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Citations for Merck :
601 - 650 of 936 citations for 4 4 Bromophenylsulfonyl morpholine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... supplemented with 2 % (vol/ vol) FCS and and 10 mM 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES, Merck) and cut into 1 cm pieces ...
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bioRxiv - Physiology 2022Quote: ... containing 150 μL 4°C DMEM (Dulbecco’s modified Eagle’s medium with high glucose and 25mM HEPES from Sigma Aldrich (Merck), UK ...
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bioRxiv - Physiology 2020Quote: ... Membranes were probed overnight at 4°C with anti-phosphotyrosine antibody (1/1000, 05-321, clone 4G10, Merck Millipore) in blocking solution ...
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bioRxiv - Synthetic Biology 2021Quote: ... VLP fractions were pooled and concentrated using 100 kDa MW cut-off centrifugal filters (Amicon Ultra 4 ml, Merck). Protein concentrations were measured using the linearised Bradford method54 with Pierce Coomassie Protein Assay Kit (Thermo Scientific).
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bioRxiv - Genomics 2022Quote: ... Total chromatin was pre-cleared for 1h at 4°C with rotation using protein A sepharose beads (P9424, Merck). 3 µg of anti-histone H3 (Abcam ...
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bioRxiv - Immunology 2020Quote: ... with 1μg/mL anti-CD3 and 1μg/mL anti-CD28 at 4°C at a density of 105 cells/m in starvation media (RPMI (Merck), containing 0.5% Heat Inactivated FCS (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The virus-containing lysate was purified via iodixanol density-gradient ultracentrifugation (290,000 × g, 120 min, at 4°C) and rebuffered in PBS using amicon spin columns (Merck). Particles in PBS were stored in aliquots at −80°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were collected at 20,000 x g for 40 min at 4°C and soluble proteins in the supernatant were sterile filtered 5µm PVDF Membrane (Merck).
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bioRxiv - Immunology 2021Quote: ... Eluted RBDQ498Y and ACE2 were then concentrated and buffer-exchanged to TBS pH 7.4 using 10 kDa and 50 kDa 4 mL Amicons (Merck), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... 4561086) and transferred at 4°C to either Immobilon-FL or Immunoblon-P (IPVH00010) polyvinylidene fluoride membranes (Merck, IPFL00010) (1.5 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were separated on a 4–15% gel by SDS-PAGE and transferred to PVDF-FL membranes (IPFL00010, Merck). Membranes were blocked with 5% non-fat milk in PBS and incubated with primary antibodies diluted in 5% non-fat milk in PBS+0.1%Tween-20 (PBS-T ...
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bioRxiv - Microbiology 2023Quote: ... 108 – 109 PFU (plaque-forming units) mL-1) in TSB (tryptone soya broth, Oxoid) or quarter-strength (1/4) Ringer’s buffer (Merck)) was used in this study ...
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bioRxiv - Cancer Biology 2023Quote: ... was added for 16-18 hr at 4°C before washes and the addition of anti-phospho H2A.X mAb (Merck, Kenilworth ...
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bioRxiv - Biochemistry 2023Quote: ... an aliquot was thawed and diluted to 4 μM in 1xPBS supplemented with 0.01% NaN3 (Merck, Cat. No. 71290) and incubated at 37 °C under quiescent conditions for one week ...
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bioRxiv - Biophysics 2023Quote: ... Peak fractions were pooled and concentrated in an Amicon Ultra-4 Ultracell 30 kDa centrifugal filter (Merck-Millipore #UFC803024). Aliquots were snap frozen and stored at −80 °C ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 24 h at +4°C with primary antibodies (goat anti-Chat (1:200, Merck, AB144P), mouse anti-GFAP (1:300 ...
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bioRxiv - Microbiology 2022Quote: ... To each tube 1 μL of Tris((1-benzyl-4-triazolyl)methyl)amine (TBTA) solution (2.5 mM in DMSO; Merck), 10 μL of Tetrakis(acetonitrile)copper(I ...
