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Citations for Merck :
551 - 600 of 2435 citations for Inactive tyrosine protein kinase 7 PTK7 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... the proteins were transferred onto PVDF membrane (Merck Millipore ...
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bioRxiv - Synthetic Biology 2022Quote: ... Chemicals and protein reagents were purchased from Merck, Sigma ...
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bioRxiv - Immunology 2022Quote: ... Proteins were transferred to PVDF membrane (#IPFL00010, Merck) for 30 minutes at 100 V with Tris/Boric buffer at 50 mM/50 mM ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were blotted to a PVDF membrane (Merck) for 1-2 h at 4°C with 300 mA constant current using the Bio-Rad immunoblot apparatus ...
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bioRxiv - Pathology 2024Quote: ... a HA binding protein (HABP, Merck, cat.number 385911) with a biotin conjugate was used (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to PVDF membranes (MERCK-Millipore) using the Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Recombinant active DAPK3 protein was obtained from Merck.
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred onto PVDF membranes (Merck Millipore). The membranes were blocked with skim milk ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein was transferred onto a PVDF membrane (Merck) at 60 V for 90 min in 1× NuPAGE Transfer Buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to PVDF membranes (Merck, IPFL00010) by semi-dry blotting ...
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bioRxiv - Developmental Biology 2024Quote: ... the protein was incubated with HRV3C protease (Merck) while dialysed against a size exclusion buffer (20 mM Hepes pH7.2 ...
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bioRxiv - Microbiology 2024Quote: ... After measuring the protein concentrations (Bradford reagent, Merck), 200 µg samples were alkylated with 45 mM iodoacetamide (IAA ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were concentrated with either Aquacide II (Merck) or Amicon® Ultra Centrifugal Filters ...
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bioRxiv - Biophysics 2024Quote: ... NavMs proteins were treated with thrombin protease (Merck) for 16 h at 4°C then purified by size exclusion chromatography using a Superdex 200 10/300 column (Cytiva Life Sciences) ...
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bioRxiv - Immunology 2022Quote: ... the cells were transferred to a single well containing a monolayer of mitomycin C (Cat no. 50-07-7, Millipore Sigma, Merck, Darmstadt, Germany)-treated OP9/ OP9-DL1 cells or were kept unaltered ...
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bioRxiv - Biochemistry 2021Quote: ... a biotinylated DNA oligonucleotide complementary to the target tRNA (Supplementary Table 7) was immobilized on Novagen Streptavidin Agarose resin (Merck-Millipore, Burlington, MA) in a 0.22 μm Ultrafree-MC filter cup (Merck-Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 mM EDTA) with 1 µl lyticase (17 U/ µl in 10 mM KPO4 pH 7, 50% glycerol, Merck >2000 U/mg L2524), heated to 50°C for 2 min before addition of 40 µl molten CleanCut agarose (Bio-Rad 1703594) ...
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bioRxiv - Biochemistry 2024Quote: Mixing of lysozyme crystals (7×2 mm; grown by batch crystallisation) with 25 mM sulfanilic acid azochromotrop (SAA, Merck, lmax 505–510 nm), a highly absorbing red dye ...
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bioRxiv - Molecular Biology 2022Quote: ... was placed on the top of a water-cooled glass column (33 × 2.5 cm) filled with a slurry of silica gel 60 (with the addition of 7 % water, 40 – 63 μm, Merck, Darmstadt, Germany, # 1.09385.2500) and n-pentane ...
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bioRxiv - Bioengineering 2024Quote: ... was assessed every day for 7 to 10 days using a Millicell ERS-2 voltammeter EVOM2 and an STX02 chopstick electrode (Merck Millipore, Bayswater Australia). The TEER value was calculated by subtracting the resistance of a blank insert from an insert containing a monolayer and then multiplying it by the surface area of the insert.
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bioRxiv - Developmental Biology 2020Quote: ... Fifty or thirty-five (depending on the protein detection) micrograms of proteins was separated on 9% Tricine gels and transferred to polyvinylidene difluoride membranes (Merck Millipore). The membranes were incubated separately with anti-Mmp-9 (1:1000 ...
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bioRxiv - Plant Biology 2023Quote: ... The separated total proteins or proteins from two-dimensional gels were subsequently transferred onto polyvinylidene difluoride (PVDF) membranes (Merck, Cat. IPVH00010), hybridized with specific antibodies ...
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bioRxiv - Synthetic Biology 2024Quote: ... Cell debris was removed via centrifugation and the protein concentration in the supernatant determined utilizing the BCA-Protein-Assaykit (Merck, Germany) according to the manufacturers instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated with the primary antibodies (see the list of antibodies in Table S2) and with an anti-rabbit secondary antibody (Merck), and visualized by chemiluminescence substrate (Merck ...
