Labshake search
Citations for Merck :
501 - 550 of 705 citations for Mouse ONECUT3 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 1:1000 anti-glutamine synthetase (monoclonal mouse, Merck Milipore, MAB 302, clone GS-6) or 1:200 anti-oxytocin receptor (polyclonal rabbit ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used: mouse anti-NP1 (OT staining; 1:2000; Merck; MABN844), rabbit anti-cfos (1:500 ...
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bioRxiv - Biophysics 2024Quote: ... We utilized either the monoclonal anti-vinculin antibody produced in mouse (Merck, Ref: V9131) at 1:200 or the YAP monoclonal antibody (M01 clone 2F12 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated with the following antibodies: mouse monoclonal raised against eIF5A1 (SAB1402762, Merck), rabbit polyclonal raised against eIF5A2 (17069-1-AP ...
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bioRxiv - Neuroscience 2024Quote: ... after which they were incubated in primary antibody (mouse anti-MBP 1:500, Merck NE1019 ...
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bioRxiv - Biochemistry 2021Quote: ... These Wild Type or Mutant Type let-7a-5p plasmids were co□transfected into treated human chondrocytes cells along with NC mimics or KCNQ1OT1 mimic (Sigma□Aldrich; Merck KGaA) using Lipofectamine 6000 following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... PfCERLI1HAGlmS parasites were transfected with the an episomal cytosolic GFP expressing plasmid pHGBrHrBl-1/2 and maintenance of this plasmid was selected for using 5 μg/mL blasticidin-S-deaminase HCl (Merck Millipore). Maintenance of the SLI-TGD plasmid was selected for using 20 μM WR99210 ...
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bioRxiv - Genomics 2021Quote: Localization of resulting plasmids was assessed with immunofluorescence staining using an anti-flag antibody (F7425, Sigma Aldrich, Merck, Kenilworth, NJ, USA) in combination with Alexa 546 secondary antibody (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 1-2 µg of pcDNA5 FRT/TO plasmids containing the gene of interest using GeneJuice transfection reagent (Cat#70967, Merck Millipore) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The pNGS2-N12-BC libraries were prepared by purification from the harvested cells by using either GenElute Plasmid Miniprep (Merck, Germany) or NucleoBond Xtra Midi (TaKaRa Bio Inc. ...
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bioRxiv - Neuroscience 2020Quote: ... Then they were incubated in mouse anti-Cx35/36 antibody (MAB3045, EMD Millipore, Merck, USA) at a dilution of 1:250 in blocking solution for 48 hours ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were incubated with anti-mouse antibody (1:5,000; Merck Millipore, Germany; Cat. No. AP124P). After incubation ...
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bioRxiv - Developmental Biology 2021Quote: PLA was performed using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Merck, USA) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: The procedures for Cre-recombinase (anti-Cre-recombinase antibody; 1:500, mouse, MAB3120, Merck Millipore), and Fos-B (anti-FosB antibody 1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... using the following antibodies: mouse anti-NeuN (1:1500, Cat. # MAB377, Merck KGaA, Darmstadt, Germany), chick anti-GFP (1:2000 ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used were the following: mouse anti-NeuN (1:500, RRID: AB_2298772, Merck Millipore) or rabbit anti-RFP (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... The primary antibodies used were mouse monoclonal antibodies against actin (1:10000, Merck Millipore, MAB1501R), synaptophysin (1:5000 ...
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bioRxiv - Neuroscience 2021Quote: ... and the other for neuronal nuclei (mouse anti-NeuN monoclonal antibody (Merck MAB377, 1:200)) ...
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bioRxiv - Synthetic Biology 2020Quote: ... a 1/5000 dilution of anti-mouse IgG conjugated with peroxidase (POD; Merck, MO, USA) was used to reveal the presence of bound anti-E-tag ...
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bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal pS129 (11A5, 1:10,000) and tyrosine hydroxylase (TH, #AB152, Merck Millipore, 1:1000). Appropriate secondary Alexa Flour antibodies (Life Technologies and Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... then probed with anti-mouse or rabbit secondary antibody conjugated to HRP (Merck AP181P, AP132P) for 60 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: Mouse monoclonal antibodies used in this study were anti-β-actin (AC-15, Merck, A1978), anti-His tag (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: Mouse neuroblastoma N2a-APPswe cells were cultured in Millicell EZ SLIDE 8-well glass (Merck). After treatments cells were washed 3x with PBS for 10 minutes ...
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bioRxiv - Physiology 2023Quote: ... mouse monoclonal anti-phosphoserine conjugated to Duolink® PLA MINUS probe (DUO87012, 1:50, Merck).
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bioRxiv - Molecular Biology 2023Quote: ... and Cy3-conjugated goat anti-mouse secondary antibody (diluted at 1:200, #AP124C; Merck Millipore) for detection ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse monoclonal anti vinculin (V9131) and rabbit polyclonal anti beta actin (A2066) were from Merck. Rabbit polyclonal antibodies to ARL8b (A88749) ...
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bioRxiv - Developmental Biology 2024Quote: ... and LH were measured using the Mouse Pituitary Magnetic Bead Panel (MPTMAG-49K, Merck, USA) according to manufacturer instructions ...
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bioRxiv - Microbiology 2019Quote: ... recodonised sequence was synthesised (gBlock gene fragments, Integrated DNA Technologies) and Gibson cloned (Gibson et al., 2010) into BamHI and NdeI digested pET-28a(+) plasmid (Merck, Darmstadt, Germany). This allowed for expression of an N-terminal 6xHis tagged shortCAP recombinant protein in Escherichia coli BL21 cells under the control of T7 lac promoter ...
