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Citations for Merck :
551 - 600 of 742 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... 105 cells were seeded on the upper chamber of 24 well plate cell culture inserts containing 3 µm pores (Cat # 353096, Merck). The inserts were coated with rat-tail collagen I ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with a total of 1000 μg to 1500 μg DNA in 35 mm fluorodishes or 6 well plates using Genejuice as per the manufacturer’s instructions (Merck Millipore). Cells were typically imaged 48 h after transfection ...
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bioRxiv - Microbiology 2021Quote: ... PBMCs (2 × 106 cells) were plated onto 48-well plates (NalgeNunc) in RPMI-1640 with 5% inactivated male human AB serum (Merck) for 3 hours ...
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bioRxiv - Microbiology 2021Quote: ... Bacterial inoculums were subsequently prepared using the direct colony suspension method.40 Three to five bacterial colonies were obtained from the agar plate and inoculated into an Eppendorf tube containing 1 ml of cation-adjusted Muller-Hinton broth (caMHB, Merck), consisting of 20-25 mg/L calcium ions (Ca2+ ...
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bioRxiv - Microbiology 2020Quote: ... Real-time analyses were performed on solid medium in ibidi micro dishes and in liquid medium using a CellASIC ONIX platform and compatible B04A plates (Merck), as described previously (Hołówka et al. ...
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bioRxiv - Bioengineering 2020Quote: ... 50 μL of culture from underneath the pellicle was removed and placed into the inlet well of a B04A CellASIC ONIX plate (Merck). The plate was placed onto a fluorescence microscope (Nikon Eclipse Ti inverted microscope ...
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bioRxiv - Biochemistry 2020Quote: ... were incubated in standard assay buffer at 37°C and 2 µL aliquots were removed at appropriate time points and spotted on a silica gel 60 F454 plate (Merck). The separation was carried out in butanol:ethanol:milliQ water (5:3:2 ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 million cells were plated per well in a 6-well plate and cultured for 48h in the presence or absence of 200 nM smoothened agonist (Merck). RNA was extracted and cDNA was generated with Superscript II kit (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: Cells were sonicated at low power for 3 s and loaded onto a commercial microfluidics system (Y04C-02 plates, CellASIC ONIX2 system, Merck). While loading the cells ...
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bioRxiv - Plant Biology 2021Quote: ... PIP was separated from the rest of the phospholipids by thin-layer chromatography (TLC) using K-oxalate-impregnated and heat-activated silica gel 60 plates (20×20 cm; Merck) and an alkaline TLC solvent ...
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bioRxiv - Synthetic Biology 2021Quote: ... round bottomed deep well plate at 40 % liquid volume and sealed with a Breathe-easy sealing membrane (Merck, #2380059-1PAK). Overnight growth occurred in an Infors MT multitron pro shaker at 250 RPM rotating orbitally at 25 mm diametres at 37 °C for 17 h.
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bioRxiv - Plant Biology 2021Quote: ... surface sterilized with 10% (v/v) bleach solution and plated on agar plates containing one-half strength Murashige and Skoog (MS) salts (Merck) pH 5.7 ...
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bioRxiv - Plant Biology 2021Quote: ... Purity of compounds was monitored via thin layer chromatography (TLC) using pre-coated aluminium-backed plates (silica gel 60 F254, Merck) and compounds were visualised by UV radiation at 254 nm and then using an anisaldehyde spray reagent (1% p-anisaldehyde:2% H2SO4 ...
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bioRxiv - Microbiology 2021Quote: Reaction products and standards were dissolved in 20% 1-propanol and separated on a 10 cm HPTLC Si-60 plates (Merck) using 1-propanol:acetone:water 9:6:4 (v:v:v ...
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bioRxiv - Microbiology 2020Quote: ... The crude fractions were dissolved in EA (10 mg/ml) and 10 µl from each were spotted onto 20 × 20 cm TLC plates (Silica gel 60 F254, Merck) with at least one cm between the spots and placed in a binary mobile phase consisting of Chloroform ...
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bioRxiv - Microbiology 2020Quote: ... Twenty µl of the EA fraction (10 mg/ml) were spotted onto 20 × 20 cm TLC plates (Silica gel 60 F254, Merck) with at least one cm between the spots and placed in a quaternary mobile phase consisting of Benzene ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed in TBS and demi-water before being dried on a heat plate at 37°C before and cleared in xylene and mounted with Entellan (Merck).
