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Citations for Merck :
451 - 500 of 4974 citations for 7 hydroxy 1H 1 2 4 triazolo 1 5 a pyrimidin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged for 5 min at 20 000 x g and supernatants were immunoprecipitated for 1 h rotating at 4°C with mouse anti-GFP antibody (1 µg antibody per sample, 11814460001 Merck/Sigma) and 20 µl DynabeadsTM Protein G (10004D ...
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bioRxiv - Molecular Biology 2022Quote: ... The beads were pre-bound with 4-5 micrograms of antibodies (H3K4me3 – Merck 07-473; H3K9me2 Abcam ab1220; H3K27me3 – Acive Motif 39155; H3 – Merck 07-10254; PolII – Abcam ab817) before incubation with the sheared chromatin ...
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bioRxiv - Molecular Biology 2021Quote: ... CTCF antibody (07-729, Merck Millipore; 5 μL per ChIP) was coupled with Protein G beads (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... Chromatin was incubated with 5 μL anti-CTCF (Merck-Millipore), 5 μg anti-H3K27ac (Abcam) ...
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bioRxiv - Genetics 2022Quote: ... or 5 μg rabbit IgG isotype control antibody (Merck-Millipore) for 16 h at 4°C with rotation ...
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bioRxiv - Cell Biology 2019Quote: ... dropped onto glass slides and stained with 5% Giemsa (Merck). Cohesion defects were counted in 25 metaphases per slide on two coded slides per condition.
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bioRxiv - Immunology 2019Quote: ... the tissue was mounted using 5% N-propyl gallate (Merck) in glycerol and imaged using a Zeiss Axio Scope A1 (Zeiss) ...
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bioRxiv - Neuroscience 2021Quote: ... and hydrated for 5 min in 99 % ethanol (100983, Merck), 5 min in 95 % ethanol ...
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bioRxiv - Microbiology 2021Quote: ... Parasite nuclei were stained using 5 µg/ml Hoechst (Merck) and Vectashield (Vector Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... and 10 µM fluorescein (46955, CAS 2321-07-5, Merck). The conductivity of 0% NaCl and 100% NaCl was respectively 5.99 µS/cm and 11.02 mS/cm and increased linearly with the NaCl concentration ...
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bioRxiv - Bioengineering 2022Quote: ... after which the digest was treated with 5% FBS (Merck) to inactivate the enzymes ...
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bioRxiv - Neuroscience 2020Quote: ... and 5% potassium chromate (Merck; compare (Das et al., 2013)) ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 mM CaCl2 (#21115, Sigma, part of Merck, Darmstadt, Germany), 3 mM Mg acetate (#63052 ...
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bioRxiv - Microbiology 2022Quote: ... was equilibrated with 5 mM H2SO4 (Titrisol, Merck, Darmstadt, Germany) in water at 55 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 5 µL of Lysonase Bioprocessing Reagent (Merck Millipore, USA) and sonicated 6 x 2 min on ice until the suspension became transparent ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed adding 300 μL of 5% formaldehyde (Merck) in PBS 1x for 30 min at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Tryptic peptides were successively extracted with 5 % formic acid (Merck)/50 % acetonitrile (Merck) ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 3-nitro-L-tyrosine [5 µM] (Merck, Darmstadt, Germany) as internal standard (ISTD ...
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bioRxiv - Microbiology 2021Quote: ... after prefiltration with 5 μm Durapore membrane filters (Merck KGaA) onboard ...
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bioRxiv - Cell Biology 2022Quote: ... Quercetin (10−5 M, 00200595; Sigma-Aldrich/ Merck group, USA) 36 ...
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bioRxiv - Neuroscience 2022Quote: ... was combined with 90μM sygRNA (5’ - GGATTTGGTAATAGCAG AGGGGG 3’) (Merck) at RT for 15 minutes to form an RNP complex ...
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bioRxiv - Immunology 2023Quote: ... Sections were incubated with PBS DAPI (5 µg/mL) (Merck) for 20 minutes and mounted using ProLong Diamond antifade mounting medium (Invitrogen).
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bioRxiv - Immunology 2023Quote: ... Staphylococcus-Enterotoxin-B (SEB) (5 µg/ml, Merck Sigma-Aldrich), DAPI (Merck Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA oligonucleotide G4A4 (5’-AAAAAAGGGGAAAAGGGGAAAAGGGGAAAAGGGGAAAAAA-3’) was purchased from Merck. CD analysis of 2,5 µM RNA was carried out in the buffer used for G4-pulldown ...
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bioRxiv - Systems Biology 2023Quote: ... The reaction was quenched using the final 5% hydroxylamine (Merck) in triethylammonium hydrogen carbonate buffer (Fluka ...
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bioRxiv - Neuroscience 2024Quote: ... 5% glycerol with cOmplete Mini EDTA-free (Merck, Darmstadt, Germany)) was added and the cells were scraped off with a scraper ...
