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Citations for Merck :
451 - 500 of 1644 citations for 4 4 Methylphenyl 1H pyrrole 3 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... the harvested tissues were immediately fixed in 4% (w/v) paraformaldehyde (PFA; Merck, Darmstadt, Germany) for 24 hours ...
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bioRxiv - Cell Biology 2021Quote: ... After treatment for the indicated time the cells were fixed in 4% paraformaldehyde (Merck, 100496,) and permeabilized with cold methanol at −20°C for 2 min or 0.1% TX-100 in PBS for 5 min at room temperature followed by blocking with 0.12% glycine (Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... 21,000 x g at 4°C and sterile-filtered using a 0.45µm PVDF-membrane (Merck).
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bioRxiv - Molecular Biology 2022Quote: ... Rad54 was concentrated using an Amicon Ultra-4 (50 k) centrifugal filter (UFC805008, Merck Millipore).
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bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore ...
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bioRxiv - Plant Biology 2024Quote: ... 4 mM phosphoenolpyruvate (PEP)) as well as 1.2 U pyruvate kinase (P1506; Sigma-Aldrich/Merck) were added to convert ADP and PEP to ATP and pyruvate ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... determined by means of quantitative NMR using TraceCERT® ethyl 4-(dimethylamino)benzoate from Merck as an internal calibrant [48 ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was then concentrated through an Amicon Ultra-4 Filter Unit (Merck, cat#UFC8100214). The validation of the cytoplasm fraction was performed by western blot using antibodies against markers for nucleus (H2A) ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies were applied overnight at 4°C (monoclonal anti–IdU 1:4000; SAB3701448, Merck). Sections were incubated in biotinylated secondary antibodies (Jackson ImmunoResearch Labs ...
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bioRxiv - Immunology 2024Quote: ... cells were treated for 4 h with 5 mg/ml Brefeldin A (Merck, Darmstadt, Germany) after 140 h incubation time ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing ELAC2 were concentrated using Amicon Ultra-4 30K Centrifugal Filter Devices (Merck Millipore), aliquoted ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.1% X-Gal (from a 4% X-Gal solution in dimethylformamide, Merck Life science). Buffer was passed through a 0.2 micron syringe filter before X-Gal was added ...
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bioRxiv - Cell Biology 2023Quote: ... sections were rehydrated and stained with haematoxylin (Fluka) for 4 min and with eosin (Merck) for 2 min ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were stained with Ponceau S and subsequently blocked in 4% BSA (A8022, Sigma/Merck) in 1x PBS-Tween (137 mM NACl ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µM trans- Epoxysuccinyl-L-leucylamido(4-guanidino)butan (E64, Sigma-Aldrich, Merck, Taufkirchen, Germany) was added ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed for 30 min at 4°C with 0.4 % paraformaldehyde (PFA, Merck KGaA) and permeabilized by washing twice with permeabilization buffer (PBS containing 2 % FBS and 0.1 % saponin (Sigma)) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The gelatinous sack surrounding the eggs was removed using 4% L-Cysteine (Merck Milipore, USA) and followed by microinjecting the zygotes with antisense MOs ...
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bioRxiv - Genetics 2023Quote: ... individual fibers were transferred onto a 4-well chamber slide (Nunc Lab-Tek, Merck, GmbH) containing HBSS with 2.5μM FM1-43 dye (Molecular Probes ...
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bioRxiv - Biochemistry 2023Quote: ... After the standard Ni-NTA purification process using Ni-NTA beads (Merck, cat. 70666-4) and subsequent Sumo protease digestion (concentration of sumo protease at 1:200) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then fixed and permeabilized using a combination of 4% paraformaldehyde (Sigma-Aldrich/Merck) and 0.1% Triton X-100 (Sigma-Aldrich/Merck) ...
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bioRxiv - Biochemistry 2023Quote: ... samples were concentrated at 3,500 rpm and 4°C using Amicon Ultra centrifugal units (Merck).
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bioRxiv - Neuroscience 2023Quote: ... and transcardially perfused with 0.9% NaCl followed by 4% paraformaldehyde (PFA, Sigma, Merck, Germany, P6148). The brains were harvested and post-fixed overnight in 4% PFA at 4°C ...
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bioRxiv - Immunology 2023Quote: ... permeabilized with 0.1% saponin in PBS and incubated with 4 μg/ml Hoechst 33342 (Merck) for 10 min at room temp ...
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bioRxiv - Biochemistry 2024Quote: ... and blocked overnight at 4°C with 1% bovine serum albumin (BSA, Merck, Cat# 7906) in PBS (200 µl per well) ...
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bioRxiv - Biochemistry 2024Quote: ... We then stimulated the cells for 4 h with 100 ng/ml PMA (524400; Merck) and 1 µg/ml ionomycin (407952 ...
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bioRxiv - Cell Biology 2024Quote: Cultured cells for 24 hours of incubation were fixed with 4% paraformaldehyde (PFA) (#104005, Merck) in Hank’s balanced salt solution containing 1 mM Ca2+ and Mg2+ ...