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bioRxiv - Microbiology 2021Quote: ... Extracts were filled into a 100-μl Hamilton syringe and spotted onto silica gel 60 F254 high-performance thin-layer chromatography (HPTLC) plates (Merck) with a Linomat 5 sample application unit (Camag ...
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bioRxiv - Microbiology 2022Quote: ... and non-expressing (d2EGFP−) cells were seeded into a 96 well plate pre-coated with 1 mg/ml Poly-D-Lysine (PDL, Merck). Aryl hydrocarbon receptor (AhR ...
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bioRxiv - Microbiology 2020Quote: ... Materials from the organic and aqueous fractions were analyzed by one-dimensional TLC on a silica gel 60 plate (Merck) using a solvent system composed of chloroform:methanol:acetate:water (25:15:4:2 by volumes) ...
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bioRxiv - Genetics 2021Quote: ... At day 10 cells were passaged at a 2:3 ratio into 12 well cell culture plates coated with 15 µg/ml human plasma fibronectin (Merck) in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Microbiology 2020Quote: ... A total of 50 μg of protein from each sample was transferred to a protein LoBind plate (Merck, NJ, USA). Protein was reduced with tris (2-carboxyethyl ...
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bioRxiv - Biochemistry 2020Quote: The reaction was monitored by thin-layer chromatography (TLC) performed on precoated Merck 60 GF254 silica gel plates (Merck, US) with a fluorescent indicator ...
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bioRxiv - Bioengineering 2020Quote: ... and 100 μl dispensed in triplicate into wells of 96-well ELISA plates (Nunc Maxisorp; Merck Ltd., Poole, United Kingdom) preblocked with 1w/v BSA in PBS and precoated with monoclonal anti–IFN-γ (U-Cytech ...
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Biodiversity and Antimicrobial Potential of Bacterial Endophytes from Halophyte Salicornia brachiatabioRxiv - Microbiology 2020Quote: Purification of compounds was carried out by separating 50 mg of crude extract on preparative TLC plates (Kieselgel 60 F254, 25 mm, Merck) using the same solvent system as above ...
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bioRxiv - Molecular Biology 2020Quote: ... Lipid extracts corresponding to 1 mg wet liver weight were applied onto HPTLC silica gel 60 plates (10 × 20 cm: Merck) and plates were developed in n-hexane/diethylether/acetic acid 70:30:5 (v/v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were grown on gelatin coated plates which were prepared by first incubating the wells 0.1% gelatin (EmbryoMax® ES-006-B, Merck) with 1% FBS (EmbryoMax® ES Cell Qualified FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... cells in E8 medium were seeded into a V-shaped 96-well plate (6 000 cells/well) coated with Polyhema (Merck). After 48 hours (Day 0 of the differentiation process ...
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bioRxiv - Genomics 2021Quote: ... serial 10-fold dilutions were prepared from the viral stock and used to transduce mESCs in a 6-well plate (Mock plus 10-2 to 10-6) together with 8 ng/µl polybrene (Merck) in duplicates ...
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bioRxiv - Genetics 2021Quote: ... 5 serial 10-fold dilutions of the viral stock were applied to each well of a 6-well mESC plate (MOCK plus 10−2 to 10−6) for transduction with 8 ng/µl polybrene (Merck). Two replicates were generated for each well ...
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bioRxiv - Biochemistry 2021Quote: ... Lipids containing [6-3H]GlcN-PI were dissolved in 100 μL of chloroform: methanol (2:1, by volume) and loaded to a high performance thin-layer chromatography (HPTLC) plate (Merck). The [6-3H]GlcN-PI was located by phosphorimaging ...
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bioRxiv - Microbiology 2021Quote: ... NHS-ester staining: the gel was placed in a 6 well plate and incubated in 594 NHS-ester (Merck, 08741) diluted at 10 μg/mL in 3 ml PBS for 1 hour and 30 minutes at room temperature on a rocking platform ...
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bioRxiv - Molecular Biology 2021Quote: ... and the plates were incubated at 33 °C in a 5% CO2 atmosphere in fresh medium with 0.25% Hoechst (Merck-Millipore) to stain the DNA ...
