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Citations for Merck :
451 - 500 of 3182 citations for L Isoleucine N Fmoc 1 13C 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: Phosphorylation of Sam68 N-term and C-term were obtained by incubation with CDK1/Cyclin B (Merck, catalogue 14-450) at a Cdk1:Sam68 ratio of 5:1000 at 20 °C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The frozen cell pellets were incubated with 25 mM Tris buffer (pH 7.4) containing 0.05% n-dodecyl-β-D-maltoside (Merck, D4641) and 1 mM CaCl2 ...
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bioRxiv - Biochemistry 2021Quote: ... were constructed using standard genetic engineering techniques with a His6 tag at the N-terminus using the pET28a vector (Novagen, Merck). The recombinant CAHS1 protein ...
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bioRxiv - Biochemistry 2021Quote: ... varieornatus CAHS1 protein was constructed and cloned as a fusion protein with a hexahistidine (His6) tag at the N-terminus using a pET28a vector (Novagen, Merck). CAHS1-N (Met1-Ser96 ...
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bioRxiv - Cell Biology 2019Quote: Pooled extracts of additional control pancreas (n=6) and plasma (n=6) of the experimental groups were filtered with a 30 kDa cut-off Microcon filter (Merck Millipore ...
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bioRxiv - Microbiology 2019Quote: ... then the procedure was repeated under anaerobic conditions (approx. 0.35% oxygen) with 20 mM Trimethylamine N-oxide dehydrate (TMAO) (Merck, 92277) as a terminal electron acceptor ...
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bioRxiv - Biochemistry 2021Quote: ... Reactions were stopped at each time point using 4 N formic acid and analyzed by PEI-Cellulose-F TLC (Merck) developed in 0.4 M potassium phosphate buffer (pH 3.4) ...
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bioRxiv - Immunology 2021Quote: ... neuraminidase-specific inhibitor DANA was added to a final concentration of 100 μM (N-Acetyl-2,3-dehydro-2-deoxyneuraminic acid, Merck, USA). It was used for 48 h prior stimulus.
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bioRxiv - Plant Biology 2023Quote: ... the sequence of CBL1 was fused with an N-terminal 2xStrepII tag in the pET-24b vector (Merck, Darmstadt, Germany). Sequences of active CPK5 and CIPK26 were fused with an N-terminal 2xStrepII tag in the pIVEX-1.3WG vector (Biotechrabbit ...
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bioRxiv - Biochemistry 2023Quote: ... The residue was suspended in 10 µL of n-butanol saturated in water and chromatographed on silica 60 plates (20×20 cm, Merck). Plates were developed for 7-10 cm with isopropyl alcohol/ammonia (32%)/water (6:3:1 by volume) ...
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bioRxiv - Cell Biology 2023Quote: ... WM983B cells were infected by lentivirus in the presence of 5µg/mL polybrene (ref. n°TR-1003, Merck/Sigma-Aldrich) followed by antibiotic selection (puromycin 1µg/mL ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The gelatinous sack surrounding the eggs was removed using 4% L-Cysteine (Merck Milipore, USA) and followed by microinjecting the zygotes with Morpholino antisense oligonucleotide (MO) ...
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bioRxiv - Biochemistry 2022Quote: ... and the cells grown in 2 L of Overnight Express Instant LB medium (Merck, Germany) containing 100 μg/ml ampicillin at 28 °C for approximately 32 h ...
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bioRxiv - Microbiology 2022Quote: ... diluted in RPMI 1640 medium supplemented 2 g/L of NaHCO3 (Merck®, Burlington, MA) and 10% fetal bovine serum (Gibco® ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The gelatinous egg sack was removed using 3% L-Cysteine (Merck Millipore, Burlington, MA, USA) and followed by microinjection of viral mimics or shRNAs.
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bioRxiv - Cell Biology 2022Quote: ... PBMC proliferation was stimulated with phytohemagglutinin-L (PHA, 4.8 µg/ml (Biochrom, Merck Millipore, M5030)) ...
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bioRxiv - Physiology 2022Quote: ... Mild anesthetic induction by MS-222 (50 mg/L, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) [74] was used to treat five masu salmon and two cherry salmon until there was minimal gill movement ...
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bioRxiv - Microbiology 2022Quote: ... Selenite solutions were prepared from a 1,000 mg/L standard solution (Tritrisol-Merck, Darmstadt, Germany). Appropriate dilutions were made to prepare the standards ...
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bioRxiv - Immunology 2022Quote: DEREG and C57B6/L mice were injected with 200 ng of diphtheria toxin (DT, Merck) one day before adoptive T cell transfer ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µM trans- Epoxysuccinyl-L-leucylamido(4-guanidino)butan (E64, Sigma-Aldrich, Merck, Taufkirchen, Germany) was added ...
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bioRxiv - Neuroscience 2023Quote: ... plated onto 60mm dishes pre-coated with poly-L-ornithine (10ug/ml; #P4957-50ML, Merck), and stored in a 37°C incubator with 5%CO2.
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bioRxiv - Evolutionary Biology 2022Quote: ... The gelatinous sack surrounding the eggs was removed using 4% L-Cysteine (Merck Milipore, USA) and followed by microinjecting the zygotes with antisense MOs ...
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bioRxiv - Neuroscience 2023Quote: ... and osmolarity was at 310–320 mOsm/L (all reagents provided by Merck/Sigma-Aldrich). Patch pipettes were pulled from borosilicate glass (3–10 M ...
