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Citations for Merck :
551 - 600 of 3062 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... slices were incubated for 24-48h at 4°C with mouse anti-Neurophysin I (1:2000, Merck Millipore, marker for Oxytocin), rabbit anti-vasopressin (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Separated proteins were wet-blotted (110 V; 90 min; 4°C) on fluorescence-optimized polyvinylidene difluoride (PVDF) membranes (Immobilon-FL; Merck; IPFL00010) in transfer buffer (25 mM Tris (pH 7.6) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatants were transferred to new tubes and incubated overnight at 4 °C with 15 µl of Anti-FLAG® M2 Magnetic Beads (Merck) with gentle mixing ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were first concentrated by centrifugation at 4,000 x g at 21°C using Amicon® Ultra-4 Centrifugal Filter Unit with 100kDa molecular weight cutoff (UFC810024, Merck Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated overnight at 4°C with primary antibodies against AVP neurophysin II (NP-II; MERCK, MABN845, PS41; 1:200); OXT NP-I (PS38 ...
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bioRxiv - Molecular Biology 2024Quote: ... Remaining chromatin extracts were incubated overnight at 4°C with 50 µL of prewashed anti-Flag M2 magnetic beads suspension (Millipore, Merck M8823) per sample ...
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bioRxiv - Plant Biology 2024Quote: ... 6xHis:SidK:3xFLAG or 6xHis:SidKF62A:3xFLAG protein were added to 1 ml of filtered extract to a final concentration of ∼2.5 μM and incubated in a rotatory mixer at 4°C with 25 μl of Anti-FLAG® M2 Magnetic Beads (Merck). 30 μl of the mixture was taken as input for Western blot analysis before adding magnetic beads ...
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bioRxiv - Neuroscience 2024Quote: ... followed by overnight incubation at 4°C with primary antibodies (synaptophysin Cat#MAB329, Merck Millipore; GluA2 Cat#13607, Cell Signaling; Psd95 Cat#MAB1598, Merck Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were blocked in 10% normal goat serum in TBS-T for one hour at room temperature and then incubated in the following primary antibodies suspended in blocking buffer overnight at 4°C: pS129 (El-Agnaf laboratory, 1µg/ml 27) and 5G4 (MABN389, Merck, 2µg/ml) combined with either MGO-aSyn (1µg/ml ...
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bioRxiv - Immunology 2021Quote: ... Cloning of the antigen library into the T7 phages was performed following manufacturer’s instructions (T7Select 10-3 cloning kit, catalog no. 70550-3, Merck-Millipore, Burlington, MA, USA).
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bioRxiv - Cell Biology 2024Quote: ... The coverslips were rinsed with 100% methanol before being functionalised in an amino-silane solution (3% vol/vol (3-aminopropyl) triethoxysilane (Merck cat no. 440140), 5% vol/vol acetic acid (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... 5×106 cells from treated and untreated conditions were centrifuged for 5 min at 4,500 g and the pellets were resuspended in 4% paraformaldehyde (PFA, Merck, Germany), and incubated for 20 min ...
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bioRxiv - Molecular Biology 2023Quote: ... equipped with a LiChrospher® 100 RP-18 (125 mm x 4 mm, 5 µm) C18 reversed-phase column (Merck). The elution solvents consisted of ultrapure water with 0.1% formic acid (A ...
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bioRxiv - Physiology 2024Quote: ... The tissues were fixed in 4% paraformaldehyde and were blocked with 5% normal donkey serum (NDS) (Merck, Cat# D9663, RRID:AB_2810235), 0.2% tween-20 in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2021Quote: ... protease cleavage site at the N-terminus site was subcloned between KpnI (5’-terminus) and HindIII (3’-terminus) restriction enzyme sites in the pRSF-1b vector (Merck, Darmstadt, Germany) to express His-TEV protease site-MA protein (pRSF-1b_MA ...
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bioRxiv - Molecular Biology 2024Quote: ... fixed with 2% paraformaldehyde (PFA) in PHEM and washed once for 5 min at (RT) with 3% BSA (bovine serum albumin, Merck-Sigma, Germany) in Tris-buffered saline with 10 mM EGTA and 2 mM MgCl2 (TBSTEM) ...
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bioRxiv - Biochemistry 2023Quote: ... dehydrated with 100 μL acetonitrile and rehydrated with 25 μL trypsin solution, containing 5 ng/μL trypsin (cat # 37283, SERVA Electrophoresis GmbH, Heidelberg) in 3 mmol/L ammoniumbicarbonate (cat # 09830, Merck KGaA, Darmstadt). After 4 h incubation at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... 10-15 neurospheres and 4-6 organoids per cell line were rinsed twice with PBS and then lysed in RIPA buffer (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) supplemented with Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... They were recorded in the presence of 10 mM CNQX (6-cyano-7-nitroquinoxaline-2,3-dione, Merck) and 50 mM D-AP5 (D-2-amino-5-phosphonovalerate ...
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bioRxiv - Cell Biology 2022Quote: Metaphase II-arrested eggs (at least 4 hours after polar body extrusion) were treated for 4 hours with: Cytochalasin D (C8273-1MG, Merck) at a final concentration of 5 μg/ml in M2 medium or Latrunculin B (428020-1MG ...
