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Citations for Merck :
5001 - 5050 of 5749 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... K2HPO4 2.9 g/L, VWR; di-ammonium citrate 0.7 g/L, Sigma-Aldrich; sodium acetate 0.26 g/L, Merck; glucose 1% (w/v), Merck ...
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bioRxiv - Genomics 2023Quote: ... a ∼20%-confluent wild-type iPSC culture in a 12-well plate containing 1 ml/well of Essential 8 supplemented with 10 µM ROCK inhibitor (Merck, cat# Y0503) was co-transfected with the pZT-C13-L1 and pZT-C13-R1 plasmids (Cerbini et al ...
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bioRxiv - Microbiology 2023Quote: ... K2HPO4 2.9 g/l, VWR; di-ammonium citrate 0.7 g/l, Sigma-Aldrich; sodium acetate 0.26 g/l, Merck; glucose 1% (w/v), Merck ...
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bioRxiv - Molecular Biology 2023Quote: ... Media was concentrated further to a final volume of 1-2 ml using 10 KDa Amicon spin filters (Merck Millipore, MA, USA) by centrifugation at 3,500 g ...
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bioRxiv - Biochemistry 2023Quote: Human osteosarcoma SJSA-1 cells were purchased from ATCC (Manassas, VA, USA) and cultured with RPMI 1640 medium (Sigma Aldrich, Merck, Germany), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2022Quote: ... GFP signals were amplified by immunostaining with polyclonal rabbit anti-GFP (used at 1:500, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany, SAB4301138). 40-1a (used at 1:50 ...
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bioRxiv - Biophysics 2023Quote: ... Amphipol-reconstituted P2X4 was pooled and concentrated to approximately 1 mg/mL using an Amicon Ultra 50 kDa cutoff (Merck Millipore, USA). All steps were performed at 4 °C.
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bioRxiv - Plant Biology 2023Quote: ... Sections were rinsed 6 times in MTSB and incubated with 1 µg/mL Calcofluor white (Fluorescent Brightener 28 disodium salt solution, Merck, in MTSB). After rinsing ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% glycerol) supplemented with 1 mg/ml lysozyme and one tablet of SIGMAFAST Protease Inhibitor Cocktail Tablets EDTA-Free (Merck KGaA, Germany), and disrupted by sonication (10 cycles of 30 sec at 40 % intensity in ice ...
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bioRxiv - Cell Biology 2024Quote: ... Base elastomer was thoroughly mixed with curing agent at a ratio of a ratio of 10:1, before degassing in a vacuum desiccator (Scienceware, 999320237) with a KNF Laboport N96 diaphragm vacuum pump (Merck, Z675091-1EA). A Si wafer with positive features was placed in a disposable aluminium dish (VWR ...
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bioRxiv - Bioengineering 2024Quote: ... The scaffold was then rinsed three times with PBS and incubated with Alexa Fluor™ 555 conjugated secondary goat anti-mouse IgG antibody (1:100; Merck, Germany) and CF™ 633 conjugated secondary donkey anti-rat IgG antibody (1:100 ...
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bioRxiv - Molecular Biology 2024Quote: A FLAG-tagged or HA-tagged cDNA fragment of DDX43 was amplified by RT-PCR from total RNA extracted from silkworm ovaries and cloned into the pIEx-1 vector (Merck Millipore/Novagen) or the pIExZ vector using an In-Fusion cloning kit (Takara) ...
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bioRxiv - Microbiology 2024Quote: ... The chlorine concentrate solution was prepared by addition of a NaOCl tablet (B-Care Chemicals, Belgium) to 1 L ultrapure water (Milli-Q, Merck Millipore, Germany). The amount of free chlorine was quantified with the Pocket Colorimeter II (Hach,Belgium).
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bioRxiv - Cell Biology 2024Quote: ... hTERT-RPE1-Flp-In-Parental cells were co-transfected with a 10:1 ratio of pOG44 and either pCDNA5 FRT/TO-NeoR-HA-VPS35-WT or -[D620N] using Genejuice (Merck Millipore; 70967), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Infection foci were visualised using the appropriate secondary antibody coupled with horseradish peroxidase (HRP; 1:5000, Sheep Anti-Mouse IgG A5906, Sigma-Aldrich, Merck, Germany) and TrueBlueTM chemical (SeraCare ...
