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Citations for Merck :
451 - 500 of 616 citations for PCR Plates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The mix was transferred to a plate coated with avidin and the FLAG-peptide was detected with an anti-FLAG-alkaline phosphatase conjugate (Merck), developed with pNPP SigmaFAST (Merck ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was isolated from 2-week-old seedlings grown on plates under 16-hour photoperiod using RNAzol reagent (Sigma-Aldricht, Merck) according to the manufacturer’s instructions and DNA precipitation with a 4-fold volume of bromoanisole ...
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bioRxiv - Plant Biology 2022Quote: ... Five μl of each reaction mixture were loaded onto a TLC plate (TLC Silica gel 60 F254, Merck, Darmstadt, DE). The mobile phase was 7 vol-parts of 1-propanol mixed with 8 vol-parts of H2O ...
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bioRxiv - Plant Biology 2022Quote: ... The purity of compounds was checked using 1H NMR or thin layer chromatography (TLC) using pre-coated aluminium-backed plates (silica gel 60 F254, Merck) and compounds were visualised by UV radiation at 254 nm and then using an anisaldehyde spray reagent (1% p-anisaldehyde:2% H2SO4 ...
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bioRxiv - Immunology 2023Quote: ... Cells were transferred to plates, and then washed in 100-200 μL FACS buffer (PBS containing 2% FCS, 2 mM EDTA (Merck) and centrifuged at 400 x g for 5 mins ...
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bioRxiv - Plant Biology 2023Quote: ... Cell debris was removed with centrifugation and 2+2 μl of extracts and a commercial pinosylvin standard (ArboNova) were applied on TLC plates Silica gel 60 F254 (Merck), which were developed with chloroform ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 50 µL chloroform : methanol (2 : 1) and 5 µL were loaded on silica gel 60 F254 plates (Merck). To separate trehalose esters of mycolates (TDM ...
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bioRxiv - Microbiology 2023Quote: Time-lapse microscopy analyses were performed in a liquid medium using a CellASIC ONIX platform and compatible B04A plates (Merck), as described previously (Hołówka et al ...
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bioRxiv - Microbiology 2022Quote: ... The reactions were centrifuged for 5 min at 17000 × g and 20 µl of the supernatant was analyzed by TLC on a HPTLC alumina silica gel 60 plate (Merck) using 2-butanol-pyridine-ammonia-water (39:34:10:26 ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa cells grown on 15 cm plates (∼90% confluent) were treated either with 100 μg/mL cycloheximide (CHX, Merck, C1988) for 15 min or with 50 μg/mL puromycin (Puro ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were taken up in MeOH and spotted onto an activated thin layer chromatography (TLC) silica gel 60 plate (Merck). The plate was developed in CHCl3:MeOH:Acetic Acid:H20 (25:15:4:2 ...
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bioRxiv - Microbiology 2023Quote: ... these cultures were diluted 100x in the appropriate medium and either spotted on a sealed agarose pad (MOPS medium, 2% agarose, Figure 1D-F & 4A) or loaded in a CellASIC Onix plate (Merck) and perfused with MOPS medium containing 0.4% arabinose and 20 μg/ml chloramphenicol at 34.5 psi (Figure 3A & 5C) ...
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bioRxiv - Biochemistry 2023Quote: Cancer cells were seeded at a density of 1,000 cells/ 100 μL complete medium into 96-well cell culture plates (#655180, Greiner bio-one, Merck KGaA). After 24 h ...
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bioRxiv - Bioengineering 2023Quote: ... The pre-adipocytes were plated in custom-made plates (Supp. Fig. 15b) and cultured in growth medium -low glucose (1g l-1) DMEM (Merck) supplemented with 10% FBS (Merck) ...
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bioRxiv - Microbiology 2023Quote: ... The whole sample was applied to a high-performance thin-layer chromatography (HPTLC) silica gel 60 plate (10 x 10 cm; Merck) with a Hamilton syringe ...
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatant was added to Fe-NTA agarose beads (PureCube) on a multiscreenHTS-HV 0.45 μm 96 well filter plate (Merck Millipore). After loading ...
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bioRxiv - Microbiology 2023Quote: R47 and R32 wild type strains were inoculated and grown in 6-well plates as described in the RNA extraction section except that 1 ml 0.38 M NaOH (Merck, Germany) was added to the middle two wells to capture HCN in the surrounding ...