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bioRxiv - Cell Biology 2024Quote: ... Experiments for Fig.4 and Extended Data Fig.4 were done using CellASIC ONIX V1 microfluidics platform using Y04C microfluidics plates (Merck), at 32° in YE4S ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...
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bioRxiv - Microbiology 2020Quote: ... Expanded and chopped gels were stored at 4°C in ddH2O immobilized prior to imaging on PDL-coated glass chambers (Merck, 734-2055).
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2022Quote: ... for 5 min each at RT and incubated with secondary antibodies in TPBS-TS-1% for 4h at 4°C (Alexa Fluor 546 anti-Mouse (Merck, A-11018), Alexa Fluor 488 anti-goat (Abcam ...
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bioRxiv - Pathology 2022Quote: ... The sections were incubated overnight at 4°C in a pre-incubation solution containing mouse monoclonal em48 (Merck Millipore; MAB5374, 1:400) and rabbit polyclonal Laminin (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... 0.1% (20mM Tris and 500mM NaCl pH 7.5) and incubated overnight at 4°C with the primary antibody: anti-GluN1 (1:1000; Merck KGaA, Darmstadt, Germany) and anti-GluN2A monoclonal antibody (1:1000 ...
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged (16,000 g, 10 min, 4°C) and supernatants measured for protein content using infrared spectroscopy (Merck Millipore, Darmstadt, Germany). Lysates were then reduced ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were permeabilized for 10 minutes in 0.5% Triton X in PBS and incubated for 48 hours at 4°C in rabbit anti-NG2 (AB5320, Merck Millipore; 1:500) and rat anti-MBP (MCA409 ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted 1:2000 and incubated overnight at 4°C followed by an secondary anti-rabbit HRP conjugated antibody (12-348, Merck Millipore, Germany) diluted 1:5000 in PBST and incubated for 1 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue homogenisation and western blotting was performed with 50 μg of total protein as previously described.15 Membranes were incubated overnight with gentle agitation at 4 °C with the MSH3 antibody (Merck MSH3MAB(1F6)) in 5% milk PSB-T (phosphate buffer saline (PBS) ...
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bioRxiv - Bioengineering 2023Quote: ... at 40,000 xg for 20 min at 4°C and the supernatant was filtered through a 0.45 µm Millex® syringe filter (Merck, Darmstadt, Germany). Clarified lysate was loaded onto a nickel (II ...
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bioRxiv - Biophysics 2023Quote: ... containing 0.3□mM biotin and concentrated at +4 °C to 25 μl volume using Amicon ®Ultra 10 kDa MWCO centrifugal filters (Merck Millipore).
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bioRxiv - Microbiology 2024Quote: ... the SN was concentrated by ultrafiltration at 4000□g and 4°C using 100 K Amicon® Ultra-15 centrifugal filters (Merck Millipore). The concentrated SN was filtered through a 0.45-µm-pore-size filter ...
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bioRxiv - Cell Biology 2021Quote: ... then post-fixed in 4% PFA (Merck, 8187081000) at room temperature for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... using RP select B 125-4 Column (Merck). The chromatography was carried out at a flow rate of 1.5ml/min starting with a mobile phase of 80% methanol and 20% H2O for 3 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed with 4% PFA (Merck, #104005) in PBS at room temperature for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... and 0.1 μL Benonase (9025-65-4, Merck) was added to each 1 mL of crude virus extract to remove cell genome and plasmid DNA ...
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bioRxiv - Microbiology 2021Quote: ... followed by 4 mL of chloroform (Merck Millipore) and mixing ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were then fixed in 4% PFA (Merck) and immunostained with mouse monoclonal anti-SARM1 primary antibody (Chen et al. ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 4% PFA (Merck or Sigma-Aldrich). The brains were collected and fixed overnight in 4% PFA in PBS at 4°C ...
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bioRxiv - Immunology 2020Quote: Cryosections were fixed with 4% paraformaldehyde (PFA, Merck) and stained with primary and fluorescent-labeled secondary antibodies as described (Figel et al. ...
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bioRxiv - Genomics 2020Quote: ... filters were fixed with 4% paraformaldehyde (PFA, Merck) and permeabilized with 0.1% Triton X-100 (Merck) ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 4% paraformaldehyde (Merck, Whitehouse Station, USA) in 0.1 M PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... and tamoxifen (1uM 4-hydroxy-tamoxifen, Merck, Australia) treatments ...
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bioRxiv - Cell Biology 2020Quote: Cells were fixed with 4% PFA (1.04005.1000, MERCK), 4% sucrose and 0.1 M phosphate buffer (pH 7.2 ...
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bioRxiv - Cell Biology 2022Quote: ... Treatments were performed with hydroxyurea (4 mM, Merck), the MRE11 inhibitor mirin (25 µM ...
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bioRxiv - Neuroscience 2024Quote: ... 4 µM SU5402 (both from Merck Life Sciences) and 1 µg/ml doxycycline for 3 days ...
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bioRxiv - Molecular Biology 2024Quote: ... intracellular parasites were fixed in 4% formaldehyde (Merck) and 0.0075% glutaraldehyde in 1X PBS for 30 minutes ...