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Citations for Merck :
451 - 500 of 1975 citations for Goat Anti Human IgM HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was washed 4 times for 5 min with 1 x PBS/Tween20 0.1 % (v/v) and the HRP conjugated secondary antibodies (A5420 and A4416, Merck) were added 1:5000 (goat ...
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bioRxiv - Neuroscience 2024Quote: ... Membrane was washed 3x5 minutes with 50mls PBS and HRP activity was detected using ECL according to the manufacturer’s instructions (Merck Life Science UK Limited Cat no GERPN3243) ...
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bioRxiv - Cell Biology 2024Quote: ... Blots were washed three times in TBS-T followed by incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck). Following washing in TBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... Detection by enhanced chemiluminescence was performed using the Immobilon Forte HRP Western substrate (ref WBLUF0500, Merck Millipore, Darmstadt, Germany) or SuperSignal West Femto Maximum Sensitivity Substrate (ref 34096 ...
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bioRxiv - Bioengineering 2021Quote: ... Part A consisted of 20% hPL (v/v) and 4mg/mL human fibrinogen (Merck, USA) in DMEM/F12 (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... They were coated for 1 hour with 50 μg/mL human plasma fibronectin (Merck Millipore) diluted in PBS and washed three times with PBS.
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bioRxiv - Immunology 2021Quote: ... MIP-1β by MILLIPLEX MAP Human High Sensitivity T Cell Panel (Merck Cat# HSTCMAG-28SK) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... Two hundred microliters of medium containing 2 μM human insulin (Merck, Sigma-Aldrich Cat# I9278) or medium only was added to the thoraxes for 10 min (the final concentration of insulin was 1 μM) ...
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bioRxiv - Microbiology 2023Quote: ... and NTK seeded on surfaces coated with 100 μg/ml type IV human collagen (Merck) in defined keratinocyte serum-free medium (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... All human cell lines were cultured at 37℃ in 5% CO2 in DMEM media (Merck) containing 1% antibiotic (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... Primers for human H19 were used as a positive control for CTCF binding (Merck Millipore).
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bioRxiv - Immunology 2023Quote: ... cells were cultured in RPMI 1640 (ccpro) supplemented with 10 % heat-inactivated human serum (Merck), 50 U/ml IL-2 (Miltenyi) ...
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bioRxiv - Cell Biology 2024Quote: ... HEK293T cells (ATCC) and FibroGRO Xeno-Free human foreskin fibroblasts (Merck, gift from Martin Lowe) were maintained in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Developmental Biology 2023Quote: ... the slides were incubated with 5 % BSA in TBST supplemented with 10% goat serum (Merck) for 1 h at room temperature (RT) ...
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bioRxiv - Immunology 2021Quote: ... was mixed 1:1 and incubated at RT for 2-3 min or ECL substrate is added (Immobilon crescendo western HRP substrate, WBLUR0100, Merck). Membranes were then exposed to film and developed or visualised by chemiluminescence using the G:BOX Chemi gel doc Imaging System Instrument (Syngene) ...
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bioRxiv - Genetics 2021Quote: ... Blots were washed thrice with TBS-T and visualized on a LAS4000 Fuji imaging system after incubating with Immobilon Western Chemiluminescent HRP substrate (Merck). The following antibodies were used ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were detected with a horseradish peroxidase (HRP)-conjugated secondary antibody (1:3000, #170-6515, Biorad or #12-349, Merck Millipore ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 h at room temperature (RT) and revealed by chemiluminescence using Immobilon Crescendo or Forte Western HRP substrate (Millipore Merck).
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bioRxiv - Microbiology 2020Quote: ... the membranes were incubated with the respective HRP-conjugated secondary antibody in 5% dry milk powder in TBS-T for 1h at room temperature washed three times in TBS-T and imaged on an ECL ChemoCam 3.2 Imager (Intas) using Immobilon Forte Western HRP substrate (Merck Millipore). For pulldown of gH-V5 or gH-V5/ gL-Flag complexes ...
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bioRxiv - Cancer Biology 2022Quote: ... HRP substrate was applied (Lumi-Light Western Blotting Substrate cat#12015200001 from Sigma Aldrich, Immobilon Crescendo cat#WBLUR0020 from Merck Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed 3x with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP substrate (Merck Millipore) using a Gel-Doc XR+ System (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... 2010) that recognizes a common epitope on MASP-1,-3 and MAP-1 followed by HRP-conjugated streptavidin (Merck, RPN1231). The plates were revealed using TMB One as the substrate (Kementec ...
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bioRxiv - Immunology 2021Quote: ... The MILLIPLEX®MAP Human Isotyping Magnetic Bead Panel-Isotyping Multiplex Assay (HGAMMAG-301K-06, Merck) was used to measure IgG1 ...
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bioRxiv - Immunology 2019Quote: ... The MILLIPLEX®MAP Human Isotyping Magnetic Bead Panel-Isotyping Multiplex Assay (HGAMMAG-301K-06, Merck Millipore Corporation ...
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bioRxiv - Cell Biology 2019Quote: ... Complement components in the cellular supernatant were quantified using the MILLIPLEX MAP Human Complement Panel (Merck). The read out of the multiplex assay was performed in a Magpix instrument (Luminex).
