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Citations for Merck :
601 - 650 of 1791 citations for 2 p sec Butylphenyl propionic Acid d3 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and were transferred at 100 mA for 70 min onto PVDF membrane (Immobilon-P, 0.45 μM pore size, Merck Millipore) using semi-dry blotting system ...
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bioRxiv - Neuroscience 2021Quote: ... samples were heated 5 minutes at 95°C and 30µl were separated by SDS-PAGE and transferred onto PVDF membranes (Immobilion-P, Merck Millipore) according to standard protocols ...
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bioRxiv - Cell Biology 2021Quote: The samples were loaded onto a 5–20% gradient SDS-PAGE gel (Wako, Osaka, Japan) and transferred to an Immobilon-P Transfer Membrane (Merck). Antibodies were diluted with Signal Enhancer HIKARI for Western Blotting and ELISA (Nacalai Tesque) ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were blocked and incubated with primary antibodies against bornavirus P (1:500) and α-tubulin (Merck, Darmstadt, Germany), followed by incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2021Quote: ... Denaturated and fully-reduced proteins were resolved on Tris-glycine SDS-PAGE followed by western blot analysis onto PVDF membrane (Immobilon-P, 0.45 μM pore size, Merck Millipore) using enhanced chemiluminescence to detect bound antibodies (Pierce) ...
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bioRxiv - Microbiology 2021Quote: ... lysed in Laemmli SDS-loading dye and separated in 15% acrylamide gels and transferred to 0.45 μm hydrophobic Immobilon-P PVDF membrane (Merck Millipore). Immunoblotting was as described previously 19 ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were loaded and resolved using a 10% SDS-PAGE gel and the separated proteins were transferred onto a PVDF membrane (IPVH00010, Immobilon-P; Merck). Blocking was performed using 5% Skimmed milk (70166 ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μg proteins were separated by SDS-PAGE and electrotransferred onto Immobilon®-P transfer membranes (Merck Millipore, MA, USA), blocked with 5% skim milk in Tris-buffered saline with 5% Tween 20 (TBST) ...
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bioRxiv - Cell Biology 2020Quote: ... in ice-cold serum-free RPMI media (R5886, Merck, with 100 U/μg/ml penicillin/streptomycin [P/S, P0781, Merck] and 2 mM L-glutamine [L-glut ...
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bioRxiv - Immunology 2020Quote: ... The proteins were then separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred onto an Immobilon-P membrane (IPVH00010; Merck). The membranes were blocked with 10% nonfat milk (190-12865 ...
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bioRxiv - Neuroscience 2024Quote: ... and equal amount of protein were separated by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Immobilon-P, Merck Millipore). Membranes were blocked for one hour in I-BlockTM (# T2015 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated on a 10% polyacrylamide gel and transferred to polyvinylidene difluoride membrane (Amershan Hybond P PVDF membrane, Merck). Membranes were blocked in 5% milk in PBS/0.1% Tween-20 ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were concentrated in a buffer containing 20 mM HEPES-KOH (pH 7.5) and 0.2% Noidet P-40 using a dialysis spin column (Amicon Ultra-10; Merck, Darmstadt, Germany). The concentrations of purified fluorescent proteins were measured using fluorescence correlation spectroscopy (FCS) ...
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bioRxiv - Physiology 2024Quote: ... the pancreas was perfused via the pancreatic duct using an ice-cold collagenase solution (Collagenase P, 11213865001, Merck/Sigma Aldrich) with a concentration of 0.63 mg/ml ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then washed with three rounds of PBS before incubation in 200 µl p-Nitrophenol phosphate substrate solution for ELISA (Merck) for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... Wells were again washed three times with PBS and bound alkaline phosphatase quantified using a p-Nitrophenol phosphate substrate solution for ELISA (Merck) and A405 readings.
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bioRxiv - Biochemistry 2024Quote: ... For the determination of the Ki inhibitory constant of macrocyclic peptide inhibitor MP5.4.3 against PT5 we used chromogenic substrate N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide (100 μM; Merck, Darmstadt, Germany) at final concentration of 100 μM ...
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bioRxiv - Cell Biology 2020Quote: ... washed in PBS and incubated for 2 min in 2 mM H2O2 (Merck) at room temperature ...
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bioRxiv - Immunology 2023Quote: ... or 1% FCS (Biowest) and 2 mM CaCl2 and 2 mM MgCl2 (Merck).
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bioRxiv - Cell Biology 2023Quote: ... or 2-deoxy-glucose (2-DG; 500 μg/g) (25972, Merck Life Sciences) were injected IP at Day 0 ...
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bioRxiv - Microbiology 2022Quote: ... solvent B = acetonitrile with 0.1% formic acid (purity: MS-grade; Merck KGaA, Darmstadt, Germany), t (time)=0 min ...
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bioRxiv - Biophysics 2021Quote: ... Boric acid (99.5%-100.5%) and sodium hydroxide (≥ 99.0 %) was acquired from Merck (Darmstadt, Germany). Acetic acid was obtained from VWR Chemicals (Leuven ...
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bioRxiv - Systems Biology 2020Quote: ... At first cells were resuspended in trifluoroacetic acid (TFA) (Uvasol® for spectroscopy, Merck) (sample/TFA 1:4 (v/v) ...
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bioRxiv - Neuroscience 2020Quote: ... the cerebellar slices were treated directly with glutamate (L-Glutamic acid, Sigma-Aldrich, Merck) twice every 24hours at a concentration of 75μM and 100μM ...
