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Citations for Merck :
501 - 550 of 1274 citations for Recombinant Human APCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... protein was concentrated using a centrifugation filter (3K, Amicon, Merck, Darmstadt, Germany) up to a final concentration of 5 mg/mL and stored at −80°C.
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bioRxiv - Biophysics 2019Quote: ... Protein complex was concentrated with Amicon® Ultra Centrifugal Filters (Merck Millipore) and further purified on a Superdex 200 10/300 GL size-exclusion column (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were concentrated by ultrafiltration using Amicon Ultra centrifugal filters (Merck Millipore) with respective molecular weight cut offs ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to a PVDF membrane (Merck Millipore cat. No. IPFL00010) after which the membrane was blocked using Odyssey Blocking buffer (PBS ...
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bioRxiv - Biophysics 2021Quote: ... proteins were separated from salts and concentrated using Amicon Ultra 15 (Merck). Full length DnaK chaperone was purified by Ni2+-NTA column ...
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bioRxiv - Neuroscience 2020Quote: ... Thirty μl of PureProteome™ Protein A/G Mix Magnetic Beads (Merck) was washed in TBST and then incubated with 5 μl anti-HA antibody for 60 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were transferred onto a PVDF membrane (Immobilon-P, Merck Millipore) and the membranes were blocked with TBS containing 0.1% Tween 20 and 5% non-fat dry milk for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... mutant proteins to the cyanogen bromide (CNBr) activated sepharose 4b resin (Merck). Resin was activated by swelling it into 1 mM HCl according to the manufacturer’s instructions and equilibrated with a coupling buffer (20 mM sodium phosphate pH 8 ...
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bioRxiv - Microbiology 2022Quote: ... protein extracts were purified with 10 kDa Amicon filters (Merck, Darmstadt, Germany). Protein concentration was determined with Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... All protein concentrations were determined using a Direct Detect Infrared Spectrometer (Merck).
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bioRxiv - Neuroscience 2022Quote: ... IgG from whole blood was purified using protein A Sepharose beads (Merck). Serum was diluted 1:2 in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred to an Immobilon-PVDF membrane (Merck, Cat.N. IEVH00005) at 100V for 75 minutes in transfer buffer (20% methanol ...
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bioRxiv - Microbiology 2023Quote: ... followed by the transfer of protein onto the PVDF membrane (Merck Millipore) using a wet-tank blotting system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: Immunoprecipitation was performed using Protein-A Sepharose from Staphylococcus aureus (Merck Millipore) and anti-TKS4 antibody (Lányi et al ...
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bioRxiv - Biochemistry 2023Quote: ... The peak protein pool was concentrated by Amicon Ultra-4 (Merck, #UFC801024), loaded onto a 15–35% glycerol gradient in 5 mL of buffer H containing 0.1 M NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein aggregates were removed by centrifugation (0.45 um filter plate, Merck, #MSHVN4550). From the soluble fraction the protein concentration was determined (BCA assay ...
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bioRxiv - Immunology 2023Quote: ... Glycans were extracted with a polyvinylidene-fluoride protein-binding membrane (Merck Millipore). Digested glycans were analyzed by HILIC-UPLC as before (51 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... rabbit polyclonal against Cytoskeleton-associated protein 4 (P63; Merck, Germany; cat # HPA001225), and sheep polyclonal against Trans-Golgi network integral membrane protein 2 (TGN46 ...
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bioRxiv - Immunology 2023Quote: Carrier proteins (HEL, OVA, HSA, BSA and MSA) were purchased commercially (Merck) and dissolved in 0.1 M NaCO3 (pH = 8 ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were detected using α-HA 3F10 antibody (Merck, 11867423001, 1:4000), α-Flag M2 antibody (Merck ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 μL of protein RIPA lysis buffer (The Merck Group, Darmstadt, Germany) with proteinase (Halt™ Protease ...
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bioRxiv - Bioengineering 2023Quote: ... Wells were then blocked with protein block (3% BSA fraction V (Merck) or 10% FBS ...
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bioRxiv - Biochemistry 2023Quote: ... His tagged proteins were labeled using Ni-NTA-ATTO 488 (Merck Group) at a ratio of ATTO/protein = 1/500 in MN buffer (50 mM MES pH 6 ...
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bioRxiv - Molecular Biology 2023Quote: ... protein detection was carried out with Immobilon Crescendo Western HRP substrate (Merck) using ChemiDoc™ MP System and images were analyzed using Image Lab™ Software (Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: ... and the protein digests were washed twice with water saturated ethylacetate (Merck). Desalting of the proteolytic peptide sample was performed on a C18 solid phase extraction column (‘home-made ZipTip’ from a 15G punch from Empore™ octadecyl C18 47 mm extraction disks (3M ...
