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Citations for Merck :
451 - 500 of 2184 citations for Alpha Cyclodextrin Solution 5% w v since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... After 72 hours of incubation at 33°C 5% CO2 wells were stained with Gram’s crystal violet solution (Merck KGaA, 64271 Damstadt, Germany) plus 5% formaldehyde 40% m/v (Carlo ErbaSpA ...
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bioRxiv - Cancer Biology 2023Quote: ... peptides were prepared by lysis of cells with a 5% SDS solution and Roche cOmplete™ Mini EDTA-free Protease Inhibitor Cocktail (Merck Darmstadt, Germany), followed by sonication (10 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Milli-Q water was used for preparing all aqueous solutions (18 MΩ×cm, <5 ppb, Millipore Milli-Q® Reference A+ system, Merck Millipore, Germany). LC-MS grade acetonitrile (ACN ...
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bioRxiv - Genomics 2022Quote: ... followed by quenching with final 1 x Glycine solution for 5 min at RT utilizing the Magna Nuclear RIP (Cross-Linked) Nuclear RNA-Binding Protein Immunoprecipitation Kit (Merck millipore, 17-10520). Cross-linked cells were then centrifuged at 800 x g at 4°C and washed three times with ice cold DPBS ...
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bioRxiv - Microbiology 2022Quote: ... After 72 hours of incubation at 33°C 5% CO2 wells were stained with Gram’s crystal violet solution (Merck KGaA, 64271 Damstadt, Germany) plus 5% formaldehyde 40% m/v (Carlo ErbaSpA ...
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bioRxiv - Molecular Biology 2024Quote: ... Mayer’s hematoxylin solution (Merck) was used for counterstaining ...
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bioRxiv - Immunology 2023Quote: ... Mayer’s Haematoxylin solution (Merck) was applied for 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Tyrodés solution #T1788 (Merck) was used during a few seconds to dissolve zona pellucida ...
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bioRxiv - Developmental Biology 2024Quote: ... Accumax solution (Merck, A7089), 1 ml for human and 200 μl for mouse ...
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bioRxiv - Microbiology 2021Quote: ... by high-performance thin-layer chromatography (HPTLC) in CHCl3/MeOH 85:15 v/v on glass-backed 10 × 10 cm silica gel 60 F254 plates (Merck) and a small sidebar of the plate was stained with Hanessian’s stain (0.5 g of cerium(IV ...
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bioRxiv - Developmental Biology 2020Quote: ... for 1 h at 50 V followed by 3.5 h at 100 V and blotted on PVDF-membranes (Immobilon-PSQ Membrane, Merck Millipore). Incorporation of [35S]-methionine into newly synthesized proteins enabled the detection of translation products by phosphor imaging (exposure time of 1 day).
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bioRxiv - Evolutionary Biology 2023Quote: The frozen cell pellet was homogenized in RIPA buffer (Fujifilm, Osaka, Japan) containing 1/100 (v/v) in a final volume of Protease Inhibitor Cocktail (Merck) for 30 min at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... for 90 min at 120 V and blotted at constant 30 V for 30 min onto 0.45 µm Immobilon-FL PVDF membrane (Merck Millipore). After the transfer ...
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bioRxiv - Microbiology 2023Quote: ... Hydrophobic surface treatment was performed after bonding by flushing with 1% (v/v) trichloro(1H,1H,2H,2H-perfluorooctyl)silane (Merck) in HFE-7500 and ...
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bioRxiv - Microbiology 2022Quote: ... wells were washed twice with warm PBS before incubation in 500 μL pre- warmed DMEM with 1% (v/v) Nutridoma (Merck) supplemented with 5 μM clickable sphingosine (Cayman Chemical ...
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bioRxiv - Microbiology 2024Quote: ... We stored them at -80 °C in phosphate buffered saline (PBS) with 20 % (v/v) glycerol (anhydrous; Merck, Darmstadt, Germany) and cultivated the isolates on Columbia blood agar plates containing 5 % sheep blood (Mast Diagnostica ...
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bioRxiv - Microbiology 2024Quote: ... Samples were separated on a 1.2 % agarose gel stained with 0.01 % (v/v) GelGreen Nucleid Acid Stain (Merck, Darmstadt, Germany) in TBE buffer for 2 h at 120 V ...
