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Citations for Merck :
551 - 600 of 624 citations for Saporin Goat Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... They were incubated 1h at RT in a blocking buffer (20% BR + 20% goat serum) and then overnight with an anti-DIG-AP antibody (1:2000, Merck) in the blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... slides were blocked for 30 min in 0.3 % Triton / 10 % normal goat serum / TBS followed by overnight primary antibody incubation (1:1000; rb TH; Merck Millipore) at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then placed in 10% horse serum/PB for 1 h before being incubated in primary goat anti-ChAT (1:500; #AB144P, Merck) for 2 d at 4°C to aid identification of CINs ...
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bioRxiv - Pathology 2023Quote: ... Then sections were blocked for 45 min using a blocking buffer containing 5% goat or donkey serum (depending on the host species of the secondary antibody) (#G9023 and #D9663, Merck) and 0.3M glycine (#56-40-6 ...
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bioRxiv - Neuroscience 2023Quote: ... this was followed by the incubation in the blocking solution containing secondary antibody anti-Rabbit or/and goat IgG antibody conjugated with HRP (GENA934, AP106P, Merck) at room temperature for 1 hour for enhanced chemiluminescence ...
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bioRxiv - Biophysics 2022Quote: ... and monomeric protein was purified by size-exclusion chromatography using a HiLoad™ 26/600 Superdex™ 75 pg (Merck) equilibrated with 25 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified by polyethylene glycol and ethanol precipitation and were pooled and concentrated using Amicon 0.5ml 50K columns (Merck, Germany). Amplicon sizes were checked using TapeStation (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: Polar metabolites were first extracted from the frozen SHIME-samples by means of ultra pure water (0,055 μS cm-1) obtained via a purified water system (VWR International, Merck, Germany). For this purpose ...
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bioRxiv - Microbiology 2021Quote: 2mL SARS-CoV-2 P4 supernatant containing 2 x 107 pfu/mL was purified using an Amicon Ultra column (MWCO 100kDa; Merck) by centrifugation and washing in PBS at 2,000 x g ...
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bioRxiv - Plant Biology 2021Quote: ... Purified ClpP complexes were concentrated to ~2 mg/ml by Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore, Beijing China) with 100 kDa cut-off ...
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bioRxiv - Cell Biology 2020Quote: ... Cell medium was replaced with 3 mL medium containing 20 μL of the purified lentivirus and 3μl polybrene transfection reagent (Merck Millipore). Medium was supplemented with 10 µg/mL puromycin for selection of successfully transduced cells two to three days after transduction.
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bioRxiv - Biochemistry 2022Quote: ... Purified ShTniQ was concentrated to 10 mg mL−1 using 10,000 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen ...
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bioRxiv - Developmental Biology 2020Quote: Women were stimulated with highly purified urinary hMG (Menopur®, Ferring, Spain) or follitropin alpha (Gonal®, Merck-Serono, Spain), with daily injections of 150-300 IU (Blazquez et al. ...
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bioRxiv - Immunology 2021Quote: ... and filtered through a 0.22 µM membrane The concentration of purified RBD was determined using the Bradford assay (Merck, Germany).
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bioRxiv - Immunology 2020Quote: ... MS2-FP8 VLPs were concentrated from SEC purified fractions by ultra-centrifugal filtration using Amicon® Ultra-15.0mL 100 K membrane (Merck Millipore Ltd. ...
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bioRxiv - Biochemistry 2021Quote: ... Purified ShTnsC was concentrated to 1-2 mg mL−1 using 30,000 molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... The glutathione-S-transferases (GST) fusion proteins were expressed in Escherichia coli and purified with glutathione-conjugated agarose beads (Merck) by standard procedures ...
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bioRxiv - Neuroscience 2021Quote: 400 μg of purified GABAAR mAbs were concentrated to a volume of 100 μl using Amicon Ultra 100 kDa columns (Merck) and then incubated with 40 μg CruzFluor™ 488 (CF488 ...
