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Citations for Merck :
551 - 600 of 4834 citations for T complex protein 1 subunit epsilon CCT5 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... 2.0 Protein Detection System (Merck Millipore), incubated with Clarity Western ECL (BioRad) ...
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bioRxiv - Immunology 2024Quote: ... Proteins were alkylated with iodoacetamide (Merck) and sequentially digested on the filter with LysC (Wako ...
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bioRxiv - Microbiology 2024Quote: ... Protein A-Sepharose beads from Merck-Cytiva (USA) ...
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bioRxiv - Biophysics 2024Quote: ... Primary anti-KI67 antibody (Merck, rabbit polyclonal antibody) was diluted 1:300 in antibody buffer (0.2 % Tween 20 ...
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bioRxiv - Microbiology 2022Quote: Non-infected and MCMV-infected mice were orally gavaged with 150 μl of 100 mg/ml 4 kDa FITC-dextran-labeled dextran beads (Sigma-Aldrich/Merck, Darmstadt, Germany) in PBS 4 hours prior to sacrifice ...
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bioRxiv - Microbiology 2022Quote: ... mice were gavaged with 0.2 ml of Fluorescein Isothiocyanate-Dextran (FITC-Dextran; 3–5 kDa; cat. no. FD4; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were used: mouse monoclonal anti-SARM1 (Chen et al., 2011) (1:5000) and mouse anti-β-actin (Merck, A5316, 1:2000) as a loading control ...
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bioRxiv - Biochemistry 2023Quote: In situ protein-protein interaction was evidenced using Duolink® Proximity Ligation Assay (Merck, DUO920082, DUO92004, DUO92008). Cells were fixed with formaldehyde and permeabilized using ice-cold 90% methanol ...
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bioRxiv - Developmental Biology 2022Quote: ... immunofluorescence staining was performed using overnight incubation with polyclonal anti-Prosurfactant Protein C (ProSP-C) (Merck/Millipore/Sigma Aldrich, 1:500) followed by staining with polyclonal secondary antibody Goat anti-rabbit Alexa fluor 488 (Invitrogen,1:500) ...
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bioRxiv - Biochemistry 2020Quote: ... epithelium suspension cell line expressing the EBNA-1 protein (female origin) was cultured in EX-CELL(r) 293 Serum-Free Medium (Merck, 14571C) containing 4 mM GlutaMAX supplement (TermoFisher ...
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bioRxiv - Biochemistry 2024Quote: ... The purified protein sample was concentrated to 40 mg mL-1 using Amicon Ultra-15 3K MWCO Centrifugal Filter Unit (Merck Millipore) and subsequently diluted 1:4 with MilliQ water (yielding 10 mg mL-1 of protein in 1x crystal buffer (10mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... and the protein of interest was concentrated to 1-2mg/ml in a 50kDa molecular weight cut-off concentrator (Amicon Ultracel, Merck-Millipore). The resulting protein solution was subjected to size exclusion chromatography on a Superose 6 column (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were pre-cleared at 4°C for 1 h on a rotating wheel using 30 µl Protein-S agarose beads (Merck Millipore) slurry ...
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bioRxiv - Cell Biology 2023Quote: ... For fluorescent imaging the 30 µM protein solutions were supplemented with 300 nM (100:1 molar ratio) NTA-Atto 550 (Sigma Aldrich, Merck). Movies were acquired at 37°C with a PerkinElmer UltraVIEW VoX microscope equipped with Yokogawa CSU-X1 spinning disk head and Nikon TiE microscope body with an Apo 60x/1.49 numerical aperture (NA ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 μl of Protein powder solutions (1 mg/ml) were added to 3 cm NG plates with 10 µM 5-FU (Merck, #F6627). After the plates had dried ...
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bioRxiv - Molecular Biology 2022Quote: ... CD3+ T cells were cultured in TexMACS Medium (Miltenyi Biotech) supplemented with 5% heat-inactivated human AB serum (Merck), 100 units/mL penicillin (Merck ...
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bioRxiv - Developmental Biology 2023Quote: ... the HCR protocol involved rehydrating the gastruloids with a descending series of methanol concentrations and finally PBS-T (0.1% Tween, 655204, Merck). Once hydrated ...
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bioRxiv - Molecular Biology 2020Quote: ... The membranes were probed with a secondary antibody: either anti-rabbit-HRP (1:5,000; Merck Millipore AP132P), anti-mouse-HRP (1:5,000 ...
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bioRxiv - Genomics 2020Quote: ... incubated with primary antibodies (anti-AP-1 Thermo Scientific™ #MA5-15172, anti-Sp1 ChIPAb+™ Merck #17-601 ...
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bioRxiv - Cell Biology 2020Quote: ... or HRP-conjugated monoclonal anti β-actin antibody (1:20,000, Sigma-Aldrich, Merck, St. Louis, Missouri, USA) for a loading control ...
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bioRxiv - Molecular Biology 2022Quote: ... Acetylation was evaluated with Western blot probed with anti-acetyl lysine antibodies (1:800 dilution) (Sigma/Merck). Mass spectrometry outsourced to Mass Spectrometry Laboratory IBB PAN ...
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bioRxiv - Neuroscience 2021Quote: ... Primary and secondary antibodies used were: monoclonal mouse anti-puromycin (clone 12D10, MABE343, Merck Millipore; 1:1000), polyclonal rabbit anti-Tau (T6402 ...
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bioRxiv - Neuroscience 2020Quote: ... samples were incubated overnight at 4°C with primary antibodies against puromycin (1:500, MABE343, Merck Millipore), βIII tubulin (1:500 ...
