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Citations for Merck :
5101 - 5150 of 5376 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Biochemical studies of blood serum samples were carried out on VITALAB SELECTRA 2 in an automatic analyzer (Merck, Germany) using commercial kits (DIASYS Diagnostic Systems GmbH ...
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bioRxiv - Cell Biology 2021Quote: ... The floated sections were rinsed briefly with 100 mM maleate buffer (pH 7.5) and blocked for 30 minutes against non-specific antibody binding with 2% blocking reagent (Merck) in maleate buffer ...
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bioRxiv - Biophysics 2022Quote: ... the sample was placed on ice and the proteinase activity was quenched with 2 mM phenylmethylsulfonyl fluoride (Merck, P7626) in DMSO.
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bioRxiv - Immunology 2022Quote: NPC cellular phenotypes were induced in RAW 264.7 and J774.A MΦ by treating with 2 μg/ml U18666A (Merck) for 30h or 72h respectively ...
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bioRxiv - Cell Biology 2022Quote: ... resolubilized in 25 µL of 0.05% trifluoroacetic acid in 98:2 H2O/acetonitrile (v/v) and filtrated through a 0.2 µm spin filter (Merck Millipore Ultrafree®-MC ...
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bioRxiv - Microbiology 2020Quote: The TLC was performed by spotting 2 μL of enzymatic reaction on a silica gel 60 F454 plate (Merck), the separation was carried out in butanol ...
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bioRxiv - Cell Biology 2023Quote: ... To induce the expression of the GOI the cells were treated with doxycycline (2 μg/ml, Merck, Cat.N. 3072) for the duration of the experiment ...
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bioRxiv - Molecular Biology 2023Quote: ... Peak fractions containing SETDB1 Triple Tudor protein were pooled and concentrated to 2 mL in Amicon Ultra-15 concentrators 10,000 molecular weight cut-off (Merck Millipore ...
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bioRxiv - Biophysics 2023Quote: ... in digestion buffer (800 mM guanidine HCl, 2 mM CaCl2, 0.5 % Triton X-100, in 50 mM TRIS, #8382J008706, Merck), overnight at 50°C ...
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bioRxiv - Genomics 2023Quote: ... a stock solution of 2 mg/mL colistin sulfate (Sigma-Aldrich Merck KGaA, Germany; 100 mg, 15,000 U/mg) was prepared and aliquots were stored at −80 °C ...
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bioRxiv - Immunology 2023Quote: ... Afterward, the cells were washed twice in R10 media (RPMI-1640 with 10% FBS, 2 mM glutamine (G7513, Merck), 1% penicillin-streptomycin (P0781 ...
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bioRxiv - Microbiology 2023Quote: ... were incubated for 2 h at room temperature with the respective recombinant glycosyltransferase enzymes (6.3 µg/ mL) and UDP-GlcNAc (2 mM, Merck) in glycosylation buffer (15 mM HEPES ...
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bioRxiv - Neuroscience 2022Quote: ... was used in a concentration of 20 µM and autocamtide-2 related inhibitory peptide II (AIP-II; Merck Millipore) at 4 µM.
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bioRxiv - Molecular Biology 2022Quote: ... samples were cleaned with a solid phase extraction method using 2 layers of C18 Empore™ SPE Disks (Merck) followed by 2 layers of SCX strong cation exchange Empore™ SPE Disks (Merck) ...
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bioRxiv - Neuroscience 2024Quote: ... concentrated using 100-kDa molecular weight cut-off (MWCO) filters Centricon plus-20 and Centricon plus-2 (Merck-Millipore), aliquoted and stored at - 80°C ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated in xylene for at least 2 min and then covered with mounting solution Entellan ® (Merck). Images were acquired at a S360 Hamamatsu slide scanner using a 40x objective ...
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bioRxiv - Microbiology 2023Quote: ... 100 ml of the cell suspension at an OD660 of 0.6 was harvested by centrifugation at 5,000×g for 10 min at 4°C and resuspended in 2 ml of BugBuster (Merck), and 50 μg/ml lysozyme and 1 mM phenylmethanesulfonyl fluoride (PMSF ...
