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Citations for Merck :
451 - 500 of 578 citations for Plate Sealing since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Reactions were monitored by thin-layer chromatography on commercially available silica pre-coated aluminium-backed plates (Merck Kieselgel 60 F254). Visualisation was under UV light (254 nm and 366 nm) ...
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bioRxiv - Immunology 2023Quote: ... Cells were transferred to plates, and then washed in 100-200 μL FACS buffer (PBS containing 2% FCS, 2 mM EDTA (Merck) and centrifuged at 400 x g for 5 mins ...
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bioRxiv - Plant Biology 2023Quote: ... Cell debris was removed with centrifugation and 2+2 μl of extracts and a commercial pinosylvin standard (ArboNova) were applied on TLC plates Silica gel 60 F254 (Merck), which were developed with chloroform ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 50 µL chloroform : methanol (2 : 1) and 5 µL were loaded on silica gel 60 F254 plates (Merck). To separate trehalose esters of mycolates (TDM ...
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bioRxiv - Microbiology 2023Quote: Time-lapse microscopy analyses were performed in a liquid medium using a CellASIC ONIX platform and compatible B04A plates (Merck), as described previously (Hołówka et al ...
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bioRxiv - Microbiology 2022Quote: ... The reactions were centrifuged for 5 min at 17000 × g and 20 µl of the supernatant was analyzed by TLC on a HPTLC alumina silica gel 60 plate (Merck) using 2-butanol-pyridine-ammonia-water (39:34:10:26 ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa cells grown on 15 cm plates (∼90% confluent) were treated either with 100 μg/mL cycloheximide (CHX, Merck, C1988) for 15 min or with 50 μg/mL puromycin (Puro ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were taken up in MeOH and spotted onto an activated thin layer chromatography (TLC) silica gel 60 plate (Merck). The plate was developed in CHCl3:MeOH:Acetic Acid:H20 (25:15:4:2 ...
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bioRxiv - Microbiology 2023Quote: ... these cultures were diluted 100x in the appropriate medium and either spotted on a sealed agarose pad (MOPS medium, 2% agarose, Figure 1D-F & 4A) or loaded in a CellASIC Onix plate (Merck) and perfused with MOPS medium containing 0.4% arabinose and 20 μg/ml chloramphenicol at 34.5 psi (Figure 3A & 5C) ...
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bioRxiv - Biochemistry 2023Quote: Cancer cells were seeded at a density of 1,000 cells/ 100 μL complete medium into 96-well cell culture plates (#655180, Greiner bio-one, Merck KGaA). After 24 h ...
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bioRxiv - Bioengineering 2023Quote: ... The pre-adipocytes were plated in custom-made plates (Supp. Fig. 15b) and cultured in growth medium -low glucose (1g l-1) DMEM (Merck) supplemented with 10% FBS (Merck) ...
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bioRxiv - Microbiology 2023Quote: ... The whole sample was applied to a high-performance thin-layer chromatography (HPTLC) silica gel 60 plate (10 x 10 cm; Merck) with a Hamilton syringe ...
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatant was added to Fe-NTA agarose beads (PureCube) on a multiscreenHTS-HV 0.45 μm 96 well filter plate (Merck Millipore). After loading ...
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bioRxiv - Microbiology 2023Quote: R47 and R32 wild type strains were inoculated and grown in 6-well plates as described in the RNA extraction section except that 1 ml 0.38 M NaOH (Merck, Germany) was added to the middle two wells to capture HCN in the surrounding ...
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bioRxiv - Microbiology 2023Quote: ... Lipid extracts were then applied to silica gel high-performance thin-layer chromatography (HPTLC) plates (silica gel 60 F254, Merck) using a Linomat 5 sample application unit (CAMAG) ...
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bioRxiv - Bioengineering 2023Quote: ... The hydrolytic products were separated by thin layer chromatography (TLC) on Partisil K6 Silica gel 60 plates (250 µm thickness, 20 × 20 cm; Merck) with developing solvent ...
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bioRxiv - Cell Biology 2023Quote: ... we seeded cells at 2 * 106 cells/ mL in S2 media in 2 mL media/ well in a 6-well plate with either DMSO (D2650, Merck), 2.5 μM CytD (C2618 ...