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bioRxiv - Genetics 2020Quote: ... which was performed by intramuscular injection with rhFSH (recombinant human follitropin alpha, GONAL-F, Merck Serono) for 8 days ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mg/l hydrocortisone and 1 µg/l and human epidermal growth factor (hEGF) (both Merck). To determine EC sex ...
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bioRxiv - Bioengineering 2023Quote: ... the chips were coated with fibronectin (Human Plasma Fibronectin Purified Protein, Merck, Schiphol-Rijk, The Netherlands) in sterile PBS (100 μg/mL ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Human Caco-2 colorectal adenocarcinoma cells (1.7×105 cells/cm2) were cultured in DMEM medium (Merck) containing 10% FCS at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... medium was further exchanged with FibroGRO™ Complete Media Kit for Culture of Human Fibroblasts (Merck), consisting of a fibroblasts-specific basal media supplemented with P/S (0.5%) ...
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bioRxiv - Cell Biology 2020Quote: ... bound secondary antibodies were detected using either EZ-Chemiluminescence Reagent (Geneflow Ltd) or Luminata™ Forte Western HRP Substrate (Merck Millipore) and a Biospectrum 500 imaging system (UVP ...
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bioRxiv - Plant Biology 2019Quote: ... membranes were washed with TBST five times for 5 min each and visualized using a chemiluminescence detection system (Immobilon Western HRP Substrate, Merck Millipore). All steps were carried out with phosphate-buffered saline (PBS) ...
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bioRxiv - Microbiology 2019Quote: ... The membranes were finally washed again 4 times for 10 min in TBS and revealed with Immobilon Western Blotting Chemoluminescence HRP substrate (Merck Millipore) and Super RX-film (Fujifilm ...
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bioRxiv - Developmental Biology 2020Quote: ... membranes were washed 3 times with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP (Horseradish Peroxidase) Substrate (Merck Millipore) using a Gel-Doc XR+ System (BioRad) ...
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bioRxiv - Cell Biology 2020Quote: ... probed with primary antibodies (see Materials above) then the appropriate HRP-conjugated secondary antibody (see Materials above) and signals revealed by enhanced chemiluminescence (Merck P90720) on a ChemiDoc Touch Imaging System (Bio-Rad) ...
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bioRxiv - Developmental Biology 2022Quote: ... The membranes were washed 3x in TBST for 10 min again and afterwards incubated in Immobilon ECL Ultra Western HRP Substrate (Merck, WBULS0100) for 2-5 min and scanned with GS-800 densitometer (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... the membranes were washed three times with TBST for 10 min and proteins were detected via chemiluminescence using Immobilon® Forte Western HRP substrate (Merck). For the multiplex detection of native LpxC and the LPS level 12% stain-free FastCast Acrylamide gels (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... Blots were then washed with TBS-T three times for five minutes and exposed to Immobilon Western Chemiluminescent HRP substrate (Merck Millipore) according to product instructions for 30 seconds before being developed ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed four times for 5 minutes in PBST and incubated with Immobilon® Forte Western HRP Substrate (Merck, WBLUF0100) for 3 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... The signal was visualized using appropriate primary and secondary antibodies and the Immobilon™ Western Chemiluminescent HRP substrate (Merck, Darmstadt, Germany) and developed on an X-ray film ...
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bioRxiv - Biophysics 2021Quote: ... Immuno-identification of the samples was done using α-c-Myc rabbit antibody and followed by a secondary HRP-conjugated α-rabbit antibody (Merck). α-Histone H3 rabbit antibody was used as housekeeping protein ...
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bioRxiv - Molecular Biology 2022Quote: ... rinsed with PBS and the proteins were visualized with Immobilon Western Chemiluminiscent HRP substrate (Merck/Millipore, used at 1:1:3 (H2O) dilution ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Immunoreactive bands were detected using iBright CL1000 chemiluminator or LAS 3000 (Fujifilm, Tokyo, Japan) with an enhanced chemiluminescent substrate for the detection of horseradish peroxidase (HRP; Merck, Darmstadt, Germany). The intensity of the RAS band was quantified using ImageJ software and normalized to the intensity of the GAPDH band.
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bioRxiv - Neuroscience 2019Quote: Plasma levels of adiponectin and leptin were measured using commercially available kits (Human Leptin Enzyme Immunoassay, Merck, Cat ...
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bioRxiv - Microbiology 2021Quote: ... Human embryonic kidney (293T) cells were maintained in the Dulbecco’s Modified Eagle Medium (D6046, Merck, Darmstadt, Germany) supplemented with 10% fetal bovine serum (FB-1365 ...
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bioRxiv - Bioengineering 2021Quote: The sEVs used to validate the platform were collected from a commercial human serum purchased from Merck Millipore (same lot in all preparations ...
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bioRxiv - Neuroscience 2021Quote: ... Injections of 50 µl NGF at a concentration of 0.8 µM (NGF, human recombinant, Calbiochem, Merck, Germany) dissolved in phosphate buffer saline (PBS ...
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bioRxiv - Neuroscience 2021Quote: ... The concentrations of Aβ40 and Aβ42 were determined in brain lysates using the ELISA kits according to the manufacturer’s instructions (human Aβ40 and Aβ42 brain ELISA, Merck).
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bioRxiv - Genomics 2022Quote: ... females and males were primed with 20 U and 10 U human chorionic gonadotropin (hCG, Pregnyl, Merck), respectively ...