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bioRxiv - Immunology 2022Quote: ... coli transformed with plasmid pET42 expressing N-terminally GST- and HIS-tagged human IL-18 were grown at 37°C in Lysogeny broth (LB) medium containing kanamycin (25 μg/ml) as selection marker for the pET42a plasmid (Cat No 70561, Novagen, Merck, Overijse, Belgium). When the optical density at 600 nm (OD600 ...
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bioRxiv - Biophysics 2022Quote: ... an amber codon (TAG) was introduced using appropriate mutagenic oligonucleotides and a protocol for whole plasmid site-directed mutagenesis employing KOD polymerase (Merck Millipore, Germany) as detailed in manufacturer’s instructions and previous work (Zheng et al. ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells transfected with Flag-tagged NSP1 or vector plasmid were treated with the following compounds for 6 h: 10 μM of MG132 (Merck, Cat# 474787), 50 μM of chloroquine (CQ ...
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bioRxiv - Immunology 2022Quote: Serum insulin was determined using a rat/mouse insulin ELISA kit (Millipore, Billerica, Merck KGaA, USA) as instructed ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse monoclonal IgG2A antibody to myelin Proteolipid Protein (clone PLPC1, MAB388, MERCK-Millipore, 1:100). All slides were mounted using Mowiol mounting medium (475904 ...
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bioRxiv - Biochemistry 2021Quote: ... the primary antibody (2.0µg/mL; anti-DNA:RNA hybrid, clone S9.6; monoclonal, mouse, IgG2ak, Merck Millipore, Germany) and the secondary fluorescence-labeled anti-mouse IgG antibody (2.5 µg/mL ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated with 1:1,000 mouse anti-Rac1 (clone 23A8 05-389; Merck Millipore, USA) overnight at 4 °C and 3 h at RT and then washed 3 times with TTBS ...
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bioRxiv - Cell Biology 2022Quote: For western blot and immunofluorescence analysis following primary antibodies were used: Anti-Actin (mouse, Merck Millipore); Anti-ARP3 (mouse ...
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bioRxiv - Neuroscience 2023Quote: ... We used the primary antibody of anti-PV mouse monoclonal antibody (1:1000, MAB1572, Merck Millipore) combined with the secondary antibody of anti-Mouse polyclonal goat antibody conjugated with Alexa Fluor 647 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... and mouse anti-human TMPRSS2 (monoclonal, clone P5H9-A3, cat num: MABF2158, 1:500, MERCK, USA) for a minimum of 2 h at RT ...
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bioRxiv - Bioengineering 2023Quote: ... the retinal sections were stained with different antibodies: mouse anti– rhodopsin 1D4 (1:1500, 1D4, Merck), rabbit anti-M-opsin (1:300 ...
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bioRxiv - Biochemistry 2023Quote: Mouse neuroblastoma N2a-APPswe cells were cultured in Millicell EZ SLIDE 8-well glass slides (Merck). After treatments cells were washed with PBS (3 times ...
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bioRxiv - Neuroscience 2023Quote: ... 10 µg of hGCase-1/17 or hGCase-1/23 or mouse IgG (Merck Millipore, #NI03) were coupled to 50 µl of Protein A magnetic Dynabeads in 200 µl Ab Binding and Washing Buffer for 30 minutes at RT on a rotating wheel ...
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bioRxiv - Molecular Biology 2023Quote: ... and Alexa Fluor 488-conjugated goat anti-mouse antibody (diluted at 1:200, #AP124C; Merck Millipore) as the secondary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... We dissected hippocampi from newborn mouse brain in ice-cold dissection media (HBSS (Merck Life Sciences) supplemented with 0.1 % w/v glucose ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: mouse monoclonal antibody against actin (1:10,000, Merck Millipore, MAB1501R); mouse monoclonal antibody against PrP (POM1 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then first incubated with mouse anti-PSD95 antibodies (1:100; Merck-Millipore, Cat. #MAB1596) overnight at 4°C ...
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bioRxiv - Genomics 2021Quote: ... These pX330-mC plasmid DNAs and donor DNA plasmid were isolated using FastGene Plasmid Mini kit (Nippon Genetics, Tokyo, Japan) and filtered using MILLEX-GV® 0.22 μm filter unit (Merck Millipore, Darmstadt, Germany) for microinjection ...
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bioRxiv - Cancer Biology 2022Quote: ... 293T cells were co-transfected with second generation LV packaging plasmids pMD2.G and pCMV-dR8.74 (plasmidFactory, Bielefeld, Germany) and CAR transfer vectors using GeneJuice® Transfection Reagent (Merck-KGaA, Darmstadt, Germany) according to manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... 250 ng of pTOPflash (wt-TCF) or pFOPflash (mutated-TCF binding site, negative control) reporter plasmids (TOPflash luciferase reporter assay, Merck Millipore, Darmstadt, Germany) were transfected with Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cancer Biology 2019Quote: ... GST was labeled with mouse Anti-GST Tag monoclonal antibody (05-782, Merck Chemicals; 2.5 μg/ml). To account for variations in the amount of fusion protein ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used in this study were: mouse monoclonal antibodies against Centrin (1:500 for immunofluorescence; Merck Millipore catalogue number 04-1624 ...