Labshake search
Citations for Merck :
451 - 500 of 546 citations for 7 Methyluric acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Peptides were dissolved in 2% acetonitrile containing 0.1% trifluoroacetic acid and desalted using C18 ZipTips (Merck Millipore, Germany). Each sample was independently analysed on a Q-Exactive hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... Organic acids (lactate, acetate, pyruvate and succinate) were analyzed using a reversed-phased HPLC (Merck-Hitachi, Darmstadt, Germany) equipped with a diode array detector (L-7450 ...
-
bioRxiv - Cell Biology 2022Quote: ... The corresponding GST fusion protein was added to a final concentration of 15 µg/ml in incubation buffer (10 mM Tris pH 8.0, 150 mM NaCl, 0.1 % Tween-20, 3 % BSA (fatty acid free, Merck)) ...
-
bioRxiv - Molecular Biology 2020Quote: ... and plates were developed in n-hexane/diethylether/acetic acid 70:30:5 (v/v/v) (Merck, Roth) in a developing chamber (CAMAG ...
-
bioRxiv - Biochemistry 2020Quote: ... One and a half μl of protein solutions were mixed on the target plate with 0.5 μl of the 20% 2,5-dihydroxybenzoic acid (Merck) solution in 20% ACN ...
-
bioRxiv - Neuroscience 2020Quote: ... The larval samples were digested overnight at room temperature with concentrated (65%) nitric acid (Suprapur®grade, Merck) followed by 20 minutes at 90°C ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were precipitated in 50% (w/v) trichloroacetic acid (Roth) and washed once with ice-cold acetone (Merck). Protein pellets were resuspended in 1 x sodium dodecyl sulphate (SDS ...
-
bioRxiv - Molecular Biology 2022Quote: ... 100 mg of the total lysates were mixed with 1mL 0.1 mg/mL pepsin in 0.5M acetic acid (Merck) and put at 4°C overnight ...
-
bioRxiv - Plant Biology 2022Quote: ... The separation was carried out using a reversed-phase Eclipse plus C 18 column (1.8 μm, 2.1 × 50 mm) with corresponding solvent A [0.1 % formic acid (FA; Merck, USA) in water] and solvent B [0.1 % FA in acetonitrile (Merck ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were precipitated with 50% (w/v) trichloroacetic acid (Roth) and washed once with ice-cold acetone (Merck). Protein pellets were resuspended in 1 x sodium dodecyl sulphate (SDS ...
-
bioRxiv - Developmental Biology 2023Quote: ... prior to fixation embryonic zona pellucidae were removed in prewarmed (37°C) drops of Acid Tyrode’s solution (Merck) diluted in M2 ...
-
bioRxiv - Immunology 2023Quote: ... Tissue samples (200 mg) were digested in 4 ml of EMSURE ISO nitric acid 65% solution (Merck, #1.00456.2508) using MARS 6 microwave extraction system ...
-
bioRxiv - Cell Biology 2023Quote: ... overnight cultures were grown at 30 °C at 200 rpm in Yeast Nitrogen Base without amino acids (Merck) prepared according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... integrity was inspected on 1% agarose gel in TAE buffer pre-stained with GelRed nucleic acid stain (Merck).
-
bioRxiv - Cell Biology 2023Quote: ... beta-galactosidase solution was prepared containing 20 mM citric acid (pH=6) (ref 1.00244.0500, Merck Millipore, Darmstadt, Germany), 5 mM of potassium hexacyano-ferrate (II ...
-
bioRxiv - Molecular Biology 2021Quote: ... and resuspended in 60 μl PFGE wash buffer containing 1 μl lyticase (17 U/μl 10 mM KPO4 pH 7, 50% glycerol Merck L2524 >2000 U/mg) then incubated for 10 min at 50°C ...
-
bioRxiv - Physiology 2020Quote: ... Cows treated with the 7-day CO-Synch + CIDR protocol (n = 769) received administration of 100 μg gonadorelin acetate (GnRH; Fertagyl, Merck Animal Health, Madison, NJ) and insertion of a 1.38 g intravaginal progesterone releasing insert (CIDR® Zoetis ...
