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Citations for Merck :
451 - 500 of 1071 citations for Recombinant Swine OSM Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated with first and secondary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were resolved in SDS-PAGE gels and electrotransferred onto nitrocellulose membranes (Merck). After incubation with the appropriated primary antibody ...
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bioRxiv - Microbiology 2023Quote: ... Then the protein sample was concentrated using an Amicon Ultra centrifugal tube (Merck) with a buffer change to phosphorylation buffer 2X (100 mM Tris pH 8 ...
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bioRxiv - Plant Biology 2023Quote: ... after concentrating the protein (Amicon® Ultra MWCO 30 kDa, Merck, Darmstadt, Germany), a second GST-tag purification was performed and the peak fractions were pooled and concentrated (Amicon® Ultra MWCO 10 kDa ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were blotted on the Immobilon-P PVDF 0.45 µm (MERCK, Darmstadt, Germany). PVDF membranes were blocked using the PVDF Blocking Reagent for Can Get Signal (TOYOBO) ...
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bioRxiv - Microbiology 2023Quote: ... and the protein concentration was determined by bicinchoninic acid (BCA) assay (Merck Millipore) and by UV-visible spectroscopy using an ε of 24 mM⁻¹·cm⁻¹ at 280 nm ...
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bioRxiv - Physiology 2023Quote: ... and protein concentration in the supernatant was determined by BCA assay (Merck B9643). Finally ...
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bioRxiv - Biochemistry 2023Quote: ... The 139H2 was purified with Protein G Sepharose 4 Fast Flow beads (Merck), washed with PBS ...
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bioRxiv - Physiology 2023Quote: ... a specific inhibitor targeting the alpha subunit of the Gi/o protein (Merck); U73122 ...
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bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Microbiology 2023Quote: ... The proteins were concentrated by centrifugal filters (Amicon Ultra 10K, Merck Millipore Ltd.). Protein concentrations were determined by a Pierce™ BCA Protein Assay Kit (Thermo Fischer Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were filtered using an Ultrafree 0.22 μm centrifugal filter (Merck Millipore) to remove any aggregation ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50 µM Rho-associated protein kinase (ROCK) inhibitor (Merck Millipore, SCM-075). On day 3 ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were transferred to 0.45 µm PVDF membranes (Merck Millipore, cat.no. IPVH00010). After blocking unspecific binding sites of the membrane at RT for 1 h with 5 % skim milk powder in TBS-T ...
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bioRxiv - Cell Biology 2023Quote: Caco-2 cells were co-transfected with eSpCas9-GFP protein (Merck, cat #ECAS9GFPPR) and sygRNA (Merck ...
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bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Microbiology 2024Quote: ... The pellet was resuspended in protein resuspension buffer (150 mM NaCl (Merck, USA), 5 mM EDTA (Merck ...
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bioRxiv - Plant Biology 2024Quote: ... Protein pellets were resuspended in 50 μl of 1.5% sodium deoxycholate (SDC; Merck) in 0.2 M EPPS-buffer (Merck) ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were concentrated using a 10K Amicon tube (Merck-Millipore, Darmstadt, Germany) and the buffer was changed to PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were concentrated using a 10K Amicon tube (Merck-Millipore, Darmstadt, Germany) and the buffer was changed to PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... FLAG-tagged proteins were detected using M2 anti-FLAG antibody (mouse, F1084, Merck) followed and fluorescent Anti-Mouse Alexa Fluor™ Plus 800 ...
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bioRxiv - Biochemistry 2024Quote: ... Immunoreactive proteins were visualized using Immobilon® Forte Western HRP Substrate (Merck Millipore) at the Imager Fluor-Chem M (Proteinsimple) ...
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bioRxiv - Molecular Biology 2024Quote: ... protein pellets were resuspended in 50 µl of 1.5% sodium deoxycholate (SDC; Merck) in 0.2 M EPPS-buffer (Merck) ...
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bioRxiv - Cancer Biology 2021Quote: ... and thereafter protein transferred onto 45 µm Polyvinylidene difluoride (PVDF) membrane (IPVH00010, Merck Millipore). Western blotting analysis was performed by using specific primary and secondary antibodies and signals visualized with chemiluminescence ...
