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Citations for Merck :
501 - 550 of 1367 citations for Recombinant Human APCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Protein was concentrated using an Amicon 10 kD filter column (Merck UFC801008D) and purity tested by SDS-PAGE.
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bioRxiv - Molecular Biology 2021Quote: ... proteins were transferred from the gel to a PVDF membrane (Merck, IPFL00010) by semi-dry transfer with Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-Ubiquitinylated proteins,clone FK2 (cat# 04-263, Merck Millipore; 1:1000). Ponceau solution was prepared with Ponceau BS (cat# B6008 ...
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bioRxiv - Biophysics 2020Quote: ... polyclonal antibody against brain lipid binding protein (BLBP) (Millipore, Merck KGaA, Germany), mouse antibody against nestin (Millipore ...
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bioRxiv - Plant Biology 2019Quote: ... Protein concentrations were measured using a Direct Detect® Infrared Spectrometer (Merck).
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bioRxiv - Neuroscience 2019Quote: ... Antibodies used: green fluorescent protein (GFP) mouse monoclonal (MAB3580; Merck; 1:1,000); EPAC2 monoclonal (A-7 ...
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bioRxiv - Physiology 2020Quote: ... proteins were transferred to a PVDF membrane (Immobilon-P, Merck Millipore, IPVH00010) with 160 mA for 90 min ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were visualized by ECL (Enhanced chemiluminescence; Merck Millipore, Cat. No. WBKLS0500). The signal was detected using Vilber FUSION-SL system ...
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bioRxiv - Neuroscience 2020Quote: ... the protein solution was filtered through a 100 kDa filter (MERCK, MRCFOR100). The filtrate was transferred to a low-protein binding tube and 20 equivalents of dopamine (final concentration ...
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bioRxiv - Biochemistry 2021Quote: ... All remaining protein constructs were cloned into the pET28b vector (Merck Millipore). Unless otherwise stated ...
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bioRxiv - Biophysics 2021Quote: ... Separated proteins were transferred onto a PVDF membrane (IPVH00010, Immobilon-P; Merck) and incubated for 2hr with blocking buffer containing 5% Skimmed milk (70166 ...
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bioRxiv - Plant Biology 2020Quote: ... Protein concentrations were measured using a Direct Detect® Infrared Spectrometer (Merck).
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bioRxiv - Microbiology 2020Quote: Infected insect cells were disrupted with CytoBuster™ protein extraction reagent (Merck) and clarified by centrifugation at 4,300 × g for 20m before column loading ...
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bioRxiv - Microbiology 2020Quote: ... Target proteins were visualized with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore).
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bioRxiv - Microbiology 2019Quote: ... Protein quantification was performed using the Direct Detect® system (Merck-Millipore). Each sample was set to 40 µg of total protein in 100 µl in 100 mM TEAB ...
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bioRxiv - Microbiology 2020Quote: ... GST bound protein was then cleaved using biotinylated thrombin (Merck Millipore; 69672) according to the manufacturers instructions overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred onto the methanol-activated µm PVDF membrane (Merck #ISEQ85R) using 1X transfer buffer (2.5 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: The Magna RNA-binding protein immunoprecipitation kit (Merck Millipore, Billerica, MA, USA) was applied for the assay ...
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bioRxiv - Biophysics 2020Quote: ... The protein was filtered through a 0.22 μm Millex-GV filter (Merck) to remove potential aggregates ...
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bioRxiv - Cell Biology 2021Quote: Co-IP was performed using Protein G Immunoprecipitation Kit (Merck, Cat # IP50) as per the manufacturer's protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were concentrated using 30K Amicon Ultra Centrifugal Filters (Merck, Darmstadt, Germany), flash-frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were transferred to Immobilon-P PVDF membrane (Merck Millipore, MA, U.S.A). The membranes were incubated for 1h using 5% low-fat milk solution in 1X TBS (50 mM Tris-Cl ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were electrotransferred onto an Immobilon-fl polyvinylidene difluoride (PVDF) membrane (Merck) at 30 V for 1 hour followed by100 V for a further hour at 4°C in 1× NuPAGE Transfer Buffer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Following transfer of the proteins onto a PVDF membrane (Merck Milipore, ISEQ00010), the blots were saturated with 5% BSA in Tris-buffered saline (TBS ...
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bioRxiv - Immunology 2022Quote: ... Proteins were transferred to Immobilon-FL polyvinylidene difluoride (PVDF) membranes (Merck Millipore), which were subsequently blocked with 5% blotting grade blocker (cat# 170-6404 ...