-
bioRxiv - Microbiology 2020Quote: ... 48 and 72 hours) of exposure to simulated solar radiation was performed by using 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) (Sigma Aldrich-Merck KGaA, Darmstadtm Germany) solubilized in ethanol ...
-
bioRxiv - Cell Biology 2023Quote: ... to validate the gene expression the plasmid (vide-supra) was transfected into HEK293 cells using PEI (Sigma-Aldrich/Merck Cat. No. 49553-93-7) following an optimized version of the standard protocol(Rajendra ...
-
bioRxiv - Microbiology 2024Quote: ... 15 µg of total protein in a volume of 47 µl 1x sample buffer was treated with 7 U of benzonase (#70746; Merck Millipore, Burlington, MA, USA) in dilution buffer (20 mM HEPES pH 8.0 ...
-
bioRxiv - Developmental Biology 2020Quote: ... and 0.1% sodium dodecyl sulfate (SDS; AppliChem; Darmstadt, Germany) with an ethylenediaminetetraacetic acid (EDTA)-free protease inhibitor cocktail (Merck), phosphatase inhibitor cocktail sets II (Merck ...
-
bioRxiv - Molecular Biology 2020Quote: ... EmbryoMax KSOM medium with 1/2 amino acids without BSA (Reagent setup; Merck Millipore, cat. no. MR-107-D), BSA powder (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2019Quote: ... IFNβ production was stimulated by transfecting cells with (10 μg/mL) polyinosinic: polycytidylic acid (poly I:C) (Sigma Aldrich, Merck). Cells were lysed 6 hours after poly I:C transfection using 100μL 1X passive lysis buffer (Promega) ...
-
bioRxiv - Bioengineering 2022Quote: ... that was recently established by a disruption of both AAH1 and APT1.18 Yeast strains were grown overnight at 30 °C in a synthetic defined SD medium (6.7g/L Yeast Nitrogen Base without Amino Acids (Difco) and 20g/L glucose (Merck)) containing a specific mixture of amino acids and nucleobases ...
-
bioRxiv - Cell Biology 2022Quote: ... resolubilized in 25 µL of 0.05% trifluoroacetic acid in 98:2 H2O/acetonitrile (v/v) and filtrated through a 0.2 µm spin filter (Merck Millipore Ultrafree®-MC ...
-
bioRxiv - Immunology 2021Quote: ... supplemented with 2 % (vol/ vol) FCS and and 10 mM 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES, Merck) and cut into 1 cm pieces ...
-
bioRxiv - Bioengineering 2021Quote: ... The chemical reagents of L-cysteine (analytical reagent) and lactic acid (analytical reagent) were purchased from Merck (Darmstadt, Germany) and Fischer scientific (Merelbeke ...
-
bioRxiv - Biochemistry 2019Quote: ... Samples were washed three times with 1X PBS and IgG was eluted using 0.1M formic acid (Merck, Darmstadt, Germany) followed by immediate neutralisation with 1M ammonium bicarbonate (Acros Organics ...
-
bioRxiv - Biochemistry 2020Quote: ... ammonia (25% in H2O), acetic acid (≥ 98.5%), ethanol (≥ 99.5%), and sodium hydrogencarbonate (NaHCO3, ≥ 99.5%) were purchased from Merck (Darmstadt, Germany). Tris(hydroxymethyl)amino-methane (TRIS ...
-
bioRxiv - Microbiology 2020Quote: ... The LC flow rate was 300 nL/min and the 100-min linear gradient ranged from 95% solvent A (H2O with 0.1% formic acid (Merck)) to 40% solvent B (100% acetonitrile with 0.1% formic acid) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... for 10 minutes at room temperature, Ponceau (C.I.14700, FUJIFILM-Wako Pure Chemical Corp., Osaka, Japan) acid fuchsin (C.I.42685, Merck-Millipore) azofloxine (C.I.18050 ...
-
bioRxiv - Cancer Biology 2022Quote: ... 400μL were mixed with 200μL of a 3 mg/mL collagen in 0.1% acetic acid solution (rat tail type I; Merck Millipore), followed by the addition of 10μL of 1 M NaOH ...