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bioRxiv - Biochemistry 2023Quote: ... The reactions were stopped by separating the enzyme and soluble products from the insoluble chitin substrate by filtration using 96-well filter plates (Merck). The concentration of oxidized LPMO products was also determined in aliquots taken from the pre-incubation reaction with β-chitin to allow accounting for carry-over of products potentially formed in the pre-incubation phase.
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bioRxiv - Biochemistry 2023Quote: ... The residue was suspended in 10 µL of n-butanol saturated in water and chromatographed on silica 60 plates (20×20 cm, Merck). Plates were developed for 7-10 cm with isopropyl alcohol/ammonia (32%)/water (6:3:1 by volume) ...
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bioRxiv - Cell Biology 2023Quote: HPAECs were seeded at the density of 10,000 cells/well in a pre-prepared 96-well plate containing 30μl of growth-factor reduced Matrigel (Merck, # CLS354230-1EA) per well (38) ...
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bioRxiv - Biochemistry 2023Quote: ... equivalent masses of total lipid fraction from each strain were spotted onto a high-performance TLC (HPTLC) silica gel 60 plate (Merck) with a Camag ATS4 apparatus ...
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bioRxiv - Immunology 2023Quote: ... difficile cultures were grown in TY broth and sub-cultured to an Optical Density at 600 nm (OD600nm) of 0.05 in 200 μL of BHISG in 96-well flat bottom plates (Merck, #Z707902). Bacterial growth was followed for 24h or 18h with OD600nm measurements every 30 min using GloMax Plate Reader (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... Conidia from 14-day-old fungal cultures grown on culture media agar plates were filtered through two layers of Miracloth (Merck) and incubated with Hoechst 33342 solution (20 mM ...
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bioRxiv - Cancer Biology 2023Quote: Tumorspheres formation assay was performed by seeding transfected cells in Corning Costar ultra-low attachment plates (#CLS3473, Merck, Madrid, Spain) in DMEM F-12 medium (#11320033 ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were centrifuged at 14,000 rpm at room temperature for 5 minutes or filtered in multiscreenHTS-HV 0.45 µm 96 well filter plates with PVDF membranes (Merck Millipore) to remove cell debris and nucleic acid aggregates ...
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bioRxiv - Molecular Biology 2024Quote: ... the plates were incubated for 1 h at 25°C with peroxidase-conjugated donkey anti-chicken IgY (Merck, NJ, USA) diluted 5000 times with HEPES-EDTA containing 1% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... These cells were then transferred to cell-culture plastic plates that had been previously treated with poly-L-lysine 10 μg/ml (Sigma-Merck) to enhance cellular adhesion and promote monolayer cultures ...
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bioRxiv - Cell Biology 2024Quote: ... Experiments for Fig.4 and Extended Data Fig.4 were done using CellASIC ONIX V1 microfluidics platform using Y04C microfluidics plates (Merck), at 32° in YE4S ...
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Gliflozins, sucrose and flavonoids are allosteric activators of lecithin:cholesterol acyltransferasebioRxiv - Biochemistry 2024Quote: ... grown in semi-suspension in 150 mm plates in a mixture of serum free media (ACF CHO medium EX-CELL Merck, CHO ONE media Capricorn Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Animals that were collected on day 2 of adulthood were transferred to plates containing 5-FU (10 µM) (Merck, #F6627) at the L4 larval stage to prevent progeny production ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then plated at a density of 500 cells/cm2 into 6-well plates coated with 1.2% poly(2-hydroxyethylmethacrylate) (Merck, Germany) in 95% ethanol (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... were freshly isolated from the plates by scrapping the agar using water supplied with 0.002% Tween 20 added and then filtered through miracloth (Merck Millipore), centrifuged at 3.000 x g for 7 min ...
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bioRxiv - Cell Biology 2020Quote: ... the samples containing 20 nmol phosphate were subjected to TLC on an HPTLC plate (Silica gel 60; Merck Millipore, MA, USA) in the solvent system hexane/diethyl ether/acetic acid (80:20:1 ...
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bioRxiv - Microbiology 2020Quote: A 5-10 µl aliquot of the filtered Aqueous Garlic Extract (AGE) was applied 1 cm away from the base of silica gel 60 TLC plate (Merck Millipore). The applied sample was air dried for 10 min at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... We dissolved dried lipid extracts in 40 μL of 1:1 methanol:CHCl3 and used 5-10 μL of sample for thin layer chromatography (TLC) on TLC silica gel 60 plates (Merck, Germany) with a mixture of 65:25:10 CHCl3:CH3OH:CH3COOH for the mobile phase ...