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bioRxiv - Neuroscience 2020Quote: The following primary antibodies were used: rabbit polyclonal anti-MuSK (1:1,000; Merck Millipore, catalog no. ABS549), anti-HA.11 (1:100 for immunoprecipitation ...
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bioRxiv - Cell Biology 2020Quote: ... c-Src was detected using anti-Src (GD11 clone) antibody (1:200, Merck Millipore, Mouse, 05-184). Phosphorylated c-Src was assessed using anti-phospho-Src Tyr 418 antibody (1:100 ...
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bioRxiv - Developmental Biology 2022Quote: ... The primary antibodies and working dilutions used in this study were: SOX9 (Merck millipore, AB5535, 1:600), FOXL2 (abcam ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were immunolabelled with anti-phospho-Histone H3 (Ser10) primary antibody (1:500, 06-570 Merck millipore), washed for 0.5 day in PBS/0.1% Tween-20/10μg/ml heparin (PBSTwH ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... cells were incubated with an IF-optimized EMRE antibody (anti-SMDT1/C22orf32/EMRE; #HPA060340, 1:250; Merck), washed 3 times in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated with the secondary antibodies (Alexa 647-conjugated donkey anti-guinea pig, 1:200, AP193SA6, Merck Chemicals ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated with the secondary antibodies (Alexa 647-conjugated donkey anti-guinea pig, 1:200, AP193SA6, Merck Chemicals ...
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bioRxiv - Developmental Biology 2023Quote: ... gastruloids were incubated with secondary antibodies and 4’,6-diamidino-2-phenylindole (DAPI, 1 µg/mL, Merck) in OWB-SDS at 4°C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... and secondary antibody anti-rabbit (CST, 1:3000) diluted in PBS containing 4% skim-milk powder (Merck) and 0.2% Tween 20 (Sigma).
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bioRxiv - Molecular Biology 2024Quote: ... The following antibodies were used for the nChIP reactions: rabbit mAbs α-H3K4me3 (1:200; Merck Millipore), α-H3K27me3 (1:300 ...
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bioRxiv - Biochemistry 2024Quote: ... wells were incubated with the NCAM specific monoclonal antibody OB11 (Merck, Cat.# C9672, mouse IgG1; 1:1000), followed by horseradish peroxidase (HRP)-conjugated anti-mouse IgG1 (Jackson Cat.# 115-035-205 ...
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bioRxiv - Cell Biology 2024Quote: ... or with mouse monoclonal anti-α-tubulin antibody (1:1000; T6199; Sigma Aldrich; Merck KGaA, Darmstadt, Germany) in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated in an anti-tyrosine hydroxylase (TH) primary antibody (Merck Millipore, USA, #AB152, 1:5000) at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... This involved washing the antibody in 1× PBS using Amicon ultra 30K 0.5mL columns (UFC5030, Merck GmbH), followed by collection of the purified antibody and determination of its concentration using a NanoDrop spectrophotometer at 280 nm.
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bioRxiv - Plant Biology 2024Quote: ... before and after incubation of blots with appropriate secondary antibody (anti-rabbit HRP (Merck #A0545; 1:50,000); anti-mouse HRP (Merck #A9044 ...
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bioRxiv - Biophysics 2024Quote: The following primary antibodies were used in this study: anti-Myc (clone 4A6, Merck KGaA, 1:1000), anti-HA (goat polyclonal ...
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bioRxiv - Biochemistry 2024Quote: ... yeast cells were resuspended in 1:10-diluted primary mouse anti-cMyc antibody (#OP10, EMD Millipore, Merck) in PBS (50 μL ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies: anti-LC3 antibody Atg8 (Merck Millipore #ABC975) (1:1000) ...
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bioRxiv - Microbiology 2022Quote: ... At least 5,000 cells were counted per run and FITC-positive cells were detected with a FlowSight imaging flow cytometer (Amins, Merck Millipore, Billerica, MA, USA) equipped with lasers at 405 nm (violet) ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were incubated for 1 hour with HRP-conjugated secondary antibodies (1:5000, anti-mouse, Sigma-Aldrich Catalogue No A9044; Flag, or 1:10000, anti-rabbit, Merck, Catalogue No GENA934; LpoA) in TBS-TM ...
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bioRxiv - Neuroscience 2024Quote: ... sections were blocked in 2 % goat or donkey anti-serum in KPBS and 3 % BSA plus 0.1 % Triton X-100 and incubated with primary antibody (rabbit anti-c-Fos, 1:200, Cell Signaling, Danvers, MA, USA; sheep anti-αMSH, 1:1000, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) for 1 h at room temperature or overnight at 4 °C ...
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bioRxiv - Genomics 2020Quote: ... The protein solutions were subsequently desalted and protein amount was estimated using Direct Detect cards (Merck Millipore, Darmstadt). All samples were freeze dried before further processing ...
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bioRxiv - Cell Biology 2023Quote: Protein carbonyl formation by oxidative reactions was assessed with the OxyBlot Protein Oxidation Detection Kit (S7150, Merck Millipore), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin pre-clearing was done by incubating with Protein G Plus/Protein A Agarose beads (Merck Millipore #IP05) for 4 hrs at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... The pellets were lysed for 1 h using cell protein extraction reagent (CW BioTech, CW0889, China) with protease inhibitor Cocktail set III (1 %, v/v, Merck, 535140, Germany), followed by centrifugation at 500×g for 10 min and the supernatants were collected ...
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... and eSpCas9-GFP protein (#ECAS9GFPPR, Merck KGaA) were introduced into MCF7 and T47D cells using Viromer® CRISPR (Lipocalyx GmbH ...
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bioRxiv - Evolutionary Biology 2020Quote: ... protein G-magnetic beads (Merck Millipore, LSKMAGG10) were used instead ...