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Citations for Merck :
551 - 600 of 3486 citations for Rat Translational Activator Of Cytochrome C Oxidase 1 TACO1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... with PBS pH 7.4 for 10 min at 4400 x g and 4°C using an Amicon Ultra-4 concentrator with 3 kDa cutoff (Merck Millipore). The degree of labelling (DOL = 1.96 ...
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bioRxiv - Neuroscience 2022Quote: ... Unencapsulated molecules were removed by washing at least 4 times the LPs suspensions with PBS pH 7.4 for 20 min at 4°C and 5000 x g using an Amicon Ultra-4 concentrator with 100 kDa cutoff (Merck Millipore). The degree of encapsulation was calculated by subtracting from the total mass of the molecule to be encapsulated that present in the flow-through and was found to be higher than 75% in all LP preparations.
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bioRxiv - Microbiology 2023Quote: ... bacteria were freshly grown for 20 to 24 h on plates in anaerobic jars supplemented with Anaerocult C sachets (Merck, USA) at 37°C ...
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bioRxiv - Immunology 2023Quote: ... the supernatant was concentrated by centrifugation at 1000 g at 4 °C through Amicon® Ultra 15 mL Centrifugal Filters (100 K; Merck). Finally ...
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bioRxiv - Biochemistry 2023Quote: ... Purified Mpro with authentic N- and C-termini was concentrated by a 10 kDa MWCO Amicon Ultra 50 centrifugal filters (Merck Millipore) at 4 °C to ∼ 10 mg/ml ...
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bioRxiv - Biochemistry 2023Quote: All peptides used in this study probes were designed as 11-mers bearing biotinylated PEG2 linker in the N terminus and an amide group in the C terminus and were synthesized using Fmoc-solid-phase peptide chemistry with Novasyn TGR resin (Merck Millipore) as described in the Novabiochem Innovations 01/11 ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were grown in 25% TSB medium at 28°C in static conditions in glass microcosms with one layer of ColiRoller glass beads (Millipore, Merck, USA) covering the base of the microcosm to provide additional spatial structure ...
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bioRxiv - Neuroscience 2023Quote: ... Separated proteins were wet-blotted (110 V; 90 min; 4°C) on fluorescence-optimized polyvinylidene difluoride (PVDF) membranes (Immobilon-FL; Merck; IPFL00010) in transfer buffer (25 mM Tris (pH 7.6) ...
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bioRxiv - Plant Biology 2023Quote: ... then 200 µl of each sample was digested for 16 h at 37 °C with α-amyloglucosidase (6 U/ml, Merck), and α-amylase (4 U/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatants were transferred to new tubes and incubated overnight at 4 °C with 15 µl of Anti-FLAG® M2 Magnetic Beads (Merck) with gentle mixing ...
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bioRxiv - Biochemistry 2023Quote: ... the Streptag-purified protein after removal of the C-terminal affinity-GFP-tag was collected and concentrated using 100 kDa MW cut-off spin concentrator (Amicon Merck-Millipore) further purified by size-exclusion chromatography (SEC) ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore).
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bioRxiv - Cell Biology 2020Quote: Cellular Senescence Assay kit (Merck Millipore: KAA002) was used to detect senescent AML12 by SA β-Gal staining as per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... with a customized Milliplex kit (Merck Millipore).
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bioRxiv - Molecular Biology 2022Quote: ... and GenElute™ Plasmid Miniprep Kit (Merck), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... we used Sigma-Aldrich kit from Merck-Sigma ...
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bioRxiv - Immunology 2023Quote: ... using Mouse Kapa Genotyping kit (Merck-Millipore), primers (500 nM ...
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bioRxiv - Microbiology 2023Quote: ... Nutrient specific Spectroquant kit (Merck Millipore, US) was used and the concentration was determined spectrophotometrically using a 96-well plate with a microplate reader (FilterMax F5 ...
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bioRxiv - Molecular Biology 2024Quote: Magna MeRIPTM m6A Kit (17-10499, Merck) was used to enable identification and transcriptome-wide profiling of m6A ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 μl MTT assay kit reagent (Merck) was then added to each well ...
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bioRxiv - Genomics 2023Quote: ... Primary antibodies (FUS 1:100 Santa Cruz Biotech sc-47711; ISL1 1:500 Abcam ab109517; OLIG2 1:100 R&D Systems AF2418; NFM 1:1000 Merck MAB1621) were added in 1% BSA and incubated at 4degC overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... Tubulin beta (1:8,000; clone KMX-1 from Merck Millipore), dMi-2 (1:8,000 ...
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bioRxiv - Neuroscience 2019Quote: ... anti-vGlut (Merck Millipore, cat MAB5502, 1:200; 1:1,000); anti-GluR2 (Merck Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... and anti-TUJ-1 antibodies (1:200; Merck-Millipore, MAB380) for 48 h ...
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bioRxiv - Cell Biology 2021Quote: ... Armenian hamster PECAM-1 (MAB1398Z, Merck Millipore, IHC: 1/500); goat VE-cadherin (sc-6458 ...
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bioRxiv - Systems Biology 2023Quote: ... after adding 1 mL of chloroform:methanol 2:1 (v:v) (Merck), the sample was vortexed at 1200 rpm for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and 1 μL RNase A (10 U µL−1) (Merck) per mL of stock and incubated at 37°C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg ml−1 collagenase IV (Merck Millipore, C4-22) and 70 U ml−1 DNase (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... Immuno-identification of the samples was done using α-c-Myc rabbit antibody and followed by a secondary HRP-conjugated α-rabbit antibody (Merck). α-Histone H3 rabbit antibody was used as housekeeping protein ...