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bioRxiv - Molecular Biology 2024Quote: The cell pellet was fixed in a mixture of 4% formaldehyde and 0.1% glutaraldehyde in 0.1M HEPES (Sigma-Aldrich / Merck) for 1 h at RT ...
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bioRxiv - Microbiology 2024Quote: ... samples maintained at 4°C were eluted through a ZIC-pHILIC column (5 μm, polymeric, 150 by 4.6 mm; SeQuant, Merck) by mobile phase A (20 mM ammonium carbonate ...
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bioRxiv - Genomics 2024Quote: ... media was changed for 500 µL of DMEM +5% FBS +4 μg/mL of polybrene (Merck TR-1003-G). Lentiviruses were diluted to the desired MOI in 500 µL of DMEM +5% FBS and slowly added to each well ...
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bioRxiv - Microbiology 2023Quote: ... 100 ml of the cell suspension at an OD660 of 0.6 was harvested by centrifugation at 5,000×g for 10 min at 4°C and resuspended in 2 ml of BugBuster (Merck), and 50 μg/ml lysozyme and 1 mM phenylmethanesulfonyl fluoride (PMSF ...
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bioRxiv - Developmental Biology 2023Quote: Axolotl embryos of genotype tgSceI(Mmu.Prrx1:TFPnls-T2A-ERT2-Cre-ERT2; Caggs:loxP-GFP -loxP-mCherry)Etnka were treated with 4-OHT (Merck H7904) as described in 48 to permanently label connective tissue cells with mCherry ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysate was then spun at 20,000 g for 20 min at 4°C and loaded to a HisTrap HP column (Merck). The protein was eluted with the buffer with 300 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... was performed on 4 and 2.5 mg protein lysates for timsTOF optimization and dual-probe analyses respectively in 2 M urea (Merck) in 1× 50 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2023Quote: ... Calibration curves were acquired using defined amounts of the fluorescent product 4-methylumbelliferone sodium salt (#M1508, Sigma-Aldrich/Merck). Fluorescence of 4-methylumbelliferone was normalized to crystal violet intensity.
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bioRxiv - Biochemistry 2023Quote: ... The protein was concentrated by ultrafiltration (4°C, 3,500 x g) using Amicon Ultra Centrifugal Filter Units 10K molecular weight cut-off (MWCO) (Merck). CT produced in E ...
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bioRxiv - Biochemistry 2022Quote: ... aliquots of 4 mL of the culture were vacuum-filtrated using 0.45 μm pore size filters (HVLP02500, Merck Millipore). The filters were transferred to -20°C cold 40:40:20 acetonitrile:methanol:water for metabolite extraction ...
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bioRxiv - Immunology 2022Quote: ... The sections were incubated overnight at 4°C with the following primary antibodies: KRT8 (Merck Millipore, MABT329, 1:100), p-ERK (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were stained with DAPI before mounting with Mowiol (12 mL of 0.2 M Tris buffer pH8.5, 6 mL distilled water, 6 g glycerol, 2.4 g Mowiol 4-88, Merck Millipore).
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bioRxiv - Neuroscience 2023Quote: ... Coverslips were shortly rinsed in distilled water and mounted using Mowiöl (12 ml of 0.2 M Tris buffer, 6 ml distilled water, 6 g glycerol, 2.4 g Mowiol 4-88, Merck Millipore). Samples were imaged directly or within the next 48h ...
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bioRxiv - Neuroscience 2023Quote: ... 1c and Fig.3 were first fixed with 4% PFA solution for 30 minutes at RT and quenched with 0.1 M glycine (Merck) solution in PBS for 15 minutes at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were fixed on 4% PFA and senescence determination was made by measuring SA-β-gal activity (KAA02 Merck) and IF against the markers of senescence ...
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bioRxiv - Bioengineering 2023Quote: ... for 20 min at room temperature (RT) and permeabilised overnight at 4 °C in 0.5% Triton X-100 (Merck) solution in PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions between 15.7 mL to 19.2 mL in elution volume were concentrated with Amicon Ultra-4 Centrifugal Filter Unit (10 kDa cutoff, Merck) to ∼2 mg/mL (measured by Bradford method) ...