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bioRxiv - Neuroscience 2024Quote: Whole iPSC-brain organoids were permeabilized with 0.5% Triton X-100 in blocking solution with 2% Bovine Serum Albumin (BSA; Merck) in PBS for 3 hours at RT ...
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bioRxiv - Biochemistry 2023Quote: ... was performed on 4 and 2.5 mg protein lysates for timsTOF optimization and dual-probe analyses respectively in 2 M urea (Merck) in 1× 50 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2023Quote: ... The bound protein complexes were washed thrice with NETN buffer and then eluted with 2 mg/mL biotin (Merck) for 90 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatant was collected as control and the beads were thoroughly washed 3 x with TBS/Tween 0.2% after which bound proteins were eluted by adding hot reducing SDS sample buffer containing 2-mercaptoethanol (Merck) and boiling for 5 min at 99 °C ...
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bioRxiv - Plant Biology 2024Quote: A pTYB2-based vector for the expression of PSM polypeptides and a pCDF-based vector that contained heme oxygenase 1 and phytochromobilin synthase genes for the synthesis of PΦB (Muramoto et al., 1999; Kohchi et al., 2001) were transformed into the Escherichia coli Rosetta 2 strain (Merck). E ...
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bioRxiv - Developmental Biology 2022Quote: ... the obtained 2-cell stage embryos were transferred to a fresh drop of KSOM (Merck, cat. # MR-107-D). The IVF with b6d2F1 oocytes and b6casF1 has on average a 2-cell development (fertilization ...
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bioRxiv - Plant Biology 2022Quote: ... except that the resuspension of protein precipitate was in modified denaturing urea buffer (8 M urea, 2% SDS, 100 mM HEPES-KOH pH 7.4, 10 mM EDTA and protease inhibitor [Calbiochem, now Merck, https://www.merckmillipore.com] containing 50 mM N-ethylmaleimide (NEM ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were stained with DAPI before mounting with Mowiol (12 mL of 0.2 M Tris buffer pH8.5, 6 mL distilled water, 6 g glycerol, 2.4 g Mowiol 4-88, Merck Millipore).
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bioRxiv - Plant Biology 2023Quote: ... dried and transferred in a grid pattern onto square plates containing 50 ml of MS/2 medium (0.5× Murashige and Skoog salts [Merck Sigma-Aldrich] ...
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bioRxiv - Cancer Biology 2023Quote: ... They were further incubated with a 2× volume of 0.25× diluted TES buffer with a trace amount of benzonase nuclease (Merck) at 4°C for 45 min.
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bioRxiv - Microbiology 2024Quote: ... coli SM10 pCVD442-up-dotB-down and the SP-1303 ΔdotB mutant by mixing equivalent volumes of adjusted overnight (O/N) liquid culture to OD=2 on a 0.45 µm filter (peel corner, MERCK) positioned on a LB agar plate for 6 h at 28°C ...
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bioRxiv - Neuroscience 2024Quote: ... 2 washes with 1X HBSS and an incubation in a trypsin-based digestion solution containing DNAse I (Merck, D5025) for 15 minutes at room temperature (RT) ...
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bioRxiv - Molecular Biology 2024Quote: ... On-beads trypsin digestion was then performed by resuspending the beads in 200 µL of Digestion Buffer supplemented with 2 µL of 0.5 µg/µL Sequencing Grade Trypsin (Merck). Samples were incubated overnight at 37°C with constant agitation at 750 rpm ...
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bioRxiv - Synthetic Biology 2024Quote: ... Functional DROP-CARs were selected based on inducible GFP expression from the genomic IL-2 site and by HER2 binding (2.5 µg/mL soluble HER2 antigen (Merck) and subsequent 1:200 APC-labeled anti-HER2 antibody (Biolegend)) ...
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bioRxiv - Neuroscience 2024Quote: ... The blocking solution was then replaced with another consisting of 2 µL mouse anti-NeuN antibody (Merck Millipore, MAB377) and 0.5 µL of rabbit anti-GFAP antibody (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... Sections or cells were pre-incubated in blocking buffer containing 2–10% normal donkey serum (Merck Millipore, Burlington, MA) and 0.1% Triton X-100 at RT for 1 h and incubated overnight at 4 °C with primary antibodies and subsequently incubated with secondary antibodies conjugated with Alexa Fluor 488 ...