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bioRxiv - Microbiology 2020Quote: ... for ELISpot detection in combination with multiscreen immunoprecipitation (IP) ELISpot polyvinylidene difluoride (PVDF) 96-well plates (Merck Millipore, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated onto the 96-well plates coated with poly-L-ornithine (Sigma-Aldrich, Merck KGaA, St. Louis, MO). Equal volumes of cell suspension were plated onto the center of the dish ...
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bioRxiv - Microbiology 2022Quote: ... appropriate amounts of polar lipid extracts were spotted onto silica gel high-performance thin-layer chromatography (HPTLC) plates (silica gel 60 F254, Merck) using a Linomat 5 sample application unit (CAMAG ...
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bioRxiv - Genetics 2022Quote: ... 4 serial 10-fold dilutions of the viral stock were applied to each well of a 6-well mESC plate (MOCK plus 10−3 to 10−6) for transduction with 8 ng/μl polybrene (Merck). Two replicates were generated for each well ...
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bioRxiv - Molecular Biology 2022Quote: ... Reaction progress was monitored with analytical thin-layer chromatography (TLC) on aluminum backed precoated silica gel 60 F254 plates (E. Merck). Column chromatography was carried out using silica gel 60 (230-400 mesh ...
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bioRxiv - Immunology 2022Quote: ... The mix was transferred to a plate coated with avidin and the FLAG-peptide was detected with an anti-FLAG-alkaline phosphatase conjugate (Merck), developed with pNPP SigmaFAST (Merck ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from 2-week-old seedlings grown on plates under 16-hour photoperiod using RNAzol reagent (Sigma-Aldricht, Merck) according to the manufacturer’s instructions and DNA precipitation with a 4-fold volume of bromoanisole ...
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bioRxiv - Plant Biology 2022Quote: ... Five μl of each reaction mixture were loaded onto a TLC plate (TLC Silica gel 60 F254, Merck, Darmstadt, DE). The mobile phase was 7 vol-parts of 1-propanol mixed with 8 vol-parts of H2O ...
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bioRxiv - Plant Biology 2022Quote: ... The purity of compounds was checked using 1H NMR or thin layer chromatography (TLC) using pre-coated aluminium-backed plates (silica gel 60 F254, Merck) and compounds were visualised by UV radiation at 254 nm and then using an anisaldehyde spray reagent (1% p-anisaldehyde:2% H2SO4 ...
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bioRxiv - Microbiology 2024Quote: Extracted lipid (dried organic mixture) was solubilized in chloroform and 4µg was resolved by 2D TLC on aluminum-backed silica gel plates (Merck, India) by spotting the extract using microcapillary pipettes as described Pirson et al. ...
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bioRxiv - Cell Biology 2024Quote: Samples were resuspended in 6 μL 40% (v/v) 1-propanol with vortexing and bath sonication were applied to Silica Gel 60 aluminium-backed HPTLC plates (MERCK) (30 ...
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bioRxiv - Neuroscience 2024Quote: ... Neurons were collected in Eppendorf tubes by disrupting adhesion to the plate with a jet of medium and washed twice in ice-cold PBS with Ca2+ and Mg2+ (Merck), supplemented with complete ...
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Insight into the regulatory mechanism of the MFS transporter, SCO4121 by the MarR regulator, SCO4122bioRxiv - Molecular Biology 2024Quote: ... the MSA agar plate was flooded and 500 µg/ml of nalidixic acid (Himedia) and 1 mg/ml of apramycin (Merck). After incubation ...
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bioRxiv - Cell Biology 2023Quote: For the thin layer chromatography (TCL) an aliquot of lipid extract corresponding to 8 mg of DCW was applied to silica gel TLC plates (Merck) by a Linomat 5 semiautomatic sample applicator (Camag) ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were monitored by thin-layer chromatography on commercially available silica pre-coated aluminium-backed plates (Merck Kieselgel 60 F254). Visualisation was under UV light (254 nm and 366 nm) ...
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bioRxiv - Immunology 2023Quote: ... Cells were transferred to plates, and then washed in 100-200 μL FACS buffer (PBS containing 2% FCS, 2 mM EDTA (Merck) and centrifuged at 400 x g for 5 mins ...
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bioRxiv - Plant Biology 2023Quote: ... Cell debris was removed with centrifugation and 2+2 μl of extracts and a commercial pinosylvin standard (ArboNova) were applied on TLC plates Silica gel 60 F254 (Merck), which were developed with chloroform ...