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Citations for Merck :
5701 - 5750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... in 30% (2-Hydroxypropyl)-β-cyclodextrin (HPβCD) or Sulfobutylether-β-Cyclodextrin (SBEβCD) (Sigma Aldrich, Merck, Germany). When tumors reached a size of 60-100 mm3 ...
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bioRxiv - Biochemistry 2023Quote: Human osteosarcoma SJSA-1 cells were purchased from ATCC (Manassas, VA, USA) and cultured with RPMI 1640 medium (Sigma Aldrich, Merck, Germany), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2023Quote: ... was used to plot elution profiles of the protein standard mix Supelco® 69385 Protein Standard Mix 15-600 kDa (Sigma-Aldrich/Merck, Darmstadt, Germany), and the analyzed protein samples.
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bioRxiv - Biochemistry 2023Quote: ... and 1 × protease inhibitor mixture (Merck)) and centrifuged at 20,000 x g for 10 min at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... 1 × phosphatase inhibitor mixture (Merck) and 1 × protease inhibitor mixture (Merck) ...
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bioRxiv - Biochemistry 2023Quote: All peptides used in this study probes were designed as 11-mers bearing biotinylated PEG2 linker in the N terminus and an amide group in the C terminus and were synthesized using Fmoc-solid-phase peptide chemistry with Novasyn TGR resin (Merck Millipore) as described in the Novabiochem Innovations 01/11 ...
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bioRxiv - Biochemistry 2023Quote: Hippocampal cells (5.104 cells/well) were treated with 10 and 30 μmol L-1 H2O2 solutions [from H2O2 stock solution 30% (Merck, Germany), into supplemented neurobasal medium] fresh made ...
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bioRxiv - Biochemistry 2023Quote: Mouse neuroblastoma N2a-APPswe cells were cultured in Millicell EZ SLIDE 8-well glass slides (Merck). After treatments cells were washed with PBS (3 times ...
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bioRxiv - Biochemistry 2023Quote: ... concentration and buffer exchange to TE (10 mM Tris-HCl pH7.5 and 1 mM EDTA) with an Amicon Ultra-4 centrifuge filter unit42 (30 kDa cut-off, Merck). Concentration of all plasmids was measured by NanoDrop One (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: Initial substrate screens on pNP-α-Araf (Merck), pNP-β-Xyl (Merck) ...
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bioRxiv - Biochemistry 2023Quote: ... the reaction buffer was exchanged to the lysis buffer five times by Amicon Ultra 10K (Merck Millipore). Then ...
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Docking Domain Engineering in a Modular Polyketide Synthase and its Impact on Structure and FunctionbioRxiv - Biochemistry 2023Quote: ... Proteins were concentrated with Amicon Ultra centrifugal filters from Merck Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... Clarified lysates were normalised across samples and were added to 100 μl anti-FLAG M2 affinity gel (Merck) Micro Bio-Spin columns (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... mouse monoclonal FLAG-HRP (Sigma-Aldrich (Merck), A8592) ...
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bioRxiv - Biochemistry 2023Quote: Total RNA extraction was performed using the TRI Reagent (Merck, product code T9424) following the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2023Quote: ... 0.2 % cholesteryl hemisuccinate (CHS) (Merck) and rotated for 1 h at 4 °C.
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bioRxiv - Biochemistry 2023Quote: Luria-Bertani (LB) agar (Merck) plates were inoculated with Saccharomyces cerevisiae ...
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bioRxiv - Biochemistry 2023Quote: ... Desalting was performed with C18 Zip Tips (Merck) as per manufacturer guidelines ...
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bioRxiv - Biochemistry 2023Quote: ... Buffer exchange to 1xHBS and protein concentration was conducted by ultrafiltration (Amicon Ultra 10 kDa MWCO, Merck).
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bioRxiv - Biochemistry 2023Quote: ... 150 mM NaCl) and concentrated by ultrafiltration (Amicon Ultra 50 kDa MWCO, Merck).
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bioRxiv - Biochemistry 2023Quote: ... sodium acetate and formic acid (FA) were obtained from Merck (Darmstadt, DE). Acetonitrile and 0.1% FA were obtained from Biosolve (Valkenswaard ...
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bioRxiv - Biochemistry 2023Quote: ... followed by an Amicon ®Ultra centrifugation filter (3 kDa cut-off, Merck). The sample was transferred to a 5 mm NMR-tube (Labscape™ Essence ...
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bioRxiv - Biochemistry 2023Quote: ... the samples were deproteinized with a 3 kDa MWCO concentrator (Merck Millipore). To measure CoA levels ...
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bioRxiv - Biochemistry 2023Quote: ... at 4 °C for 30 s and concentrated in a 100 kDa Amicon Ultra centrifugal filter (Merck Millipore), which was equilibrated with 1% GDN in IP buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting sample of holo-SmLPMO10A was concentrated to ~ 150 μM using an Amicon ®Ultra centrifugation filter (3 kDa cut-off, Merck) and the sample was split into three equal volumes of ~ 500 μL ...
