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Citations for Merck :
551 - 600 of 2598 citations for Centrosomal Protein 63 kDa CEP63 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... pooled according to purity and afterwards concentrated using Amicon Ultra-4 centrifuge filters (30 kDa cutoff, Merck Millipore). The purified octamers were validated by SDS-PAGE ...
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bioRxiv - Genetics 2023Quote: ... Supernatant was then transferred to an Amicon Ultra-0.5 Centrifugal Filter Unit 3 kDa (Merck Millipore, no. UFC500396) and centrifuged for 45 min at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Peak fractions were pooled and concentrated using a 50 kDa molecular weight cut-off Amicon ultracentrifugal concentrator (Merck).
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bioRxiv - Molecular Biology 2023Quote: ... Eluted fractions were pooled and concentrated in a 50 kDa molecular weight cut-off Amicon ultracentrifugal concentrator (Merck) at 2,000 xg and exchanged into the same buffer used for elution (without imidazole ...
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bioRxiv - Molecular Biology 2023Quote: ... NDs were concentrated to 500-1000 µM using Centriprep concentrating units (10 kDa MWCO, Merck Millipore, Darmstadt, Germany). Lipids and DPC were purchased from Avanti Polar Lipids (Alabaster ...
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bioRxiv - Biochemistry 2023Quote: ... at 4 °C for 30 s and concentrated in a 100 kDa Amicon Ultra centrifugal filter (Merck Millipore), which was equilibrated with 1% GDN in IP buffer ...
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bioRxiv - Bioengineering 2023Quote: ... Fifteen mL of the combined medium was concentrated using Amicon Filter Units (MWCO=100 kDa, ACS510024, Merck Millipore) centrifuged at 4000g for 15 minutes ...
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Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheepbioRxiv - Bioengineering 2022Quote: ... Pooled fractions were buffer exchanged into 1X PBS using 10 kDa MWCO centrifugal spin concentrators (Merck, Cat#UFC801024), for 15 min at 4000 g ...
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bioRxiv - Biophysics 2022Quote: ... The eluent was concentrated using an Amicon Ultra-15 100-kDa cut-off centrifugal filter unit (Merck Millipore) before purification on a Superose 6 Increase 10/300 GL gel filtration column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... The collected flowthrough was pooled and concentrated using 30 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated sample was loaded onto a HiLoad Superdex S75 (16/600 ...
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bioRxiv - Molecular Biology 2022Quote: ... concentrated with an Amicon Ultra-4 centrifugal filter with a 5 kDa molecular weight cutoff (UFC8003, Merck Millipore), snap frozen and stored at -80 °C either directly (used for cryo-EM ...
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bioRxiv - Neuroscience 2023Quote: ... The solution was then subjected to six consecutive filtration cycles through 100 kDa centrifuge filters (Merck, Darmstadt, Germany) to remove excess compounds and unreacted monomers.
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bioRxiv - Microbiology 2023Quote: ... The fixed samples were washed with PBS using an Amicon Ultra centrifugal unit (MWCO: 100 kDa, Merck Millipore) and subjected to size exclusion chromatography to further purify the viral fraction as described elsewhere59 ...
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bioRxiv - Biophysics 2023Quote: ... eluted pre-ribosomes were concentrated on Amicon Ultra 0.5ml spin columns with a 100 kDa cutoff (Merck Millipore). Purification of post-catalysis Dbp10 which required depletion of SPB4 was performed by adding 3-Indole Acetic acid (IAA ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was applied to 3 kDa MWCO filters (Amicon Ultra-0.5 Centrifugal Filter; Merck Millipore, Darmstadt, Germany), and centrifuged at 14,000 × g for 45 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Samples were then loaded on Amicon Ultra-15 centrifugal filters (10 kDa molecular weight cutoff, Merck Millipore # UFC9010) for imidazole removal and washed with purification buffer ...
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bioRxiv - Microbiology 2024Quote: ... the sample was filtered in an Amicon Ultra-0.5 Centrifugal Filter Unit 10 kDa (Merck Millipore cat # UFC501096) supplemented with a 100 µl of 0.1 M Na phosphate buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were detected by incubation with primary antibodies (SOX2, Bio-techne, AF2018, 1: 1000 and α-Tubulin, Merck, T6199, 1:2000) and secondary antibodies (Donkey anti-Goat IRDye® 800CW ...