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bioRxiv - Immunology 2022Quote: For protein crystallization “empty” HLA-DPA1*02:01-DPB1*01:01 was purified on a Superdex 75 10/300 GL column (Merck) into 20mM Tris pH 8.0 ...
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bioRxiv - Immunology 2023Quote: ... The tetramer (428 kDa) was purified from excess GFP-biotin (25 kDa) with a 100 kDa cut-off Amicon Ultra centrifugal filter (Merck). Functionalised agarose beads were washed twice and then incubated with 20 nM of the GFP-streptavidin tetramers and anti-mouse IgG2a/b BV421 antibody (1:10 dilution ...
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bioRxiv - Biophysics 2023Quote: ... The purified Nup98 was concentrated to a final concentration of 165 µM using 3-kDa MWCO centrifugal filters (Merck Millipore) in a solution of 2 M GdmCl ...
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bioRxiv - Biochemistry 2023Quote: ... The purified samples were concentrated with Vivaspin centrifugal concentrators with cut-off corresponding to the considered fragments (Merck Millipore Corporation) to a volume of 500 μL and exchanged with a NAP-5 column (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2022Quote: ... 9.6 grams of the powdered reagent was added to 990 milliliters of ultra-purified milli-Q water (Merck, Millipore systems). A pH of 7.4 was maintained using 1 N Hydrochloric acid (HCl ...
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bioRxiv - Physiology 2023Quote: ... using the 5x QPCR Mix EvaGreen (No Rox) (Solis Biodyne, Cat. # 08-25-00001-20, Estonia) and purified water (Merck Millipore ...
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bioRxiv - Physiology 2023Quote: ... using the 5x QPCR Mix EvaGreen (No Rox) (Solis Biodyne, Cat. # 08-25-00001-20, Estonia) and purified water (Merck Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged Trx1 was purified on a Ni2+ sepharose resin following the manufactureŕs instructions (cOmplete™ His-Tag Purification Resin, Merck).
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bioRxiv - Plant Biology 2024Quote: ... the purified protein was concentrated by centrifugation in 3 kDa cut-off filters (Amicon Ultra-15 Centrifugal Filter Units, Merck) at 4,500 x g ...
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bioRxiv - Plant Biology 2024Quote: ... The purified protein was concentrated by centrifugation in 3 kDa cut-off filters (Amicon Ultra-15 Centrifugal Filter Units, Merck) at 4500 × g ...
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bioRxiv - Plant Biology 2024Quote: ... Apoenzyme preparations of D-AAT and AtPLPHP1 were achieved by incubating 100 μM of purified protein with 75 mM D-cycloserine (Merck) in 50 mM HEPES pH 7.4 containing 300 mM sodium chloride on ice for 2 hours followed by buffer exchange as described above ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The PCR products were gel purified using MSB Spin PCRapace (Invitek Molecular) and an in vitro transcription was performed using T7 Polymerase (Merck). The remaining DNA was then degraded using DNAse I (Life Technologies) ...
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bioRxiv - Plant Biology 2024Quote: ... the purified protein was concentrated by centrifugation in 3 kDa cut-off filters (Amicon Ultra-15 Centrifugal Filter Units, Merck) at 4,500 x g ...
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bioRxiv - Microbiology 2024Quote: ... individually rinsed in aseptic conditions and placed in enclosed sterile polycarbonate water tanks (AquaMedic, Germany) with 2.5 L of ultra purified water (Mili-Q, Merck, Germany) each ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The PCR products were gel purified using MSB Spin PCRapace (Invitek Molecular) and an in vitro transcription was performed using T7 Polymerase (Merck). The remaining DNA was then degraded using DNAse I (Life Technologies) ...
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bioRxiv - Biochemistry 2024Quote: ... The purified solutions were concentrated with an Amicon Ultra-15 Centrifugal Filter (NMWL of 10 kDa, Merck Millipore, Burlington, USA). For protein concentration determination ...
