Crescendo or Forte Western HRP substrate (Millipore Merck).
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bioRxiv - Bioengineering 2022Quote: ... Viral supernatant was harvested at 48 and 72 h post-transfection and filtered through a 0.45 μm PVDF membrane (Merck Millipore). For lentiviral infection ...
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bioRxiv - Developmental Biology 2020Quote: ... for 1 h at 50 V followed by 3.5 h at 100 V and blotted on PVDF-membranes (Immobilon-PSQ Membrane, Merck Millipore). Incorporation of [35S]-methionine into newly synthesized proteins enabled the detection of translation products by phosphor imaging (exposure time of 1 day).
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bioRxiv - Neuroscience 2020Quote: Fifteen μl of sample were run in 12.5% SDS-PAGE gels at 100V for 2 h and then blotted to 0.45 μm low-fluorescence PVDF membrane (Millipore-Merck, USA) at 100V for 50 min ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed and stained with indicated primary antibodies for 2 h at RT followed by incubation with PLA probes (Merck, Duolink® In Situ PLA® Probe Anti-Rabbit PLUS ...
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bioRxiv - Bioengineering 2022Quote: ... gels were washed for around 2-4 h with PBS and incubated in 1 ml 1 μM Alexa Fluor 546 NHS ester (NHS-AF546, #A20002, Merck), overnight at room temperature on a slowly rotating shaker ...
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bioRxiv - Immunology 2022Quote: ... the sections were treated with 10% methanol in PB for 20 min and then incubated for 1 h at RT in incubation buffer: 5% normal donkey serum (NDS: Merck) and 0.2% Triton X-100 (Merck ...
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bioRxiv - Microbiology 2022Quote: ... Discs were aseptically removed after 48 h and dipped briefly in sterile PBS (Sigma-Aldrich, Merck Lifesciences Ltd, Dorset, UK), to remove any planktonic bacteria after which they were placed into 3 mL of one of the test agents ...
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bioRxiv - Cell Biology 2023Quote: ... Viral supernatants were harvested after at least 24 h of incubation at 32°C and filtered on a 0.45 µm filter (Merck #SE2M230I04).
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bioRxiv - Biochemistry 2023Quote: ... membrane using wet transfer for 3 h at 90 V/4 °C in transfer buffer (25 mM Tris; 192 mM glycine, Merck; 20% methanol, Merck). Afterwards ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 h at 4°C then moved to 37°C and incubated for 1 h with or without 100 µM cytochalasin D (Merck), 100 µM jasplakinolide (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatants of lysed cells were incubated in shake tubes over 16 h at 4°C with c-Myc antibodies (9E10 M5546, Merck). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was supplemented with calcium chloride to a final concentration of 3 mM and chromatin was solubilised for 2 h at 4 °C by digestion with Turbonuclease (T4330, Merck) to a final concentration of 250U/ml ...
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bioRxiv - Cell Biology 2023Quote: ... Media containing high titre pseudo virus was collected at 4 h intervals after the first media exchange and continuously for 48 h thereafter and filtered through a 0.45 μm PES filter (Merck, SLHP033RS) to remove cell debris ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant of the transfected cells was collected 48 h later and filtered through a 0.45 μm pore-size filter (Merck Millipore). For viral infection ...
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bioRxiv - Neuroscience 2023Quote: ... brain sections were washed three times for 15 min with 1X TBS and then incubated for 1.5 h in blocking solution: 2.5% Donkey Serum (Sigma, Merck, Germany, S30); 2.5% Goat Serum (Sigma ...
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bioRxiv - Pathology 2023Quote: Cryosections of formalin-fixed lung tissue 7 μm thick were washed in PBS and permeabilized for 1 h with 0.01% Tween 20 (Sigma-Merck, Germany), followed by three washes in PBS ...
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bioRxiv - Plant Biology 2024Quote: ... digestion of roots was performed at 90°C during 4 h in 400 µL of 65%(w/v) HNO3 (Suprapur, Merck). Mineralized samples were diluted in 0.65% (w/v ...
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bioRxiv - Immunology 2024Quote: ... For intracellular cytokine labelling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (50ng/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Immunology 2023Quote: ... For intracellular cytokine labelling cells were incubated for 3 h at 37°C in RPMI-1640+10% FBS with PMA (50ng/ml, Merck), Ionomycin (1μg/ml ...
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bioRxiv - Biophysics 2024Quote: ... Germany) for 4 h at 4°C followed by a washing in 0.1 M Soerensen’s Phosphate Buffer (Merck, Darmstadt, Germany). The dehydrating step was performed in the ascending alcohol series with 10 minutes duration each ...
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bioRxiv - Developmental Biology 2021Quote: ... The tip epithelial cells were infected with the modified lentiviral vector for 24 h at 37°C in a single cell suspension in the SN medium containing 10 μM Y-27632 (Merck, 688000). After 24 h ...
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bioRxiv - Neuroscience 2020Quote: ... cryoprotected for 48 h in 20% sucrose at 4°C and then snap frozen in isopentane (2-methylbutane, Merck GmbH, Austria) for 3 min at −60°C ...