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bioRxiv - Cell Biology 2024Quote: Samples were resuspended in 6 μL 40% (v/v) 1-propanol with vortexing and bath sonication were applied to Silica Gel 60 aluminium-backed HPTLC plates (MERCK) (30 ...
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bioRxiv - Bioengineering 2022Quote: ... the wells were rinsed 3 times with PBS and covered with 100 μL 0.5% (v/v) Triton-X 100 (Merck, 1.08603.1000) in PBS for 5 minutes to permeabilize cells ...
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bioRxiv - Bioengineering 2023Quote: ... the wells were rinsed with PBS twice and covered with 100 μL 0.5% (v/v) Triton-X 100 (Merck, 1.08603.1000) in PBS for 5 minutes to permeabilize cells ...
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bioRxiv - Microbiology 2024Quote: ... complemented with 10 % (v/v) heat-inactivated (56°C at 30 min) FBS and 200 µM BAPTA (Merck Millipore, 196418)] with or without 1.8 mM CaCl2 (Roth ...
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bioRxiv - Microbiology 2024Quote: ... LS-ARS2 overnight culture was inoculated (1% v/v) in MRS broth adjusted to pH 4 and pH 3 with HCl (1N, Merck), followed by incubation for 0 ...
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bioRxiv - Microbiology 2024Quote: ... Antibody cross-linked beads were prepared for use in immunoprecipitation by washing in 500µl of lysis buffer (20mM Tris, 150mM NaCl, 5mM MgCl2, 0.5% [v/v] NP40, 25 unit/ml Benzonase (Merck Millipore), fresh cOmplete protease inhibitor [1 tablet per 10ml of buffer] ...
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bioRxiv - Microbiology 2024Quote: ... The resulting concentrated retentate was then filtered through a 0.22 μm filter and added with stabilizing buffer containing 2% (v/v) gelatin hydrolysate (Merck G0262) and 5% (v/v ...
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bioRxiv - Pathology 2024Quote: ... v/v) and loaded onto a TLC plate (10 or 20 µL, HPTLC silica gel 60, 20 × 10 cm, Merck) using a Camag Linomat 5 sampler (Camag ...
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bioRxiv - Bioengineering 2024Quote: ... with media changed every two days and passaged at a confluency of 80% using 0.05% v/v trypsin-EDTA (Merck T3924) in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... organoid culture medium is removed and replaced by fresh medium containing the fluorescent probes to stain the DNA (Hoechst, 1:3000 v/v) and the tubulin (Biotracker 488 Microtubules, Merck, dilution 1:500 used with Verapamil 1:1000 to contain the probe inside the cells).
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bioRxiv - Neuroscience 2020Quote: ... and 0.1% (wt/v) NaN3 (Merck, Darmstadt, Germany) on a shaker ...
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bioRxiv - Immunology 2020Quote: Following protein digestion a 5% volume of the peptide solution was cleaned up with a reverse phase (C18) ZipTip (Merck Millipore, Burlington, MA, USA). The peptide sample was acidified with the addition of 1 μl 1M hydrochloric acid ...
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bioRxiv - Immunology 2022Quote: ... Tissue samples for RNA-protein extraction were cut into small pieces (< 5 mm) and placed in a microcentrifuge tube containing RNAlater® solution (Merck Ltd, Dorset, UK) and stored at −70°C until further use.
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bioRxiv - Genetics 2023Quote: ... and 5-methyltetrahydrofolate (5-MTHF, Merck, #M0132) were added to NMG media at indicated concentrations ...
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bioRxiv - Biochemistry 2020Quote: ... cells were washed 3x in PBS followed by blocking in 16% (v/v) normal goat serum (Merck Milipore, Burlington, MA, USA) supplemented with 0.1% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... for 90 min at 120 V and blotted at constant 30 V for 30 min onto Immobilon-FL PVDF membrane (Merck Millipore). After the transfer ...
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bioRxiv - Neuroscience 2023Quote: ... Protein G Dynabeads (catalogue # 10003D) were washed with 0.02% (v/v) PBS-Tween 20 (Tween-20: catalogue # P2285, Merck; 0.01M PBS) and incubated with 200µL of primary antibody mix (for antibody details see Supplementary Table 5 ...