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bioRxiv - Microbiology 2023Quote: ... Cells were permeabilized with 100% ice-cold methanol for 20 min at -20°C and rehydrated by washing with PBS and then stained for viral hexon protein expression with an anti-hexon primary antibody (mouse; Merck; #MAB8052) and an anti-mouse secondary antibody coupled to AF488 or AF647 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... of cell lysates by incubating lysates with 0.5-2.5 µg of antibodies and 20 µl of protein G–Sepharose (Merck, GE17-0618-01) at 4°C for 2 h ...
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bioRxiv - Biochemistry 2019Quote: ... Cas6 and Cas6 H47N were concentrated as required using an Amicon Ultra centrifugal filter (MWCO 10 kDa, Merck-Millipore).
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bioRxiv - Biochemistry 2019Quote: ... concentrated and buffer exchanged (kinase NMR buffer, see below) by ultrafiltration (Amicon Ultra-0.5 ml, 3 kDa MWCO, Merck) for NMR analysis.
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bioRxiv - Biophysics 2019Quote: ... Further concentration and buffer exchange to 20 mM Tris-HCl pH 8.0 were carried out with Amicon Ultra-4 centrifugal filters (10 kDa cut-off, Merck). The mixture of tetramers was then purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 and 6 were pooled and concentrated to 1 ml using 10 kDa centrifugal filter (Amicon Ultra-2ml, Merck Millipore ...
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bioRxiv - Microbiology 2021Quote: ... Filtered samples were centrifuged at 5000G using an Amicon ® Ultra 15 (100 kDa) (Merck Millipore, Tullagreen, Cork, IRL) following the manufacturer’s protocol (30 min of centrifugation for every 12 ml) ...
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bioRxiv - Biochemistry 2020Quote: ... Selected FdR9-containing fractions were concentrated by ultrafiltration using a 30 kDa cut-off membrane (Amicon ultra-15, Merck) and further purified by gel filtration on a Superdex 75 26/60 column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... fractions containing the complex DrmAB were pooled together and concentrated using 30 KDa NMWL Ultra-15 Amicon® (Merck). Protein concentration was estimated by the Bradford Assay (Thermo Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... all solutions were purified using Amicon centrifugal ultra-filtration devices (Amicon Ultra-2, Sigma Aldrich, 100 kDa membrane, Merck). Prior to use ...
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bioRxiv - Biochemistry 2021Quote: ... vesiculosus fucoidan were separated by passing an aqueous fucoidan solution over a 10 kDa cut-off ultrafiltration membrane (Merck) followed by lyophilization.
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bioRxiv - Biophysics 2021Quote: ... the culture supernatant was collected and concentrated using the Amicon Ultra centrifugal filter unit with 100 kDa membrane (Merck), aliquoted and stored in -80°C.
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bioRxiv - Biophysics 2021Quote: ... The culture supernatant was collected after 72 h and concentrated using Amicon Ultra-15 MWCO 100 kDa (Merck Millipore).
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bioRxiv - Molecular Biology 2019Quote: ... from an 18500g centrifugation (6 min at +4°C) were run through 3-kDa cut-off centrifugal filters (Amicon Ultra-0.5 ml, Merck) into pre-weighted collection tubes to remove remaining macromolecules ...
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bioRxiv - Cancer Biology 2020Quote: ... CM (and the same volume of SM as control) was then concentrated by 3 kDa Amicon filters (Merck-Millipore) to a final volume of 100 μl/mouse and stored at −20°C until usage ...
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bioRxiv - Microbiology 2021Quote: ... was eluted with solution-I and was concentrated to with an Amicon Ultra 10-kDa MWCO concentrator (Merck Millipore). The protein was further subjected to size exclusion chromatography on a HiLoad 16/600 Superdex 75 column pg column (Cytiva ...