-
bioRxiv - Developmental Biology 2022Quote: ... Total protein concentration of lysates was determined using a bicinchoninic acid kit (Merck Life Science UK Ltd, Gillingham, UK) and samples diluted in Laemmli buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... pH 8.0) and then transferred to 1.5 mL tubes prewarmed at 65°C acid-washed glass beads (G8772-100 Merck) suspended in 25 µL of 10% filtered SDS and 300 µL of acid phenol chloroform pH 4.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... was incubated in 5 mM Tris-HCl buffer (pH 7.5) containing each substrate (1% glucomannan, Neogen, MI, USA; 1% polygalacturonic acid, Neogen; 1% carboxymethyl cellulose, Merck; 1% soluble starch ...
-
bioRxiv - Neuroscience 2024Quote: ... the brain sections were thawed in a desiccator and spray-coated with 80 µl of a freshly prepared solution of 2,5-dihydroxybenzoic acid (DHB, Merck) using a SMALDIPrep ultrafine pneumatic spraying system (TransMIT GmbH) ...
-
bioRxiv - Cell Biology 2022Quote: ... The total concentration of proteins in samples was determined using the Bicinchoninic Acid Assay Kit (Sigma Aldrich/Merck, Poznan, Polska). The samples containing 20 µg of protein were mixed with 4× Laemmli loading buffer (Bio-Rad ...
-
bioRxiv - Neuroscience 2021Quote: ... the cells were incubated in low serum medium (0.5% FBS) containing 20 µM retinoic acid (RA) (Sigma-Aldrich; Merck KGaA) for 5 days ...
-
bioRxiv - Immunology 2021Quote: ... The digested products were then diluted with formic acid to a final concentration of 0.1% and cleaned up using ZipTip C18 (Merck Millipore). Data was obtained on an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2020Quote: ... HPLC-grade ammonium formate (>98%) and formic acid (>98%) used in the LC mobile phase were purchased from Merck (Germany) and Sigma-Aldrich (USA) ...
-
bioRxiv - Systems Biology 2020Quote: ... The resultant pellets were resuspended in 3% acetonitrile + 0.1% trifluoroacetic acid and peptide quantification performed using the Direct Detect system (Merck Millipore). Protein samples were normalized then vacuum concentrated in preparation for mass spectrometry.
-
bioRxiv - Microbiology 2020Quote: ... the supernatant was then filtered through an acid-washed 0.22 μm polycarbonate filter (GSV, Life Science) using a Sterifil 47 mm filter holder (Merck Millipore). The flow-through liquid was transferred into an acid-washed 10 L polycarbonate (PC ...
-
bioRxiv - Cancer Biology 2020Quote: ... The cell pellets were digested with a standard volume of suprapur nitric acid and hydrogen peroxide (Merck Millipore, MA, USA) (2:1 v/v ...
-
bioRxiv - Microbiology 2020Quote: ... Samples were stored at 4°C overnight before desilicification with 4% suprapure hydrofluoric acid (Merck; incubation of approximately 5 hours). Afterwards ...
-
bioRxiv - Developmental Biology 2021Quote: ... 500 μg/mL rice-derived recombinant human albumin and 213 μg/mL L-ascorbic acid 2-phosphate (both from Merck). After 24 h of CHIR99021 stimulation ...
-
bioRxiv - Biochemistry 2021Quote: ... Reactions were stopped at each time point using 4 N formic acid and analyzed by PEI-Cellulose-F TLC (Merck) developed in 0.4 M potassium phosphate buffer (pH 3.4) ...
-
bioRxiv - Microbiology 2020Quote: ... A was 1 mM ammonium formate pH 9 (for lincomycin detection, prepared by titration of formic acid 98–100%, Merck, Germany with ammonium hydroxide 28–30% ...
-
bioRxiv - Immunology 2021Quote: ... neuraminidase-specific inhibitor DANA was added to a final concentration of 100 μM (N-Acetyl-2,3-dehydro-2-deoxyneuraminic acid, Merck, USA). It was used for 48 h prior stimulus.
-
bioRxiv - Microbiology 2022Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 µg/mL 2,6- Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
-
bioRxiv - Bioengineering 2022Quote: ... Nitric acid 65%, N,N-dimethylformamide (DMF), sodium hydroxide (NaOH) and Ethylenediamine (EDA, ≥99 %) were purchased from Merck (Darmstadt, Germany). Dulbecco’s modified Eagle’s medium ...