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bioRxiv - Biochemistry 2023Quote: ... concentration and buffer exchange to TE (10 mM Tris-HCl pH7.5 and 1 mM EDTA) with an Amicon Ultra-4 centrifuge filter unit42 (30 kDa cut-off, Merck). Concentration of all plasmids was measured by NanoDrop One (Thermo Scientific ...
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bioRxiv - Physiology 2024Quote: ... Membrane was incubated overnight at 4°C with specific mouse monoclonal primary antibody anti-puromycin (Merck, MABE343, 1:1000) and after wash ...
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bioRxiv - Genetics 2024Quote: ... The harvested medium was then centrifuged at 1,300 rpm at 4 °C for 5 min to remove cells and then filtered by 0.45 μm filter (Merck) and the virus was collected and stored at -80 °C.
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bioRxiv - Microbiology 2024Quote: ... The pellet containing the FoIV1-rORF4 inclusion bodies was obtained by centrifugation at 12,000 × g for 20 min at 4°C and then suspended in 0.1× BugBuster Protein Extraction Reagent (Merck KGaA) using a homogenizer ...
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bioRxiv - Biochemistry 2024Quote: ... Fixed cells were stained with DAPI and mounted on Mowiöl (12 ml of 0.2 M Tris buffer, 6 ml distilled water, 6 g glycerol, 2.4 g Mowiol 4-88, Merck Millipore) for imaging.
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bioRxiv - Biochemistry 2024Quote: ... containing kinase assay buffer (final concentration: 40 mM HEPES, 120 mM NaCl, [pH 7.5] and 0.3% CHAPS, 4 mM MnCl2 (Merck, 1059270100), 10 mM DTT (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... 2.7 mL of each sample was transferred into an Amicon Ultra-4 3K (3000 MWCO) filtration tube (Merck Millipore) and centrifuged for 40 minutes at 6225 g ...
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bioRxiv - Plant Biology 2024Quote: ... The lumen proteins were concentrated at 14,000 rpm for 10 min at 4 °C using Amicon ultra-0.5 centrifugal filters (Cat. No. UFC500324, Merck millipore). All solutions contained protease inhibitors with final concentration of 1 mM benzamidine ...
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bioRxiv - Biochemistry 2024Quote: ... for 4 h at 4°C and then concentrated to 200 ng/μl using Amicon Ultra-0.5 centrifugal filter units (Merck). Nucleosomes are then diluted with ice-cold 0 M FRET buffer to a concentration of 50 nM ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by overnight incubation at 4°C with 250µl of the anti-DIG antibody (Merck #11093274910 diluted 1:2000) and other necessary primary antibodies (when additional immunostaining was needed ...
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bioRxiv - Developmental Biology 2024Quote: ... EBs were permeabilised with 0.5% Triton X-100/PBS for 1 hour and blocked for 1 hour with 4% donkey serum/PBS (Merck), both at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... through six consecutive dilution and concentration steps at 4 °C using Amicon Ultra centrifugal filters with a 3 kDa or 10 kDa molecular weight cutoff (Merck). Protein complexes were assembled by mixing the subcomponents at the desired molar ratios ...
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bioRxiv - Biophysics 2021Quote: ... 250 ng of each plasmid encoding VN and VC were transfected using 4 μL of 1 μg/mL PEI (Merck) for each μg of DNA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were cryopreserved at a concentration of 4*106 cells/ml in 90% N2-S medium and 10% dimethyl sulfoxide (DMSO) (Merck Millipore ...
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bioRxiv - Biophysics 2021Quote: ... fluorescently-labeled and biotinylated H57-scFV were concentrated to 0.2 - 1 mg/mL with 10 kDa Amicon®Ultra-4 centrifugal filters (Merck) and stored in 1x PBS supplemented with 50 % glycerol at -20°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... at 4°C in dialysis buffer (100 mM NaCl, 20mM Tris pH 7.4, containing 15 μl bovine thrombin (605157, Merck Millipore) to cleave the His-tags) ...