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bioRxiv - Microbiology 2024Quote: ... Actin was detected with monoclonal mouse antibody coupled with horseradish peroxidase (HRP; 1:75,000; A3854, Sigma-Aldrich, Merck, Saint-Quentin Fallavier, France). Membranes were exposed to the appropriate peroxidase-conjugated secondary antibodies (1:5000 goat anti-guineapig IgG A108P or 1:10000 sheep anti-mouse IgG A5906 ...
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bioRxiv - Microbiology 2024Quote: ... Purification and concentration of □ ERS-1 was carried out with Amicon® Ultra-15 centrifugal filter with Ultracel100 kDa membrane (Merck Millipore) (Sun et al. ...
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bioRxiv - Genetics 2024Quote: ... The cells were first arrested at G1 using 3.0 µg mL-1 α-factor grown at 25°C (Merck, Cat. No. T6901). After 90 min ...
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bioRxiv - Plant Biology 2024Quote: Visualization of intracellular and apoplastic ROS in guard cells was performed using 20 µM H2DCFDA or 100 µM peroxy orange 1 for H2O2 (PO1; Merck Sigma-Aldrich), and 100 µg/ml Oxyburst Green H2HFF-BSA (Thermo Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were resuspended in 1 mL PBS containing protease inhibitor cocktail (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail; Merck, #11836153001) (PBSI ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: For GC-MS analysis all the samples were derivatized with N,O- Bis(trimethylsilyl) trifluoroacetamide with 1% trimethylchlorosilane (BSTFA) purchased from Merck (Darmstadt, Germany).
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bioRxiv - Biophysics 2024Quote: ... The membrane pellet was resuspended in extraction buffer (25mM MOPS-NaOH, 150mM NaCl, 50mM EDTA, 10mM DTT, 0.1 mg/ml lysozyme, 1% lauroylsarcosine (Merck, Cat. No. L5777), pH 7.0 ...
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bioRxiv - Bioengineering 2024Quote: ... The extracts were prepared by incubating the formulations (1 ml, prepared as detailed above) in 3.3 ml DMEM HG medium (Merck, Cat. No. D6429) for 24 h at 37°C at a surface-to-volume ratio of 3 cm2/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... Chromatin was then sheared either by passaging 10 times through a 27G needle attached to a 1 ml syringe or by the addition of Benzonase® Nuclease (Merck, UK). Needle-sheared nuclear fractions were centrifuged at 13,000 x g for 10 minutes (4°C ...
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bioRxiv - Cell Biology 2024Quote: ... HaCaT KCs were cultured for 4 hours after seeding in DMEM supplemented with 10% FBS (HyClone) and 1× antibiotic–antimycotic mixed stock solution (Nacalai Tesque) on 3.0-µm-pored cell culture inserts (Merck KGaA, Darmstadt, Germany). The inserts were pre-coated with CELLstart (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Slices were then stored at 4°C in a PBS-solution containing 0.05% of Proclin (Merck, Darmstadt, Germany) until further processing.
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bioRxiv - Biophysics 2020Quote: ... Eluted fractions containing monomeric H57-scFV were concentrated with 10 kDa Amicon® Ultra-4 centrifugal filters (MERCK), treated with a 3C protease (71493 ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.2 buffer were concentrated to 10 mg/ml using the Amicon Ultra-4 Centrifugal Filter Unit (Merck/Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... The Bruce-4 cell line was maintained in DMEM supplemented with 15% FCS and ESGRO (Merck Millipore, UK) on a 0.1% gelatine-coated dish at 10% CO2.
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bioRxiv - Molecular Biology 2021Quote: ... treated with PBS-formaldehyde (4%) for 20 mins at room temperature and stained with crystal violet stain (Merck). After extensive washing ...
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bioRxiv - Pathology 2022Quote: ... trans-cardial perfusion was performed into the mice and rats with PBS followed by 4% paraformaldehyde (PFA, Merck). The kidneys were harvested and the samples were post-fixed in 4% PFA at 4°C for 90 min ...