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bioRxiv - Cell Biology 2020Quote: ... and nucleoproteins were extracted using a nuclear protein extraction kit (Merck Millipore, Darmstadt, Germany). Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS ...
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bioRxiv - Cell Biology 2019Quote: ... Supernatant containing Matrigel proteins was discarded and pellets were lyzed in RIPA buffer (Merck), supplied with protease and phosphatase inhibitors (Merck) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... For IP with guinea-pig antibody protein A-conjugated magnetic beads (Merck Millipore, LSKMAGA10) were used ...
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bioRxiv - Developmental Biology 2020Quote: hESCs were harvested for protein extraction by addition of CelLytic M lysis buffer (Merck), freshly supplemented with protease inhibitors (PIC ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were eluted with 20 μl of 1 mg/ml 3xFLAG peptide (Merck, F4799) for 15 min at room temperature with vigorous shaking ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were concentrated with an Amicon Ultra-15 10 kDa centrifugal filter unit (Merck). Protein concentration was determined by UV-spectroscopy.
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bioRxiv - Immunology 2021Quote: ... Supernatants were first precleared with PureProteome™ Protein A/G agarose suspension (Merck Millipore) for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... The purified proteins were then concentrated on either Amicon (Merck Millipore, MWCO 10 kDa) or Vivaspin (Sartorius ...
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bioRxiv - Genetics 2021Quote: ... Proteins separated by SDS-PAGE were transferred onto a PVDF membrane (Immobilon-E, Merck) and blocked in 5% milk in Tris-Buffer Saline (TBS ...
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bioRxiv - Pathology 2021Quote: ... proteins were transferred from the gel onto to a polyvinylidene fluoride membrane (Merck Millipore) via electroblotting at 120 V for 60 minutes in transfer buffer containing 20% methanol ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were detected using a monoclonal anti-FLAG M2 antibody (F3165, Sigma/Merck Millipore) and an HRP-conjugated polyclonal goat anti-mouse antibody (P0447 ...
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bioRxiv - Bioengineering 2021Quote: ... the cDNA of the fusion protein was cloned in pET21a (Merck KGaA, Darmstadt, Germany). For preparative overexpression the E ...
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bioRxiv - Bioengineering 2021Quote: ... and supplemented with 10 µM/ml Rho-associated protein kinase inhibitor (Y-27632, Merck) for the first day.
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bioRxiv - Cell Biology 2021Quote: ... proteins were concentrated using Amicon Ultra-4 30 kDa centrifugal filter tubes (#UFC803024, Merck) and resuspended in sterile HBSS containing 1.2 mmol/l Ca2+ ...
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bioRxiv - Microbiology 2020Quote: ... Imidazole was removed from purified protein fractions using Amicon Ultra centrifugal filters (UFC505024, Merck) and washed three times and reconstituted in buffer D (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2020Quote: ... The purified protein was desalted using the Amicon Ultra-15 Centrifugal Filter Unit (Merck) and the desalted protein was used for activity assays ...
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bioRxiv - Microbiology 2020Quote: ... 40 µg proteins were mixed with acetone (Uvasol® for spectroscopy, Merck, Darmstadt, Germany) to obtain a final concentration of 80 % acetone (v/v ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred onto a polyvinylidene difluoride (PVDF) membrane (Immobilon-PSQ PVDF membrane, Merck) by semi-dry turbo Western blot procedure (Trans-Blot Turbo Transfer System ...
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bioRxiv - Cancer Biology 2021Quote: ... An aliquot of extract containing 100 µg protein was then digested by benzonase (Merck) and precipitated by acetone ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 30 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 100 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... Protein extracts were further processed in a Microcon-30 kDa Centrifugal Filter Units (Merck) in a tempered centrifuge at 24°C ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 30 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... 10 µM Mif2 protein per condition was immobilized on anti-FlagM2 agarose beads (Merck) for 1 h at 4 °C and 1200 rpm in a thermomixer ...
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bioRxiv - Systems Biology 2022Quote: ... proteins were reduced by addition of 5 mM DTT (Merck Sigma, Cat. No. 43815) or 5 mM TCEP (Merck Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... and immunoreactive proteins were visualised using Immobilon® Forte Western HRP Substrate (Merck Millipore). Densitometric analysis was carried out using the ImageJ 1.53c software ...