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bioRxiv - Biochemistry 2022Quote: ... Selenomethionine-labelled proteins were expressed in Escherichia coli B834(DE3) cells (Merck). All other proteins were expressed in Escherichia coli BL21(DE3 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The resolved proteins were electrotransferred to an Immobilon-P membrane (Merck, IPVH00010). The membrane was probed with specific antibodies listed in Supplementary Table 3 ...
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bioRxiv - Neuroscience 2020Quote: ... Protein lysates were concentrated using Microcon-10kDa Centrifugal Filter Units (Merck Millipore), according to manufacturer’s instructions and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... The desalted protein was concentrated on an Amicon 10,000 MWCO (Merck, Germany) and filtered through a 0.22 µM membrane.
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bioRxiv - Microbiology 2019Quote: ... Total protein was isolated from whole cell lysates using BugBuster® (Merck). Total protein concentrations were quantified by Pierce BCA quantification kit (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2019Quote: ... Fractions with addition of thrombin protease (5 Units/mg protein, Merck, www.merckmillipore.com) were pooled and dialyzed against dialysis buffer (10 mM Tris ...
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bioRxiv - Cancer Biology 2019Quote: ... and resolved protein was transferred to Immobilon-P PVDF 0.45µm Membrane (Merck). Proteins were detected using primary antibodies to Actin (Santa Cruz ...
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bioRxiv - Cell Biology 2019Quote: ... anti-pro-surfactant-protein-C (rabbit, 1:20,000, Merck, AB3786, pSP-C), anti-rabbit IgG (goat ...
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bioRxiv - Molecular Biology 2021Quote: ... after which the proteins were transferred to Immobilon P membranes (Merck Millipore). After blocking the membrane with 5% skimmed milk in TBS-T buffer solution for 1 h at RT or overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... protein was concentrated using a centrifugation filter (3K, Amicon, Merck, Darmstadt, Germany) up to a final concentration of 5 mg/mL and stored at −80°C.
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bioRxiv - Biophysics 2019Quote: ... Protein complex was concentrated with Amicon® Ultra Centrifugal Filters (Merck Millipore) and further purified on a Superdex 200 10/300 GL size-exclusion column (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were concentrated by ultrafiltration using Amicon Ultra centrifugal filters (Merck Millipore) with respective molecular weight cut offs ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to a PVDF membrane (Merck Millipore cat. No. IPFL00010) after which the membrane was blocked using Odyssey Blocking buffer (PBS ...
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bioRxiv - Biophysics 2021Quote: ... proteins were separated from salts and concentrated using Amicon Ultra 15 (Merck). Full length DnaK chaperone was purified by Ni2+-NTA column ...
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bioRxiv - Neuroscience 2020Quote: ... Thirty μl of PureProteome™ Protein A/G Mix Magnetic Beads (Merck) was washed in TBST and then incubated with 5 μl anti-HA antibody for 60 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were transferred onto a PVDF membrane (Immobilon-P, Merck Millipore) and the membranes were blocked with TBS containing 0.1% Tween 20 and 5% non-fat dry milk for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... mutant proteins to the cyanogen bromide (CNBr) activated sepharose 4b resin (Merck). Resin was activated by swelling it into 1 mM HCl according to the manufacturer’s instructions and equilibrated with a coupling buffer (20 mM sodium phosphate pH 8 ...
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bioRxiv - Microbiology 2022Quote: ... protein extracts were purified with 10 kDa Amicon filters (Merck, Darmstadt, Germany). Protein concentration was determined with Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... All protein concentrations were determined using a Direct Detect Infrared Spectrometer (Merck).
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bioRxiv - Neuroscience 2022Quote: ... IgG from whole blood was purified using protein A Sepharose beads (Merck). Serum was diluted 1:2 in PBS ...
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bioRxiv - Synthetic Biology 2024Quote: ... Aqueous solutions without protein components were filtered using Millex-GV filters (Merck), Novec HFE-7500 (3M ...
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bioRxiv - Developmental Biology 2024Quote: ... Following gel-electrophoresis proteins were transferred to a PVDF membrane (Merck, IPVH00010) using a semi-dry transfer cell (Biorad Transblot SD) ...
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bioRxiv - Cell Biology 2023Quote: ... lysates with equal protein concentration were incubated with Rabbit IgG (Merck, control) and 50 μg of Stwl antibody overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred to an Immobilon-PVDF membrane (Merck, Cat.N. IEVH00005) at 100V for 75 minutes in transfer buffer (20% methanol ...
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bioRxiv - Microbiology 2023Quote: ... followed by the transfer of protein onto the PVDF membrane (Merck Millipore) using a wet-tank blotting system (Bio-Rad) ...