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bioRxiv - Cell Biology 2023Quote: ... lysates with equal protein concentration were incubated with Rabbit IgG (Merck, control) and 50 μg of Stwl antibody overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Bound proteins were analysed by mPAGETM 4-20% Bis-Tris gels (Merck) and Coomassie staining ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were electrotransferred to Immobilon-FL polyvinylidene difluoride (PVDF) membranes (Merck Millipore) and blocked with Intercept Blocking Buffer (LI-COR Biosciences ...
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bioRxiv - Genomics 2023Quote: ... Proteins were visualised using Immobilon Forte HRP substrate (Merck Millipore, MA, USA) and a Fusion FX Chemi Imager (Vilber Lourmat ...
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bioRxiv - Cell Biology 2023Quote: ... Protein levels were detected using chemiluminescent Clarity Western ECL reagent (Merck; GERPN2236) and developed using ImageQuant LAS 4000.
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bioRxiv - Molecular Biology 2023Quote: ... Protein aggregates were removed by centrifugation (0.45 um filter plate, Merck, #MSHVN4550). From the soluble fraction the protein concentration was determined (BCA assay ...
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bioRxiv - Genetics 2023Quote: ... Protein lysate was prepared in Radio-immunoprecipitation assay (RIPA) buffer (Merck Millipore) with a protease inhibitor cocktail (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... Following gel-electrophoresis proteins were transferred to a PVDF membrane (Merck, IPVH00010) using a semi-dry transfer cell (Biorad Transblot SD) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Aqueous solutions without protein components were filtered using Millex-GV filters (Merck), Novec HFE-7500 (3M ...
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bioRxiv - Microbiology 2024Quote: ... the separated proteins were transferred onto Immobilon-P PVDF membrane (Merck Millipore). The membrane was blocked with 10% skim milk in Tris Buffered Saline ...
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bioRxiv - Physiology 2020Quote: ... Insulin concentration was determined using the MILLIPLEX® MAP Human Metabolic Hormone Magnetic Bead Panel (Merck KGaA, Darmstadt, Germany) according to the manufacturer s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Purified human integrin α5β1 and anti-α5 (MAB1956Z) and anti-α5β1 (MAB1999) antibodies were from Merck (Kenilworth, NJ, USA). Volociximab was from Novus Biologicals (Littleton ...
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bioRxiv - Cell Biology 2021Quote: The digoxigenin-labeled antisense probe targeting bases 32-500 of human GNRH1 mRNA (NM_001083111.2) was transcribed in the presence of digoxigenin-11-UTP (Merck Millipore) in a reaction mixture containing linearized cDNA template (1 µg) ...
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bioRxiv - Biochemistry 2020Quote: ... was equilibrated with increasing concentrations of NAb (30 pM–1.0 µM) in the presence of 90% heat-inactivated human serum (H5667, Merck) at a temperature of 4 °C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... was mixed with unlabeled SARS-CoV-2 spike S1 at increasing concentrations (100 pM–3.2 µM) in the presence of 90% heat-inactivated human serum (H5667, Merck) and incubated at 4 °C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... CD3+ T cells were cultured in TexMACS Medium (Miltenyi Biotech) supplemented with 5% heat-inactivated human AB serum (Merck), 100 units/mL penicillin (Merck ...
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bioRxiv - Molecular Biology 2022Quote: ... and primers anchored in the homology arms of the HDRT specific for KI at the human TRAC locus (Merck). PCR amplicons (typically 2.5 mL ...
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bioRxiv - Cell Biology 2023Quote: 293T human embryo kidney (HEK293T) cells were grown in Dulbecco’s modified Eagle’s medium (4.5 g/l glucose) (Merck, Dorset, UK) enriched with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biophysics 2021Quote: ... The labeled protein concentration was measured using the Direct Detect spectrometer (Merck Millipore). The degree of labeling was calculated as the ratio of the dye concentration and the protein concentration ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were transferred onto an Immobilon FL PVDF membrane (Merck Millipore, Darmstadt, Germany), probed with antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration of each lysate was determined using a BCA assay (Merck, 71285).
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bioRxiv - Immunology 2021Quote: ... The deglycosylated protein was desalted and concentrated using Amicon filter (Merck Millipore, USA). The native protein and enzyme treated proteins were separated through SDS-PAGE and the proteins were transferred to the Nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio Rad) ...
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bioRxiv - Microbiology 2021Quote: ... The protein was concentrated using an Amicon Ultra 10 K device (Merck Millipore), loaded on a size exclusion column [ENrich SEC 650 10 x 300 Column (Bio-Rad)] ...
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bioRxiv - Microbiology 2021Quote: ... Purified protein was further concentrated using Amicon Ultra Centrifugal Filter Units (Merck Millipore).
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bioRxiv - Immunology 2021Quote: ... proteins were concentrated on a 0.5 mL 10 kDa filter (UFC501024, Merck-Millipore) by ultracentrifugation and purity was controlled by SDS-PAGE.