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bioRxiv - Microbiology 2024Quote: ... and used to transduce HeLa cells in the presence of 4 µg/ml polybrene (Merck). At 24 hours post-transduction ...
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bioRxiv - Systems Biology 2024Quote: ... UK) and separated by gel electrophoresis (7.5% acrylamide, 4 mg/ml porcine gelatin; Merck, UK). SDS was removed by washing in 50 mM Tris.HCl ...
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bioRxiv - Synthetic Biology 2024Quote: ... 500 µL 0.2 µg/mL 4’,6’-diamidino-2-phenylindole (DAPI, Merck, catalog no. D9542) in PBS was added for 15 min at RT ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% 2-mercaptoethanol at a 4:1 ratio (Merck, Sigma-Aldrich, cat. M6250) and heated at 55 °C for 7 min ...
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bioRxiv - Cell Biology 2024Quote: ... and separated on mPAGE™ 4-12% Bis-Tris Precast Gels (Merck, MP41G10 or MP41G12) through standard SDS-PAGE protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated for 1h in base assay medium (D5030, Sigma Aldrich, Merck, Darmstadt, Germany) supplemented with 2 mM glutamine ...
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bioRxiv - Immunology 2023Quote: ... MDMs were also pre-treated for 1h with a TLR3/dsRNA complex inhibitor (Merck Millipore) 5 µM final concentration ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with following primary antibodies at 4 °C overnight: TRA-1-60 (Merck Millipore), OCT3/4 (H-134 ...
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bioRxiv - Microbiology 2021Quote: Blocks of cells or tissue were incubated overnight in 2.3M sucrose at 4°C (Merck, K17687153) in 0.1M phosphate buffer ...
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bioRxiv - Bioengineering 2021Quote: ... CD14+-cells were detached in a similar manner: 4 mg/mL lidocaine hydrochloride (L5647; Merck KGaA) were solved in Versene Solution freshly for each detachment ...
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bioRxiv - Neuroscience 2021Quote: ... Stained sections were washed 4 times in PBS and then embedded using Aquatex (Merck Millipore, USA).
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bioRxiv - Synthetic Biology 2021Quote: ... the medium was removed and cells were fixed for 10 min in 4% formaldehyde solution (Merck). The fixation solution was removed and the cells were washed twice with nuclease-free H2O (nfH2O) ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were fixed with 4% paraformaldehyde (PFA) and permeabilized with 0.1% SDS (Merck Millipore, Burlington, MA). Primary antibodies were incubated for 1 h at RT a humid chamber ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 ml of each sample were concentrated with Amicon ultra Centrifugal filters (Cat no. UFC510024, Merck). Samples were centrifuged (14 000 × g ...
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bioRxiv - Neuroscience 2022Quote: ... cells nuclei were stained with DAPI (4′,6-diamidino-2-fenilindol, 1:10000, Merck, cat#D9542) for 5 minutes at RT and mounted in Lab Vision™ PermaFluor™ Aqueous Mounting Medium (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... concentrated with Amicon Ultra-4 Centrifugal Filter Unit (100 kDa cut-off, Merck Millipore, Cat#UFC810024) to ~1 mg/mL ...
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bioRxiv - Biochemistry 2020Quote: Purified proteins were concentrated using Amicon® Ultra-4 Ultracel®-10K (MWCO: 10.000 Da; Merck). Protein concentrations were quantified using the calculated molar extinction coefficient ε280 (29).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The resulting ultrafiltrate (15min 14000g 15°C) was acidified with 20 µl 4% formic acid (Merck) in dH2O ...
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bioRxiv - Biochemistry 2020Quote: ... and anti-integrin β1 antibody (clone HUTS-4) was obtained from Chemicon (Merck Millipore, Billerica, MA). Rabbit polyclonal antibodies to fibronectin and fibrinogen were from Abcam (Cambridge ...
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bioRxiv - Developmental Biology 2021Quote: ... iPSC-derived mesodermal cells were fed with basal medium supplemented with 4 μM IWR-1 (Merck) for 48 h ...
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bioRxiv - Neuroscience 2021Quote: ... Fractions of interest were concentrated using 10 kDa cut-off Amicon Ultra-4 concentrators (Merck Millipore) before loading on a Superdex 200 10/300 GL (Cytiva ...
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bioRxiv - Neuroscience 2021Quote: ... 400 μl cell lysate were incubated with 4 μl HTT MAB2166 rabbit primary antibody (Merck, #MAB2166) for 2 hours at 4° C on a rotating device ...
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bioRxiv - Neuroscience 2021Quote: ... or Cy5-conjugated secondary antibodies (1:200; AP192SA6; Merck Millipore, USA; 17 hrs at 4°C). Similar immunolabeling steps were followed for the subsequent sequential staining ...
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bioRxiv - Immunology 2020Quote: ... naïve T cells were freshly isolated and subsequently cultured for 4/5 days in RPMI (Merck), containing 10% Heat Inactivated FCS (Invitrogen ...