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bioRxiv - Microbiology 2020Quote: ... Expanded and chopped gels were stored at 4°C in ddH2O immobilized prior to imaging on PDL-coated glass chambers (Merck, 734-2055).
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bioRxiv - Cell Biology 2020Quote: ... for 3h at 16°C to remove the 6-His and MBP tag before phase separation in reaction mixtures in buffer C containing 10μM of WT or W1145R TopBP1b6-8-GFP and 2% of PEG4000 (Merck-Sigma-Aldrich, 95904). Reaction mixtures were mixed by gently tapping the Eppendorf ...
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bioRxiv - Cell Biology 2020Quote: U-2-OS Tet-On cell lines expressing eGFP-TopBP1 WT were grown under standard conditions (37°C, 5% CO2) in modified McCoy’s 5a medium (Merck-Sigma-Aldrich, M9309) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2021Quote: ... and subsequent thorough rinsing with ultrapure water (18.2 MΩ at 25 °C, maximum 2 ppb total organic carbon, Milli-Q Integral 5 Water Purification System, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Cell Biology 2022Quote: ... It was inserted with N and C-terminal tags (His6 and StrepII, respectively) in the baculovirus transfer vector pIEX/Bac3 (Merck Novagen #717263).
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bioRxiv - Neuroscience 2020Quote: ... The cortices were homogenized mechanically in PBS by pipetting up and down with a 5mL plastic pipette, centrifuged (500 x g, 7 min, 4 °C) and resuspended in DMEM (Sigma-Aldrich, Merck, Switzerland) containing 5% FCS (Biochrom ...
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bioRxiv - Cell Biology 2022Quote: ... Ovaries were dissected and oocytes recovered by mouth- pipetting follicles through a narrow glass pipet.Prophase I oocytes were cultured at 38° C in drops of M2 or M16 medium (homemade) supplemented with dbcAMP (dibutyryl cyclic AMP, Sigma Aldrich Merck, D0260) and covered with mineral oil (Sigma-Aldrich Merck ...
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bioRxiv - Physiology 2019Quote: ... Individual gel slices were subjected to in-gel reduction with dithiothreitol (10 mM, 30 min, 56 °C, Merck-Calbiochem, San Diego, USA), alkylation with iodoacetamide (50 mM ...
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bioRxiv - Microbiology 2020Quote: ... water was purified by a Milli-Q system (resistivity 18.2 MΩ cm at 25°C, TOC < 5 ppb, Merck Millipore, Molsheim, France). Indole-3-acetic-2,2-d2 acid (≥ 98% ...
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged (16,000 g, 10 min, 4°C) and supernatants measured for protein content using infrared spectroscopy (Merck Millipore, Darmstadt, Germany). Lysates were then reduced ...
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bioRxiv - Molecular Biology 2021Quote: ... mESCs were grown on gelatin-coated plates under standard culture conditions (37°C, 5% CO2, humid) atop a ‘feeder’ layer of MitomycinC-inactivated (Merck Life Science) SNLP mouse fibroblasts and passaged upon ∼80% confluency every 2-3 days using TrypLE Express (ThermoFisher ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA fragment encoding mouse C/EBPβ residues 221–296 (bZIP domain) was cloned into pET-45b(+) vector (Novagen/Merck, Darmstadt, Germay) via ‘in vivo assembly’ (35 ...
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bioRxiv - Molecular Biology 2021Quote: ... The prepared sections on glass slides were deparaffinized for 30 minutes at 65°C and then for 2x 30 minutes in Neo-Clear (Merck Millipore, 109843). Samples were rehydrated for 2x 10 minutes in 100% ethanol ...
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bioRxiv - Genomics 2021Quote: ... Each lobe was segmented so cryosections would fit on the 6,200 x 6,400 µm areas of the Codelink-activated microscope slides and frozen in −30°C 2-Methylbutane (Merck, cat.no.: M32631-1L). The frozen liver samples were embedded in cryomolds (10×10 mm ...
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bioRxiv - Cancer Biology 2021Quote: ... Polar metabolites were derivatized for 90 min at 37 °C with 20 μl of 20 mg per ml methoxyamine (Merck Sigma, 226904) in pyridine (Merck Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Water was purified by a Milli-Q system (resistivity 18.2 MΩ cm at 25°C, TOC < 5 ppb, Merck Millipore, Molsheim, France). For laboratory culture samples ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated 48 hours at 37°C with BrdU (15 μM, stock solution 30 mM in H2O) (Merck, Darmstadt, Germany). After washing two times with warm PBS the cells were pre-extracted with ice cold NuEx buffer and fixed with 2% (w/v ...
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bioRxiv - Physiology 2022Quote: ... and separation was carried out at 40°C using a ZIC-HILIC column (2.1 mm × 100 mm, 3 μm; Merck Sequant, Umea, Sweden). Isocratic elution was performed ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were subjected to sonication on ice (10 cycles consisting of 10-s impulses at an output 10% followed by 20-s intervals) followed by incubation for 20 min at 37 °C in the presence of micrococcal nuclease (2 units/sample; Merck, cat. # N3755) and CaCl2 (at 10 mM) ...
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bioRxiv - Cancer Biology 2022Quote: ... (MW 360 kDa) and (Hydroxypropyl)-methylcellulose (HPMC) (viscosity 40-60 cP, 2 % in H2O (20 C) were purchased from Merck (Darmstadt, Germany). Methanol ...