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Citations for Merck :
551 - 600 of 4916 citations for 7 bromo 1 methyl 1 3 dihydro 2H benzo d imidazol 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The lipids were resuspended in a small volume of chloroform/methanol (2:1) and applied to a to HPTLC plate (Silica Gel 60, Merck, Germany) together with DOPC/cholesterol and NeuGc/NeuAc GM3 as standards ...
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bioRxiv - Microbiology 2020Quote: ... and the other stored at −80 °C in a sterile 2 ml cryovial containing 1 ml of a 30 % glycerol solution, prepared by diluting glycerol (≥99 %, G2025, Sigma-Aldrich (Merck), Overijse ...
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bioRxiv - Biochemistry 2022Quote: ... The eluate of the affinity purification was concentrated to a volume between 1-2 mL before injection using centrifugal filter units (Amicon, Merck millipore) with a MWCO of 5,000 Da ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... were maintained in Opti-MEM™ I Reduced Serum Medium + GlutaMax™ (Thermo Fischer Scientific) supplemented with 2% foetal bovine serum (FBS, Thermo Fischer Scientific) and 1% Anti-Anti 100X (Merck) at 37 °C in a humified atmosphere of 5% CO2 ...
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bioRxiv - Plant Biology 2023Quote: ... and concentrated to 2 mg chlorophyll mL-1 using a 100-kDa molecular-weight cut-off centrifugal filter unit (Amicon Ultra-15, Merck Millipore). A 3 µl volume of the sample was applied to a glow-discharged holey carbon grid (GIG ...
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bioRxiv - Cell Biology 2023Quote: ... Transfectants with stably integrated GOI were then selected based on their resistance to G418 (0.5 mg/ml, 1-2 weeks, Merck, Cat.N. G8168). To induce the expression of the GOI the cells were treated with doxycycline (2 μg/ml ...
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bioRxiv - Synthetic Biology 2023Quote: ... The routinely used growth medium was composed of yeast extract (1%(w/v)) (Fisher BioReagents™) and casein peptone (2%(w/v)) (Merck), and was supplemented with 2% (w/v ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 1-2 µg of pcDNA5 FRT/TO plasmids containing the gene of interest using GeneJuice transfection reagent (Cat#70967, Merck Millipore) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: Plants were grown on 1/2 MS agar plates containing 0.01% (v/v) dimethyl sulfoxide (mock) or 50 ng mL-1 TM (654380; Merck, Darmstadt, Germany) for 14 days ...
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bioRxiv - Paleontology 2023Quote: All aqueous solutions were prepared from ultrapure grade water obtained by water filtration with a two stages Millipore system (Milli-Q® Academic with a cartouches Q-Gard 1 and Progard 2, Merck Millipore ...
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bioRxiv - Microbiology 2024Quote: ... ParT (S48C)-His6 and ParT (Q271C)-His6 aliquots were supplemented with 1 mM tris (2-carboxyethyl) phosphine hydrochloride (Merck; cat# C4706) before flash-freezing in liquid nitrogen.
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bioRxiv - Cancer Biology 2024Quote: ... 2 million cells per mL were suspended in 1 mL of a 1.2% sodium alginate solution (Merck, Saint-Quentin-Fallavier, France). Beads were formed by dripping the cell suspension into a sterile 100 mM CaCl₂ solution (VWR ...
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bioRxiv - Developmental Biology 2021Quote: We mated Exosc10+/+ or Exosc10+/− females with stud Exosc10+/− male mice and recovered two-cell stage (gestational day 2; GD2) embryos by flushing approximately 0.5 ml of EmbryoMax Advanced KSOM medium (Merck-Sigma, MR-101-D) into the oviduct ...
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bioRxiv - Neuroscience 2023Quote: ... We replenished the medium the next day and started selection against replicating non-neuronal cells on day two using neuronal medium containing 2 μM Cytosine β-D- arabinofuranoside (AraC) (Merck Life Sciences). We gradually diluted AraC out by replacing half of the medium with fresh neuronal medium every other day ...
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bioRxiv - Cell Biology 2020Quote: ... permeabilized in 1% DMSO/1% Triton X-100 and stained with anti-phospho H3 antibody (1:250, Merck Millipore) followed by anti-rabbit Alexa 488 secondary antibody (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... 2 µL lysis buffer (15 mM NaHEPES pH 7.3, 30 mM NaCl, 1 mM MgCl2, 1% Brij35, 1 U/µL benzonase (Merck), 1X complete protease inhibitor (EDTA-free ...
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bioRxiv - Biochemistry 2023Quote: ... was incubated in 5 mM Tris-HCl buffer (pH 7.5) containing each substrate (1% glucomannan, Neogen, MI, USA; 1% polygalacturonic acid, Neogen; 1% carboxymethyl cellulose, Merck; 1% soluble starch ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated with medium supplemented with 20 μg/ml actinomycin D (Act D; Merck) for 1 h to inhibit cellular transcription ...
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bioRxiv - Neuroscience 2022Quote: ... and one 11 µM nylon filter (NY1102500, Merck Millipore). The filtrate was then centrifuged at 1500 RCF for 15 minutes at 4 °C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... with one round of Tri-Reagent® (Merck Millipore). cDNA synthesis was conducted according to manufacturer’s protocol with 500 ng of RNA ...
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bioRxiv - Microbiology 2023Quote: ... slick SSW and non-slick SSW from sites #1 – #3 (Fig. 1b) were fixed with 25 % glutardialdehyde (0.5 % final concentration, Sigma-Aldrich/Merck Life Science AB, Solna, Sweden) and stored at −80 °C ...
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bioRxiv - Immunology 2024Quote: ... we included αTIM-3+ αPD-1 and used anti-human RSV-IgG4 as an isotype control antibody (5 μg/mL, 60AGK S228P, Merck & Co., Inc., Rahway, NJ, USA). On day six ...
