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Citations for Merck :
451 - 500 of 1429 citations for Recombinant Human CD22 protein His tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Proteins were electroblotted onto PVDF membranes (IPVH00010, Merck Millipore) via a semi- dry cassette (1703940 ...
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bioRxiv - Neuroscience 2020Quote: ... 1:50 Hyaluronan binding protein HABP (#H0161; Merck, Germany) or 1:100 mouse anti-Neurocan (#N0913 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred onto PVDF membranes (0.45µm, Merck Millipore) at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Separated proteins were transferred onto Immobilon-P membrane (Merck) using Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell (Bio-Rad Laboratories) ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by protein precipitation with dichloromethane (Merck, Darmstadt, Germany) and methanol (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2019Quote: ... via a BCA protein assay (Merck-Millipore cat. 71285) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies to basement membrane proteins collagen-IV (Merck-Millipore) or perlecan (Abcam) ...
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bioRxiv - Cell Biology 2020Quote: ... and the protein loading control tubulin (Merck, Sigma-Aldrich). Immuno-reactive complexes were detected using HRP-conjugated secondary antibodies (Cell Signaling Technology ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were electroblotted onto Immobilon-P transfer membranes (Merck). The membranes were blocked with Blocking One for 30 min at room temperature and then incubated with primary antibodies overnight at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... proteins were concentrated using Amicon Ultracel 30K (Merck, UFC503096) according to the manufacturer’s instruction.
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bioRxiv - Molecular Biology 2022Quote: ... and proteins concentrated in Amicon Ultra-15 concentrators (Merck) before flash freezing and storage at - 80 °C.
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bioRxiv - Molecular Biology 2022Quote: Millipore Compartment Protein Extraction Kit (Merck-Millipore, Cat. #2145) was used to deplete cytosolic ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were transferred to a PVDF membrane (Merck Millipore) after which the membrane was blocked using Odyssey Blocking buffer (LI-COR ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced with 10 mM dithioerythritol (Merck-Millipore) for 1 h at 56°C followed by a washing-drying step as described above ...
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bioRxiv - Cancer Biology 2023Quote: Protein lysates were prepared using RIPA buffer (Merck/Millipore). SDS-PAGE and western blotting were performed using standard protocols ...
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bioRxiv - Molecular Biology 2023Quote: Protein concentrations were determined using the Bradford (Merck, B6916) or Lowry assay (DC Protein Assay ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentration was determined via Bradford assay (Merck; #1.10306). Equal amounts of proteins (20–100μg as optimal for each antibody ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µg dephosphorylated myelin basic protein (Merck, 13-110), and purified MAK protein ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto Immobilon-P PVDF membranes (Merck) using a semi-dry blotting system (ATTO) ...
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bioRxiv - Microbiology 2024Quote: ... Protein concentration was measured with BCA assay (Merck Millipore).
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to a PVDF membrane (Merck Millipore) and blocked with 5% skimmed milk-TBST (TBS 10x [Sigma-Aldrich] diluted to 1x in distilled water containing 0.1% Tween 20 [Sigma-Aldrich] ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was concentrated using Amicon filters (Merck, Darmstadt, Germany) in buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... the collagenase inhibitor Ilomastat ((R)-N′-Hydroxy-N-[(S)-2-indol-3-yl-1-(methylcarbamoyl)ethyl]-2-isobutylsuccinamide) (25μM, CC1010, Merck Millipore) or a combination of both ...
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bioRxiv - Bioengineering 2021Quote: ... Part A consisted of 20% hPL (v/v) and 4mg/mL human fibrinogen (Merck, USA) in DMEM/F12 (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... They were coated for 1 hour with 50 μg/mL human plasma fibronectin (Merck Millipore) diluted in PBS and washed three times with PBS.
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bioRxiv - Molecular Biology 2021Quote: ... and goat anti-Human IgG (Fc specific)-Cy3 (1:1000 dilution in binding buffer; Merck). Finally ...