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bioRxiv - Neuroscience 2024Quote: ... The amplicons were resolved by electrophoresis in a 2% agarose gel stained with ethidium bromide (0.5 ug/ml, Merck), and MW markers 100 bp DNA ladder (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... The cleared lysate was transferred to new 2-mL tubes containing 70 µL Anti-FLAG M2 affinity gel (Merck) pre-eluted with
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bioRxiv - Cancer Biology 2024Quote: ... A538.5) for 2 min after which slides were mounted using Eukitt quick hardening mounting medium (Merck, catalog no. 03989). Slides were then scanned using Leica DM6 B microscope and Leica Aperio Versa 8 slide scanner with 20x zoom ...
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bioRxiv - Cell Biology 2020Quote: U-2-OS Tet-On cell lines expressing eGFP-TopBP1 WT were grown under standard conditions (37°C, 5% CO2) in modified McCoy’s 5a medium (Merck-Sigma-Aldrich, M9309) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Microbiology 2020Quote: ... HPLC analysis was performed using a Waters e2695 separation module (Waters) equipped with a LiChrospher 100 RP-18 column (250 × 4 mm, φ = 5 μm; Merck Millipore) at 50°C ...
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bioRxiv - Biochemistry 2021Quote: ... Pellets were thawn on ice and resuspended in lysis buffer (5 mL/g pellet: 50 mM Na2HPO4/KH2PO4, 150 mM NaCl, 0.2 % Tergitol™ solution (Merck, Darmstadt, Germany), and 2 mM DTT ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μl of sample was injected in 50 % B and the analysis was performed using the gradient of 15 % A and 85 % B for 3.5 min followed by an increase to 75 % A and 25 % B over 11.5 min and its continuation for 10 min with the SeQuant® ZIC®-pHILIC column (5 µm, 150 mm x 2.1 mm, Merck, USA), at a flow rate of 0.13 ml/min.
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bioRxiv - Microbiology 2020Quote: ... water was purified by a Milli-Q system (resistivity 18.2 MΩ cm at 25°C, TOC < 5 ppb, Merck Millipore, Molsheim, France). Indole-3-acetic-2,2-d2 acid (≥ 98% ...
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bioRxiv - Neuroscience 2020Quote: ... half of the medium was replaced with fresh medium containing 20 μM of 5-fluorodeoxyuridine and uridine (Sigma Aldrich, Merck, Darmstadt, Germany) every 3 days ...
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Molecular Biology 2021Quote: ... mESCs were grown on gelatin-coated plates under standard culture conditions (37°C, 5% CO2, humid) atop a ‘feeder’ layer of MitomycinC-inactivated (Merck Life Science) SNLP mouse fibroblasts and passaged upon ∼80% confluency every 2-3 days using TrypLE Express (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... 30 mL buffy coat was diluted with 5 mL DPBS (BioWhittaker®) and carefully poured over 15 mL of Histopaque® (Merck). After centrifugation (500 g ...
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bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... Protein transfer was then visualized using 0.1 % Ponceau S solution (w/v) in 5% acetic acid (Merck Life Sciences, Cat #: P7170-1L) and imaged ...
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bioRxiv - Evolutionary Biology 2023Quote: ... A small aliquot of each sample was added to Hemi medium17 with a 5-μl/ml antibiotic cocktail (P4083, Merck, Darmstadt, Germany) and incubated under dark conditions at 19–20 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: CC strips were dissected and then transferred to microtiter plate wells containing 5 μM bis-N-methylacridinium nitrate (Lucigenin; Merck, Darmstadt, Germany), some strips were stimulated with NADPH (100 μM ...
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bioRxiv - Bioengineering 2023Quote: ... two thirds of the medium was removed from all wells and T cells were treated with 5 ng/mL polybrene (Merck, Darmstadt, Germany). Lentiviral particles (MOI=3 ...