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bioRxiv - Biochemistry 2023Quote: Milli-Q water was generated from Merck Milli-Q IQ 7003 system (Darmstadt ...
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bioRxiv - Biochemistry 2023Quote: In situ protein-protein interaction was evidenced using Duolink® Proximity Ligation Assay (Merck, DUO920082, DUO92004, DUO92008). Cells were fixed with formaldehyde and permeabilized using ice-cold 90% methanol ...
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bioRxiv - Biochemistry 2023Quote: ... Then the protein was concentrated with centrifugal concentrators (UFC901096, Merck Millipore, Cork, Ireland), and loaded onto a SuperoseTM 6 Increase 10/300 GL gel-filtration column (29- 0915-96 ...
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bioRxiv - Biochemistry 2023Quote: ... The peak fractions containing the protein were pooled and concentrated in centrifugal concentrators (CLVG01230, Merck Millipore, Cork, Ireland) to a final concentration of 30 mg/ml for crystallization ...
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bioRxiv - Biochemistry 2023Quote: ... PYR-41 (Merck, 662105) or Bafilomycin A1 (Biozol ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were treated with stock solutions of MG-132 (Merck, 474790), PYR-41 (Merck ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins purified under native conditions were concentrated to 400 μL using a 10 kDa molecular weight cutoff (MWCO) Amicon Ultra-4 centrifugal spin concentrator (Merck-Millipore, Burlington, MA) and purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 column (Cytiva) ...
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bioRxiv - Biochemistry 2023Quote: ... then transferred to a polyvinylidene difluoride (PVDF) membrane (IPVH00010; Merck Millipore, Burlington, MA, USA). Antibodies against UFM1 (ab109305 ...
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bioRxiv - Biochemistry 2023Quote: ... 200 μl of IP buffer and 10 μl of anti-FLAG M2 Affinity Agarose Gel (A2220, Merck Millipore) were added to the 200 μl of lysate ...
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bioRxiv - Biophysics 2023Quote: ... After transferring the proteins onto a PVDF membrane (Merck Millipore), the membranes were blocked in TBS-T (20 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... or FGFJI (20 ng ml−1, bFGF, R&D Systems; and 200 nM JAK inhibitor, Calbiochem, Merck Millipore, Germany). Cells were collected for mechanical characterization and RNA-Seq after 5-day exposure to the respective culture conditions (30).
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bioRxiv - Biophysics 2023Quote: ... and 50% formamide (Merck, S4117) for 30 minutes in RT ...
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bioRxiv - Biophysics 2023Quote: ... and ECL films (GE28-9068-37, Merck Millipore). Films were developed in an OptiMax X-ray film processor (KODAK ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% (v/v) fetal bovine serum, 1 mM sodium pyruvate (gibco, 11360039) and 1% antibiotic/antimycotic solution (Merck, A5955). Medium was refreshed every 3 days during cultivation and every 24h in an experimental setting ...
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bioRxiv - Biochemistry 2023Quote: ... and rabbit monoclonal K48-specific Ub (Apu2) (Merck, 05-1307). The following secondary antibodies were used ...
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bioRxiv - Biochemistry 2023Quote: ... rabbit monoclonal K63-specific Ub (Apu3) (Merck, 05-1308), and rabbit monoclonal K48-specific Ub (Apu2 ...
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bioRxiv - Neuroscience 2023Quote: ... IAPP was measured by an enzyme-linked immunosorbent assay (ELISA; Merck Millipore, EZHA-52K).
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bioRxiv - Molecular Biology 2023Quote: ... for 3 h or with 100 ng/ml nocodazole (M1404, Merck) for 6 h ...
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bioRxiv - Molecular Biology 2023Quote: ... soluble contents of cells were pre-extracted before fixation by incubation for 1 min in 0.1% Triton (T8787-50ML, Merck) diluted in PBS 1X (10010-015 ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated the slides for 5 min in fresh 5% GIEMSA solution (1.09203, Merck) diluted in 100 ml Gurr buffer (10582-013 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then induced for EmGFP-NSD3 expression with 2 μg/ml of doxycycline (D9891-1G, Merck) for 48 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were treated with the Immobilon ECL Ultra Western HRP substrate (WBKLS0500, Merck), and signals were detected using an Amersham Imager 680 (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... Following saturation for 1 h in PBS 1X containing 5% milk and 0.1% Tween 20 (655204-100ML, Merck), the membranes were incubated overnight at 4 °C with primary antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... or using Amersham Hyperfilm ECL film (GE28-9068-35, Merck). Quantification of band signal intensity was done with Fiji’s analyse gels tool.
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bioRxiv - Molecular Biology 2023Quote: ... and 0.5 mM dithiothreitol) supplemented with EDTA-free protease inhibitor tablets (5892953001, Merck) and a homemade phosphatase inhibitor cocktail (final concentrations of 5 mM sodium fluoride ...