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bioRxiv - Biochemistry 2019Quote: ... An aliquot of 10 μL of the reaction mixtures were spotted on HPTLC (High Performance TLC) Silica Gel 60 Glass Plates (Merck KGaA), and the plates were developed with a phenol:n-butanol:formate:water (12:3:2:3 v/v ...
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bioRxiv - Plant Biology 2021Quote: ... concentrated lipid extracts with a defined chlorophyll a concentration (in a range of 5-10 μg) were applied to HPTLC silica gel 60 plates (Merck, Germany) with the CAMAG Linomat 5 in the form of bands and developed in TLC chambers based on a method described in Ventrella et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the PCR fragment was amplified with the primers detailed in Supplementary Table 1 and purified in MultiScreen TM Vacuum Manifold 96-well plates (Merck Millipore). The purified PCR fragment was incubated with either the enzyme NlaIII or water (negative control ...
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bioRxiv - Neuroscience 2020Quote: ... the beads were suspended in 100 μl Sheath fluid and transferred to the 96 well flat bottom plate to be read on the FlexMap 3D system equipped with xPONENT software (Merck, Germany). The median fluorescence intensity (MFI ...
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bioRxiv - Neuroscience 2021Quote: ... Cell pellet was resuspended in medium and cells were seeded with fresh medium on plates or coverslips (Corning, Merck, Darmstadt, Germany) coated with PLO (0.1 mg/mL in borate buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The lipids were re-dissolved in 200 μl of chloroform and 5 μl sample was loaded onto a Silica TLC plate (Silica gel 60 F254–Merck Millipore) and separated of lipids by solvent system (chloroform ...
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bioRxiv - Biochemistry 2021Quote: ... Dried lipids were resuspended in 150 ml of CHCl3 and spotted on silica gel high-performance–TLC (HPTLC) plates (Merck, Germany) and resolved with a mixture of chloroform ...
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bioRxiv - Molecular Biology 2020Quote: ... the cells were grown in 24-well plates and treated with increasing concentrations of CaCl2 and NaH2PO4 · Na2HPO4 alone or in combination with PPi (Merck, #P8010) and/or phosphatase (Prospec ...
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bioRxiv - Plant Biology 2021Quote: ... lipid extracts were roughly separated into polar and non-polar lipids by thin-layer chromatography (TLC) using silica plates (TLC silica gel 60, Merck Millipore) developed with chloroform because the high amount of shikonin derivatives hampered the chemical analysis of TAG and polar lipids by LC-MS ...
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bioRxiv - Immunology 2020Quote: PBMCs were rested overnight in complete medium and seeded at 2 × 105 cells/well in MultiScreen HTS Filter Plates (Merck Millipore) pre-coated with anti-IFN-γ (clone 1-D1K ...
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bioRxiv - Plant Biology 2021Quote: ... 80 µL of the dissolved samples were spotted with a TLC spotter to TLC plates (TLC Silica gel 60, Merck KGaA). For extraction of TGs ...
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bioRxiv - Microbiology 2020Quote: ... A serial dilution of the homogenized material was plated out onto blood agar (Substrat, Sahlgrenska Universitetssjukhuset, Sweden) and Thiosulfate-citrate-bile salts-sucrose agar plates (86348, Merck, Germany). The number of Vibrio cholerae CFU per intestine was calculated.
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bioRxiv - Microbiology 2021Quote: ... structured microcosms were set-up in multi-well plates with a 0.22 µm filter membrane at the bottom of the well (Merck, Darmstadt, Germany) and containing 250 mg of sterile glass beads (with diameter of 80 to 120 µm ...
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bioRxiv - Microbiology 2020Quote: The crude extract and purified fractions were separated on TLC Silica gel 60 F254 plates (Merck Life Science Pvt. Ltd., Germany) using the solvent system of hexane ...
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bioRxiv - Microbiology 2019Quote: ... Dried samples were resuspended in 100 µL of hexane and aliquots were transferred on the TLC Silica gel 60 plates (Merck, Germany). Plates were resolved for approximately 30 minutes in hexane ...