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bioRxiv - Molecular Biology 2024Quote: ... Then the cells were plated on 15% Poly-L-Ornithine (Merck/Sigma A-004-C) and 1:100 Laminin-(Biotechne 3400-010-02 ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
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bioRxiv - Genetics 2023Quote: Chemical extractions of single wasps were performed by immersing them in 50 µl HPLC-grade n-hexane (Merck, KGaA, Darmstadt, Germany) in 2 ml glass vials (Agilent Technologies ...
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bioRxiv - Biochemistry 2023Quote: The SUN domain sequence of human SUN1 (amino-acids 616-812) was fused to a TEV-cleavable N-terminal His6-GCN4 tag and cloned into a pRSF-Duet1 vector (Merck Millipore). The KASH domain sequence of human JAW1/LRMP (amino-acids 515-555 and 531-555 for KASH6 and KASH6 core ...
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bioRxiv - Biochemistry 2023Quote: ... Purified Mpro with authentic N- and C-termini was concentrated by a 10 kDa MWCO Amicon Ultra 50 centrifugal filters (Merck Millipore) at 4 °C to ∼ 10 mg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... IL-10 and TNF-α were quantified in the FN of NL and at 21dpi aged GFAP-IL6Tg and WT mice (n= 4-6/experimental group) using a Milliplex MAP Mouse High Sensitivity kit (#MHSTCMAG-70K; Merck Millipore), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... a cassette coding for tdTomato with an N-terminal nuclear localization sequence and lack of a stop codon and a puromycin cassette with an N-terminal self-cleaving T2A peptide were amplified by KOD polymerase (Merck, 71085) and subjected to Gibson assembly.
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bioRxiv - Neuroscience 2020Quote: ... previously coated with poly-L-ornithine (PLO, 0.01 mg/ml in PBS, Sigma-Aldrich, Merck, Switzerland) for 4 h ...
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bioRxiv - Cell Biology 2020Quote: Primary fibroblast was seeded on coverslips covered with poli-L Lysine (Merck, Darmstadt, Germany, Ref. P1274) and grown at 70% of confluence ...
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bioRxiv - Plant Biology 2021Quote: ... The mQ was then substituted by a solution of L-012 (Merck SML2236, final 180 μM) and horseradish peroxidase (Merck ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 3-(3,5-dioxo-1,2,4-oxadiazolidin-2-yl)-L-alanine (quisqualate) was purchased from Merck (Darmstadt, Germany). AF647-conjugated 9E10 antibody was prepared in-house as described previously (Cook et al. ...
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bioRxiv - Biophysics 2023Quote: ... All glass surfaces were pre-coated with poly-L-lysine (0.1 mg/mL; Merck, Dramstadt, Germany). Transfected cells were carefully washed with PBS several times and fixed with 4% paraformaldehyde solution in PBS for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: Tissue culture plates were incubated with sterile water containing 0.002% Poly-L-Ornithine Solution (PLO) (Merck) overnight ...
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bioRxiv - Microbiology 2023Quote: ... or RPMI-1640 (10.4 g/L RPMI-1640 powder without phenol red (Merck KGaA, Darmstadt, Germany), 1.8% D-Glucose (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... It was inserted with N and C-terminal tags (His6 and StrepII, respectively) in the baculovirus transfer vector pIEX/Bac3 (Merck Novagen #717263).
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bioRxiv - Plant Biology 2023Quote: ... Pulldowns were done as described17 with the following modifications: The biotinylated Aux/IAA7 DII peptide (AKAQVVGWPPVRNYRKN, N-terminal biotin) was bound to streptavidin-agarose beads (Merck, #16-126). 250 μl of total protein extract was combined with 45 μl of DII-beads and either 0 μM or 10 μM cvxIAA or 10 μM IAA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: CC strips were dissected and then transferred to microtiter plate wells containing 5 μM bis-N-methylacridinium nitrate (Lucigenin; Merck, Darmstadt, Germany), some strips were stimulated with NADPH (100 μM ...
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bioRxiv - Cell Biology 2020Quote: Sapphire discs were coated with carbon land markings and incubated with 1x poly-L-lysine (P8920, Merck) in H2O over night at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... were plasma cleaned (pdc-002 plasma cleaner, Harrick) and coated with 1x poly-L-lysine (P8920, Merck) in H2O for 10min ...
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bioRxiv - Neuroscience 2020Quote: The anionic liposome (AL) solution was freshly made of 5.5mg/mL L-α-Lecithin (Merck Millipore, 524617) and cholesterol 1.18mg/mL (Merck Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were maintained in 293T medium composed of DMEM high glucose (4.5 g/l; Merck, NJ) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... 18 mm) were coated with 0.01% poly-l-ornithine solution (A-004-C, Merck-Millipore, Massachusetts, USA) in the dark ...
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bioRxiv - Microbiology 2022Quote: ... The bioreactor was fed with synthetic medium containing 0.05-0.4 g/L APAP (Merck, ≥99.0%, Darmstadt, Germany) as sole carbon source ...
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bioRxiv - Microbiology 2020Quote: ... divergens in vitro cultures were stuck to microscope cover glasses (12 mm) using poly-L-lysine (Merck). Briefly ...
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bioRxiv - Developmental Biology 2022Quote: ... by submersion in 160 mg/l MS-222 (ethyl 3-aminobenzoate methanesulfonate; Sigma-Aldrich, Merck, Darmstadt, Germany) dissolved in tank water ...
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bioRxiv - Biophysics 2023Quote: ... ATCC repository) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) with 4.5 g/l D-glucose (Merck), supplemented with 10% fetal bovine serum and 1% Penicillin-Streptomycin (10,000 U/ml) ...