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bioRxiv - Bioengineering 2022Quote: ... Sliced sections were permeabilized and blocked with 0.1% Tween-20 and 4% donkey serum (Merck Millipore, Burlington, MA) in Tris-buffered saline ...
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bioRxiv - Cell Biology 2022Quote: ... coli cells using 0.2 mM IPTG for 4 hours at 30 °C and purified on flag beads (Merck) and eluted using flag peptide buffer (200 µg/ml flag peptide in 10 mM Tris-HCL ...
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bioRxiv - Cell Biology 2022Quote: ... lysates were incubated overnight under rotation at 4°C with ANTI-FLAG® M2 Affinity Gel beads (Merck) to pull down FLAG-containing SNAP-GLP-1R ...
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bioRxiv - Neuroscience 2021Quote: ... Protein wet transfer was performed overnight at 4°C using an Immobilon®-FL PVDF membrane (Merck™). Next day ...
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bioRxiv - Biochemistry 2021Quote: ... were incubated overnight at 4°C with rotation with 20 µg/ml anti-FLAG M2 antibody (Merck, F3165) and 50 µl/ml of pre-washed Pierce magnetic protein A/G beads (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... fixed with paraformaldehyde (4% w/v (Applichem) in phosphate-buffered solution (PBS)) and used for Giemsa-staining (Merck) to calculate the number of perivascular neutrophils ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were harvested in 4°C RIPA buffer with protease inhibitor (cOmplete, EDTAfree Protease Inhibitor Cocktail, Merck) and homogenised by sonication for 30 sec ...
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bioRxiv - Cell Biology 2022Quote: E10.5 embryos were fixed in 4% PFA/PBS for 1.5 h followed by incubation in 15% sucrose (Merck)/PBS for 2 h and overnight incubation in 30% sucrose/PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... The Swi2C-Swi5 complex was concentrated using an Amicon Ultra-4 (30 k) centrifugal filter (UFC803008, Merck Millipore). After cleavage ...
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bioRxiv - Molecular Biology 2022Quote: ... The Swi2CS-Swi5 complex was concentrated using an Amicon Ultra-4 (10 k) centrifugal filter (UFC801008, Merck Millipore).
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bioRxiv - Molecular Biology 2022Quote: ... The Swi2S-Swi5 complex was concentrated using an Amicon Ultra-4 (50 k) centrifugal filter (UFC805008, Merck Millipore), applied to a 5 mL HiTrap Heparin column ...
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bioRxiv - Cell Biology 2022Quote: ... and concentrated at 4 °C approximately 300 times using a 10 kDa Centricon Plus-70 centrifugal unit (Merck Millipore ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: HepaRG cells were washed twice with 100 μl PBS pH 7.4 and fixed with paraformaldehyde solution (4% in PBS pH 7.4, Merck) for 40 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Fractions containing monomeric and labeled 14.4.4 scFVs were concentrated with Amicon Ultra-4 centrifugal filters (10 kDa cut off, Merck) to arrive at a protein concentration between 0.2 mg ml-1 and 1 mg ml-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... pooled according to purity and afterwards concentrated using Amicon Ultra-4 centrifuge filters (30 kDa cutoff, Merck Millipore). The purified octamers were validated by SDS-PAGE ...
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bioRxiv - Biophysics 2023Quote: ... Peak fractions were pooled and concentrated in an Amicon Ultra-4 Ultracell 30kDa centrifugal filter (Merck-Millipore #UFC803024). Aliquots were snap frozen and stored at −80 °C ...
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bioRxiv - Biophysics 2023Quote: ... Peak fractions were pooled and concentrated in an Amicon Ultra-4 Ultracell 30kDa centrifugal filter (Merck-Millipore #UFC803024). Aliquots were snap frozen and stored at −80°C ...
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bioRxiv - Neuroscience 2023Quote: ... and then protein was loaded into Superdex 200 and concentrated using Amicon Ultra-4 (3K cut off, Merck). The labeling percentage was calculated according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... at 4 °C for 30 s and concentrated in a 100 kDa Amicon Ultra centrifugal filter (Merck Millipore), which was equilibrated with 1% GDN in IP buffer ...