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bioRxiv - Biophysics 2021Quote: ... The antibody was revealed using Atto 594 goat anti-mouse IgG (1:500, 76085-1ML-F, Merck & Co., Kenilworth, NJ, USA). The coverslips were rinsed in PBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the slides were incubated for 1 h at room temperature with the appropriate biotin-conjugated secondary antibody: goat anti-mouse (21538, Merck-Millipore) for CD3 and goat anti-rat (BP-9400 ...
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bioRxiv - Biochemistry 2020Quote: ... cells were washed 3x in PBS followed by blocking in 16% (v/v) normal goat serum (Merck Milipore, Burlington, MA, USA) supplemented with 0.1% (v/v ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 2 h with the proper secondary antibodies (1:300, goat anti-rabbit Alexa-Fluor® 647, AP187SA6, Merck Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... After washing, the bound nanobody was detected by HRP-conjugated Goat anti-Alpaca IgG (Cat. S001P, NBbiolab) using Tetramethylbenzidine (TMB) (Merck, Cat.T2885) as a substrate for HRP ...
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bioRxiv - Neuroscience 2022Quote: ... brain sections were blocked with 5% normal donkey serum for 1 h at room temperature and incubated with primary antibodies (rabbit anti-GFAP 1:200, Dako; goat anti-Iba1, 1:500 Wako; NeuroTrace™ 1:200, Thermo Fischer; mouse anti-NeuN Antibody 1:500, Merck, #MAB377 ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated with a BG4-Flag antibody (1:100 diluted in 0.5% goat serum + PBST, MABE917, Merck, Darmstadt, Germany) for 1 h at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... samples were washed in PBS for 3 times and Alexa Fluor 555 conjugated secondary goat anti-mouse IgG antibody (1:100; Merck, Germany) was added for immunostaining in the dark for 2 hours at RT ...
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bioRxiv - Neuroscience 2023Quote: ... R&D, AF2408), Goat-Anti Human SOX17 (1:100, R&D, AF1924) or Mouse Anti-Human Beta-Tubulin (1:1000, Merck, T8660) primary Chicken Anti-Human MAP2 (1:2000 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the PCR fragment was amplified with the primers detailed in Supplementary Table 1 and purified in MultiScreen TM Vacuum Manifold 96-well plates (Merck Millipore). The purified PCR fragment was incubated with either the enzyme NlaIII or water (negative control ...
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bioRxiv - Neuroscience 2021Quote: ... the AAV-containing medium was collected and purified from cell debris using a 0.45 μm Millex-HV syringe filter (SLHV033RS, Merck Millipore, Germany). The filtered medium was concentrated and diluted with D-PBS (14190-144 ...
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bioRxiv - Neuroscience 2021Quote: ... the AAV-containing medium was collected and purified from cell debris using a 0.45 μm Millex-HV syringe filter (SLHV033RS, Merck Millipore, Germany). The filtered medium was concentrated and diluted with D-PBS (14190-144 ...
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bioRxiv - Microbiology 2020Quote: ... The purified fractions were subjected to desalting and concentration using an Amicon Ultra centrifugal filter unit (30 kDa cutoff; Merck Millipore), and the enzyme preparations were stored at −80°C ...
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bioRxiv - Plant Biology 2021Quote: ... Each exudate sample was purified using solid phase extraction (SPE) with C18 Discovery® cartridges (bed wt. 500 mg, volume 6 mL, Merck). Cartridges were activated using 5 mL acetone and washed with 5 mL distilled water ...
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bioRxiv - Microbiology 2020Quote: The crude extract and purified fractions were separated on TLC Silica gel 60 F254 plates (Merck Life Science Pvt. Ltd., Germany) using the solvent system of hexane ...
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bioRxiv - Microbiology 2020Quote: ... Purified DesX was desalted and concentrated by centrifugal filtration using an Amicon Ultra centrifugal filter unit (10 kDa cutoff; Merck Millipore). Before the assay ...