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bioRxiv - Immunology 2021Quote: ... MIP-1β by MILLIPLEX MAP Human High Sensitivity T Cell Panel (Merck Cat# HSTCMAG-28SK) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... Two hundred microliters of medium containing 2 μM human insulin (Merck, Sigma-Aldrich Cat# I9278) or medium only was added to the thoraxes for 10 min (the final concentration of insulin was 1 μM) ...
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bioRxiv - Microbiology 2023Quote: ... and NTK seeded on surfaces coated with 100 μg/ml type IV human collagen (Merck) in defined keratinocyte serum-free medium (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... All human cell lines were cultured at 37℃ in 5% CO2 in DMEM media (Merck) containing 1% antibiotic (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blotted with the following antibodies: anti-human PSEN1-CTF (MAB5643; Merck Millipore); anti-PEN2 (D6G8 ...
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bioRxiv - Immunology 2023Quote: ... cells were cultured in RPMI 1640 (ccpro) supplemented with 10 % heat-inactivated human serum (Merck), 50 U/ml IL-2 (Miltenyi) ...
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bioRxiv - Microbiology 2023Quote: ... Primers for human H19 were used as a positive control for CTCF binding (Merck Millipore).
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bioRxiv - Cell Biology 2024Quote: ... HEK293T cells (ATCC) and FibroGRO Xeno-Free human foreskin fibroblasts (Merck, gift from Martin Lowe) were maintained in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Microbiology 2019Quote: ... It was then filtered again in a vacuum pump (Boeco TM R-300) using 0.45 μm nitrocellulose membranes (Durapore®, Merck™).
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bioRxiv - Microbiology 2021Quote: ... and SsuT3-oE-R (5′-AGTCAG GGATCC CTA CAC CAC CTT CAC TTT GGT ACC) with KOD Hot Start DNA polymerase (Merck Millipore) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... primers 1022700 5F and R were used to amplify the 572 bp 5’ homology flank and primer pair 1022700 3F and R was used to amplify the 673 bp 3’ homology flank (KOD Hot Start DNA Polymerase, Merck Millipore) which were cloned on either side of the sfGFP expression cassette in pkiwi003 (Ashdown et al. ...
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bioRxiv - Biochemistry 2023Quote: ... was used to plot elution profiles of the protein standard mix Supelco® 69385 Protein Standard Mix 15-600 kDa (Sigma-Aldrich/Merck, Darmstadt, Germany), and the analyzed protein samples.
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bioRxiv - Plant Biology 2020Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... After transferring proteins from gels to PVDF membranes (Merck-Millipore) using Trans-Blot Turbo System (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... after which Protein G agarose/Salmon sperm beads (Merck Millipore) were added and incubated for 1 hour ...
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bioRxiv - Biophysics 2020Quote: ... The protein was concentrated using a centrifugal filter (Merck Millipore) and the concentration was determined by UV absorbance using the corresponding ε values (M-1 cm-1).
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bioRxiv - Molecular Biology 2021Quote: Proteins were transferred onto Immobilon-P PVDF membranes (Merck IPVH00010) using a wet-transfer system (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: ... the proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Merck) that were activated in methanol (Carl Roth ...
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bioRxiv - Cell Biology 2022Quote: ... and proteins were transferred to a nitrocellulose membrane (Merck Millipore). The membrane was then incubated with 5% BSA in Tris-buffered saline with tween 20 (Santa Cruz Biotechnology ...
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bioRxiv - Systems Biology 2020Quote: ... proteins were transferred to Immobilon FL PVDF membrane (Merck-Millipore) using semi-dry transfer (25 V for 30 min.) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to 0.45 μm PVDF membranes (Merck Millipore), and blocked with 5% skim milk in TBS-0.1% Tween20 (TBST ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations were determined with a direct detection spectrometer (Merck), and purified proteins were stored at −80°C ...
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bioRxiv - Microbiology 2021Quote: ... Purified proteins were desalted using a PD-10 column (Merck), concentrated using an Amicon Ultra-4 10 kDa cut-off spin column (Merck) ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-myelin basic protein (MBP) (Polyclonal, Merck, Darmstadt, Germany); mouse anti-neurofilament-H protein (clone NE14 ...