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bioRxiv - Plant Biology 2023Quote: Plants were grown on 1/2 MS agar plates containing 0.01% (v/v) dimethyl sulfoxide (mock) or 50 ng mL-1 TM (654380; Merck, Darmstadt, Germany) for 14 days ...
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bioRxiv - Microbiology 2023Quote: ... 10% (v/v) glycerol using an Amicon Ultra-4 centrifugal column with a 10 kDa molecular weight cut-off (Merck-Millipore). This process yielded a protein concentrated to ∼10–20 mg/mL ...
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bioRxiv - Genomics 2022Quote: ... During sampling we set a flow rate of 200 l/minute for a total of 60 minutes with 10 ml 0.05 % (v/v) Triton X-100 (T8787-50ML, Merck Sigma Aldrich) as collection buffer prepared with UltraPure DNase/RNase free ...
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bioRxiv - Immunology 2023Quote: ... A549 cell were incubated with lentiviral supernatants diluted 1:20 (v/v) in culture medium containing 10 µg/ml polybrene (Merck Millipore) and centrifuged at 805xg at 20 °C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting concentrate was applied to a 10 - 50% v/v iodixanol step gradient with 10% increments (OptiPrep; Sigma Aldrich, Merck) enriched with sea salts (Sigma Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... Samples for VFA determination were further fixed by adding 1% v/v of 4 M sulfuric acid (H2SO4, Merck Millipore, Germany). The concentrations of VFAs and sulfate were determined using a 930 Compact IC Flex 1 with a Metrosep A supp 7 −250/4.0 column coupled with an 887 Professional UV/VIS detector (Metrohm ...
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bioRxiv - Microbiology 2024Quote: ... 21068028) complemented with 10 % (v/v) heat-inactivated (56°C at 30 min) FBS and 200 µM BAPTA (Merck Millipore, 196418)] with or without 1.8 mM CaCl2 (Roth ...
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bioRxiv - Developmental Biology 2020Quote: Women were stimulated with highly purified urinary hMG (Menopur®, Ferring, Spain) or follitropin alpha (Gonal®, Merck-Serono, Spain), with daily injections of 150-300 IU (Blazquez et al. ...
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bioRxiv - Microbiology 2020Quote: ... The reaction products were separated on thin layer chromatography Silica gel 60 W (Merck) using the solvent system butanol/ethanol/water (5/4/3 v/v/v ...
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bioRxiv - Bioengineering 2021Quote: ... Secondary antibody solutions were supplemented with 1 µg/mL DAPI solution (MBD0015; Merck KGaA) and 1 µg/mL BODIPY™ 493/503 dye (D3922 ...
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bioRxiv - Microbiology 2020Quote: ... to obtain a final concentration of 80 % acetone (v/v) and pipetted on an Ultrafree-MC PTFE membrane 0.2 µm filter (Merck Millipore, Burlington, MA). The filter was incubated for 2 min at room temperature and was then centrifuged at 5000 x g for 1 min ...
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bioRxiv - Neuroscience 2022Quote: ... The pellets were lysed for 1 h using cell protein extraction reagent (CW BioTech, CW0889, China) with protease inhibitor Cocktail set III (1 %, v/v, Merck, 535140, Germany), followed by centrifugation at 500×g for 10 min and the supernatants were collected ...
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bioRxiv - Plant Biology 2021Quote: ... Extracted peptides were dried in a speed-vac and resuspended in (v/v) 2% acetonitrile/0.2% trifluoroacetic acid (Merck, catalog no. 302031). A total of four biological replicates for each sample type was submitted.
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bioRxiv - Immunology 2022Quote: ... at pH 2.0 with final protein concentration of 1.75 mg/mL (Sphβ preparation) or in 40% ethanol (v/v from ethanol absolute, Merck KGaA, Darmstadt, Germany), 0.25 M NaCl at pH 1.8 with final protein concentration of 5 mg/mL (Sphα preparation) ...
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bioRxiv - Biochemistry 2024Quote: ... All media were supplemented with 10% v/v heat-inactivated (56 °C at 30 min) fetal calf serum (FCS) (Sigma/Merck, Darmstadt, Germany). Growth took place at 37 °C and 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: ... solution containing 50% methanol (Merck) and 14% acetic acid (Merck) ...