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bioRxiv - Biophysics 2024Quote: ... cell were treated with 5 µg/mL Mitomycin C (Merck) for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... One mL of virus stock and 9 mL of MEM-E supplemented with 1% pyruvate (Merck KGaA, Darmstadt, Germany) were added to a conical tube with 16 × 106 SK-6 cells and shaken for 30 minutes at 104 rpm and 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... and 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI, #10236276001, Merck) staining ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Staining cryosections with 4’,6-diamidino-2-phenylindole (DAPI, Merck) and active caspase-3 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and phosphatase inhibitor cocktail 2 (1:1000) (Merck Life Sciences U.K. Ltd). Cell debris was removed from lysates via centrifugation (275 × g ...
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bioRxiv - Biochemistry 2020Quote: ... 1 % (v/v) 2-mercaptoethanol) and 25 U/mL Benzonase (Merck #70746).
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bioRxiv - Neuroscience 2021Quote: ... 350 µl of a 1:100 dilution of 2-Mercaptoethanol (Merck, 8057400250), 250 µl insulin (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit polyclonal anti-oligodendrocyte transcription factor 2 (Olig2) (Merck-Millipore, 1:200), rabbit polyclonal anti-ionized calcium-binding adapter 1 (Iba-1 ...
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bioRxiv - Pathology 2023Quote: ... 1 µl of 10x TCEP (Tris(2-carboxyethyl)phosphine hydrochloride (#C4706, Merck) was added to the lid and mixed carefully ...
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bioRxiv - Microbiology 2022Quote: ... we used the same approaches as described above and spotted the 5 μl of bacteria on LB agar plates containing 2% of Skim Milk Powder for microbiology (Sigma-Aldrich/Merck KGaA, Darmstadt, Germany). Plates were incubated at 25°C and monitored for hemolytic and protease activities after 1 ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were fixed for 1 h at room temperature with 4% buffered formalin solution containing 1% crystal violet (Merck, Darmstadt, Germany). Finally ...
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bioRxiv - Neuroscience 2019Quote: ... before probing overnight at 4°C with primary antibodies (1:500 mouse anti-NF200 [N0142; Sigma-Aldrich] and 1:500 rabbit anti-peripherin (AB1530; Merck Millipore) in blocking solution ...
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bioRxiv - Biochemistry 2022Quote: ... Purified ShTnsC was concentrated to 1-2 mg mL−1 using 30,000 kDa molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen.
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bioRxiv - Immunology 2022Quote: ... and spleen were harvested and homogenated in PBS for CFU counting or in isotonic buffer (Tris HCl 50 nM, EDTA 2 mM, PMSF 1 mM [Roche Diagnostics GmbH], Triton X-100 1% [Merck Life Science] ...
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bioRxiv - Biochemistry 2021Quote: ... Purified ShTnsC was concentrated to 1-2 mg mL−1 using 30,000 molecular weight cut-off centrifugal filters (Merck Millipore) and flash-frozen in liquid nitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... actin was let to polymerize in PBS for 30 min at room temperature before adding 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholiniumchloride (DMTMM, MERCK) cross-linker ...
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bioRxiv - Cancer Biology 2022Quote: ... and additional wells with 20% biotinylated PLL-g-PEG and FNIII(7-10) were supplemented with 1 µM staurosporine (Merck Millipore) to serve as a positive control for apoptosis ...
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bioRxiv - Microbiology 2023Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with polyclonal rabbit anti-TipR antibodies (1:400 dilution) (Kirkpatrick and Viollier, 2014) or monoclonal rabbit anti-HA antibodies (1:250 dilution) (Clone 114-2C-7, Merck Millipore). ...
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bioRxiv - Microbiology 2021Quote: ... were diluted 1:500 in 4% bovine serum albumin (BSA) (Sigma-Aldrich, Merck, UK).
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bioRxiv - Microbiology 2022Quote: ... followed by 1h treatment with lysozyme at 37ºC (Merck, 100 mg/ml) and 1h proteinase k at 55 ºC (Promrga ...
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bioRxiv - Cell Biology 2020Quote: ... Subsequently 5 volumes of cell culture medium (MEM (Merck KGA, Germany) containing 10% FCS (Merck KGA ...
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bioRxiv - Physiology 2022Quote: ... The EHBDs were treated with GCDCA 5 or 50mM (G0759 Merck) and ursodeoxycholic acid (UDCA ...
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bioRxiv - Neuroscience 2020Quote: ... Absolute ethanol (CAS Number: 64-17-5) was obtained from Merck KGaA (Darmstadt ...
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bioRxiv - Biochemistry 2021Quote: ... and 5 μg of transfer plasmid per plate using polyethylenimine (Merck) as transfection reagent ...