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bioRxiv - Microbiology 2023Quote: ... Lipid extracts were then applied to silica gel high-performance thin-layer chromatography (HPTLC) plates (silica gel 60 F254, Merck) using a Linomat 5 sample application unit (CAMAG) ...
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bioRxiv - Bioengineering 2023Quote: ... The hydrolytic products were separated by thin layer chromatography (TLC) on Partisil K6 Silica gel 60 plates (250 µm thickness, 20 × 20 cm; Merck) with developing solvent ...
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bioRxiv - Cell Biology 2023Quote: ... we seeded cells at 2 * 106 cells/ mL in S2 media in 2 mL media/ well in a 6-well plate with either DMSO (D2650, Merck), 2.5 μM CytD (C2618 ...
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bioRxiv - Biochemistry 2023Quote: ... The reactions were stopped by separating the enzyme and soluble products from the insoluble chitin substrate by filtration using 96-well filter plates (Merck). The concentration of oxidized LPMO products was also determined in aliquots taken from the pre-incubation reaction with β-chitin to allow accounting for carry-over of products potentially formed in the pre-incubation phase.
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bioRxiv - Biochemistry 2023Quote: ... The residue was suspended in 10 µL of n-butanol saturated in water and chromatographed on silica 60 plates (20×20 cm, Merck). Plates were developed for 7-10 cm with isopropyl alcohol/ammonia (32%)/water (6:3:1 by volume) ...
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bioRxiv - Cell Biology 2023Quote: HPAECs were seeded at the density of 10,000 cells/well in a pre-prepared 96-well plate containing 30μl of growth-factor reduced Matrigel (Merck, # CLS354230-1EA) per well (38) ...
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bioRxiv - Biochemistry 2023Quote: ... equivalent masses of total lipid fraction from each strain were spotted onto a high-performance TLC (HPTLC) silica gel 60 plate (Merck) with a Camag ATS4 apparatus ...
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bioRxiv - Immunology 2023Quote: ... difficile cultures were grown in TY broth and sub-cultured to an Optical Density at 600 nm (OD600nm) of 0.05 in 200 μL of BHISG in 96-well flat bottom plates (Merck, #Z707902). Bacterial growth was followed for 24h or 18h with OD600nm measurements every 30 min using GloMax Plate Reader (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... Conidia from 14-day-old fungal cultures grown on culture media agar plates were filtered through two layers of Miracloth (Merck) and incubated with Hoechst 33342 solution (20 mM ...
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bioRxiv - Cancer Biology 2023Quote: Tumorspheres formation assay was performed by seeding transfected cells in Corning Costar ultra-low attachment plates (#CLS3473, Merck, Madrid, Spain) in DMEM F-12 medium (#11320033 ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were centrifuged at 14,000 rpm at room temperature for 5 minutes or filtered in multiscreenHTS-HV 0.45 µm 96 well filter plates with PVDF membranes (Merck Millipore) to remove cell debris and nucleic acid aggregates ...
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bioRxiv - Cell Biology 2023Quote: For the thin layer chromatography (TCL) an aliquot of lipid extract corresponding to 8 mg of DCW was applied to silica gel TLC plates (Merck) by a Linomat 5 semiautomatic sample applicator (Camag) ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were monitored by thin-layer chromatography on commercially available silica pre-coated aluminium-backed plates (Merck Kieselgel 60 F254). Visualisation was under UV light (254 nm and 366 nm) ...
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bioRxiv - Microbiology 2024Quote: Extracted lipid (dried organic mixture) was solubilized in chloroform and 4µg was resolved by 2D TLC on aluminum-backed silica gel plates (Merck, India) by spotting the extract using microcapillary pipettes as described Pirson et al. ...
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bioRxiv - Cell Biology 2024Quote: Samples were resuspended in 6 μL 40% (v/v) 1-propanol with vortexing and bath sonication were applied to Silica Gel 60 aluminium-backed HPTLC plates (MERCK) (30 ...
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bioRxiv - Microbiology 2023Quote: Polar lipids were separated by 2D-TLC by spotting 200 μg of total lipids on 60 F254 silica gel plates (Merck) using chloroform ...
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bioRxiv - Neuroscience 2024Quote: ... Neurons were collected in Eppendorf tubes by disrupting adhesion to the plate with a jet of medium and washed twice in ice-cold PBS with Ca2+ and Mg2+ (Merck), supplemented with complete ...