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bioRxiv - Neuroscience 2022Quote: ... antibodies (α-GluA2, Merck clone 6C4 ...
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bioRxiv - Immunology 2020Quote: ... antibodies in RPMI (Merck), containing 10% Heat Inactivated FCS (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies (NaV1.1 Merck, AB5204 ...
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bioRxiv - Cell Biology 2023Quote: ... Detection by enhanced chemiluminescence was performed using the Immobilon Forte HRP Western substrate (ref WBLUF0500, Merck Millipore, Darmstadt, Germany) or SuperSignal West Femto Maximum Sensitivity Substrate (ref 34096 ...
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bioRxiv - Neuroscience 2024Quote: ... Membrane was washed 3x5 minutes with 50mls PBS and HRP activity was detected using ECL according to the manufacturer’s instructions (Merck Life Science UK Limited Cat no GERPN3243) ...
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bioRxiv - Cell Biology 2023Quote: ... The immunoreactive bands were detected by using enhanced chemiluminescence (ECL) reagent (Immobilon Crescendo Western HRP substrate; Merck Millipore, DE). Band densities were quantified by densitometry using Image LabTM Version 6.0.1 2017 (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2023Quote: ... Detection by enhanced chemiluminescence was performed using the Immobilon Forte HRP Western substrate (ref WBLUF0500, Merck Millipore, Darmstadt, Germany) or SuperSignal West Femto Maximum Sensitivity Substrate (ref 34096 ...
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bioRxiv - Neuroscience 2023Quote: ... after which the blot was developed with the ECL chemiluminescent reagent (WBKLS0500, Immobilon Western Chemiluminescent HRP Substrate, MERCK, USA). We obtained an intense band at ∼40kD and a very faint one at ∼32kD (Supplementary Figure 1(c) ...
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bioRxiv - Molecular Biology 2021Quote: ... and resuspended in 60 μl PFGE wash buffer containing 1 μl lyticase (17 U/μl 10 mM KPO4 pH 7, 50% glycerol Merck L2524 >2000 U/mg) then incubated for 10 min at 50°C ...
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bioRxiv - Physiology 2020Quote: ... Cows treated with the 7-day CO-Synch + CIDR protocol (n = 769) received administration of 100 μg gonadorelin acetate (GnRH; Fertagyl, Merck Animal Health, Madison, NJ) and insertion of a 1.38 g intravaginal progesterone releasing insert (CIDR® Zoetis ...
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bioRxiv - Microbiology 2020Quote: ... 48 and 72 hours) of exposure to simulated solar radiation was performed by using 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) (Sigma Aldrich-Merck KGaA, Darmstadtm Germany) solubilized in ethanol ...
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bioRxiv - Cell Biology 2023Quote: ... to validate the gene expression the plasmid (vide-supra) was transfected into HEK293 cells using PEI (Sigma-Aldrich/Merck Cat. No. 49553-93-7) following an optimized version of the standard protocol(Rajendra ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then transferred to a PVDF membrane (Merck), which was blocked with 5% dry milk ...
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bioRxiv - Genomics 2020Quote: ... Proteins were transferred to a nitrocellulose membrane (Merck Millipore) for 1h at 20V ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were electroblotted onto PVDF membranes (IPVH00010, Merck Millipore) via a semi- dry cassette (1703940 ...
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bioRxiv - Neuroscience 2020Quote: ... 1:50 Hyaluronan binding protein HABP (#H0161; Merck, Germany) or 1:100 mouse anti-Neurocan (#N0913 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred onto PVDF membranes (0.45µm, Merck Millipore) at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Separated proteins were transferred onto Immobilon-P membrane (Merck) using Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell (Bio-Rad Laboratories) ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by protein precipitation with dichloromethane (Merck, Darmstadt, Germany) and methanol (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... and the protein loading control tubulin (Merck, Sigma-Aldrich). Immuno-reactive complexes were detected using HRP-conjugated secondary antibodies (Cell Signaling Technology ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were electroblotted onto Immobilon-P transfer membranes (Merck). The membranes were blocked with Blocking One for 30 min at room temperature and then incubated with primary antibodies overnight at 4 °C ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were transferred to a PVDF membrane (Merck Millipore) after which the membrane was blocked using Odyssey Blocking buffer (LI-COR ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced with 10 mM dithioerythritol (Merck-Millipore) for 1 h at 56°C followed by a washing-drying step as described above ...
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bioRxiv - Molecular Biology 2022Quote: ... and proteins concentrated in Amicon Ultra-15 concentrators (Merck) before flash freezing and storage at - 80 °C.
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bioRxiv - Molecular Biology 2022Quote: Millipore Compartment Protein Extraction Kit (Merck-Millipore, Cat. #2145) was used to deplete cytosolic ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was concentrated using Amicon filters (Merck, Darmstadt, Germany) in buffer (50 mM HEPES pH 7.5 ...