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bioRxiv - Molecular Biology 2021Quote: ... 3% casein (Merck) in 20 mM TBS (pH 11.0) ...
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bioRxiv - Cancer Biology 2023Quote: ... Nutlin-3 (Merck) was dissolved in DMSO and diluted in saline.
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P2RX7 inhibition reduces breast cancer induced osteolytic lesions - implications for bone metastasisbioRxiv - Cancer Biology 2022Quote: ... The cells were then stimulated with 100μM 2’(3’)-O-(4-Benzoylbenzoyl) adenosine-5’-triphosphate (BzATP; Merck Life Sciences, Gillingham, UK) to activate the P2RX7 ...
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bioRxiv - Microbiology 2023Quote: MDCK cells (2 x 105 cells) were cultured on polycarbonate Millicell culture plate inserts (12- mm diameter, 3-µm pore size; Merck Millipore) at 37°C under 5% CO2 atmosphere.
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bioRxiv - Microbiology 2021Quote: ... The suspension was treated with DNase I (1 μg ml-1) and RNase A (100 μg ml-1, Merck, India) to degrade nucleic acid residues of the host bacteria ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR amplification of the Round 1 PCR product (1 ul) with 1 ul of JH reverse primer (10 uM, provided by MERCK) and 1 ul of FR1 forward primer set pools (10 uM per primer ...
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bioRxiv - Biochemistry 2023Quote: ... resuspended in 8 mL lysis buffer (5 mM imidazole in 100 mM potassium phosphate buffer pH 8.0) supplemented with 1.25 g mL−1 of lyophilized lysozyme and 1 U mL−1 of benzonase nuclease (Merck Millipore), and lysed with an EmulsiFlex-B15 cell disruptor (Avestin) ...
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bioRxiv - Cell Biology 2020Quote: ... 1% Penstrep and 1 ng/ml TGFβ (Cat nb. T7039, Merck, Sigma) or 20 ng/ml PDGF-AA (human PDGF-AA ...
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bioRxiv - Biophysics 2022Quote: ... cells were diluted in in HEPES buffered DMEM/F12 1:1 (Merck) supplemented with 10% fetal bovine serum and 2 mM L-glutamine to 50,000 cells/ml and plated at a density of 5000 cells per well (100 µl ...
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bioRxiv - Plant Biology 2020Quote: ... supplemented with 1 mM PMSF and 1 X cOmplete protease inhibitors (Merck) and lysed with a French press under 1200 PSI ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... and goat anti-Mouse IgG-HRP (1:1000-1:3000, Merck Millipore). The kinase reactions using recombinant McIdas were performed as follows ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... and goat anti-Mouse IgG-HRP (1:1000-1:3000, Merck Millipore).
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg/mL (1:500 dilution) of biotinylated anti-phosphotyrosine 4G10 (Merck) antibody and incubated for 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... rabbit anti-PRRX16 (1:200) rabbit anti-Laminin (Merck, #L9393 1:200). Secondary antibodies used in these studies were procured from Thermo Fisher Scientific (Waltham).
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bioRxiv - Biochemistry 2024Quote: ... 1:1’000) and mouse anti-EF1a (Merck Millipore, dilution WB 1:10’000). Antibodies previously produced in our laboratory are ...
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bioRxiv - Molecular Biology 2023Quote: ... All samples were fixed 1:1 with 4% PFA (Merck, no. P6148) (final concentration 2% PFA ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 mM MgCl2 1 mM DTT and 1× cOmplete protease inhibitor (Merck)] were added and incubated on the rotating wheel for 2 h at 4ºC ...
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bioRxiv - Biochemistry 2023Quote: ... A 1:1 mixture of magnetic hydrophobic and hydrophilic Seramag speedbeads (MERCK) were added at a 2:1 (w/w ...
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bioRxiv - Genetics 2024Quote: Cells were lysed in 1% SDS plus 1:200 Benzonase (Merck, #E1014) for 20 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... and 1 mL of 65 mg mL−1 sulfo-NHS (Merck 56485) were added to the reaction ...
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bioRxiv - Synthetic Biology 2024Quote: ... 6.16 µM ZnCl2 (Merck/Sigma-Aldrich, CAS number : 7646-85-7), 0.76 µM CuCl2·2H2O (Merck/Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.04 mM cytosine (Merck/Sigma-Aldrich, CAS number : 71-30-7), 0.04 mM uracil (Merck/Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... After cooling the samples to room temperature 7 units Benzonase (Merck) were added to each sample and incubated for 30 min at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... the reduced cysteine residues were blocked employing a 10 mM solution of S-methyl methanethiosulfonate (MMTS) (Merck KGaA, Darmstadt, Germany) at room temperature for 10 minutes ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Adult mosquitoes were provided ad libitum access to 10percent sugar solution (8percent sucrose +2percent glucose +0.05percent methyl paraben +5percent vitamin syrup (Polybion SF Complete, Merck Ltd.)) ...
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bioRxiv - Biochemistry 2021Quote: ... After adding 1 drop of bovine thrombin (Biomed, Lublin, Poland) to 2 drops of fibrinogen (1mg/ml; Sigma-Aldrich, St. Louis, MO; Merck KGaA) the cells were entrapped within the fibrin clots ...
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bioRxiv - Cell Biology 2021Quote: ... ethanol and cut into 1–2 mm3 pieces and was cultured in Dulbecco’s modified Eagle medium (DMEM) (Sigma-Aldrich, Merck, Darmstadt, Germany) with 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Media was concentrated further to a final volume of 1-2 ml using 10 KDa Amicon spin filters (Merck Millipore, MA, USA) by centrifugation at 3,500 g ...