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bioRxiv - Immunology 2024Quote: 1×106 of splenocytes or lymph node cells or primary B cells were added to 24-well plates and were stimulated with the TLR4 agonist LPS (L6529, Merck), the TLR7 agonist Imiquimod (HY-B0180 ...
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Insight into the regulatory mechanism of the MFS transporter, SCO4121 by the MarR regulator, SCO4122bioRxiv - Molecular Biology 2024Quote: ... the MSA agar plate was flooded and 500 µg/ml of nalidixic acid (Himedia) and 1 mg/ml of apramycin (Merck). After incubation ...
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bioRxiv - Immunology 2024Quote: ... Neutralized bovine collagen I (2 mg/ml) was plated 40 µl per well in a black 96-well plate with flat clear bottom (Corning/Merck). The plate was kept at 37°C with 5 % CO2 for 1 hour ...
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bioRxiv - Immunology 2024Quote: ... K562-pCasper target cells were resuspend in neutralized bovine collagen I (2 mg/ml) and plated in a black 96-well plate with flat clear bottom (Corning/Merck) at a density of 25,000 cells/40 µl per well ...
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bioRxiv - Molecular Biology 2019Quote: ... an EcoRI restriction site was removed by performing a PCR on AT1.03 and AT1.03R122KR126K pDRF-GW using KOD polymerase (Merck-Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... The PCR products of control and sort2 were concentrated by using Amicon Ultra-0.5 10K (UFC501096, Merck Millipore), and analyzed by pair-end sequencing using NovaSeq 6000 system (Illumina) ...
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bioRxiv - Cell Biology 2020Quote: ... the samples containing 20 nmol phosphate were subjected to TLC on an HPTLC plate (Silica gel 60; Merck Millipore, MA, USA) in the solvent system hexane/diethyl ether/acetic acid (80:20:1 ...
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bioRxiv - Microbiology 2020Quote: A 5-10 µl aliquot of the filtered Aqueous Garlic Extract (AGE) was applied 1 cm away from the base of silica gel 60 TLC plate (Merck Millipore). The applied sample was air dried for 10 min at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... We dissolved dried lipid extracts in 40 μL of 1:1 methanol:CHCl3 and used 5-10 μL of sample for thin layer chromatography (TLC) on TLC silica gel 60 plates (Merck, Germany) with a mixture of 65:25:10 CHCl3:CH3OH:CH3COOH for the mobile phase ...
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bioRxiv - Biochemistry 2019Quote: ... An aliquot of 10 μL of the reaction mixtures were spotted on HPTLC (High Performance TLC) Silica Gel 60 Glass Plates (Merck KGaA), and the plates were developed with a phenol:n-butanol:formate:water (12:3:2:3 v/v ...
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bioRxiv - Plant Biology 2021Quote: ... concentrated lipid extracts with a defined chlorophyll a concentration (in a range of 5-10 μg) were applied to HPTLC silica gel 60 plates (Merck, Germany) with the CAMAG Linomat 5 in the form of bands and developed in TLC chambers based on a method described in Ventrella et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the PCR fragment was amplified with the primers detailed in Supplementary Table 1 and purified in MultiScreen TM Vacuum Manifold 96-well plates (Merck Millipore). The purified PCR fragment was incubated with either the enzyme NlaIII or water (negative control ...
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bioRxiv - Neuroscience 2020Quote: ... the beads were suspended in 100 μl Sheath fluid and transferred to the 96 well flat bottom plate to be read on the FlexMap 3D system equipped with xPONENT software (Merck, Germany). The median fluorescence intensity (MFI ...
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bioRxiv - Neuroscience 2021Quote: ... Cell pellet was resuspended in medium and cells were seeded with fresh medium on plates or coverslips (Corning, Merck, Darmstadt, Germany) coated with PLO (0.1 mg/mL in borate buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The lipids were re-dissolved in 200 μl of chloroform and 5 μl sample was loaded onto a Silica TLC plate (Silica gel 60 F254–Merck Millipore) and separated of lipids by solvent system (chloroform ...
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bioRxiv - Biochemistry 2021Quote: ... Dried lipids were resuspended in 150 ml of CHCl3 and spotted on silica gel high-performance–TLC (HPTLC) plates (Merck, Germany) and resolved with a mixture of chloroform ...