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bioRxiv - Biochemistry 2021Quote: ... with a continuous flow of 0.1% formic acid in water (UPLC grade, Merck, GE) at 100 μl/min ...
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bioRxiv - Neuroscience 2021Quote: ... 100 µM L-ascorbic acid and penicillin/streptomycin (all from Merck, Missouri, United States). The culture medium was changed every 2–3 days ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were extracted in 70% ethanol plus 5% formic acid (Merck-Millipore) twice for 20 min with permanent shaking ...
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bioRxiv - Immunology 2022Quote: ... HLA-peptide complexes were eluted by repeated addition of 0.2% TFA (trifluoroacetic acid, Merck). Eluted HLA ligands were purified by ultrafiltration using centrifugal filter units (Millipore) ...
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bioRxiv - Neuroscience 2020Quote: ... at 65°C and in MAB solution (maleic acid buffer; Sigma-Aldrich/Merck, Germany) at room temperature ...
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bioRxiv - Plant Biology 2021Quote: 2,5-Dihydroxybenzoic acid (2,5-DHB) and ultrapure water were purchased from Merck (Darmstadt, Germany). Indium-thin-oxide (ITO ...
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bioRxiv - Molecular Biology 2020Quote: ... Afterwards 300 μl of acid washed glass beads (425-600 μM from Sigma/Merck) were added to the cell suspension and kept in ice for 5 min then tubes were vortexed three times with 1 min incubation on ice between each round ...
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bioRxiv - Biophysics 2021Quote: Fmoc (9-fluorenylmethyloxycarbonyl)-amino acids were obtained from Novabiochem (Merck Biosciences, La Jolla, CA). Rink amide MBHA resin (0.65 mmol/g ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Tryptic peptides were acidified to a final concentration of 1% formic acid (FA) (Merck), cleaned up using SepPak cartridges (Waters) ...
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bioRxiv - Biochemistry 2022Quote: ... a control solution with identical pH was prepared with hydrochloric acid (1.09057, Merck KGaA). In addition to the pH ...
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bioRxiv - Bioengineering 2022Quote: ... and MEM non-essential amino acid solution (100x) were purchased from Merck (Darmstadt, Germany). L-glutamine (200 mM) ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were acidified to a final concentration of 1% Trifluoroacetic acid (Uvasol; MERCK KgaA) prior to immobilizing the beads on the magnetic rack ...
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bioRxiv - Biochemistry 2024Quote: ... formic acid (FA) and SeQuant ZIC-HILIC column were purchased from Merck (Darmstadt, Germany). Acetic acid (AA) ...
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bioRxiv - Developmental Biology 2023Quote: ... The IgG was eluted with 150 µL 0.1 M formic acid (Merck KGaA, Darmstadt) directly into a PCR plate containing 15 µL 1 M ammonium bicarbonate (Acros Organics ...
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bioRxiv - Biochemistry 2023Quote: ... Protein expression was performed in the presence of 3.5 mM valproic acid (Merck Millipore) and 10 μM kifunensine (Biomol) ...
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bioRxiv - Developmental Biology 2023Quote: ... We used Hoyer’s mounting medium in a1:1 proportion with lactic acid (90% MERCK) to preserve the cuticle of the legs ...
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bioRxiv - Microbiology 2023Quote: ... formic acid and methanol used for micropollutant analysis were HPLC-gradient grade (Merck, Germany).
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bioRxiv - Cancer Biology 2023Quote: ... Samples were acidified to a final concentration of 1% Trifluoroacetic acid (Uvasol; MERCK KgaA) prior to immobilizing the beads on the magnetic rack ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were acidified to a final concentration of 1% Trifluoroacetic acid (Uvasol; MERCK KgaA) prior to immobilising the beads on the magnetic rack ...
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bioRxiv - Microbiology 2024Quote: ... Nucleic acids were labeled with 3 µM DAPI (Ex/Em: 352/464, Merck, Belgium) for 20 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... Soluble proteins were quantified by bicinchoninic acid assay (BCA, Merck, Refs B29643 and C2284) and equal protein amounts (5 or 0.5 mg for IP followed by mass spectrometry or Western blotting ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were acidified to a final concentration of 1% Trifluoroacetic acid (Uvasol; MERCK KgaA) prior to immobilizing the beads on the magnetic rack ...
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bioRxiv - Neuroscience 2021Quote: ... The samples were separated by SDS–polyacrylamide gel electrophoresis and then transferred to a polyvinylidene difluoride (PVDF) membrane (Hybond-P PVDF membranes, Merck, Germany) by a wet-transfer apparatus (Vep-3 ...
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bioRxiv - Neuroscience 2020Quote: Samples boiled in 6x Laemmli buffer were subjected to standard SDS-PAGE (10%) prior to being electrophoretically blotted onto Immobilon-P polyvinylidene difluoride membranes (Merck, UK). Ponceau S staining (Merck ...
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bioRxiv - Neuroscience 2020Quote: Samples boiled in 6× Laemmli buffer were subjected to standard SDS-PAGE (10%) and electrophoretically blotted onto Immobilon-P polyvinylidene difluoride membranes (Merck, UK). Total protein was quantified using Ponceau S staining (Merck ...
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bioRxiv - Neuroscience 2021Quote: ... Equal amounts of 20 to 30 μg of protein were separated by SDS-PAGE and transferred onto PVDF membranes (Immobilion-P, Merck Millipore) according to standard protocols ...