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bioRxiv - Cell Biology 2021Quote: ... 10 mL of the supernatant were concentrated using an Amicon Ultra-15 100 kDa filter (regenerated cellulose; Merck/Millipore) by centrifuging at 4.000 × g for 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Selected fractions were concentrated to 30 µl using 10 kDa cutoff centrifugal filters (Merck, Amicon Ultra 0.5ML 10K, #UFC5010). The samples were supplemented with 4x LDS (NuPAGE ...
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bioRxiv - Biophysics 2022Quote: ... Unconjugated streptavidin and Dpeg4-biotin were removed via ultrafiltration (Amicon Ultra-0.5, 100 kDa; Merck Millipore, Billerica, MA, USA). The detection media contained the prepared CF660R and Cy3-labelled detection antibodies for each antigen with final concentrations as follows ...
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bioRxiv - Cell Biology 2022Quote: ... Relevant peak fractions were pooled and concentrated with Amicon Ultra Centrifugal Filter Units 30 kDa MWCO (Merck Millipore, UFC903024) to the required concentration.
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bioRxiv - Biochemistry 2022Quote: The culture supernatant containing proMbxA was concentrated with Amicon Ultra-15 Centrifugal Filter Units (50 kDa NMWL, Merck Millipore) to 1/10 of the starting volume and loaded on a 5 ml Ni2+ HiTrap IMAC HP column (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... The mixture was concentrated using an Amicon Ultra 0.5 ml filter unit (50 kDa molecular weight cut off, Merck). A final concentration of the complex was approximately 11.2 µM ...
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bioRxiv - Cancer Biology 2021Quote: ... and then concentrated using 15 ml Amicon spin filter cartridges (cutoff: 3 kDa, 10705884-Merck Millipore, Burlington, MA, USA) by serial addition of 10 ml of CM and centrifugation at 4000 x g until 1.5 ml were left ...
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bioRxiv - Biophysics 2022Quote: ... the solution concentrated by ultrafiltration using Amicon Ultra centrifugal filters with a 10 kDa molecular weight cutoff (MWCO) (Merck) and passed over a Superdex 75 16/60 gel filtration column (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: RCA phage lysates were filtered through 0.1μm pore-size filters and then concentrated using Amicon Ultra Centrifugal Filters (30-kDa, Merck Millipore). Concentrated phages were absorbed onto copper grids in the dark and stained with 2% uranyl acetate for two minutes ...
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bioRxiv - Cell Biology 2019Quote: ... The conditioned medium was collected and concentrated using 30 kDa cutoff ultra-centrifugal filter units (Amicon, Merck-Millipore, MA). Protein concentration was determined by Bradford method [45] ...
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bioRxiv - Biochemistry 2019Quote: ... using binding buffer and concentrated using a 3 kDa high molecular weight cut-off Amicon Ultra Centrifugal Filter (Merck). SDS-PAGE was used to assess the purity of untagged SbtB7001 ...
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bioRxiv - Synthetic Biology 2021Quote: ... VLP fractions were pooled and concentrated using 100 kDa MW cut-off centrifugal filters (Amicon Ultra 4 ml, Merck). Protein concentrations were measured using the linearised Bradford method54 with Pierce Coomassie Protein Assay Kit (Thermo Scientific).
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bioRxiv - Biophysics 2020Quote: ... beads were removed and recombinant dynein was concentrated in a 100 KDa cut-off filter (Amicon Ultracel, Merck-Millipore) to a final volume of 500 μL ...
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bioRxiv - Cell Biology 2020Quote: ... Eluted GFP fusion proteins were concentrated and buffer exchanged into buffer PB (500 mM NaCl, 50 mM Tris-HCl, pH 8.0) using 30 kDa Amicon centrifugal filters (Merck). Eluted bdSENP1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions were analysed by SDS-PAGE and the homogenous peak fractions were pooled and concentrated to 4.5 mg/ml using a 100 kDa MWCO Amicon Ultra Centrifugal Filters (Merck). Concentrated Mediator was aliquoted ...
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bioRxiv - Molecular Biology 2022Quote: All samples were centrifuged at 16,000 × g for 30 min and 400 µL supernatant was loaded on a 0.5 mL centrifugal ultrafiltration unit with 3 kDa cut-off membrane (Amicon, Merck) and concentrated down to 20 µL ...