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bioRxiv - Cell Biology 2021Quote: ... EVs containing fractions from 24 h conditioned medium were concentrated using a 100 kDA filter (Amicon Ultra-15, Millipore, Merck, Irealnd) and total RNA was isolated as previously described ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... neurons were stained with 1 μg/ml HOECHST-33342 (H-33342) and 1 μM calcein-AM (both from Merck, Darmstadt, Germany) one hour prior to the imaging ...
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bioRxiv - Molecular Biology 2019Quote: ... All patients underwent ovarian puncture (OPU) of follicles ≥15 mm in size after 36 h of hCG administration (Ovitrelle, Merck Serono).
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bioRxiv - Neuroscience 2021Quote: ... the kinetics of ROS production was evaluated for 2 h after the addition of 2,7-dichloro-diidrofluorescineacetate (DCFH-DA, Merck, Darmstadt, Germany) using the Microplate Reader GloMax fluorimeter (Promega Corporation ...
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bioRxiv - Zoology 2023Quote: ... it was fractionated into the different fatty acid classes over an activated silicic acid column (heated at 120ºC for 2 h; Merck Kieselgel 60) via eluting with 7 ml chloroform ...
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bioRxiv - Synthetic Biology 2022Quote: ... This solution was incubated at 37°C for 3-4 h before being transferred to a 250 mL flask containing 20 mL of 425-600 μm glass beads (Merck G9268) and 30 mL of sterile MilliQ water ...
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bioRxiv - Microbiology 2023Quote: ... bacteria were freshly grown for 20 to 24 h on plates in anaerobic jars supplemented with Anaerocult C sachets (Merck, USA) at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... and incubated at 28°C shaking at 200 rpm for 2–3 h before plating on LB agar supplemented with 50 µg/ml Kanamycin (Merck Millipore). The plates were incubated for 2– 3 days at 28°C ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were pre-cleared at 4°C for 1 h on a rotating wheel using 30 µl Protein-S agarose beads (Merck Millipore) slurry ...
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bioRxiv - Plant Biology 2023Quote: ... then 200 µl of each sample was digested for 16 h at 37 °C with α-amyloglucosidase (6 U/ml, Merck), and α-amylase (4 U/ml ...
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Endothelial transmigration hotspots limit vascular leakage through heterogeneous expression of ICAM1bioRxiv - Immunology 2022Quote: ... VCAM-1 monoclonal mouse antibody was purchased from Merck (MAB2511). Alexa Fluor 647-conjugated PECAM-1 monoclonal mouse antibody was bought from BD (561654 ...
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bioRxiv - Bioengineering 2020Quote: ... and 0.6μg/ml (2x) Laminin (Mouse purified, Merck Millipore CC095). The agarose solution was mixed 1:1 with the cells/fibrinogen/laminin in 2i solution and kept at 37°C briefly to avoid polymerisation ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse antibody against detyrosinated tubulin (1:100; MERCK, AB3201) each of which was diluted in blocking solution ...
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bioRxiv - Cell Biology 2020Quote: ... TE-7 (mouse, 1:200, Cat nb CBBL271, Merck, Sigma), Pax7 (mouse ...
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bioRxiv - Plant Biology 2021Quote: ... we applied the immunolabeling assay using primary mouse (Sigma Merck) or rabbit (Sigma Merck ...
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bioRxiv - Molecular Biology 2019Quote: ... Mouse oocytes post injection were cultured in M16 media (Merck) supplemented with IBMX in 5% CO2 at 37 °C for 20 hours ...
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bioRxiv - Immunology 2020Quote: ... Primary mouse keratinocytes were cultured in custom-made (Biochrom, Merck) DMEM/HAM’s F12 (FAD ...
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bioRxiv - Neuroscience 2022Quote: ... and mouse monoclonal α-TH (1:2000; Merck Millipore MAB318) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: Mouse primary hepatocytes were isolated by collagenase (C2-28, Merck) digestion of livers from ad libitum fed mice using two-step collagenase perfusion technique as described previously40 ...
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bioRxiv - Bioengineering 2023Quote: ... and mouse monoclonal Flag antibody (clone M2, 1:1000, Merck). The following secondary antibodies were used ...
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bioRxiv - Developmental Biology 2023Quote: ... 1,000 U/ml mouse leukemia inhibitory factor (mLIF) (Millipore, Merck), 1 μM PD0325901 (Peprotech ...
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bioRxiv - Microbiology 2023Quote: ... Cells were stained with mouse monoclonal 4G2 antibody (Merck Millipore) and AlexaFluor488 anti-mouse secondary antibody (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... and Olig2 (monoclonal mouse, 1:250, MABN50, Merck Millipore, USA). Secondary antibody conjugated with Alexa-488 was used (1:250 or 1:500 ...
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bioRxiv - Pathology 2024Quote: The primary mouse cardiomyocytes were cultured on laminin (Merck, L2020) surface-coated four well Permanox slides (Thermo Fisher Sci ...